BioMed. Sc. National Univ of Malaysia
MSc (Health Sc.) National Univ of Malaysia
Ph.D University of Stirling, UK
49
Scopus Publications
Scopus Publications
Length-Weight Relationship, Size Distribution and Sexual Maturity of Climbing Perch, Anabas testudineus (Bloch, 1792) From Different Habitats in Malaysia Mohamad Jalilah, Victor Tosin Okomoda, Khor Waiho, Hidayah Manan, Hassan Anuar, Thumronk Amornsakun, Musa Nadirah Tropical Life Sciences Research, 2026 The growth and reproductive performance of fish are influenced by ecological factors. This study investigated the biology of the climbing perch, Anabas testudineus, in relation to habitat. A total of 2,880 climbing perch were collected from three habitats (irrigation canal, swamp and paddy field) in Peninsular Malaysia during the dry and rainy seasons, and their biometric measurements (total length and body weight) and sexual maturity indices were measured. Two-way Classification Analysis (TCA) revealed significant sexual dimorphism in all morphometric parameters, significant habitat effects on fish size, and pronounced seasonal variation in environmental parameters, while condition factor and seasonal effects on morphometrics were not significant. Results showed that body size was largest in fish from irrigation canals during the rainy season, with males averaging 12.84 ± 1.17 cm in total length (32.38 ± 4.4 g body weight) and females averaging 13.70 ± 1.83 cm (52.84 ± 12.54 g), consistent with the upper size range reported for this species. Fish from irrigation canals also exhibited more advanced gonadal development, confirmed by higher gonadosomatic indices (GSI) and histological evidence of vitellogenic oocytes in females and spermatozoa-filled seminiferous lobules in males. Principal Component Analysis (PCA) indicated that larger body sizes and advanced gonadal development were associated with higher dissolved oxygen and pH, whereas smaller sizes correlated with elevated water temperature. This study highlights the critical roles of sex, habitat quality and seasonal variation in shaping the growth and reproductive traits of wild climbing perch populations and provides evidence-based insights for fisheries management in tropical freshwater ecosystems.
Analyze the toxicities of benzalkonium chloride as a COVID-19 disinfectant in physiological goldfish (Carassius auratus) Sisilia Rivanda Arianto, Fendi Aprian Syah, Luthfiana Aprilianita Sari, Ayu Lana Nafisyah, Sulastri Arsad, Nadirah Musa Veterinary World, 2023 Background and Aim: Coronavirus disease-2019 (COVID-19) impacts increasing the use of disinfectants (benzalkonium chloride), which indirectly accumulate in water. The disinfectant accumulation in waters has been studied, but there has been no study of its impact on aquatic commodities, especially fish with a high sensitivity, one of which is goldfish (Carassius auratus). Benzalkonium chloride can potentially affect several body proteins, including the cytoskeleton, nervous and endocrine systems, and fish physiology. This study aimed to determine the impact of benzalkonium chloride as a disinfectant on the level of color brightness, growth, gill histopathology, and mortality in goldfish. This investigation provides input into the impact of using disinfectants to prevent COVID-19 on aquatic commodities. Materials and Methods: This study utilized goldfish specimens sourced from Tulungagung, East Java, Indonesia. The experiment involved different concentration levels of benzalkonium chloride: (T1) 0 mg/L, (T2) 0.03 mg/L, (T3) 0.06 mg/L, (T4) 0.09 mg/L, and (T5) 0.12 mg/L. The research data were subjected to an analysis of variance for analysis. In cases where significant differences were observed, Duncan’s test was conducted for color brightness, growth, and mortality data. Furthermore, if the gill histopathological data yielded significant differences, additional tests were applied (Kruskal–Wallis and Mann–Whitney test). Results: The findings of this study demonstrated significant differences (p < 0.05) in the level of color brightness, growth, gill histopathology, and mortality in goldfish in response to varying concentrations of benzalkonium chloride. The relationship between the length and weight of the goldfish was analyzed using regression coefficients (b values), which were determined as 4.86, –0.04, –0.2, 0.8, and –0.07, respectively. Notably, the brightness level in the T2 group exhibited positive color results with a hue value of 11.55°, while optimal growth was observed in the T4 group, as evidenced by b value of 0.8. The gill histopathological data showed significant differences (p < 0.05). The scoring of histopathological damage in the goldfish gills ranged from 0 to 10, with higher scores indicating more severe damage. The highest total score of 10 was observed in the T5 group exposed to a concentration of 0.12 mg/L, resulting in an 85% mortality rate. This indicates that benzalkonium chloride, with its toxic compounds, can disrupt the respiratory system of fish and lead to death. Conclusion: The effects of benzalkonium chloride were evident even at a concentration of 0.03 mg/L. With increasing concentration, there was an increase in mortality rate, a decrease in growth, and a rise in histopathological damage to the gills. These findings highlight the negative impact of using conventional disinfectants on water and its organisms, emphasizing the need for further research on environmentally friendly alternatives. Keywords: color brightness, fisheries management, freshwater species, integrated multi-trophic aquaculture, mortality.
In vitro and in vivo characterisations of Centella asiatica extract against Vibrio alginolyticus infection in whiteleg shrimp, Penaeus vannamei Songklanakarin Journal of Science and Technology, 2023
Blood glucose level and infestation degree by Zeylanicobdella sp. in cantang grouper before and after closed wet transportation system G Mahasri, R M Khatami, A A Romadhon, N Musa, Kusnoto Iop Conference Series Earth and Environmental Science, 2023 The cantang grouper is one of the high-value fisheries commodities. Transportation is an integral activity in aquaculture, notably in transporting cantang grouper. Among the methods is the closed wet transportation system. The disrupted environment in the closed wet transportation system, exhaustion, and deficit of the immune system can cause the cantang grouper to be infested by marine leech or Zeylanicobdella. In this study, Zeylanicobdella sp. infestation levels and cantang grouper blood glucose levels are compared before and after a closed wet transportation system. This research used an experimental method. The 35 individuals’ sample was examined for blood glucose levels and the infestation degree of Zeylanicobdella. Subsequently, they were transported from Situbondo, East Java, to the Faculty of Fisheries and Marine Universitas Airlangga Surabaya and re-examined. The results of measuring blood glucose levels of cantang grouper were in the below-normal category, normal, and above-normal. There was no significant difference in the infestation degree by Zeylanicobdella sp. between before and after transportation. This study concludes that closed wet transportation affected blood glucose levels and did not affect the degree of infestation by Zeylanicobdella sp. in cantang grouper.
The Application of Microalgae Feeding Regime on Whiteleg Shrimp Culture in Each Stage: A Mini Review Sulastri Arsad, Rubina Melini Siallagan, Evellin Dewi Lusiana Evellin Dewi Lusiana, Muhammad Musa Muhammad Musa, Mohammad Mahmudi Mohammad Mahmudi, Luthfiana Aprilianita Sari Luthfiana Aprilianita Sari, Fiddy Semba Prasetiya Fiddy Semba Prasetiya, Nadirah Musa Sains Malaysiana, 2023 Feed management in shrimp culture aims to improve the quality of shrimp organisms. The application of the feeding regime in shrimp culture has been developed to determine the best formulation of diet in order to maximize growth rate and survival rate of shrimp by increasing feed efficiency and minimizing waste. The application of the feeding regime is closely related to the understanding of the physiological and morphological aspects of shrimp from the larval stage to the adult stage. The success of the application of feed management with the application of the feeding regime will be a solution to the problem of the high costs of feed needed in shrimp culture. The best formulation of diet in shrimp culture in the N6-P3 stage is microalgae, at the M1-PL10 stage is zooplankton, and in the juvenile-adult shrimp stage is artificial feed. Microalgae can significantly increase the survival rate and growth rate of shrimp at the hatchery stage. This article aimed to examine the effect of the application of feeding regime using different diet formulations to produce the highest growth performance of shrimp in shrimp culture based on the morphological and physiological aspects of shrimp.
The Effect of Different Additions of Carbon Dioxide on Growth, Lipids, Carotenoids and Chlorophyll-a of Chaetoceros calcitrans Esza Amaliandini, Syarif Nashrullah, Anastasya Larasati, Fajar Taufan, Luthfiana Sari, Nina Dewi, Sulastri Arsad, Nadirah Musa Journal of Ecological Engineering, 2023 Global warming gives phytoplankton a significant role in reducing carbon. C. calcitras is a phytoplankton which utilizes carbon dioxide for growth and the formation of secondary metabolites in order to survive. The purpose of this study was to determine the effect of differences in duration on growth, lipid content, carotenoids and chloro - phyll-a in C. calcitrans and to determine the optimal duration. An experimental method with statistical analysis using ANOVA was used in this study. The treatments implemented were the addition of carbon dioxide for 0, 2, 4, 6, 8, 10, 12, and 14 minutes with a volume of carbon dioxide addition of 3 bps. The results showed that the addition of carbon dioxide had a significant effect (P<0.05) on the value of lipids, growth, carotenoids and chlorophyll-a. The highest density value was obtained in Treatment for 2 minutes to increase growth with the highest total value of 2,927,500 cells/ml. The best treatment to increase lipids was Treatment for 12 minutes with lipid content of 63.33% and lipid productivity of 1.82 mg/l/day. The best treatment to increase carotenoid content was Treatment for 4 minutes with carotenoid content of 2.20 g/mL and chlorophyll-a content of 1.4431 g/mL. Optimal treatment differences to obtain the highest value of each parameter differ from each other, depending on the synthesis and physiological processes of C. calcitrans .
Escherichia coli POLLUTION IN COASTAL LAGOON AND DAM RREPERCUSSIONS ON PUBLIC HEALTH AND AQUACULTURE Najiah Musa, Ahmed Jalal Khan Chowdhury, Musa Nadirah, Kok Leong Lee, Nur Azna Saari, et al. Water Conservation and Management, 2023 Escherichia coli are rod-shaped gram-negative bacteria naturally present in the lower intestinal tracts of warm-blooded organisms including humans and are often released into the environment through faecal material and wastewater effluent. The presence of E. coli in waters has long been regarded as an indicator for recent faecal contamination, and possible presence of other more harmful enteropathogens. This study examined the total coliform load and occurrence of E. coli to determine the microbial water quality of Merchang Lagoon on Terengganu coast facing the South China Sea, and Raban Lake, part of the Chenderoh Hydroelectric Dam reservoir in the Lenggong Valley, Perak. Water samples were collected from three sites in the lagoon, and two sites in the lake. Total coliform load in water was enumerated by most probable number (MPN) method, and E. coli isolation was carried out using eosin methylene blue (EMB) agar. The results showed greatly varied total coliform counts from site to site in both the lagoon (site 1: 7; site 2: 220; site 3: 920 CFU/100 mL) and lake (site 1: 220; site 2: 1600 CFU/100 mL). All sites showed presence of E. coli. The results suggest faecal pollution, and the impact of water stagnation and human activities on the coliform load. The impacts of faecal pollution on public health and aquaculture in the investigated areas are discussed. Long-term monitoring of faecal pollution constitutes the essential part of environmental conservation and preservation against the risk of waterborne faecal pathogens in recreational and commercial activities.
White spot syndrome virus in wild mud crabs Sandra C. Zainathan, Nurshafiqah Norizan, Najiah Musa, Nadirah Musa, Faizah Shaharom-Harrison Aquaculture Pathophysiology Volume II Crustacean and Molluscan Diseases, 2022
Development of mud crab crablet, the identification of ciliates and the bioefficacy of leaf extract of Rhizophora apiculata as anti-protozoal agent Journal of Sustainability Science and Management, 2017
Health surveillance of freshwater prawn, Macrobrachium lanchesteri in Setiu Wetland, Terengganu, Malaysia Journal of Sustainability Science and Management, 2017
High water temperature impairs physiological responses in red hybrid tilapia: Effects on cortisol and its regulation Aacl Bioflux, 2017
In-vitro characterization of lytic bacteriophage PhVh6 as potential biocontrol agent against pathogenic Vibrio harveyi Aacl Bioflux, 2017
Some aspects of population biology of edible orange mud crab, Scylla olivacea (Herbst, 1796) during pre and post monsoon in Setiu Wetlands, Terengganu, Malaysia Journal of Sustainability Science and Management, 2017
In vitro evaluation of probiotic properties of Bacillus licheniformis strain UMTK003 from gut of wild orange mud crab, Scylla olivacea from Setiu Wetlands, Terengganu, Malaysia Journal of Sustainability Science and Management, 2017
Suitability of water salinity for hatching and survival of newly hatched larvae of climbing perch, Anabas testudineus Songklanakarin Journal of Science and Technology, 2014
Differential responses of Vibrio sp. To young and mature leaves extracts of Terminalia catappa L. International Food Research Journal, 2013
Antibiogram and plasmid profiling from Edwardsiella tarda isolated from freshwater fish in east coast Malaysia Journal of Sustainability Science and Management, 2011
