Bernardo Garziera Gasperin
@ufpel.edu.br
Faculdade de Veterinária
UNIVERSIDADE FEDERAL DE PELOTAS
RESEARCH INTERESTS
Tem experiência na área de Medicina Veterinária, com ênfase em fisiologia, ginecologia, andrologia e biotécnicas da reprodução animal, atuando principalmente nos seguintes temas: Biotécnicas da reprodução como inseminação artificial, superovulação e transferência de embriões e produção de embriões i
Scopus Publications
Scopus Publications
Jéssica Lazzari, José Victor Vieira Isola, Vanessa Lambrecht Szambelan, Gabriel Menegazzi, Marcos Busanello, Monique Tomazele Rovani, Juliana Sarubbi, Eduardo Schmitt, Rogerio Ferreira, Paulo Bayard Dias Gonçalves,et al.
Elsevier BV
Raquel Grande Pereira, Otávio Saraiva Pires, Clério Antônio Hoefle, Alexandro Fritzen, Giovanna Fiordalisi, Juliana Germano Ferst, Fernando Caetano de Oliveira, Arnaldo Diniz Vieira, Rafael Gianella Mondadori, Gabriel Maggi,et al.
Elsevier BV
José Victor Vieira Isola, Henrique Schneider Pestano, Sergio Farias Vargas Junior, Fernando Caetano de Oliveira, Fabiane Pereira de Moraes, Camila Ribeiro Carvalho de Brito, Lucas Lourenço Castiglioni Guidoni, Rogério Ferreira, Arnaldo Diniz Vieira, Monique Tomazele Rovani,et al.
FapUNIFESP (SciELO)
ABSTRACT: Protocols to artificially induce cows and heifers into lactation are effective and commercially available in Brazil. However, these protocols demand long hormonal treatments, which is debatable since little research has been done in the subject. To further understand artificial induction to lactation (AIL) protocols, we conducted two experiments. In experiment 1, our objective was to better characterize steroidal profile during an AIL protocol and assess the estradiol (E2) and progesterone (P4) serum profiles of Jersey cows (n=6) subjected to a conventional protocol. In experiment 2, we aimed to compare milk production and serum E2 concentrations of Holstein heifers induced into lactation by the standard AIL protocol (15 injections of E2 and 8 P4 injections) and by a short protocol in which the number of E2 injections and, consequently, the overall dosage of E2, were reduced (8 injections of E2 and 8 P4 injections). We hypothesized that a short AIL protocol (8 days) would be as efficient as the long standard protocol of 15 days. Our hypothesis was confirmed, since we demonstrated that a shorter protocol was able to induce lactation in Holstein heifers without hindering milk production.
Camila R.C. Brito, Ágatha D. Cordeiro, Pricila Baldessar, Carolini Schultz, Monike Quirino, Rafael R. Ulguim, Paulo B.D. Gonçalves, Thomaz Lucia, Ivan Bianchi, and Bernardo G. Gasperin
Elsevier BV
Daniele Missio, Fabio Gallas Leivas, Francielli Cibin, Tatiana Emanuelli, Sabrina Somacal, Vanessa Buss, Bernardo Gasperin, Dimas Estrasulas de Oliveira, Paulo Bayard Dias Gonçalves, and Rogério Ferreira
Wiley
AbstractIn the postpartum period, there is an increase in non‐esterified fatty acids (NEFA) in both serum and follicular fluid (FF) of cattle. The increase in fatty acid concentration results in increased production of reactive oxygen species (ROS) that can compromise bovine fertility. The objectives of this study were to characterize the lipid profile found in the FF of cows experiencing induced negative energy balance (NEB) and to evaluate the effect of α‐tocopherol in the prevention of oxidative stress in the serum and FF of cows. Twenty‐nine beef cows were divided into groups: (1) control; (2) Fasting for 24 days; and (3) Fasting + VitE. Between D0 and D4 blood samples were taken to assess concentrations of NEFA, ROS production, total antioxidant capacity (FRAP), lipid peroxidation, and α‐tocopherol (vitamin E). On D4, follicular aspiration was performed for analysis of FF from the dominant follicle. Our results demonstrate that fasting was effective in causing increased fat mobilization in animals. The increase in serum concentration of C18:1c9 was reflected in the FF of fasting cows. Serum α‐tocopherol concentration was higher in the control and Fasting + VitE groups compared to the Fasting group. In FF, there was an increase of α‐tocopherol in the Fasting + VitE group in comparison to Fasting cows. There was an increase in ROS production in the serum of fasting cows. ROS production in FF was higher in the Fasting compared to the Fasting + VitE group. Vitamin E has beneficial effects in reducing ROS production in the dominant follicle of cows in NEB.
Samira A. Silva, Rafael G. Mondadori, Gabrielly S. Noleto, Ingrid P. Barbosa, Reuel L. Gonçalves, Bernardo G. Gasperin, Monique T. Rovani, Eanes F. Paz, Leonardo S. Gomes, and Luiz F.M. Pfeifer
Elsevier BV
J. Isola, G. B. Veiga, Camila R C de Brito, J. A. Alvarado-Rincón, D. N. Garcia, B. Zanini, Jéssica D Hense, A. D. Vieira, M. Garratt, B. Gasperin,et al.
P. Baldessar, C.R.C. de Brito, H. Johann, C. Schultz, V. Peripolli, F. Moreira, T. Lucia, R.R. Ulguim, B.G. Gasperin, and I. Bianchi
Elsevier BV
Ágatha Decroix Cordeiro, Ana Júlia B. Conterato, Ivan Bianchi, Débora L. A. Dartora, Ricardo Zanella, Carlos Bondan, Mariana G. Marques, Bernardo G. Gasperin, and Thomaz Lucia
Wiley
A protocol to induce lactation was applied to non-pregnant gilts. In Experiment I, five gilts with estrus synchronized through oral supplementation of 20 mg Altrenogest for 18 d received: 10 mg estradiol cypionate (EC) on the last day of estrus expression (D0); 10 mg EC and 300 mg long-acting progesterone (P4) on D26; and two 0.53 mg doses of a prostaglandin F2α analogue (PGF) 12 h apart on D36. Blood was collected on D12, D19, D26 and D33. Milk secretion started in all gilts 24 h after PGF administration and lasted at least 8 d. Milk samples were collected from D37 to D45. The serum P4 concentration was lower on D12 than subsequently (P < 0.05), but the estradiol concentration was unaltered (P > 0.05). The milk produced during the induced lactation was generally richer in protein and poorer in fat compared to the milk from the lactation of a reference sow. In Experiment II, the same protocol induced lactation in two gilts, which nursed fostered piglets for 22 d. Thus, lactation was induced in all treated gilts and the milk produced was capable to nurture fostered piglets.
Natalia Machado Rahal, Gabriela Bueno Luz, Kauê Rodriguez Martins, Bernardo Garziera Gasperin, Josiane de Oliveira Feijó, André Gustavo Cabrera Dalto, Monique Tomazele Rovani, Rodrigo Casquero Cunha, and Marcio Nunes Corrêa
FapUNIFESP (SciELO)
Abstract The aim of this study was to investigate the association between chronic Anaplasma marginale and Babesia spp. infection and hematological parameters of pregnant and non-pregnant taurine heifers. Blood samples from 94 females were collected on the first day (D-10) of timed artificial insemination (TAI) protocol and on pregnancy diagnosis (D+34). Hematological parameters were determined and compared between pregnant (PG) and non-pregnant (NPG) heifers, and within group at different sampling days. Real-time PCR (qPCR) was used to determine A. marginale and Babesia bovis infection, and for absolute quantification of Babesia spp. between PG and NPG groups. Correlation analysis was performed between the number of gDNA copies (CN) of Babesia spp. and hematological parameters. On D-10, mean hemoglobin concentration was higher for NPG, and hematocrit and total plasma protein were higher on D+34 for both groups. There was no difference in Babesia spp. CN between groups. In the first qPCR, all heifers were positive for A. marginale and B. bovis. Significant correlations were found between hemoglobin and erythrocyte and between hemoglobin and hematocrit (r = 0.8082 and r = 0.3009, respectively). Low levels of A. marginale and Babesia spp. did not affect hematological parameters of chronically infected pregnant and non-pregnant taurine heifers.
Bruno Tomazele Rovani, Vitor Braga Rissi, Monique Tomazele Rovani, Bernardo Garziera Gasperin, Tadeu Baumhardt, Vilceu Bordignon, Liliane de Freitas Bauermann, Daniele Missio, and Paulo Bayard Dias Gonçalves
FapUNIFESP (SciELO)
Abstract Radiotherapy causes destruction of tumor cells, but also threatens the integrity and survival of surrounding normal cells. Then, woman submitted to irradiation for cancer treatment may present permanent ovary damage, resulting in impaired fertility. The objective of this study was to investigate the effects of therapeutic doses of ionizing radiation (IR), used for ovarian cancer treatment in humans, on bovine cumulus-oocyte complexes (COCs) as experimental model. Bovine ovaries were exposed to 0.9 Gy, 1.8 Gy, 3.6 Gy or 18.6 Gy IR, and then COCs were collected and used to evaluate: (a) oocyte nuclear maturation; (b) presence of phosphorylated H2A.X (γH2AX), as an indicator of DNA double-strand breaks (DSBs); and (c) expression of genes involved in DNA repair (TP53BP1, RAD52, ATM, XRCC6 and XRCC5) and apoptosis (BAX). The radiation doses tested in this study had no detrimental effects on nuclear maturation and did not increase γH2AX in the oocytes. However, IR treatment altered the mRNA abundance of RAD52 (RAD52 homolog, DNA repair protein) and BAX (BCL2-associated X protein). We conclude that although IR doses had no apparent effect on oocyte nuclear maturation and DNA damage, molecular pathways involved in DNA repair and apoptosis were affected by IR exposure in cumulus cells.
A.S. Maffi, J.A. Alvarado. Rincón, A.A. Barbosa, M.A. Weiller, R.G. Mondadori, B.G. Gasperin, M.T. Rovani, L.A.X. Cruz, K.C. Freitas, A. Schneider,et al.
FapUNIFESP (SciELO)
ABSTRACT The objective of the present study was to evaluate the effect of lipopolysaccharide (LPS) administration on activation and apoptosis of primordial follicles. There was no difference in the total number of follicles as well as in the different types of follicles. Furthermore, the LPS challenge didn’t modulate the expression of genes related with ovarian reserve (HAM), oocyte survival (Survivin), activation rate (Pten, KIT, KITL1, KITL2, AKT1, SIRT1), and follicular abnormalities. Therefore, the LPS exposure with 24h interval had no effect on activation rate and primordial follicles abnormalities, and also had no effect on expression of anti-apoptotic genes and genes related with ovarian reserve, oocyte survival, activation rate, and primordial follicles abnormalities.
Monique Mazzarollo Frata, Wagner Marques de Lima, Danylo Cintra Medeiros Lima, Dênis Halinski da Silveira, Thomaz Lucia Júnior, Rogério Ferreira, Paulo Bayard Dias Gonçalves, and Bernardo Garziera Gasperin
FapUNIFESP (SciELO)
ABSTRACT: Treatment with prostaglandin F2α (PGF) induces ovulation and increases conception rates in cows, while improving embryo production in buffalos. However, its effect on superovulated cows is unknown. This study verified whether single PGF administration concurrent with artificial insemination (AI) improves fertilization and embryo production rates in superovulated cows. In each replicate, embryo donor cows were equally allocated to two groups: the untreated control and PGF groups. The latter of which received 482 µg of cloprostenol concurrent with the first AI. Each cow (n = 35) was subjected to two superovulations (SOV) in a crossover design (total = 70 embryo collections). In the control and PGF groups, respectively, the observed responses were [median (95% CI)]: 12 (10-18) and 15 (12-18) total structures, 9 (7-11) and 7 (6-10) viable embryos, 1 (0-1) and 1 (1-3) degenerated embryos, and 1 (0-3) and 2 (0-5) oocytes (P > 0.05). In conclusion, single PGF treatment concurrent with the first AI did not affect embryo production in superovulated cows.
Camila Amaral D'Avila, Fabiane Pereira de Moraes, Andrez Pastorello Bohn, Monique Tomazele Rovani, Arnaldo Diniz Vieira, Rogério Ferreira, José Nélio de Sousa Sales, Hernan Baldassarre, Rafael Gianella Mondadori, Paulo Bayard Dias Gonçalves,et al.
Elsevier BV
L.F.M. Pfeifer, B.G. Gasperin, J.P. Cestaro, and A. Schneider
Elsevier BV
Andressa Minussi Pereira Dau, Paulo Roberto da Rosa, Joabel dos Santos, Juliana Ferst, Mariana de Macedo, Monique Rovani, Fabio Comim, Alfredo Quites Antoniazzi, Bernardo Gasperin, Rogério Ferreira,et al.
Elsevier BV
Cristina S. Haas, Fernando C. Oliveira, Monique T. Rovani, Juliana G. Ferst, Sergio Farias Vargas Jr, Arnaldo D. Vieira, Rafael Gianella Mondadori, Lígia M.C. Pegoraro, Paulo B.D. Gonçalves, Vilceu Bordignon,et al.
Elsevier BV
José V.V. Isola, Bianka M. Zanini, Jessica D. Hense, Joao A. Alvarado-Rincón, Driele N. Garcia, Giulia C. Pereira, Arnaldo D. Vieira, Thais L. Oliveira, Tiago Collares, Bernardo G. Gasperin,et al.
Elsevier BV
Calorie restriction (CR) (25-40%) is the most commonly studied strategy for curtailing age-related disease and has also been found to extend reproductive lifespan in female mice. However, the effects of mild CR (10%), which is sustainable, on ovarian aging has not yet been addressed. 17α-estradiol (17α-E2) is another intervention shown to positively modulate healthspan and lifespan in mice but its effects on female reproduction remain unclear. We evaluated the effects of mild CR (10%) and 17α-E2 treatment on ovarian reserve and female fertility over a 24-week period, and compared these effects with the more commonly employed 30% CR regimen. Both 10% and 30% CR elicited positive effects on the preservation of ovarian reserve, whereas 17α-E2 did not alter parameters associated with ovarian function. Following refeeding, both 10% and 30% increased fertility as evidenced by greater pregnancy rates. In aligned with the ovarian reserve data, 17α-E2 also failed to improve fertility. Collectively, these data indicate that 10% CR is effective in preserving ovarian function and fertility, while 17α-E2 does not appear to have therapeutic potential for delaying ovarian aging.
Gabriella dos Santos Velho, Monique Tomazele Rovani, Rogério Ferreira, Bernardo Garziera Gasperin, and André Gustavo Cabrera Dalto
Wiley
A feasible and non-invasive luteal function assessment which enables timely intervention to improve progesterone (P4) support at early pregnancy is not well-established. The aim of this study was to determine the correlation among morphological and functional assessment methods of corpus luteum (CL) on Day 5 (D5) following timed-artificial insemination effect on luteal blood perfusion (LBP), CL diameter, and serum P4 concentration. Beef heifers (n = 89) were synchronized and inseminated (D0). On D5, CL was scanned by color-Doppler ultrasonography using a LBP score (0 = absent; 3 = high blood perfusion); CL diameter was obtained; and blood was collected. Diameter of CL had a positive linear effect on P4 concentration (P <0.001); and larger CL diameter increased the probability to become pregnant (P <0.05; odds ratio = 1.21). Heifers with a CL larger than 14.95 mm had higher pregnancy rate than heifers with a 14.95 mm or smaller CL (P <0.05). Animals with LBP 2 and 3 had larger CL when compared to scores 0 and 1 (P <0.001). Scores 1, 2 and 3 can accurately estimate heifers with higher P4 (accuracy = 0.79, 0.72 and 0.61, respectively). In conclusion, LBP on Day 5 post-TAI allows to estimate heifers with higher P4 and larger CL size, and a larger CL diameter was positively associated with pregnancy rate.
Júlia Koch, Valério Marques Portela, Esdras Corrêa Dos Santos, Daniele Missio, Leonardo Guedes de Andrade, Zigomar da Silva, Bernardo Garziera Gasperin, Alfredo Quites Antoniazzi, Paulo Bayard Dias Gonçalves, and Gustavo Zamberlam
Springer Science and Business Media LLC
To determine if the inhibition of the interaction between the Hippo effector YAP or its transcriptional co-activator TAZ with the TEAD family of transcription factors is critical for the cumulus expansion–related events induced by the EGF network in cumulus-oocyte complexes (COCs). We performed a series of experiments using immature bovine COCs subjected to an IVM protocol for up 24 h in which cumulus expansion was stimulated with EGF recombinant protein or FSH. The main results indicated that EGFR activity stimulation in bovine cumulus cells (CC) increases mRNA levels encoding the classic YAP/TAZ-TEAD target gene CTGF. To determine if important genes for cumulus expansion are transcriptional targets of YAP/TAZ-TEAD interaction in CC, COCs were then subjected to IVM in the presence of FSH with or without distinct concentrations of Verteporfin (VP; a small molecule inhibitor that interferes with YAP/TAZ binding to TEADs). COCs were then collected at 6, 12, 18, and 24 h for total RNA extraction and RT-qPCR analyses. This experiment indicated that VP inhibits in a time- and concentration-dependent manner distinct cumulus expansion and oocyte maturation–related genes, by regulating EGFR and CTGF expression in CC. Taken together, the results presented herein represent considerable insight into the functional relevance of a completely novel signaling pathway underlying cumulus expansion and oocyte maturation in monovulatory species. YAP/TAZ or CTGF may represent potential targets to improve the efficiency of IVM systems, not only for monovulatory species of agricultural importance as the cow, but for human embryo production.
Ingrid P. Barbosa, Jamyle P. Cestaro, Samira A. Silva, Gabrielly S. Noleto, Reuel L. Gonçalves, George M. Silva, Felipe H. Paes, Bernardo G. Gasperin, Monique T. Rovani, and Luiz F.M. Pfeifer
Elsevier BV
Two experiments evaluated the effects of gonadotropin releasing hormone (GnRH) treatment on fertility of suckled Nelore beef cows treated with an estradiol/progesterone (P4)-based protocol for timed artificial insemination (TAI). Experiment 1 was designed to determine the effect of GnRH administered 34 h after P4 insert removal (GnRH34) on time of ovulation. Suckled cows (n = 34) were treated with 2 mg estradiol benzoate (EB) and an intravaginal insert containing 1.9 g of P4. Eight days later, P4 inserts were removed, and all cows received 150 μg of d-cloprostenol (prostaglandin F2 alpha analogue), 300 IU of eCG, and 1 mg of estradiol cypionate (ECP). On Day 9 (05:00 p.m.), cows were randomly distributed, according to the diameter of the pre-ovulatory follicle, in two treatments: 1) GnRH (n = 17) cows that received 10.5 μg of buserelin acetate, or 2) no further treatment (control, n = 17). Cows treated with GnRH 34 h after P4 insert removal ovulated earlier (P = 0.02) than control cows (66 ± 0.0 and 77.2 ± 4.3 h). Experiment 2 was designed to determine the effect of GnRH34 on the fertility of suckled beef cows. Nelore cows (n = 506) were treated as Experiment 1. On Day 8, cows were painted in the sacrocaudal region to identify cows that displayed estrus. On Day 9 (05:00 p.m.), cows were randomized to receive same treatment as Experiment 1, control (n = 252), or GnRH (n = 254). All cows were TAI 48 h after P4 insert removal. At TAI, estrus was evaluated, and deemed to have occurred in cows without a tail-head chalk mark (>75% paint loss). Cows treated with GnRH 34 h after P4 insert removal had greater (P = 0.01) pregnancy per AI (P/AI) than cows that only received ECP (63.0% and 50.4%). No difference (P = 0.5) was observed in the proportion of cows that displayed estrus between treatments. Furthermore, cows that displayed estrus had greater (P < 0.01) P/AI than cows that did not. Treatment with GnRH, given at 34 h after P4 insert removal, increased (P < 0.05) P/AI in cows that did not show estrus at TAI. In summary, treatment with GnRH 34 h after P4 insert removal was associated with earlier ovulation and resulted in greater P/AI in suckled Nelore cows treated with an estradiol/P4-based protocol for TAI.
Veronica Hoyos-Marulanda, Cristina S. Haas, Karina L. Goularte, Monique T. Rovani, Rafael G. Mondadori, Arnaldo D. Vieira, Bernardo G. Gasperin, and Thomaz Lucia
Cambridge University Press (CUP)
Summary Regulation of the transforming growth factor beta (TGFβ) superfamily by gonadotrophins in swine follicular cells is not fully understood. This study evaluated the expression of steroidogenic enzymes and members of the TGFβ superfamily in prepubertal gilts allocated to three treatments: 1200 IU eCG at D −3 (eCG); 1200 IU eCG at D −6 plus 500 IU hCG at D −3 (eCG + hCG); and the control, composed of untreated gilts. Blood samples and ovaries were collected at slaughter (D0) and follicular cells were recovered thereafter. Relative gene expression was determined by real-time PCR. Serum progesterone levels were greater in the eCG + hCG group compared with the other groups (P < 0.01). No differences were observed in the expression of BMP15, BMPR1A, BMPR2, FSHR, GDF9, LHCGR and TGFBR1 (P > 0.05). Gilts from the eCG group presented numerically greater mean expression of CYP11A1 mRNA than in the control group that approached statistical significance (P = 0.08) and greater expression of CYP19A1 than in both the eCG and the control groups (P < 0.05). Expression of BMPR1B was lower in the eCG + hCG treatment group compared with the control (P < 0.05). In conclusion, eCG treatment increased the relative expression of steroidogenic enzymes, whereas treatment with eCG + hCG increased serum progesterone levels. Although most of the evaluated TGFβ members were not regulated after gonadotrophin treatment, the downregulation of BMPR1B observed after treatment with eCG + hCG and suggests a role in luteinization regulation.
Andrez Pastorello Bohn, Arnaldo Diniz Vieira, Thomaz Lucia Junior, Bernardo Garziera Gasperin, and Rafael Gianella Mondadori
Editora da Universidade Federal Rural do Semi-Arido - EdUFERSA
Embryo cryopreservation methods have been used for commercialization and formation of genetic banks. Cryopreservation of equine embryos <300 μm in diameter, collected at days 6-6.5 after ovulation, allows satisfactory pregnancy rates. However, higher embryo collection rates in mares are obtained when uterine flush is performed between days 7 and 8 after ovulation when embryos are >300 μm in diameter, needing blastocoel collapse for satisfactory resistance to cryopreservation by vitrification. To evaluate the viability of simplified blastocoel collapse by embryo puncture with low technology and low-cost equipment, 22 embryos, collected at day 8 post-ovulation (D8), were allocated to the following groups: (1) micropuncture with a 30 G needle, assisted by a mechanical micromanipulator, before vitrification (n=4); (2) manual blade microsection before vitrification (n=6); (3) no manipulation prior to vitrification (n=8); and (4) freshly inovulated embryos (n=4). Despite the high re-expansion rates observed after vitrification, embryos manipulated prior to vitrification (groups MP and MS) did not result in pregnancy 25 days after transfer. On the other hand, embryos from groups NM (non-micromanipulated) and FR (freshly inovulated) resulted in pregnancies at 25 days. Under the conditions of the present study, manual blastocoel collapse was not efficient in increasing cryotolerance to vitrification among large embryos, requiring improvements to obtain pregnancies.
Fabiane Pereira de Moraes, Daniele Missio, Jessica Lazzari, Monique Tomazele Rovani, Rogério Ferreira, Paulo Bayard Dias Gonçalves, and Bernardo Garziera Gasperin
FapUNIFESP (SciELO)
Abstract The identification of mutations in the genes encoding bone morphogenetic protein 15 (BMP15) and growth and differentiation factor 9 (GDF9) associated with phenotypes of sterility or increased ovulation rate in sheep aroused interest in the study of the role of local factors in preantral and antral folliculogenesis in different species. An additive mutation in the BMP15 receptor, BMPR1b, which determines an increase in the ovulatory rate, has been introduced in several sheep breeds to increase the number of lambs born. Although these mutations indicate extremely relevant functions of these factors, the literature data on the regulation of the expression and function of these proteins and their receptors are very controversial, possibly due to differences in experimental models. The present review discusses the published data and preliminary results obtained by our group on the participation of local factors in the selection of the dominant follicle, ovulation, and follicular atresia in cattle, focusing on transforming growth factors beta and their receptors. The study of the expression pattern and the functionality of proteins produced by follicular cells and their receptors will allow increasing the knowledge about this local system, known to be involved in ovarian physiopathology and with the potential to promote contraception or increase the ovulation rate in mammals.
Wagner Marques Lima, Fabiane Pereira de Moraes, Rogério Ferreira, Rafael Gianella Mondadori, Arnaldo Diniz Vieira, Nathália Wacholz Knabah, Danylo Cintra Medeiros Lima, Monique Tomazele Rovani, Luiz Francisco Machado Pfeifer, Paulo Bayard Dias Gonçalves,et al.
FapUNIFESP (SciELO)
Abstract Estradiol cypionate (EC) or GnRH have been widely used for ovulation induction in timed embryo transfer (TET). EC administration increases the proportion of cows that show estrus, whereas GnRH promotes more synchronized ovulations. The aim of the present study was to evaluate the potential beneficial effects of combining EC and GnRH in TET. In experiment 1, no difference was observed on serum progesterone concentrations on Day 6 and 13 after GnRH treatment between GnRH and EC+GnRH groups. In experiment 2, pregnancy per embryo transfer (P/ET) did not differ (p = 0.69) between GnRH (62.8%) and EC+GnRH (58.7%) groups. In conclusion, combining EC and GnRH for ovulation induction does not increase progesterone secretion and pregnancy rate after TET in cattle.