Suelen Adriani Marques

Scopus Publications

Treatment of spinal cord injury with biomaterials and stem cell therapy in non-human primates and humans
Ana Milena Silva Olaya, Fernanda Martins Almeida, Ana Maria Blanco Martinez, Suelen Adriani Marques
Neural Regeneration Research, 2025
Spinal cord injury results in the loss of sensory, motor, and autonomic functions, which almost always produces permanent physical disability. Thus, in the search for more effective treatments than those already applied for years, which are not entirely efficient, researches have been able to demonstrate the potential of biological strategies using biomaterials to tissue manufacturing through bioengineering and stem cell therapy as a neuroregenerative approach, seeking to promote neuronal recovery after spinal cord injury. Each of these strategies has been developed and meticulously evaluated in several animal models with the aim of analyzing the potential of interventions for neuronal repair and, consequently, boosting functional recovery. Although the majority of experimental research has been conducted in rodents, there is increasing recognition of the importance, and need, of evaluating the safety and efficacy of these interventions in non-human primates before moving to clinical trials involving therapies potentially promising in humans. This article is a literature review from databases (PubMed, Science Direct, Elsevier, Scielo, Redalyc, Cochrane, and NCBI) from 10 years ago to date, using keywords (spinal cord injury, cell therapy, non-human primates, humans, and bioengineering in spinal cord injury). From 110 retrieved articles, after two selection rounds based on inclusion and exclusion criteria, 21 articles were analyzed. Thus, this review arises from the need to recognize the experimental therapeutic advances applied in non-human primates and even humans, aimed at deepening these strategies and identifying the advantages and influence of the results on extrapolation for clinical applicability in humans.
A Novel Approach to Increase Glial Cell Populations in Brain Microphysiological Systems
Itzy E. Morales Pantoja, Lixuan Ding, Paulo E. C. Leite, Suelen A. Marques, July Carolina Romero, et al.
Advanced Biology, 2024
Brain microphysiological systems (bMPS) recapitulate human brain cellular architecture and functionality more closely than traditional monolayer cultures and have become increasingly relevant for the study of neurological function in health and disease. Existing 3D brain models vary in reflecting the relative populations of different cell types present in the human brain. Most models consist mainly of neurons, while glial cells represent a smaller portion of the cell populations. Here, by means of a chemically defined glial‐enriched medium (GEM), an improved method to expand the population of astrocytes and oligodendrocytes without compromising neuronal differentiation in bMPS, is presented. An important finding is that astrocytes also change in morphology when cultured in GEM, more closely recapitulating primary culture astrocytes. GEM bMPS are electro‐chemically active and show different patterns of calcium staining and flux. Synaptic vesicles and terminals observed by electron microscopy are also present. No significant changes in neuronal differentiation are observed by gene expression, however, GEM enhanced neurite outgrowth and cell migration, and differentially modulated neuronal maturation in two different cell lines. These results have the potential to significantly improve functionality of bMPS for the study of neurological diseases and drug discovery, contributing to the unmet need for safe human models.
NMDA receptor remodeling and nNOS activation in mice after unilateral striatal injury with 6-OHDA
Michele Barboza de Carvalho, Bruna Teixeira-Silva, Suelen Adriani Marques, Andrea Alice Silva, Marcelo Cossenza, et al.
Heliyon, 2024
Exercise Volume Can Modulate the Regenerative Response to Spinal Cord Injury in Mice
Anne Caroline Rodrigues dos Santos, Renata Pereira Laurindo, Fernanda Marques Pestana, Luiza dos Santos Heringer, Nathalie Henrique Silva Canedo, et al.
Neurotrauma Reports, 2024
Traumatic spinal cord injury (SCI) causes debilitating motor and sensory deficits that impair functional performance, and physical rehabilitation is currently the only established therapeutic reality in the clinical setting. In this study, we aimed to assess the effect of exercise of different volume and timing of intervention on functional recovery and neuromuscular regeneration in a mouse model of compressive SCI. Mice were assigned to one of four groups: laminectomy only (SHAM); injured, without treadmill training (SCI); injured, treadmill trained for 10 min until day 56 postinjury (TMT1); and injured, treadmill trained for two 10-min cycles with a 10-min pause between them until day 28 postinjury followed by the TMT1 protocol until day 56 postinjury (TMT3). On day 7 postinjury, animals started an eight-week treadmill-training exercise protocol and were trained three times a week. TMT3 mice had the best results in terms of neuroregeneration, functional recovery, and muscle plasticity as measured by functional and morphometric parameters. In conclusion, the volume of exercise can modulate the quality of the regenerative response to injury, when started in the acute phase and adjusted according to the inflammatory window.
Molecular approaches for spinal cord injury treatment
AnaMaria Blanco Martinez, FernandaMartins de Almeida, SuelenAdriani Marques, AnneCaroline Rodrigues dos Santos, CaioAndrade Prins, et al.
Neural Regeneration Research, 2023
Injuries to the spinal cord result in permanent disabilities that limit daily life activities. The main reasons for these poor outcomes are the limited regenerative capacity of central neurons and the inhibitory milieu that is established upon traumatic injuries. Despite decades of research, there is still no efficient treatment for spinal cord injury. Many strategies are tested in preclinical studies that focus on ameliorating the functional outcomes after spinal cord injury. Among these, molecular compounds are currently being used for neurological recovery, with promising results. These molecules target the axon collapsed growth cone, the inhibitory microenvironment, the survival of neurons and glial cells, and the re-establishment of lost connections. In this review we focused on molecules that are being used, either in preclinical or clinical studies, to treat spinal cord injuries, such as drugs, growth and neurotrophic factors, enzymes, and purines. The mechanisms of action of these molecules are discussed, considering traumatic spinal cord injury in rodents and humans.
Grafts of human adipose-derived stem cells into a biodegradable poly (acid lactic) conduit enhances sciatic nerve regeneration
Daniela F.P.A. Durço, Fernanda M. Pestana, Julia T. Oliveira, Bruna dos S. Ramalho, Lucas M. Souza, et al.
Brain Research, 2020
Novel CCM1 (KRIT1) Mutation Detection in Brazilian Familial Cerebral Cavernous Malformation: Different Genetic Variants in Inflammation, Oxidative Stress, and Drug Metabolism Genes Affect Disease Aggressiveness
Fabrícia Lima Fontes-Dantas, Gustavo da Fontoura Galvão, Elielson Veloso da Silva, Soniza Alves-Leon, Cláudia Cecília da Silva Rêgo, et al.
World Neurosurgery, 2020
Study of C─H⋯O Bond of Organic–Inorganic Hybrids Based on Polyhydroxybutyrate and Oxides Obtained Via an In Situ Sol–Gel Route
Anna Lecticia Martinez Martinez Toledo, Elton Jorge da Rocha Rodrigues, José Carlos Dutra Filho, Domethila Mariano de Souza Aguiar dos Santos, Suelen Adriani Marques, et al.
Polymer Engineering and Science, 2020
Sol–gel‐based zirconia (ZrO2) and silica (SiO2) were obtained in situ via hydrolysis–polycondensation of metal alkoxide precursors in membranes of poly(3‐hydroxybutyrate) (PHB). What followed was an assessment of thermal and spectroscopic effects from the insertion of the inorganic network in the PHB matrix. The findings exhibited a dependence of both the concentration and the chemical nature of the precursor on the properties of the as‐formed inorganic–organic hybrids. The results also indicated that positive nucleating effects, a reduction of the crystallite size and a variation in thermal transitions correlate with spectroscopic data from the alpha and beta crystal forms of the PHB matrix, as well as the crystallization data obtained through polarized light microscopy. POLYM. ENG. SCI., 60:673–681, 2020. © 2020 Society of Plastics Engineers
Mesenchymal stem cells and treadmill training enhance function and promote tissue preservation after spinal cord injury
Tamires Braga Massoto, Anne Caroline Rodrigues Santos, Bruna S. Ramalho, Fernanda Martins Almeida, Ana Maria Blanco Martinez, et al.
Brain Research, 2020
Intraaxial and Extraaxial Cavernous Malformation with Venous Linkage: Immune Cellular Inflammation Associated with Aggressiveness
Jorge Marcondes de Souza, Camilo Abud Aiex, Nathalie Henriques Silva Canedo, Suelen Adriani Marques, Ana Maria Blanco Martinez
World Neurosurgery, 2019
Lack of Galectin-3 attenuates neuroinflammation and protects the retina and optic nerve of diabetic mice
Henrique Rocha Mendonça, Juliana Nicolau Aranha Carvalho, Carla Andreia Abreu, Domethila Mariano de Souza Aguiar dos Santos, Julia Rios Carvalho, et al.
Brain Research, 2018
The diabetes drug liraglutide reverses cognitive impairment in mice and attenuates insulin receptor and synaptic pathology in a non-human primate model of Alzheimer's disease
Andre F Batista, Leticia Forny‐Germano, Julia R Clarke, Natalia M Lyra e Silva, Jordano Brito‐Moreira, et al.
Journal of Pathology, 2018
Blockade of ATP P2X7 receptor enhances ischiatic nerve regeneration in mice following a crush injury
Tatianne Ribeiro, Júlia Teixeira Oliveira, Fernanda Martins Almeida, Marcelo Amorim Tomaz, Paulo A. Melo, et al.
Brain Research, 2017
Chronic spinal cord lesions respond positively to tranplants of mesenchymal stem cells
Fernanda Martins de Almeida, Suelen Adriani Marques, Bruna dos Santos Ramalho, Tamires Braga Massoto, Ana Maria Blanco Martinez
Restorative Neurology and Neuroscience, 2015
A combination of schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration
Camila Oliveira Goulart, Sofia Jürgensen, Allana Souto, Júlia Teixeira Oliveira, Silmara de Lima, et al.
Plos One, 2014
Alzheimer’s disease-like pathology induced by amyloid-β oligomers in nonhuman primates
Leticia Forny-Germano, Natalia M. Lyra e Silva, André F. Batista, Jordano Brito-Moreira, Matthias Gralle, et al.
Journal of Neuroscience, 2014
A Highly Reproducible Mouse Model of Compression Spinal Cord Injury
Suelen Adriani Marques, Fernanda Martins de Almeida, Klauss Mostacada, Ana Maria Blanco Martinez
Methods in Molecular Biology, 2014
Comparison between suture and fibrin glue on repair by direct coaptation or tubulization of injured mouse sciatic nerve
Severina P. Félix, Fátima R. Pereira Lopes, Suelen A. Marques, Ana M.B. Martinez
Microsurgery, 2013
Human dental pulp cells: A new source of cell therapy in a mouse model of compressive spinal cord injury
Fernanda Martins de Almeida, Suelen Adriani Marques, Bruna dos Santos Ramalho, Rafaela Fintelman Rodrigues, Daniel Veloso Cadilhe, et al.
Journal of Neurotrauma, 2011
Transplantation of bone-marrow-derived cells into a nerve guide resulted in transdifferentiation into Schwann cells and effective regeneration of transected mouse sciatic nerve
Fátima Rosalina Pereira Lopes, Flávia Frattini, Suelen Adriani Marques, Fernanda Martins de Almeida, Lenira Camargo de Moura Campos, et al.
Micron, 2010
Predifferentiated embryonic stem cells promote functional recovery after spinal cord compressive injury
Suelen Adriani Marques, Fernanda Martins Almeida, Aline Marie Fernandes, Cleide dos Santos Souza, Daniel Veloso Cadilhe, et al.
Brain Research, 2010
Sciatic nerve regeneration is accelerated in galectin-3 knockout mice
Marcelo Sampaio Narciso, Bruno de Siqueira Mietto, Suelen Adriani Marques, Carolina Pontes Soares, Claudia dos Santos Mermelstein, et al.
Experimental Neurology, 2009
A simple, inexpensive and easily reproducible model of spinal cord injury in mice: Morphological and functional assessment
Suelen Adriani Marques, Valéria Ferreira Garcez, Elaine A. Del Bel, Ana Maria Blanco Martinez
Journal of Neuroscience Methods, 2009
Immunoelectron microscopy reveals the presence of neurofilament proteins in retinal terminals undergoing dark degeneration
Suelen Adriani Marques, Klauss Mostacada, Ana Maria Blanco Martinez
Brain Research, 2008
Diversity among satellite glial cells in dorsal root ganglia of the rat
R.S. Nascimento, M.F. Santiago, S.A. Marques, S. Allodi, A.M.B. Martinez
Brazilian Journal of Medical and Biological Research, 2008

RECENT SCHOLAR PUBLICATIONS

用生物材料和干细胞治疗非人类和人类灵长类动物的脊髓损伤
AMS Olaya, FM Almeida, AMB Martinez, SA Marques
中国神经再生研究 (英文版) 20 (2), 343 , 2025
2025
A novel approach to increase glial cell populations in brain microphysiological systems
IE Morales Pantoja, L Ding, PEC Leite, SA Marques, JC Romero, ...
Advanced biology 8 (8), 2300198 , 2024
2024
Citations: 13
Exercise Volume Can Modulate the Regenerative Response to Spinal Cord Injury in Mice
ACR Santos, RP Laurindo, FM Pestana, LS Heringer, NHS Canedo, ...
Neurotrauma Reports 5 (1), neur. 2024.0023 , 2024
2024
Citations: 2
NMDA receptor remodeling and nNOS activation in mice after unilateral striatal injury with 6-OHDA
MB de Carvalho, B Teixeira-Silva, SA Marques, AA Silva, M Cossenza, ...
Heliyon 10 (14) , 2024
2024
Citations: 4
Treatment of spinal cord injury with biomaterials and stem cell therapy in non-human primates and humans
SAM Ana Milena Silva Olaya, Fernanda Martins Almeida, Ana Maria Blanco Martinez
NEURAL REGENERATION RESEARCH 20 (2), 343-353 , 2024
2024
Citations: 9
脊髓损伤治疗的分子方法
FM de Almeida, SA Marques, ACR dos Santos, CA Prins, ...
中国神经再生研究 (英文版) 18 (1), 23 , 2023
2023
Molecular approaches for spinal cord injury treatment
FM de Almeida, SA Marques, ACR Dos Santos, CA Prins, ...
Neural regeneration research 18 (1), 23-30 , 2023
2023
Citations: 47
Altered excitatory and inhibitory neocortical circuitry leads to increased convulsive severity after pentylenetetrazol injection in an animal model of schizencephaly, but not …
L dos Santos Heringer, J Rios Carvalho, J Teixeira Oliveira, ...
Epilepsia open 7 (3), 462-473 , 2022
2022
Citations: 9
O ENSINO REMOTO DE DANÇA COMO INSTRUMENTO DE INCLUSÃO PARA ALUNOS COM NECESSIDADES ESPECIAIS
GM Dias, SA Marques
Cadernos de Docência e Inovação no Ensino Superior 1 (1) , 2022
2022
SALA DE AULA INVERTIDA E MAPAS MENTAIS COMO FERRAMENTAS DE CONSTRUÇÃO E CONSOLIDAÇÃO DO APRENDIZADO NO ENSINO REMOTO
MG Toledo, AC Paciello, SA Marques
Cadernos de Docência e Inovação no Ensino Superior 1 (1) , 2022
2022
Grafts of human adipose-derived stem cells into a biodegradable poly (acid lactic) conduit enhances sciatic nerve regeneration
DFPA Durço, FM Pestana, JT Oliveira, BS Ramalho, LM Souza, ...
Brain Research 1747, 147026 , 2020
2020
Citations: 9
Alzheimer's Disease-Like Pathology Induced by Amyloid-beta Oligomers in Nonhuman Primates (vol 34, pg 13629, 2014)
L Forny-Germano, NM Lyra e Silva, AF Batista, J Brito-Moreira, M Gralle, ...
JOURNAL OF NEUROSCIENCE 40 (42), 8204-8204 , 2020
2020
Citations: 1
Novel CCM1 (KRIT1) mutation detection in Brazilian familial cerebral cavernous malformation: different genetic variants in inflammation, oxidative stress, and drug metabolism …
FL Fontes-Dantas, G da Fontoura Galvão, EV da Silva, S Alves-Leon, ...
World Neurosurgery 138, 535-540. e8 , 2020
2020
Citations: 13
Study of C─H⋯O Bond of Organic–Inorganic Hybrids Based on Polyhydroxybutyrate and Oxides Obtained Via an In Situ Sol–Gel Route
AL Martinez Martinez Toledo, EJ da Rocha Rodrigues, JC Dutra Filho, ...
Polymer Engineering & Science 60 (4), 673-681 , 2020
2020
Citations: 1
Mesenchymal stem cells and treadmill training enhance function and promote tissue preservation after spinal cord injury
TB Massoto, ACR Santos, BS Ramalho, FM Almeida, AMB Martinez, ...
Brain Research 1726, 146494 , 2020
2020
Citations: 38
Intraaxial and extraaxial cavernous malformation with venous linkage: immune cellular inflammation associated with aggressiveness
JM de Souza, CA Aiex, NHS Canedo, SA Marques, AMB Martinez
World Neurosurgery 131, 87-89 , 2019
2019
Citations: 5
Lack of Galectin-3 attenuates neuroinflammation and protects the retina and optic nerve of diabetic mice
HR Mendonça, JNA Carvalho, CA Abreu, JR Carvalho, SA Marques, ...
Brain research 1700, 126-137 , 2018
2018
Citations: 48
The diabetes drug liraglutide reverses cognitive impairment in mice and attenuates insulin receptor and synaptic pathology in a non‐human primate model of Alzheimer's disease
AF Batista, L Forny‐Germano, JR Clarke, NM Lyra e Silva, J Brito‐Moreira, ...
The Journal of pathology 245 (1), 85-100 , 2018
2018
Citations: 263
Blockade of ATP P2X7 receptor enhances ischiatic nerve regeneration in mice following a crush injury
T Ribeiro, JT Oliveira, FM Almeida, MA Tomaz, PA Melo, SA Marques, ...
Brain Research 1669, 69-78 , 2017
2017
Citations: 13
Chronic spinal cord lesions respond positively to tranplants of mesenchymal stem cells
FM de Almeida, SA Marques, BS Ramalho, TB Massoto, AMB Martinez
Restorative neurology and neuroscience 33 (1), 43-55 , 2015
2015
Citations: 31

MOST CITED SCHOLAR PUBLICATIONS

Alzheimer's disease-like pathology induced by amyloid-β oligomers in nonhuman primates
L Forny-Germano, NM Lyra e Silva, AF Batista, J Brito-Moreira, M Gralle, ...
The Journal of Neuroscience 34 (41), 13629-13643 , 2014
2014
Citations: 265
The diabetes drug liraglutide reverses cognitive impairment in mice and attenuates insulin receptor and synaptic pathology in a non‐human primate model of Alzheimer's disease
AF Batista, L Forny‐Germano, JR Clarke, NM Lyra e Silva, J Brito‐Moreira, ...
The Journal of pathology 245 (1), 85-100 , 2018
2018
Citations: 263
Human dental pulp cells: a new source of cell therapy in a mouse model of compressive spinal cord injury
FM de Almeida, SA Marques, BS Ramalho, RF Rodrigues, DV Cadilhe, ...
Journal of neurotrauma 28 (9), 1939-1949 , 2011
2011
Citations: 119
A simple, inexpensive and easily reproducible model of spinal cord injury in mice: morphological and functional assessment
SA Marques, VF Garcez, EA Del Bel, AMB Martinez
Journal of neuroscience methods 177 (1), 183-193 , 2009
2009
Citations: 118
Comparison between suture and fibrin glue on repair by direct coaptation or tubulization of injured mouse sciatic nerve
SP Félix, FR Pereira Lopes, SA Marques, AMB Martinez
Microsurgery 33 (6), 468-477 , 2013
2013
Citations: 68
Diversity among satellite glial cells in dorsal root ganglia of the rat
RS Nascimento, MF Santiago, SA Marques, S Allodi, AMB Martinez
Brazilian Journal of Medical and Biological Research 41 (11), 1011-1017 , 2008
2008
Citations: 62
Sciatic nerve regeneration is accelerated in galectin-3 knockout mice
MS Narciso, B de Siqueira Mietto, SA Marques, CP Soares, ...
Experimental Neurology 217 (1), 7-15 , 2009
2009
Citations: 59
Predifferentiated embryonic stem cells promote functional recovery after spinal cord compressive injury
SA Marques, FM Almeida, AM Fernandes, C dos Santos Souza, ...
Brain research 1349, 115-128 , 2010
2010
Citations: 55
Transplantation of bone-marrow-derived cells into a nerve guide resulted in transdifferentiation into Schwann cells and effective regeneration of transected mouse sciatic nerve
FRP Lopes, F Frattini, SA Marques, FM de Almeida, ...
Micron 41 (7), 783-790 , 2010
2010
Citations: 52
A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration
CO Goulart, S Jürgensen, A Souto, JT Oliveira, S de Lima, C Tonda-Turo, ...
PloS one 9 (10), e110090 , 2014
2014
Citations: 51
Lack of Galectin-3 attenuates neuroinflammation and protects the retina and optic nerve of diabetic mice
HR Mendonça, JNA Carvalho, CA Abreu, JR Carvalho, SA Marques, ...
Brain research 1700, 126-137 , 2018
2018
Citations: 48
Molecular approaches for spinal cord injury treatment
FM de Almeida, SA Marques, ACR Dos Santos, CA Prins, ...
Neural regeneration research 18 (1), 23-30 , 2023
2023
Citations: 47
Mesenchymal stem cells and treadmill training enhance function and promote tissue preservation after spinal cord injury
TB Massoto, ACR Santos, BS Ramalho, FM Almeida, AMB Martinez, ...
Brain Research 1726, 146494 , 2020
2020
Citations: 38
Chronic spinal cord lesions respond positively to tranplants of mesenchymal stem cells
FM de Almeida, SA Marques, BS Ramalho, TB Massoto, AMB Martinez
Restorative neurology and neuroscience 33 (1), 43-55 , 2015
2015
Citations: 31
Participation of neurofilament proteins in axonal dark degeneration of rat’s optic nerves
SA Marques, M Taffarel, AMB Martinez
Brain research 969 (1-2), 1-13 , 2003
2003
Citations: 30
A highly reproducible mouse model of compression spinal cord injury
SA Marques, FM de Almeida, K Mostacada, AMB Martinez
Axon Growth and Regeneration: Methods and Protocols, 149-156 , 2014
2014
Citations: 14
A novel approach to increase glial cell populations in brain microphysiological systems
IE Morales Pantoja, L Ding, PEC Leite, SA Marques, JC Romero, ...
Advanced biology 8 (8), 2300198 , 2024
2024
Citations: 13
Novel CCM1 (KRIT1) mutation detection in Brazilian familial cerebral cavernous malformation: different genetic variants in inflammation, oxidative stress, and drug metabolism …
FL Fontes-Dantas, G da Fontoura Galvão, EV da Silva, S Alves-Leon, ...
World Neurosurgery 138, 535-540. e8 , 2020
2020
Citations: 13
Blockade of ATP P2X7 receptor enhances ischiatic nerve regeneration in mice following a crush injury
T Ribeiro, JT Oliveira, FM Almeida, MA Tomaz, PA Melo, SA Marques, ...
Brain Research 1669, 69-78 , 2017
2017
Citations: 13
Treatment of spinal cord injury with biomaterials and stem cell therapy in non-human primates and humans
SAM Ana Milena Silva Olaya, Fernanda Martins Almeida, Ana Maria Blanco Martinez
NEURAL REGENERATION RESEARCH 20 (2), 343-353 , 2024
2024
Citations: 9

Suelen Adriani Marques

RESEARCH, TEACHING, or OTHER INTERESTS

Scopus Publications

RECENT SCHOLAR PUBLICATIONS

MOST CITED SCHOLAR PUBLICATIONS