Eman Hany

@mans.edu.eg

Oral Biology department
Oral Biology department, Faculty of Dentistry, Mansoura University

Eman Hany


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https://researchid.co/emanhany
10

Scopus Publications

90

Scholar Citations

6

Scholar h-index

3

Scholar i10-index

Scopus Publications

  • Impact of two different hesperidin forms loaded on nanoscale modified borate bioglass scaffolds on rat critical-sized calvarial defects
    Nada A. Alqiran, A. M. Abdelghany, Samah Fouad, Mohammed E. Grawish, Yousry M. Elhawary, et al.
    Scientific Reports, 2026
  • Photobiostimulation Enhances the Osteogenic Differentiation of Gingival-Derived Stem Cells Seeded on Nanocomposite Scaffolds
    Eman Hany, Ahmed A. Emam, Mohamed G. Elbeltagy, Mahmoud M. Zakaria, Sarah Yahia, et al.
    Regenerative Engineering and Translational Medicine, 2025
  • Comparison of the osteoinductive potential of preconditioned dental pulp stem cell secretome versus their exosomes
    Eman Hany, Ahmed A. Emam, Dina Elzeiny, Ahmed A. Elzeiny, Rana El-Qashty
    Odontology, 2025
    This study aimed to compare the effects of osteogenically preconditioned dental pulp stem cells (DPSCs) secretome versus their exosomes on proliferation, migration, osteogenic differentiation, and mineralization of bone marrow stem cells (BMSCs). BMSCs and DPSCs were isolated and characterized. DPSCs were osteogenically preconditioned for 10 days before isolation of their derivatives. The study involved four groups: negative control (BMSCs in complete media), positive control (BMSCs in complete media with osteogenic supplement), secretome group (BMSCs co-cultured with DPSCs-secretome), and exosome group (BMSCs co-cultured with DPSCs-exosomes). BMSCs’ viability through MTT assay and cell migration were evaluated. Mineralization was assessed through Alizarin red S and Von Kossa staining. RT-qPCR was performed for osteogenic gene expression, followed by statistical analysis. The exosome group showed the highest cell proliferation on day 5, but it did not reach statistical significance. Cell migration assay showed more rapid wound closure in the control group. However, after 24 h, the secretome group outperformed the exosome group, while the opposite results were observed after 48 h. The exosome group revealed the highest mineralization and osteogenic marker expressions, followed by the secretome, and finally the control groups, with significant differences between groups. Therefore, osteogenic preconditioning can be considered as an efficient tool for enhancing the osteoinductive influence of DPSCs-derivatives, promoting osteoblastic differentiation and mineralization, and under the tested conditions, exosomes demonstrated greater osteoinductive activity compared to secretome. However, osteogenically committed cells seem to lose their paracrine influence on cell viability and migration. Graphical Abstract
  • The role of erythropoietin-loaded hydrogel versus adipose derived stem cell secretome in the regeneration of tongue defects
    Rana El-Qashty, Jilan Youssef, Eman Hany
    BMC Oral Health, 2024
    Background Tongue defects have several etiologies and significantly affect the quality of life. This study was conducted to compare the regenerative potential of erythropoietin (EPO)-loaded hydrogel and adipose derived stem cell (ADSC) secretome on tongue dorsum defects focusing on the role of anti-inflammatory M2 macrophage phenotype. Methods Rats were subjected to induction of mechanical circular defects on the dorsal surface of the tongue, then divided into three groups; Group I (control): received 0.1 ml phosphate buffered saline, Group II (EPO): received 5000 U/kg EPO-hydrogel, and Group III (ADSC-Secretome): received 0.1 ml ADSC-Secretome. Treatments were injected circumferentially around wound margins after induction. Seven and fourteen days after treatment, specimens were obtained and processed for histological and immunohistochemical staining followed by the relevant histomorphometric and statistical analyses. Results Seven days after treatment, groups II and III presented defects with some epithelial regeneration at the lateral margins, while the center of the defect showed granulation tissue with much inflammatory cells. The base of the defects showed some muscle fibers and new blood vessels, however group III showed more enhanced neovascularization. Fourteen days after therapeutic intervention, group II defects were completely covered with epithelium showing a thin keratin layer with regular rete pegs interdigitating with the underlying connective tissue papillae, but tongue papillae were not restored. Group III expressed much better healing with developing filiform papillae. The connective tissue showed more vascularity and well-arranged muscle bundles. Both treated groups showed a significant decrease in defect depth and significant increase in anti-inflammatory macrophages compared to the control group at both time intervals, however there was no significant difference between the two treated groups. Conclusion Both treatments showed promising and comparable results in the treatment of tongue defects reducing inflammation and restoring tongue histological architecture with significant upregulation of M2 macrophage.
  • The Regenerative Potential of Adipose-Derived Stem Cells Versus their Conditioned Media on Tongue Defects of Adult Male Rats
    Eman Hany, Ayman Elkashty, Rana El-Qashty
    Egyptian Journal of Histology, 2024
  • Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model
    Rana El-Qashty, Osama A. Elkashty, Eman Hany
    BMC Oral Health, 2023
    Background Temporomandibular joint (TMJ) arthritis is a debilitating, challenging condition and different methods have been implicated for its treatment. This study aimed to test the therapeutic potentials of low-level laser therapy (LLLT) associated with adipose derived stem cells (ADSC) or their derived secretome on a murine model induced arthritis. Methods Forty eight rats were divided into four groups where group I was the sham control, the rest of animals were subjected to arthritis induction using complete Freund’s adjuvant, then divided as follows: group II received phosphate buffered saline (PBS) intraarticular injection and irradiation of 0 j/cm2, group III received ADSCs derived secretome and irradiation of 38 j/cm2, and group IV received ADSCs and irradiation of 38 j/cm2 as well. One and three weeks after treatment, animals were euthanized, and paraffin blocks were processed for histological assessment by hematoxylin and eosin stain with histomorphometrical analysis. Histochemical evaluation of joint proteoglycan content was performed through toluidine blue stain, and immunohistochemical staining by the proinflammatory marker tumor necrosis factor-α (TNF-α) was performed followed by the relevant statistical tests. Results The arthritis group showed histological signs of joint injury including cartilage atrophy, articular disc fibrosis, irregular osteochondral interface, and condylar bone resorption together with high inflammatory reaction and defective proteoglycan content. In contrast, the treated groups III and IV showed much restoration of the joint structure with normal cartilage and disc thickness. The inflammation process was significantly suppressed especially after three weeks as confirmed by the significant reduction in TNF-α positive immunostaining compared to the arthritic group, and the cartilage proteoglycan content also showed significant increase relative to the arthritic group. However, no significant difference between the results of the two treated groups was detected. Conclusion LLLT conjugated with ADSCs or ADSCs derived secretome can efficiently enhance the healing of arthritic TMJs. Stem cell secretome can be applied as a safe, potent therapy. However, further investigations are required to unravel its mechanism of action and pave its way as a safe, novel, cell free therapy. Graphical Abstract
  • Characterization of a nanocomposite scaffold and assessment of its osteogenic influence in a rabbit mandibular bone defect model
    Eman Hany, Noha El-Wassefy, Sarah Yahia, Mazen Abou Elkhier, Ibrahim M. El-Sherbiny
    Journal of Oral and Maxillofacial Surgery Medicine and Pathology, 2023
  • Evaluation of the osteogenic potential of rat adipose-derived stem cells with different polycaprolactone/alginate-based nanofibrous scaffolds: an in vitro study
    Eman Hany, Sarah Yahia, Mahmoud Fathy Elsherbeny, Nagla Mahmoud Salama, Islam Mohammed Ateia, et al.
    Stem Cell Investigation, 2020
    Background Bone tissue engineering is a widely growing field that requires the combination of cells, scaffolds and signaling molecules. Adipose derived stem cells (ADSCs) are an accessible and abundant source of mesenchymal stem cells with high plasticity. Polycaprolactone/alginate (PCL/Alg) composite scaffolds have been used in bone regeneration and nano-hydroxyapatite (n-HA) is used as a reinforcing, osteoconductive component in scaffold fabrication. This study was conducted to assess the ability of three different PCL/Alg based scaffolds to induce osteogenic differentiation of ADSCs and to compare between them. Methods The study comprised 5 groups; negative control group with ADSCs cultured in complete culture media, positive control group with ADSCs cultured in osteogenic differentiation media, and 3 experimental groups with ADSCs seeded onto 3 scaffolds: S1 (PCL/Alg), S2 (PCL/Alg/Ca) and S3 (PCL/Alg/Ca/n-HA) respectively and cultured in osteogenic media. Mineralization and gene expression were assessed by Alizarin red S (ARS) staining and real time quantitative polymerase chain reaction (RT-qPCR). Evaluation was done at 7, 14 and 21 days. Results ARS staining reflected a time dependent increase through days 7, 14 and 21, with S3 (PCL/Alg/Ca/n-HA) group showing the highest mineralization levels. RT-qPCR detected upregulation of ALP gene expression at day 7 and decline thereafter. S2 (PCL/Alg/Ca) and S3 (PCL/Alg/Ca/n-HA) groups showed significantly higher gene expression levels than S1 (PCL/Alg). Conclusions ADSCs and PCL/Alg-based scaffolds compose a good tissue engineering complex for bone regeneration. Addition of n-HA to PCL/Alg scaffolds and crosslinking with CaCl2 efficiently improve the osteogenic potential of ADSCs.
  • Emerging therapeutic modality enhancing the efficiency of chemotherapeutic agents against head and neck squamous cell carcinoma cell lines
    Mohammed E. Grawish, Mohamed I. Mourad, Doaa AM Esmaeil, Rehab A Ahmed, Islam Mohamed Ateia, et al.
    Cancer Treatment and Research Communications, 2020
    The current work aimed to evaluate bee venom (BV) cytotoxic effect and its synergistic action when combined with cisplatin (CIS) against four types of head and neck squamous cell carcinoma (HNSCC) cell lines. 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) assay for cell viability, reverse transcription-polymerase chain reaction (RT-PCR) for expression of BCL2 associated X (BAX), B-cell lymphoma 2 (BCL2) and epidermal growth factor receptor (EGFR) genes and, flow cytometry for cell cycle analysis were performed. MTT assay revealed that BV caused an approximately 50% cell death for UMSCC12, UMSCC29, UMSCC38 and, UMSCC47 cell lines after 72 hr with 54.809 µg/ml, 61.287 µg/ml, 71.328 µg/ml and, 61.045 µg/ml, respectively. RT-PCR demonstrated a significant up-regulation of BAX gene and a significant down-regulation of BCL2 and EGFR genes among single or combined treatments with CIS and BV as compared to vehicle-treated. The cell lines treated with both BV and CIS showed marked elevation of BAX and a notable drop of BCL2 and EGFR expressions than single-treated groups. Cell cycle analysis via flow cytometry revealed significantly increased cells in the G2/M phase in single or combined-treated cell lines with CIS and BV when compared with vehicle-treated. Moreover, a significant decrease in cells in S phases among all single and combined treatments when matched with vehicle-treated. Briefly, the findings of the present study suggest that BV can exert an anti-cancer effect on HNSCC and may have the potentiality for potentiation of CIS cytotoxic effects and reduction of its adverse effects.
  • The effect of different routes of injection of bone marrow mesenchymal stem cells on parotid glands of rats receiving cisplatin: A comparative study
    Eman Hany, Mohammed A. Sobh, Mazen T. Abou ElKhier, Heba M. ElSabaa, Ahmed R. Zaher
    International Journal of Stem Cells, 2017
    Background and Objectives Cisplatin is a powerful antitumor chemotherapeutic agent that is widely used in the treatment of many cancers but it has many side effects on many organs including salivary glands. Bone marrow is considered to be a rich environment that comprises many types of stem cells of which BMSCs (Bone marrow mesenchymal stem cells) are the most studied with potentiality to differentiate into many cell types. This study was conducted to evaluate the effect of different routes of injection of BMSCs on parotid glands of rats receiving cisplatin. Methods and Results Sprague-Dawley rats were divided into 3 groups: a negative control group receiving phosphate buffered saline, a positive control group receiving cisplatin, and an experimental group where rats received cisplatin and then received iron oxide-labeled BMSCs by either intravenous or intraparotid routes or both. Animals were sacrificed at periods of 3,6,10 and 15 days after cisplatin injection, then histological, ultrastructural and immunohistochemical studies were done. The experimental stem cell treated group showed better histological features and increased PCNA proliferation index when compared to the control. The systemic and combination groups showed better results than the local group. Iron oxide-labeled cells were detected with Prussian blue stain. Conclusions This study proved that BMSCs can improve cisplatin induced cytotoxicity in parotid glands. Systemic administration showed to have a better effect than local intraparotid administration and comparable effect to combined administration.

RECENT SCHOLAR PUBLICATIONS

  • Impact of two different hesperidin forms loaded on nanoscale modified borate bioglass scaffolds on rat critical-sized calvarial defects
    NA Alqiran, AM Abdelghany, S Fouad, ME Grawish, YM Elhawary, E Hany
    Scientific Reports , 2026
    2026
  • Comparison of the osteoinductive potential of preconditioned dental pulp stem cell secretome versus their exosomes
    E Hany, AA Emam, D Elzeiny, AA Elzeiny, R El-Qashty
    Odontology, 1-15 , 2025
    2025
  • Photobiostimulation enhances the osteogenic differentiation of gingival-derived stem cells seeded on nanocomposite scaffolds
    E Hany, AA Emam, MG Elbeltagy, MM Zakaria, S Yahia, IM El-Sherbiny, ...
    Regenerative Engineering and Translational Medicine 11 (1), 190-208 , 2025
    2025
    Citations: 2
  • The role of erythropoietin-loaded hydrogel versus adipose derived stem cell secretome in the regeneration of tongue defects
    R El-Qashty, J Youssef, E Hany
    BMC Oral Health 24 (1), 1109 , 2024
    2024
    Citations: 3
  • The regenerative potential of adipose-derived stem cells versus their conditioned media on tongue defects of adult male rats
    E Hany, AAR Elkashty, R El-Qashty
    Egyptian Journal of Histology 47 (1), 253-267 , 2024
    2024
    Citations: 9
  • Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model
    R El-Qashty, OA Elkashty, E Hany
    BMC Oral Health 23 (1), 720 , 2023
    2023
    Citations: 22
  • Therapeutic potential of low-level laser photobiomodulation on adjuvant induced temporomandibular joint arthritis in a rat model
    R El-Qashty, OA Elkashty, E Hany
    Mansoura Journal of Dentistry 10 (1), 1-8 , 2023
    2023
    Citations: 4
  • Characterization of a nanocomposite scaffold and assessment of its osteogenic influence in a rabbit mandibular bone defect model
    E Hany, N El-Wassefy, S Yahia, M Abou Elkhier, IM El-Sherbiny
    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 35 (1), 76-84 , 2023
    2023
    Citations: 6
  • The controversial role of MDM2, MCM3 and Bcl-X in Ameloblastoma and Ameloblastic Carcinoma: An update
    N Salama, E Hany, D Esmaeil
    Egyptian Dental Journal 68 (1), 405-418 , 2022
    2022
  • Evaluation of the osteogenic potential of rat adipose-derived stem cells with different polycaprolactone/alginate-based nanofibrous scaffolds: an in vitro study
    E Hany, S Yahia, MF Elsherbeny, NM Salama, IM Ateia, NT Abou El-Khier, ...
    Stem cell investigation 7, 14 , 2020
    2020
    Citations: 16
  • Emerging therapeutic modality enhancing the efficiency of chemotherapeutic agents against head and neck squamous cell carcinoma cell lines
    ME Grawish, MI Mourad, DAM Esmaeil, RA Ahmed, IM Ateia, E Hany, ...
    Cancer Treatment and Research Communications 25, 100242 , 2020
    2020
    Citations: 7
  • The effect of different routes of injection of bone marrow mesenchymal stem cells on parotid glands of rats receiving cisplatin: a comparative study
    E Hany, MA Sobh, MT Abou ElKhier, HM ElSabaa, AR Zaher
    International journal of stem cells 10 (2), 169-178 , 2017
    2017
    Citations: 19
  • The effect of salbutamol on the parotid salivary gland of albino rats (immunohistochemical study)
    EH Metwally, MA Mohammed, MA Grawish, YM El-Hawary
    Res J Med Sci 7 (1), 13-9 , 2013
    2013
    Citations: 2

MOST CITED SCHOLAR PUBLICATIONS

  • Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model
    R El-Qashty, OA Elkashty, E Hany
    BMC Oral Health 23 (1), 720 , 2023
    2023
    Citations: 22
  • The effect of different routes of injection of bone marrow mesenchymal stem cells on parotid glands of rats receiving cisplatin: a comparative study
    E Hany, MA Sobh, MT Abou ElKhier, HM ElSabaa, AR Zaher
    International journal of stem cells 10 (2), 169-178 , 2017
    2017
    Citations: 19
  • Evaluation of the osteogenic potential of rat adipose-derived stem cells with different polycaprolactone/alginate-based nanofibrous scaffolds: an in vitro study
    E Hany, S Yahia, MF Elsherbeny, NM Salama, IM Ateia, NT Abou El-Khier, ...
    Stem cell investigation 7, 14 , 2020
    2020
    Citations: 16
  • The regenerative potential of adipose-derived stem cells versus their conditioned media on tongue defects of adult male rats
    E Hany, AAR Elkashty, R El-Qashty
    Egyptian Journal of Histology 47 (1), 253-267 , 2024
    2024
    Citations: 9
  • Emerging therapeutic modality enhancing the efficiency of chemotherapeutic agents against head and neck squamous cell carcinoma cell lines
    ME Grawish, MI Mourad, DAM Esmaeil, RA Ahmed, IM Ateia, E Hany, ...
    Cancer Treatment and Research Communications 25, 100242 , 2020
    2020
    Citations: 7
  • Characterization of a nanocomposite scaffold and assessment of its osteogenic influence in a rabbit mandibular bone defect model
    E Hany, N El-Wassefy, S Yahia, M Abou Elkhier, IM El-Sherbiny
    Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology 35 (1), 76-84 , 2023
    2023
    Citations: 6
  • Therapeutic potential of low-level laser photobiomodulation on adjuvant induced temporomandibular joint arthritis in a rat model
    R El-Qashty, OA Elkashty, E Hany
    Mansoura Journal of Dentistry 10 (1), 1-8 , 2023
    2023
    Citations: 4
  • The role of erythropoietin-loaded hydrogel versus adipose derived stem cell secretome in the regeneration of tongue defects
    R El-Qashty, J Youssef, E Hany
    BMC Oral Health 24 (1), 1109 , 2024
    2024
    Citations: 3
  • Photobiostimulation enhances the osteogenic differentiation of gingival-derived stem cells seeded on nanocomposite scaffolds
    E Hany, AA Emam, MG Elbeltagy, MM Zakaria, S Yahia, IM El-Sherbiny, ...
    Regenerative Engineering and Translational Medicine 11 (1), 190-208 , 2025
    2025
    Citations: 2
  • The effect of salbutamol on the parotid salivary gland of albino rats (immunohistochemical study)
    EH Metwally, MA Mohammed, MA Grawish, YM El-Hawary
    Res J Med Sci 7 (1), 13-9 , 2013
    2013
    Citations: 2
  • Impact of two different hesperidin forms loaded on nanoscale modified borate bioglass scaffolds on rat critical-sized calvarial defects
    NA Alqiran, AM Abdelghany, S Fouad, ME Grawish, YM Elhawary, E Hany
    Scientific Reports , 2026
    2026
  • Comparison of the osteoinductive potential of preconditioned dental pulp stem cell secretome versus their exosomes
    E Hany, AA Emam, D Elzeiny, AA Elzeiny, R El-Qashty
    Odontology, 1-15 , 2025
    2025
  • The controversial role of MDM2, MCM3 and Bcl-X in Ameloblastoma and Ameloblastic Carcinoma: An update
    N Salama, E Hany, D Esmaeil
    Egyptian Dental Journal 68 (1), 405-418 , 2022
    2022