MANAS RANJAN SAHOO
@icar.gov.in
Principal Scientist (Horticulture)
ICAR-Indian Institute of Horticultural Research (Central Horticultural Experiment Station)
RESEARCH INTERESTS
Biotic and Abiotic stress tolerance in vegetables
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Ingudam Bhupenchandra, Anjali Basumatary, Samiron Dutta, Anup Das, Anil K. Choudhary, Rattan Lal, A. Dhanachandra Sharma, Arnab Sen, Yumnam Prabhabati, and Manas Ranjan Sahoo
Elsevier BV
Ingudam Bhupenchandra, Sunil Kumar Chongtham, Ayam Gangarani Devi, Pranab Dutta, Elangbam Lamalakshmi, Sansuta Mohanty, Anil K. Choudhary, Anup Das, Konsam Sarika, Sumit Kumar,et al.
Wiley
AbstractA relative of cultivated rice (Oryza sativa L.), weedy or red rice (Oryza spp.) is currently recognized as the dominant weed, leading to a drastic loss of yield of cultivated rice due to its highly competitive abilities like producing more tillers, panicles, and biomass with better nutrient uptake. Due to its high nutritional value, antioxidant properties (anthocyanin and proanthocyanin), and nutrient absorption ability, weedy rice is gaining immense research attentions to understand its genetic constitution to augment future breeding strategies and to develop nutrition‐rich functional foods. Consequently, this review focuses on the unique gene source of weedy rice to enhance the cultivated rice for its crucial features like water use efficiency, abiotic and biotic stress tolerance, early flowering, and the red pericarp of the seed. It explores the debating issues on the origin and evolution of weedy rice, including its high diversity, signalling aspects, quantitative trait loci (QTL) mapping under stress conditions, the intricacy of the mechanism in the expression of the gene flow, and ecological challenges of nutrient removal by weedy rice. This review may create a foundation for future researchers to understand the gene flow between cultivated crops and weedy traits and support an improved approach for the applicability of several models in predicting multiomics variables.
Sansuta Mohanty, Bandana Kumari Mishra, Madhumita Dasgupta, Gobinda Chandra Acharya, Satyapriya Singh, Ponnam Naresh, Shyamlal Bhue, Anshuman Dixit, Arup Sarkar, and Manas Ranjan Sahoo
Springer Science and Business Media LLC
AbstractEggplant or aubergine (Solanum melongena L.) and its wild cousins, comprising 13 clades with 1500 species, have an unprecedented demand across the globe. Cultivated eggplant has a narrow molecular diversity that hinders eggplant breeding advancements. Wild eggplants need resurgent attention to broaden eggplant breeding resources. In this study, we emphasized phenotypic and genotypic discriminations among 13 eggplant species deploying chloroplast–plastid (Kim matK) and nuclear (ITS2) short gene sequences (400–800 bp) at DNA barcode region followed by ITS2 secondary structure predictions. The identification efficiency at the Kim matK region was higher (99–100%) than in the ITS2 region (80–90%). The eggplant species showed 13 unique secondary structures with a central ring with various helical orientations. Principal component analysis (PCoA) provides the descriptor–wise phenotypic clustering, which is essential for trait–specific breeding. Groups I and IV are categorized under scarlet complexes S. aethiopicum, S. trilobatum, and S. melongena (wild and cultivated). Group II represented the gboma clade (S. macrocarpon, S. wrightii, S. sisymbriifolium, and S. aculeatissimum), and group III includes S. mammosum, and S. torvum with unique fruit shape and size. The present study would be helpful in genetic discrimination, biodiversity conservation, and the safe utilization of wild eggplants.
Leimapokpam Tikendra, Hamidur Rahaman, Abhijit Dey, Manas Ranjan Sahoo, and Potshangbam Nongdam
Springer Nature Singapore
Satyapriya Singh, Mahadevan Raghuraman, Manikyanahalli Chandrashekara Keerthi, Anup Das, Saswat Kumar Kar, Biswajit Das, Hidangmayum Lembisana Devi, Sunil Kumar Sunani, Manas Ranjan Sahoo, Ryan Casini,et al.
MDPI AG
The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is a polyphagous non-native pest identified as a serious threat to crop production and food security globally, including in India. Its unintentional introduction and quick coverage in large areas is a serious concern to millions of farmers in the eastern Himalayan region. However, detailed understanding of farmers’ perceptions and the biological attributes associated with the meteorological factors for FAW is limited. The present investigation, which aimed to create baseline data on this pest, concurs with the idea that the FAW is widely dispersed throughout the maize ecosystems of Tripura, with an average infestation rate of 21.33 percent. The severity ranged from 0 to 1.40, with an average leaf damage score of 1 on a 0–4 categorical scale. The findings indicate that pheromone trap catch was significantly correlated with the evaporation rate as other meteorological factors influenced variably. The biological attributes imply that the life cycle was completed in 32.82 ± 0.08 days, with a high fecundity potential (1068.57 ± 4.35 numbers) in controlled conditions (25 ± 1 °C, 70 ± 5% RH). Indigenous natural enemies, such as entomopathogens, spiders, and wasps, were found to be the first defence against this invasive pest. A minority of the population (17.51%) was aware of the incidence of FAW. Furthermore, respondents’ socio-demographic variables were associated significantly with FAW status. This is the first scientific report from the eastern Himalayan region about farmers’ knowledge and awareness of the invasiveness of FAW. This finding enumerates a detailed understanding of FAW from diverse perspectives. Further, the concerted data provide an important baseline that could help the development of holistic management strategies for FAW.
Tanmaya Kumar Bhoi, Ipsita Samal, Deepak Kumar Mahanta, J. Komal, Dinesh Jinger, Manas Ranjan Sahoo, Gobinda Chandra Achary, Priyanka Nayak, Sunil Kumar Sunani, Varun Saini,et al.
Springer Science and Business Media LLC
Elizabeth Devi Wahengbam, Chingakham Premabati Devi, Susheel Kumar Sharma, Subhra Saikat Roy, Albert Maibam, Madhumita Dasgupta, Star Luikham, Tania Chongtham, Arati Ningombam, Ingudam Bhupenchandra,et al.
Frontiers Media SA
Rapid postharvest physiological deterioration (PPD) in cassava (Manihot esculenta Crantz) tuber is a significant concern during storage. The freshly harvested tubers start spoiling within 24 to 72 h. Accumulation of H2O2 is one of the earliest biochemical events that occurred during PPD, which was detected using the 3,3 diaminobenzidine (DAB) in two contrast cassava genotypes, MNP Local A (29–57 μg g–1) and Sree Prakash (64–141 μg g–1). Accumulating the fluorescence hydroxycoumarin compounds emitted by the cassava tubers observed under an ultraviolet (UV) lamp showed significant variations at 0, 3, 6, 9, 12, and 15 days of storage. The total phenolics and carotenoids significantly and negatively correlated with PPD progression; however, the anthocyanin and flavonoids positively correlated with the PPD–anchored ROS accumulation. The primary compound, Phthalic acid, di(2–propylpentyl) ester, was identified in both the cassava tubers, Sree Prakash (57.21 and 35.21%), and MNP Local A (75.58 and 60.21%) at 0, and 72 h of PPD, respectively. The expression of PPD–associated genes APX–2, APX–3, PAL, and AP was higher at 6–12 days of PPD, which signified the synthesis of ROS turnover and phenylpropanoid biosynthesis. A significant, strong, and positive correlation was established between the secondary metabolites and PPD signaling gene expression, which was inversely correlated with hydroxycoumarin and H2O2 accumulation. MNP Local A tubers exhibited longer storage life of 15 days with a low PPD score, higher metabolites synthesis, and gene expression. The PPD–resistant lines may be used to augment cassava breeding strategies for large–scale commercial and industrial use.
Thoungamba Amom, Leimapokpam Tikendra, Angamba Meetei Potshangbam, Nongthombam Bidyananda, Rajkumari Sanayaima Devi, Abhijit Dey, Manas Ranjan Sahoo, Wagner Aparecido Vendrame, Imlitoshi Jamir, and Potshangbam Nongdam
Elsevier BV
Mayengbam Premi Devi, Madhumita Dasgupta, Sansuta Mohanty, Susheel Kumar Sharma, Vivek Hegde, Subhra Saikat Roy, Rennya Renadevan, Kinathi Bipin Kumar, Hitendra Kumar Patel, and Manas Ranjan Sahoo
MDPI AG
Taro (Colocasia esculenta L. Schott, Araceae), an ancient root and tuber crop, is highly polygenic, polyphyletic, and polygeographic in nature, which leads to its rapid genetic erosion. To prevent the perceived loss of taro diversity, species discrimination and genetic conservation of promising taro genotypes need special attention. Reports on genetic discrimination of taro at its center of origin are still untapped. We performed DNA barcoding of twenty promising genotypes of taro indigenous to the northeastern hill region of India, deploying two chloroplast-plastid genes, matK and rbcL, and the ribosomal nuclear gene ITS2. The secondary structure of ITS2 was determined and molecular phylogeny was performed to assess genetic discrimination among the taro genotypes. The matK and rbcL genes were highly efficient (>90%) in amplification and sequencing. However, the ITS2 barcode region achieved significant discrimination among the tested taro genotypes. All the taro genotypes displayed most similar sequences at the conserved matK and rbcL loci. However, distinct sequence lengths were observed in the ITS2 barcode region, revealing accurate discriminations among the genotypes. Multiple barcode markers are unrelated to one another and change independently, providing different estimations of heritable traits and genetic lineages; thus, they are advantageous over a single locus in genetic discrimination studies. A dynamic programming algorithm that used base-pairing interactions within a single nucleic acid polymer or between two polymers transformed the secondary structures into the symbol code data to predict seven different minimum free energy secondary structures. Our analysis strengthens the potential of the ITS2 gene as a potent DNA barcode candidate in the prediction of a valuable secondary structure that would help in genetic discrimination between the genotypes while augmenting future breeding strategies in taro.
Leimapokpam Tikendra, Abhijit Dey, Imlitoshi Jamir, Manas Ranjan Sahoo, and Potshangbam Nongdam
Springer Science and Business Media LLC
Ingudam Bhupenchandra, Anjali Basumatary, Anil K. Choudhary, Adarsh Kumar, Dibyendu Sarkar, Sunil Kumar Chongtham, Athokpam Herojit Singh, Elangbam Lamalakshmi Devi, S. S. Bora, Menaka Devi Salam,et al.
Frontiers Media SA
Information on the role of boron (B) on soil physico-chemical and biological entities is scarce, and the precise mechanism in soil is still obscure. Present field investigation aimed to assessing the implication of direct and residual effect of graded levels of applied-B on soil biological entities and its concomitant impact on crop productivity. The treatments comprised of five graded levels of B with four replications. To assess the direct effect of B-fertilization, cauliflower was grown as a test crop wherein, B-fertilization was done every year. For assessment of succeeding residual effects of B-fertilization, cowpea and okra were grown as test crops and, B-fertilization was phased out in both crops. The 100% recommended dose of NPK (RDF) along with FYM was uniformly applied to all crops under CCOCS. Results indicated that the direct effect of B had the edge over residual effect of B in affecting soil physico-chemical and biological entities under CCOCS. Amongst the graded levels of B, application of the highest B level (2 kg ha–1) was most prominent in augmenting microbiological pools in soil at different crop growth stages. The order of B treatments in respect of MBC, MBN, and soil respiration at different crop growth stages was 2.0 kg B ha–1 > 1.5 kg B ha–1 > 1.0 kg B ha–1 > 0.5 kg B ha–1 > 0 kg B ha–1, respectively. Moreover, maximum recoveries of potentially mineralizable-C (PMC) and potentially mineralizable-N (PMN) were noticed under 2 kg B ha–1. Analogous trend was recorded in soil microbial populations at different crop growth stages. Similarly, escalating B levels up to 2 kg B ha–1 exhibited significantly greater soil enzymatic activities viz., arylsulphatase (AS), dehydrogenase (DH), fluorescein diacetate (FDA) and phosphomonoesterase (PMA), except urease enzyme (UE) which showed an antagonistic effect of applied-B in soil. Greater geometric mean enzyme activity (GMEA) and soil functional diversity index were recorded under 2 kg B ha–1 in CCOCS, at all crop growth stages over control. The inclusive results indicated that different soil physico-chemical and biological properties CCOCS can be invariably improved by the application of graded levels of B up to 2 kg B ha–1 in an acid Inceptisol.
Ingudam Bhupenchandra, Sunil Kumar Chongtham, Elangbam Lamalakshmi Devi, Ramesh R., Anil Kumar Choudhary, Menaka Devi Salam, Manas Ranjan Sahoo, Tshering Lhamu Bhutia, Soibam Helena Devi, Amarjit Singh Thounaojam,et al.
Frontiers Media SA
Climate change is a critical yield–limiting factor that has threatened the entire global crop production system in the present scenario. The use of biostimulants in agriculture has shown tremendous potential in combating climate change–induced stresses such as drought, salinity, temperature stress, etc. Biostimulants are organic compounds, microbes, or amalgamation of both that could regulate plant growth behavior through molecular alteration and physiological, biochemical, and anatomical modulations. Their nature is diverse due to the varying composition of bioactive compounds, and they function through various modes of action. To generate a successful biostimulatory action on crops under different parameters, a multi–omics approach would be beneficial to identify or predict its outcome comprehensively. The ‘omics’ approach has greatly helped us to understand the mode of action of biostimulants on plants at cellular levels. Biostimulants acting as a messenger in signal transduction resembling phytohormones and other chemical compounds and their cross–talk in various abiotic stresses help us design future crop management under changing climate, thus, sustaining food security with finite natural resources. This review article elucidates the strategic potential and prospects of biostimulants in mitigating the adverse impacts of harsh environmental conditions on plants.
Gobinda Chandra Acharya, Sansuta Mohanty, Madhumita Dasgupta, Supriya Sahu, Satyapriya Singh, Ayyagari V. V. Koundinya, Meenu Kumari, Ponnam Naresh, and Manas Ranjan Sahoo
MDPI AG
Commercial interest in the culinary herb, Eryngium foetidum L., has increased worldwide due to its typical pungency, similar to coriander or cilantro, with immense pharmaceutical components. The molecular delimitation and taxonomic classification of this lesser-known medicinal plant are restricted to conventional phenotyping and DNA-based marker evaluation, which hinders accurate identification, genetic conservation, and safe utilization. This study focused on species discrimination using DNA sequencing with chloroplast–plastid genes (matK, Kim matK, and rbcL) and the nuclear ITS2 gene in two Eryngium genotypes collected from the east coast region of India. The results revealed that matK discriminated between two genotypes, however, Kim matK, rbcL, and ITS2 identified these genotypes as E. foetidum. The ribosomal nuclear ITS2 region exhibited significant inter- and intra-specific divergence, depicted in the DNA barcodes and the secondary structures derived based on the minimum free energy. Although the efficiency of matK genes is better in species discrimination, ITS2 demonstrated polyphyletic phylogeny, and could be used as a reliable marker for genetic divergence studies understanding the mechanisms of RNA molecules. The results of this study provide insights into the scientific basis of species identification, genetic conservation, and safe utilization of this important medicinal plant species.
SATYAPRIYA SINGH, DEEPA SAMANT, MANAS RANJAN SAHOO, KUNDAN KISHORE, DINESH JINGER, and GOBINDA CHANDRA ACHARYA
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
In nutshell, invasive species causes serious threat to the natural ecosystem. Severe and up surging phenomenon of exotic rugose spiraling whitefly (RSW), Aleurodicus rugioperculatus (Martin) became a major concern in global crop production. The present study aimed to document and assesses the host range, nature and intensity of damage caused by this cryptic species. The roving survey was conducted to study the occurrence of RSW in the fruit orchards, vegetable gardens, ornamental crops and avenues trees plantation in the east-coast region of India. Hierarchical clustering was performed based on its incidence on different host plants and correlated with the dissimilarity index. The RSW was identified based on morphological characteristics and the nature of the damage. RSW infested a total of 27 crops. Four host plants, viz. Cocos nucifera (L.), Musa sp. Psidium guajava (L.) and Syzygium cumini (L.) (Skeels) showed severe levels of infestation. An important implication from the current study is the occurrence of its incidence on the two new host crops, viz. Amaranthus tricolor (L.) and Solanum torvum that was envisaged firstly to the scientific world. This study provides baseline information about RSW among various stakeholders, helps to develop a sustainable management strategy for timely mitigation of RSW menace.
Satyapriya Singh, Manas Ranjan Sahoo, Gobinda Chandra Acharya, Dinesh Jinger, and Priyanka Nayak
Springer Science and Business Media LLC
The agriculture production system is the gamble of biotic and abiotic stresses. However, biotic stresses pose more menace to the crops compared to abiotic stresses. Biotic stress especially arthropod pests are one of the major constraints in agricultural production globally. Silicone (Si) is recognized as a beneficial quasi mineral nutrient available most abundantly in the earth’s crust and has a robust effect on plant health by viably alleviating biotic and abiotic stresses. It strengthens plant resistance against arthropod pests. In this line, the present study fundamentally unites its attention on Si-mediated defense mechanism with physical, biochemical, or molecular traits, by boosting up host plant resistance against insect pests. Moreover, Si up-regulates phytohormone signaling pathways for example jasmonic acid and salicylic acid which induces the production of various herbivore induced plant volatiles (HIPVs) resulting in the strengthening of natural biological control by attracting more natural enemies. It further justifies the interactive signaling pathways and defensive enzyme production and their association in initiating resistance against biotic stresses. In addition, this study features the new research ponders and achievements that have effectively uncovered the essential part of Si in shielding the plants against herbivory pests. The article explores the potentiality of Si in up-regulating the resistance in plants against various economically significant crop pests and its importance to incorporation in sustainable pest management, further revealing insight upon future research issues in regards to the importance of Si in plant defense mechanisms against arthropod crop pests.
Gobinda Chandra Acharya, Naresh Ponnam, Meenu Kumari, Tapas Kumar Roy, Kodthalu Seetharamaiah Shivashankara, and Manas Ranjan Sahoo
Akademiai Kiado Zrt.
Abstract Spiny coriander (Eryngium foetidum L.) is a perennial medicinal herb grown in the tropical regions worldwide. In India, it is used as a potential spice for garnishing and flavoring the dishes and treating several ailments. Eryngium spp. found in coastal Odisha, India has a strong aroma similar to the seasonal Coriandrum. The volatile flavor constituents of the unique plants were analyzed through headspace solid-phase microextraction (HS-SPME) using capillary gas chromatography (GC) and gas chromatography-tandem mass spectrometry (GC–MS/MS). The volatile compounds exhibited high chemodiversity, with 10-undecenal as the major component in leaves (44.98%) and branches (57.43%). Fourier-transform infrared (FTIR) spectroscopy identified eight major peaks grouped into six main regions. Chemo profiles of these two corianders were overlapped and showed similar area differences in the spectral peak. The lesser-known perennial Eryngium with high chemodiversity would be a better alternative to the seasonal coriander for aromatic, pharmaceutical, and industrial uses.
Manas Ranjan Sahoo, Aparna Kuna, Mayengbam Premi Devi, Mandarapu Sowmya, and Madhumita Dasgupta
Springer Science and Business Media LLC
Nutrition-rich extruded snacks were developed from a mixture of cornflour, Bengal gram flour fortified by tree bean (TB) powder (0, 5, and 10%) using a twin-screw extruder. The nutritional, antioxidant, and amino acid profile and structural, functional, and sensory properties of the ready-to-eat (RTE) extruded snacks were evaluated. Ash, protein, and fiber content in TB-fortified extrudates were increased, whereas nitrogen-free extract was decreased. Total phenolics (2.34 mg g–1 FW), ascorbate (2.23 mg g–1 FW), total flavonoids (0.16 mg g–1 FW), and reduced glutathione (8.53 µM g–1 FW) were higher in the extrudates with 10% TB powder. Similarly, RTE extruded snacks fortified by 10% TB exhibited higher DPPH, FRAP, ABTS, hydroxyl radical activities, reducing power, and essential amino acids, such as lysine, leucine, isoleucine, cysteine, threonine, tyrosine, and methionine derived by HPLC. Extrudates fortified by 5% TB powder exhibited better microstructure through scanning electron microscopy. However, 10% TB powder possessed higher physicochemical properties and overall sensory attributes. This study reveals the tremendous industrial potential of nutrient-rich RTE extruded snacks fortified by underutilized TB (10%).
Facundo Pieniazek, Madhumita Dasgupta, Valeria Messina, Mayengbam Premi Devi, Yumnam Indrani Devi, Sansuta Mohanty, Satyapriya Singh, Bibhuti Bhusan Sahoo, Potshangbam Nongdam, Gobinda Chandra Acharya,et al.
MDPI AG
The localization of cuticular wax (CW) on the leaf epidermis and its interaction with the physiological mechanisms of three edible aroids, Alocasia, Colocasia, and Xanthosoma, were assessed. CW in the leaf tissues was visualized using scanning electron microscopy, which was higher in Colocasia (10.61 mg·dm−2) and Xanthosoma (11.36 mg·dm−2) than in Alocasia (1.36 mg·dm−2). Colocasia CW exhibited superhydrophobic properties with a higher static contact angle (CA) (>150°) than Xanthosoma (99.0°) and Alocasia (128.7°). The higher CW in Colocasia and Xanthosoma resulted in better leaf chlorophyll stability, moisture retention ability, and cellular membrane integrity compared to Alocasia. CW acted as a protecting barrier against deleterious solar radiation in terms of sun protection factor (SPF). The glossy appearance of wax crystals in the Alocasia leaf cuticles resulted in higher SPF. Overall, Colocasia CW highly influenced the qualitative and protective mechanisms of the leaf. Our study sheds light on the pivotal role of CW in the physiological properties of aroid leaves, which would be useful for the selection of wax-rich plants for augmenting future breeding strategies. The information would also be useful for further exploration of the industrial potential of superhydrophobic wax crystals obtained from edible aroids.
M.R. Sahoo, , K. Kishore, D.K. Dash, C.M. Panda, R.K. Panda, P.K. Nayak, , , ,et al.
Triveni Enterprises
Aim: Present study aimed to assess the efficacy of paclobutrazol on vegetative growth, root traits, antomical modifications and nutrient uptake in mango. Methodology: Pot experiment was conducted in young grafted plants of Amrapali variety treated with four concentrations of paclobutrazol viz., 100 mg l-1, 200 mg l-1, 500 mg l-1 and 1000 mg l-1. The design was completely randomized with four replications and each replicate comprised of fifteen plants. Data on different parameters were recorded in randomly selected plants and mean values were presented. Results: Plant height, leaf area, internodal length, plant biomass and shoot biomass reduced by 30.8%, 32.4%, 38.2%, 38.7% and 40.4%, respectively, at higher concentration of paclobutrazol (1000 mg l-1). However, there was an increase in leaf chlorophyll content with high PBZ concentrations (1000 mg l-1). Root traits like root biomass, growth rate and primary root reduced significantly at higher PBZ concentrations, whereas tertiary roots tended to increase. The size of xylem and phloem reduced substantially at higher PBZ concentration. First principal component (PC 1) explained more than 92% of the variance signifying most of the growth attributing traits. Interpretation: The results unveiled that growth attributing traits, vascular characteristics of root and stem, and leaf nutrient status were significantly influenced by higher concentration of PBZ.
Tongbram Roshni Devi, Paresh Chandra Kole, and Manas Ranjan Sahoo
Society for the Advancement of Horticulture
An efficient shoot and root organogenesis protocol has been developed from synseeds derived from various explants of Citrus jambhiri Lush. Optimum synseeds were developed using sodium alginate (0.5-0.75%) in 1.0% CaCl2 solution. Shoot organogenesis was examined under various basal vitamin mediums (MS Nitsch and MSB5) supplemented with various concentrations of adenine sulfate (ADS) and 6-benzyl amino purine (BAP) from different explants such as cotyledonary junction, shoot tip, and nodal explants. The synseed regeneration response ranged between 60-100% among the vitamins, cytokinins and explants used. The number of shoots per synseeds was higher (13.4) in MSB5-BAP (1.5 mg L-1), followed by 12.8 in MSN-ADS (2.0 mg L-1), 11.2 in MSN-ADS (1.0 mg L-1), and 10.8 in MSB5-ADS (1.0 mg L-1) from the synseeds developed using 0.75% sodium alginate. The mean number of roots per explant was higher (4.2) in ½MSN+IAA (1.0 mg L-1). Similarly, the mean root length was higher (5.2 cm) in ½MSN+IAA (0.5 mg L-1) followed by 4.2 cm in ½MSN+IAA (1.0 mg L-1). Regenerants derived from synseeds have shown no somaclonal variations, confirming that the plantlets are true-to-type to their parental progenies. The encapsulated plantlets showed >90% survivability while transferred at Kachai village, Manipur, India. The results of the present study encourage the use of various vitamin mediums and explants for large-scale propagation of C. jambhiri through synseeds.
Manas Ranjan Sahoo, Ponnam Naresh, Meenu Kumari, and Gobinda Chandra Acharya
Elsevier
Panda Bibhu Prasad, , Sahoo Manas Ranjan, and
Iraq Natural History Research Center and Museum
This note reported the first record of Spotted Flycatcher Muscicapa striata (Pallas, 1764) (Passeriformes, Muscicapidae) from the state of Odisha, India. This species was recorded from the north and western part of the country as well as from the Western Ghats, but this note reports the first record from the Eastern Ghats of India.
Satyapriya Singh, Biswajit Das, Anup Das, Sujan Majumder, Hidangmayum Lembisana Devi, Ranjeet Singh Godara, Alok Kumar Sahoo, and Manas Ranjan Sahoo
Springer Science and Business Media LLC
Abstract Background Traditional plant protection strategies have an integral part of food production system in North Eastern state Tripura, India, which has bestowed with rich heritage and biodiversity. However, there is no comprehensive report on the indigenous plant protection practices (IPPPs) specific to insect and vertebrate pest management, being followed by the inhabitants of the region for centuries. The present study was conducted to investigate, collect, and document the vulnerable IPPP practices followed by the native people from far flung locations of the Tripura. Methods The study aimed to document the IPPP following semi-structured questionnaires, participatory interaction, and direct observations with a total of 200 informants. We have calculated the relative frequencies of citation (RFC) for IPPP and estimated principal component analysis to link the status of IPPP with socio-demographic factors of the informants. The relationship between the field of IPPP used and different covariates (age, education, occupation, gender, location, and house type) was assessed using the Kruskal–Wallis test and Chi-square test. The relationship between adoption level and the respondents’ characteristics was analyzed using count regression analysis. Results The study found that the status of the IPPP has increased for mitigating pest issues. A total of 39 indigenous practices were recorded specifically to pest management from the ethnic people of Tripura, India. People acquired pretty knowledge about IPPP, and these were inherited from ancestors. The respondents in the study developed notable innovations for the management of many pest issues using locally available resources that warrant cost-effective and eco-friendly. Seed drying before storage to protect grain commodities was the most cited IPPP with a frequency of citation 0.675. In the field of IPPP used, the people primarily practiced agriculture + horticulture + storage category. An important implication from the study is the identification of two IPPP strategies in this region for the first time. Furthermore, the recorded IPPP used field was significantly associated with age, education, occupation, gender, locality, and house type. Likewise, the respondents’ socio-demographic variables were coupled considerably with the adoption of specific IPPP. Conclusion The reported IPPP for alleviating pest problems reflects the wisdom and generosity of the ethnic growers of Tripura, India. The study suggests the IPPP has strong potential in an integrated pest management approach passed down from generation to generation. The vulnerable practices largely remained unexplored due to inadequate scientific scrutiny and authenticity, yet in danger of being lost if not documented systematically. This study provides the first step toward accessing the valuable technology of untapped Tripura in IPPP and could be viable in paving action paradigm for their preservation, diffusion, and application with advanced pest management options.
Tongbram Roshni Devi, Madhumita Dasgupta, Manas Ranjan Sahoo, Paresh Chandra Kole, and Narendra Prakash
Public Library of Science (PLoS)
A protocol for high-frequency direct organogenesis from root explants of Kachai lemon (Citrus jambhiri Lush.) was developed. Full-length roots (~3 cm) were isolated from the in vitro grown seedlings and cultured on Murashige and Skoog basal medium supplemented with Nitsch vitamin (MSN) with different concentrations of cytokinin [6-benzylaminopurine, (BAP)] and gibberellic acid (GA3). The frequency of multiple shoot proliferation was very high, with an average of 34.3 shoots per root explant when inoculated on the MSN medium supplemented with BAP (1.0 mg L–1) and GA3 (1.0 mg L–1). Optimal rooting was induced in the plantlets under half strength MSN medium supplemented with indole-3-acetic acid (IAA, 0.5–1.0 mg L–1). IAA induced better root structure than 1-naphthaleneacetic acid (NAA), which was evident from the scanning electron microscopy (SEM). The expressions of growth regulating factor genes (GRF1 and GRF5) and GA3 signaling genes (GA2OX1 and KO1) were elevated in the regenerants obtained from MSN+BAP (1.0 mg L-1)+GA3 (1.0 mg L-1). The expressions of auxin regulating genes were high in roots obtained in ½ MSN+IAA 1.0 mg L-1. Furthermore, indexing of the regenerants confirmed that there was no amplicons detected for Huanglongbing bacterium and Citrus tristeza virus. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers detected no polymorphic bands amongst the regenerated plants. This is the first report that describes direct organogenesis from the root explant of Citrus jambhiri Lush. The high-frequency direct regeneration protocol in the present study provides an enormous significance in Citrus organogenesis, its commercial cultivation and genetic conservation.
Leimapokpam Tikendra, Angamba Meetei Potshangbam, Abhijit Dey, Tongbram Roshini Devi, Manas Ranjan Sahoo, and Potshangbam Nongdam
Springer Science and Business Media LLC
Dendrobium fimbriatum is an ornamental and medicinal orchid listed in the Red data book of IUCN. Phytohormones' effect on the in vitro regeneration of the orchid was studied using Mitra medium supplemented with different growth regulators. KN produced effective shoot formation when present alone or in combination with IBA or NAA. The shooting was gradually increased when KN concentration was increased from 0.8 to 4.8 mg L-1, but the opposite response was observed with BAP at higher concentration (4.8 mg L-1). IBA either in combination with BAP or KN promoted effective root development and multiplication. Micropropagated orchids grown in the basal medium devoid of any phytohormone showed 100% monomorphism, while low genetic polymorphism of 1.52% (RAPD-Random Amplification of Polymorphic DNA), 1.19% (ISSR-Inter Simple Sequence Repeat) and 3.97% (SCoT-Start Codon Targeted) was exhibited among the regenerants propagated in the hormone enriched medium. UPGMA (Unweighted pair group method using arithmetic averages) dendrograms showed the grouping of mother plant (MP) with the in vitro regenerants. The principal coordinate analysis (PCoA) further confirmed the clustering patterns as determined by the cluster analysis. The study reported for the first time the successful in vitro propagation of Dendrobium fimbriatum and their genetic stability assessment using molecular markers.