Species confirmation of freshwater prawns in Ternate Island, Indonesia, through DNA barcoding: Not Macrobrachium rosenbergii Muhammad Nur Findra, Gamal M Samadan, Supyan, Aras Syazili, Muhammad Irfan International Journal of Aquatic Biology, 2025 Freshwater prawns of the genus Macrobrachium are widely distributed across tropical and subtropical regions. On Ternate Island, the local community refers to native freshwater prawns as “udang galah”, a name typically associated with Macrobrachium rosenbergii. This study aimed to accurately identify freshwater prawn species on Ternate Island using DNA barcoding. Prawn samples were collected from two different locations on Ternate Island: Togafo River, which has a steep gradient and flows directly into the sea without being affected by tidal salinity, and the river in Fitu Village, which consists of isolated pools with no connection to marine waters. Tissue samples were taken and preserved in 96% alcohol for molecular analysis. DNA barcoding results confirmed that the identified species is Macrobrachium lar, with genetic similarity ranging from 99.50% to 99.83% to reference data in GenBank. The presence of M. lar in isolated freshwater habitats raises questions about its dispersal patterns and life cycle, as this species is amphidromous and typically requires brackish water for larval development. These findings suggest the possibility of local adaptation or alternative dispersal mechanisms that warrant further investigation. Additionally, this study highlights the effectiveness of DNA barcoding for species identification, particularly for Macrobrachium species with high morphological variation or classified as cryptic.
Application of Various Liquid Organic Fertilizers in Spurring the Growth of Seaweed Gracilaria sp. Muhammad Irfan, Gamal M. Samadan, Nursanti Abdullah, Yuliana Yuliana, Sudirto Malan Egyptian Journal of Aquatic Biology and Fisheries, 2025 Liquid organic fertilizers offer several advantages, including rapid correction of nutrient deficiencies, minimal nutrient loss, and quick nutrient availability. This study is expected to contribute to the development of seaweed cultivation by highlighting the importance of liquid organic fertilizer application in enhancing the growth of Gracilaria sp. The liquid organic fertilizers used in this study were derived from cow, goat, and chicken manure collected from farmers in Ternate City. Prior to use, the manure was dried for three days. Each type of organic fertilizer was then weighed at 1kg and diluted with 1L of water, followed by thorough mixing until a liquid solution was formed. From each solution, a dose of 30mL was taken for use in soaking Gracilaria sp. The liquid organic fertilizer was then applied by spraying into the maintenance containers containing Gracilaria sp. Spraying was conducted three times daily, in the morning, afternoon, and evening. The data analyzed included absolute weight growth, calculated as the difference between the average final weight at the end of the maintenance period and the initial weight at stocking, as well as the relative growth rate. Based on the results of this study, it can be concluded that the application of different types of liquid organic fertilizers did not have a significant effect on absolute weight growth or relative growth rate of Gracilaria sp. However, liquid organic fertilizer derived from cow manure produced the best growth performance in terms of both absolute weight and relative growth.
Characteristics of 16S rRNA gene in seahorses from Ternate Island waters, Indonesia Aacl Bioflux, 2024
Genetic characteristics of Macrobrachium lar from Gane Timur, Halmahera Island, Indonesia, based on mitochondrial COI gene Aacl Bioflux, 2024
Ginger (Zingiber officinale Roscoe) flour diet in gourami (Osphronemus gourami) hatchery with biofloc system Aacl Bioflux, 2022
The effectiveness of sand and red tilapia rearing in absorbing nitrogen and phosphorus of liquid waste from Litopenaeus vannamei culture Aacl Bioflux, 2022
Traditional wisdom as a starting point for conservation: A review Supyan, A N Susanto, G M Samadan, Sulistiono Iop Conference Series Earth and Environmental Science, 2021 Traditional wisdom was established on familiarity and concern for the homeland, where people are very dependent on their local resources and they developed management values based on their cultural beliefs. Traditional wisdom and its application can be useful for ecological management plans, especially conservation programs. The application of traditional wisdom as a starting point for conservation is based on: (1) Community institutional system (2) Community collective knowledge (3) Community relationship with their environment. Traditional wisdom is part of the basic theoretical framework in strengthening research designs with specific local knowledge, including environmental relationships that occur in the area. When conservationists recognize usefulness of traditional wisdom, they can engage in knowledge exchange and foster sharing of responsibilities with indigenous peoples. This type of exchange can also provide opportunities for indigenous peoples to develop scientific infrastructure.
Effects of different times of probiotic additions on floc abundance and growth of white leg shrimp (Litopenaeus vannamei): Laboratory scale cultivation G M Samadan, Yuliana, R Masril, A Syazili, Supyan Iop Conference Series Earth and Environmental Science, 2021 Water quality management for aquaculture is very important because water is a living medium for aquaculture organisms. One of the technological products that use microbes to create a better environment by breaking down organic matter is probiotics. This study aims to determine the effects of different probiotic administration times on water quality in white leg shrimp culture at a laboratory scale. Juveniles of white leg shrimp with PL27 size were reared for 45 days and given four treatments with probiotics at different times. The result showed that the administration of probiotics with a short period interval had a significant effect on floc volume, absolute growth, survival rate, concentrations of ammonia, nitrate, and nitrite. Provision of probiotics with a short time span tended to reduce the concentration of ammonia, nitrate and nitrite. The difference in time of giving probiotics to bio-floc media increased the growth of white leg shrimp, improved water quality, and reduced the concentration of ammonia (NH3), nitrate (NO3), and nitrite (NO2).
Growth and immunity of african catfish (Clarias gariepinus) with dietary inclusion of ginger (zingiber officinalis) and turmeric (curcuma domestica) Aacl Bioflux, 2021
Induction of Hsp60, Hsp70, and Hsp90 in formalin exposed white shrimp (Litopenaeus vannamei) stress test N S Yasa, L Anshory, S J. Purnomo, G M Samadan Iop Conference Series Earth and Environmental Science, 2020 Exposure to formalin was investigated as possible stress tests for evaluating the quality of white shrimp (L. vannamei) PLs. For the formalin stress test, PL10 was stocked in 300 ml cup with seawater of 0 (control), 300, and 600 mg/L formalin for the solution test. The PLs from the same batches were used for seed production to monitor their performance and validate the results of stress tests. Exposure of post larvae to 600 mg/L formalin for 3 hr appeared to be a reliable and practical method for post-larval quality assessment as the survival of larvae in the mass production tanks validated the classification of good and poor qualities batches in the stress tests. On this basis, a hatchery operator can decide which batch should be cultured further. Finally, there appears to be a link between the mortality of PLs and the expression of heat shock proteins. HSP are a highly conserved protein sequence available in the prokaryotic and eukaryotic organism that is known for its rapid response to environmental stresses. During the formalin stress test, the Hsp60 showed the highest expression in comparison with Hsp70 and Hsp90.
Production performance of whiteleg shrimp Litopenaeus vannamei at different stocking densities reared in sand ponds using plastic mulch Aacl Bioflux, 2018
