Agricultural and Biological Sciences, Plant Science, Plant Science, Agricultural and Biological Sciences
4
Scopus Publications
Scopus Publications
Efficacy of fungicides, bioagents and phytoextracts against Alternaria carthami of safflower in in vitro condition SS Wagh, AP Suryawanshi, DV Pawar Journal of Pure and Applied Microbiology, 2017 Safflower leaf spot / blight caused by Alternaria carthami (Chowdhury) is serious diseases in India. Considering the economic importance and seriousness of the disease, the present cost effective management practices against the test pathogen present in vitro studies were conducted during 2012-13. All the experiments were planned with CRD and all the treatments replicated thrice. A total six systemic fungicides @ 500, 1000 and 1500 ppm concentration and seven non-systemic / combi fungicides @ 1000, 2000 and 2500 ppm concentrations was evaluated in vitro against A. carthami, applying Poisoned food technique (Nene and Thapliyal, 1993). Results of the present studies revealed that systemic fungicides most effective than non-systemic against the test pathogen. Among systemic fungicides, average cent per cent inhibition of mycelial growth was observed in Hexaconazole (100 %), followed by Propiconazole (94.07 %) and Penconazole (94.75 %); Among non-systemic and combi- fungicides, significantly highest average mycelial growth inhibition was observed in Carbendazim 12 WP + Mancozeb 63 WP (85.80 %), followed by Mancozeb (82.59 %) and Copper-oxychloride (76.65 %). Among bioagents tested T. viride recorded significantly highest mycelial growth inhibition (87.04 %) of the test pathogen, followed by T. harzianum (82.59 %) and T. koningii (78.89 %). Among botanicals A. sativum was found most fungistatic and recorded significantly highest average mycelial growth inhibition 88.33 %, followed by A. indica (78.58 %), O. sanctum (73.83 %) and E. globulus (66.05 %).
RAPD based molecular diversity analysis of different Alternaria carthami isolates of safflower in Maharashtra SS Wagh, AP Suryawanshi, DV Pawar Journal of Pure and Applied Microbiology, 2017 Genetic diversity in safflower blight pathogen (Alternaria carthami) was analyzed using 20 isolates collected from major safflower growing regions of Maharashtra state in India. Experiment was conducted at Department of Agricultural Bioatechnology, College of Agriculture, Latur, VNMKV, Parbhani (M.S.). Genomic DNA extracted from each isolates of Alternaria carthami was subjected to polymerase chain reaction using 20 random decamer primers from OPA series. Only 08 of the 20 RAPD primers were selected based on repeatability. Result revealed that 100 per cent polymorphism were found. The dendrogram analysis revealed two clusters, Cluster I comprised twelve isolates viz., Ac-1, Ac-8, Ac-4, Ac-5, Ac-7, Ac-11, Ac-18, Ac-15, Ac-17, Ac-9, Ac-20 and Ac-19 with similarity coefficient of 77 per cent. Cluster II comprised of eight isolates viz., Ac-2, Ac-3, Ac-6, Ac-13, Ac-12, Ac-14, Ac-10 and Ac-16 with similarity coefficient of 76 per cent.
