Bernardo Garziera Gasperin

Scopus Publications

Contraceptive Strategies for Female Cattle: Efficacy and Zootechnical Aspects
Daniela Guarchez Adamich, Lucas Cardoso Azeredo, Fabiane Pereira de Moraes, Daniele Missio, Fernando Caetano de Oliveira, and Bernardo Garziera Gasperin
Wiley
ABSTRACTUnwanted pregnancies at the slaughterhouse are a recurring problem globally, compromising ethical aspects and animal welfare, and causing production losses. This review explores contraceptive strategies for female cattle, focusing on both management practices and suppression of the estrous cycle and/or fertilisation. Contraceptive techniques such as surgical castration, emasculation of the ovaries with rubber rings, intrauterine devices (IUDs), GnRH agonist implants, and immunocastration are discussed. Surgical castration, although efficient, is an invasive procedure that compromises animal welfare. Alternative methods, such as IUDs and GnRH implants, lack commercially available products and large‐scale efficacy studies. Immunocastration is easy to apply and does not require specialised equipment, but also requires further studies to evaluate its effects on animal performance. Despite the various contraceptive alternatives available, the high number of pregnant females slaughtered highlights the need for awareness among producers and technicians, as well as more in‐depth studies on strategies that can promote benefits to animals and production.

Luteal Function and Pregnancy Rate in Bos taurus Heifers Treated With GnRH Prior to Timed Artificial Insemination
Julia Nobre Blank Camozzato, Bruna Favretto de Souza, João Marcello Rodrigues, Alinne Machado Petrarca Léo, Luiz Francisco Machado Pfeifer, Marcelo Bertolini, Monique Tomazele Rovani, Rafael Gianella Mondadori, Bernardo Garziera Gasperin, and Fernando Caetano de Oliveira
Wiley
ABSTRACTThe study aimed to evaluate the effects of GnRH given 34 h after progesterone (P4) intravaginal device (IVD) removal in a timed artificial insemination (TAI) protocol. In Experiment 1, 17 Hereford and Braford heifers (control, n = 7; GnRH34; n = 10) received 2 mg estradiol benzoate (EB) and a 1 g P4 IVD on Day 0 (D0). On D8, IVDs were removed and 150 μg d‐cloprostenol (PGF) and 1 mg estradiol cypionate (EC) were administered. In the GnRH34 group, animals received 25 μg lecirelin 34 h after the IVD removal (D9). TAI was performed in both groups on D10 (48 h after IVD removal). Follicular dynamics were evaluated from D8 until ovulation; blood samples were collected 7 and 12 days after TAI. In Experiment 2, to evaluate the effect of GnRH34 on pregnancy rates (control, n = 187; GnRH34; n = 203), heifers were subjected to the TAI protocols described in Experiment 1. No significant differences were observed in preovulatory follicle (POF) diameter on D10, follicular growth and ovulation rates until 72 h and P4 concentrations on days 7 and 12 after TAI (p > 0.05). Pregnancy rates (PR) were similar between groups (Control = 50.2%; GnRH34 = 50.2%; p = 0.65). A significant effect of body weight (BW) and body condition score (BCS) on PR was observed for control (p< 0.05), but not for GnRH34. In conclusion, GnRH 34 h after the IVD removal in beef heifers previously treated with EC had no effect on luteal function and fertility.

NPR3 is regulated by gonadotropins and modulates bovine cumulus cell expansion
Matheus P De Cesaro, Mariana P de Macedo, Paulo R A da Rosa, Joabel T dos Santos, Ricardo D Mea, Janduí E da Nóbrega, Raj Duggavathi, Bernardo G Gasperin, Paulo Bayard D Gonçalves, and Vilceu Bordignon
Bioscientifica
In briefThe natriuretic peptide system, particularly the C-type natriuretic peptide (CNP) and natriuretic peptide receptor 2 (NPR2), plays a critical role in regulating mammalian ovarian functions, including oocyte–cumulus cell communication and meiotic maturation. However, the contribution of natriuretic peptide receptor 3 (NPR3) to these processes has not been thoroughly investigated. Data from this study provide compelling evidence that NPR3 is involved in modulating gonadotropin signaling and regulating cumulus cell expansion in cattle.AbstractThe significant role of CNP and its receptor 2 (NPR2) in regulating oocyte meiotic maturation and facilitating communication between oocytes and surrounding cumulus cells has been well documented in various mammalian species including mice, cattle and swine. However, further investigation is needed to ascertain whether natriuretic peptide receptors (NPRs) are involved in regulating other essential ovarian functions. Hence, this study aimed to explore the potential involvement of NPRs in the regulation of cumulus expansion and oocyte meiotic maturation in bovine cumulus–oocyte complexes (COCs). The findings revealed that NPR3 mRNA abundance was downregulated by follicle-stimulating hormone and luteinizing hormone in cumulus cells of bovine COCs during in vitro maturation (IVM), while NPR2 mRNA levels were not affected by gonadotropins. Inhibition of the epidermal growth factor receptor (EGFR) during IVM of COCs prevented the NPR3 mRNA downregulation induced by gonadotropins in cumulus cells. Additionally, treatment of COCs during IVM with an NPR3 agonist (cANP4–23) inhibited cumulus expansion induced by gonadotropins. This inhibitory effect was further intensified when COCs were cotreated with cANP4–23 and CNP. These findings provide robust evidence indicating that normal cumulus expansion in bovine COCs involves an inhibitory effect of gonadotropins on NPR3 mRNA expression, which is mediated via EGFR signaling. The study also provides evidence that CNP and NPR3 interact synergistically to regulate cumulus expansion in response to gonadotropins.

Is it possible to replace eCG by GnRH in the hormonal protocol for timed artificial insemination in ewes during the breeding season?
Gabriel Maggi, Otávio S. Pires, Sérgio F. Vargas Junior, Fernando C. Oliveira, Fabiane P. Moraes, Rogério Ferreira, Arnaldo D. Vieira, Monique T. Rovani, Paulo B.D. Gonçalves, Rafael G. Mondadori,et al.
Elsevier BV

Morphometric Evaluation of the Ampullae of the Oviducts in Prepubertal Gilts Treated With Chorionic Gonadotropins
Camila R. C. Brito, Veronica Hoyos‐Marulanda, Luis A. X. Cruz, Bernardo G. Gasperin, Arnaldo D. Vieira, Rafael G. Mondadori, and Thomaz Lucia
Wiley
ABSTRACTThis study assessed morphometric traits of the ampulla of the oviducts in prepubertal gilts treated with chorionic gonadotropins. With the day of slaughter as D0, gilts were assigned to four treatments (n = 8 each): control (untreated), eCG (200 IU eCG on D3), eCG+hCG (1200 IU eCG on D6 plus 500 IU hCG on D3), and eCG+hCG+AI (the previous treatment plus artificial insemination on D1). Blood and ampullae samples were collected at slaughter. Serum progesterone concentrations were higher for gilts treated with hCG than for those in the eCG and control treatments (p < 0.001), but estradiol concentrations did not differ (p > 0.05). The epithelium, muscle and lumen areas and the inner and larger ampullae diameters did not differ across treatments (p > 0.05). Therefore, treatment with chorionic gonadotropins did not alter the ampullae morphometry of prepubertal gilts.

Thermotolerance in Angus cattle is related to hair coat characteristics but not to coat color
Caroline Oliveira Farias, Jessica Lazzari, Ísis Soares da Cunha, Paulo Bayard Dias Gonçalves, Bernardo Garziera Gasperin, Thomaz Lucia, Eduardo Schmitt, Fernando Flores Cardoso, Juliana Sarubbi, and Rafael Gianella Mondadori
Elsevier BV

Prostaglandin F2α treatment does not hasten ovulation in weaned sows
D.P. Fagundes, M.S. Lucca, B.G. Gasperin, D. Missio, M. Quirino, A.P.G. Mellagi, P.B.D. Gonçalves, F.P. Bortolozzo, and R.R. Ulguim
Elsevier BV

Thermoregulatory response of black or red lactating Holstein cows in the hot and cold season in southern Brazil
Jéssica Lazzari, José Victor Vieira Isola, Vanessa Lambrecht Szambelan, Gabriel Menegazzi, Marcos Busanello, Monique Tomazele Rovani, Juliana Sarubbi, Eduardo Schmitt, Rogerio Ferreira, Paulo Bayard Dias Gonçalves,et al.
Elsevier BV

GnRH analogs induce a LH peak and increase pregnancy per timed-AI in ewes
Raquel Grande Pereira, Otávio Saraiva Pires, Clério Antônio Hoefle, Alexandro Fritzen, Giovanna Fiordalisi, Juliana Germano Ferst, Fernando Caetano de Oliveira, Arnaldo Diniz Vieira, Rafael Gianella Mondadori, Gabriel Maggi,et al.
Elsevier BV

Use of chorionic gonadotropins during lactation to optimize postpartum sow reproductive performance: a review
Monike Willemin Quirino, Carolini Schultz, Michele dos Passos Dezordi Franz, Thomaz Lucia Jr, Arthur Martelli, Paulo Bayard Dias Gonçalves, Rafael da Rosa Ulguim, Bernardo Garziera Gasperin, and Ivan Bianchi
FapUNIFESP (SciELO)
Abstract Treating lactating sows with chorionic gonadotropins may allow controlling their post-weaning reproductive function, despite the occurrence of anestrous during lactation. This article reviews the potential effectiveness of treatment with both equine and human chorionic gonadotropins (eCG and hCG, respectively) during lactation on the control of estrus expression and ovulation in weaned sows. The use of 1,000 IU hCG at 24 and 48 h postpartum may induce ovulation in the treated sows, but the ovulation rate may be variable. Pregnancy rates may be improved with combined treatment after the second week of lactation with both chorionic gonadotropins: 1,500 IU eCG plus 500 – 1,000 hCG; or 1,000 IU eCG plus 1,000 IU hCG. Treatment with eCG (1,000 – 2,000 IU) at the end of lactation may result in acceptable estrus expression and ovulation rates, although with marginal benefit for pregnancy rates. The subsequent response to treatments with chorionic gonadotropins during lactation is likely influenced by the treatment period, the suckling frequency during lactation, and the boar exposure during the weaning-to-estrus interval. A better understanding of the efficiency of such steroid-free treatments is increasingly relevant due to the constraints of the use of steroid hormones in livestock reproductive management.

Does GnRH treatment at TAI regardless of estrus occurrence increase pregnancy rate in crossbred Bos taurus suckled cows?
Vanessa Silva Fernandes, Gabriella dos Santos Velho, Mateus Felipe Osório dos Santos, Kelly Alves Evangelista, Bernardo Garziera Gasperin, Luiz Francisco Machado Pfeifer, Rogério Ferreira, Paulo Bayard Dias Gonçalves, Gustavo Desire Antunes Gastal, André Gustavo Cabrera Dalto,et al.
FapUNIFESP (SciELO)
Abstract The impact of GnRH treatment on the day of TAI in beef cows has received limited investigation, especially concerning its association with estrus expression. Consequently, two experiments were conducted to assess the potential of GnRH treatment on the day of TAI to enhance fertility according to the expression or not of estrus in beef cows. Experiment 1 aimed to determine ovulation rate and luteal function, while Experiment 2 aimed to determine the effect of the two GnRH treatment approaches on pregnancy rate. In Experiment 1, multiparous Brangus suckling cows (n = 17) were submitted to an 8-day TAI protocol. Estrus occurrence was evaluated based on chalk removal on D10 (TAI) and cows were assigned to receive GnRH (25µg lecirelin; im) according to the group: GnRH (n = 7), regardless of estrus expression; or selectGnRH (n = 10), only cows not detected in estrus. Ovulation rate occurring until 77h after IVD removal did not differ (p = 0.17) between GnRH (85.7%; 6/7) and selectGnRH (100%; 10/10). Also, corpus luteum size and serum progesterone concentration were not affected (p>0.05) by treatments. In Experiment 2, crossbred taurine suckled cows (n = 384) were submitted to the same protocol as described in Experiment 1 and were randomly allocated to GnRH or selectGnRH groups. There was no difference in P/AI between groups (selectGnRH = 55.6%; GnRH = 54.3%; p = 0.7) 30 days after TAI. As expected, there was a pronounced effect (p<0.0001) of estrus expression on P/AI (Estrus = 61.5%; No estrus = 33.0%), regardless of group. In summary, ovulation timing and rate and luteal function did not differ between groups. Also, GnRH administration only in cows that do not show estrus is recommended, considering hormone savings and similar conception rate.

An efficient shorter protocol for artificial induction of lactation in heifers
José Victor Vieira Isola, Henrique Schneider Pestano, Sergio Farias Vargas Junior, Fernando Caetano de Oliveira, Fabiane Pereira de Moraes, Camila Ribeiro Carvalho de Brito, Lucas Lourenço Castiglioni Guidoni, Rogério Ferreira, Arnaldo Diniz Vieira, Monique Tomazele Rovani,et al.
FapUNIFESP (SciELO)
ABSTRACT: Protocols to artificially induce cows and heifers into lactation are effective and commercially available in Brazil. However, these protocols demand long hormonal treatments, which is debatable since little research has been done in the subject. To further understand artificial induction to lactation (AIL) protocols, we conducted two experiments. In experiment 1, our objective was to better characterize steroidal profile during an AIL protocol and assess the estradiol (E2) and progesterone (P4) serum profiles of Jersey cows (n=6) subjected to a conventional protocol. In experiment 2, we aimed to compare milk production and serum E2 concentrations of Holstein heifers induced into lactation by the standard AIL protocol (15 injections of E2 and 8 P4 injections) and by a short protocol in which the number of E2 injections and, consequently, the overall dosage of E2, were reduced (8 injections of E2 and 8 P4 injections). We hypothesized that a short AIL protocol (8 days) would be as efficient as the long standard protocol of 15 days. Our hypothesis was confirmed, since we demonstrated that a shorter protocol was able to induce lactation in Holstein heifers without hindering milk production.

Reproductive performance in gilts submitted to non-steroidal therapies to prolong the luteal phase of the estrous cycle
Camila R.C. Brito, Ágatha D. Cordeiro, Pricila Baldessar, Carolini Schultz, Monike Quirino, Rafael R. Ulguim, Paulo B.D. Gonçalves, Thomaz Lucia, Ivan Bianchi, and Bernardo G. Gasperin
Elsevier BV

Vitamin E reduces the reactive oxygen species production in dominant follicle during the negative energy balance in cattle
Daniele Missio, Fabio Gallas Leivas, Francielli Cibin, Tatiana Emanuelli, Sabrina Somacal, Vanessa Buss, Bernardo Gasperin, Dimas Estrasulas de Oliveira, Paulo Bayard Dias Gonçalves, and Rogério Ferreira
Wiley
AbstractIn the postpartum period, there is an increase in non‐esterified fatty acids (NEFA) in both serum and follicular fluid (FF) of cattle. The increase in fatty acid concentration results in increased production of reactive oxygen species (ROS) that can compromise bovine fertility. The objectives of this study were to characterize the lipid profile found in the FF of cows experiencing induced negative energy balance (NEB) and to evaluate the effect of α‐tocopherol in the prevention of oxidative stress in the serum and FF of cows. Twenty‐nine beef cows were divided into groups: (1) control; (2) Fasting for 24 days; and (3) Fasting + VitE. Between D0 and D4 blood samples were taken to assess concentrations of NEFA, ROS production, total antioxidant capacity (FRAP), lipid peroxidation, and α‐tocopherol (vitamin E). On D4, follicular aspiration was performed for analysis of FF from the dominant follicle. Our results demonstrate that fasting was effective in causing increased fat mobilization in animals. The increase in serum concentration of C18:1c9 was reflected in the FF of fasting cows. Serum α‐tocopherol concentration was higher in the control and Fasting + VitE groups compared to the Fasting group. In FF, there was an increase of α‐tocopherol in the Fasting + VitE group in comparison to Fasting cows. There was an increase in ROS production in the serum of fasting cows. ROS production in FF was higher in the Fasting compared to the Fasting + VitE group. Vitamin E has beneficial effects in reducing ROS production in the dominant follicle of cows in NEB.

GnRH34 with or without estradiol cypionate in timed AI in Bos indicus beef cows
Samira A. Silva, Rafael G. Mondadori, Gabrielly S. Noleto, Ingrid P. Barbosa, Reuel L. Gonçalves, Bernardo G. Gasperin, Monique T. Rovani, Eanes F. Paz, Leonardo S. Gomes, and Luiz F.M. Pfeifer
Elsevier BV

17α-estradiol does not adversely affect sperm parameters or fertility in male mice: implications for reproduction-longevity trade-offs
J. Isola, G. B. Veiga, Camila R C de Brito, J. A. Alvarado-Rincón, D. N. Garcia, B. Zanini, Jéssica D Hense, A. D. Vieira, M. Garratt, B. Gasperin,et al.

Long-acting injectable progesterone treatment prior to puberty induction in gilts
P. Baldessar, C.R.C. de Brito, H. Johann, C. Schultz, V. Peripolli, F. Moreira, T. Lucia, R.R. Ulguim, B.G. Gasperin, and I. Bianchi
Elsevier BV

Successful lactation induction in non-pregnant gilts
Ágatha Decroix Cordeiro, Ana Júlia B. Conterato, Ivan Bianchi, Débora L. A. Dartora, Ricardo Zanella, Carlos Bondan, Mariana G. Marques, Bernardo G. Gasperin, and Thomaz Lucia
Wiley
A protocol to induce lactation was applied to non-pregnant gilts. In Experiment I, five gilts with estrus synchronized through oral supplementation of 20 mg Altrenogest for 18 d received: 10 mg estradiol cypionate (EC) on the last day of estrus expression (D0); 10 mg EC and 300 mg long-acting progesterone (P4) on D26; and two 0.53 mg doses of a prostaglandin F2α analogue (PGF) 12 h apart on D36. Blood was collected on D12, D19, D26 and D33. Milk secretion started in all gilts 24 h after PGF administration and lasted at least 8 d. Milk samples were collected from D37 to D45. The serum P4 concentration was lower on D12 than subsequently (P < 0.05), but the estradiol concentration was unaltered (P > 0.05). The milk produced during the induced lactation was generally richer in protein and poorer in fat compared to the milk from the lactation of a reference sow. In Experiment II, the same protocol induced lactation in two gilts, which nursed fostered piglets for 22 d. Thus, lactation was induced in all treated gilts and the milk produced was capable to nurture fostered piglets.

Association between chronic Anaplasma marginale and Babesia spp. infection and hematological parameters of taurine heifers
Natalia Machado Rahal, Gabriela Bueno Luz, Kauê Rodriguez Martins, Bernardo Garziera Gasperin, Josiane de Oliveira Feijó, André Gustavo Cabrera Dalto, Monique Tomazele Rovani, Rodrigo Casquero Cunha, and Marcio Nunes Corrêa
FapUNIFESP (SciELO)
Abstract The aim of this study was to investigate the association between chronic Anaplasma marginale and Babesia spp. infection and hematological parameters of pregnant and non-pregnant taurine heifers. Blood samples from 94 females were collected on the first day (D-10) of timed artificial insemination (TAI) protocol and on pregnancy diagnosis (D+34). Hematological parameters were determined and compared between pregnant (PG) and non-pregnant (NPG) heifers, and within group at different sampling days. Real-time PCR (qPCR) was used to determine A. marginale and Babesia bovis infection, and for absolute quantification of Babesia spp. between PG and NPG groups. Correlation analysis was performed between the number of gDNA copies (CN) of Babesia spp. and hematological parameters. On D-10, mean hemoglobin concentration was higher for NPG, and hematocrit and total plasma protein were higher on D+34 for both groups. There was no difference in Babesia spp. CN between groups. In the first qPCR, all heifers were positive for A. marginale and B. bovis. Significant correlations were found between hemoglobin and erythrocyte and between hemoglobin and hematocrit (r = 0.8082 and r = 0.3009, respectively). Low levels of A. marginale and Babesia spp. did not affect hematological parameters of chronically infected pregnant and non-pregnant taurine heifers.

Analysis of nuclear maturation, DNA damage and repair gene expression of bovine oocyte and cumulus cells submitted to ionizing radiation
Bruno Tomazele Rovani, Vitor Braga Rissi, Monique Tomazele Rovani, Bernardo Garziera Gasperin, Tadeu Baumhardt, Vilceu Bordignon, Liliane de Freitas Bauermann, Daniele Missio, and Paulo Bayard Dias Gonçalves
FapUNIFESP (SciELO)
Abstract Radiotherapy causes destruction of tumor cells, but also threatens the integrity and survival of surrounding normal cells. Then, woman submitted to irradiation for cancer treatment may present permanent ovary damage, resulting in impaired fertility. The objective of this study was to investigate the effects of therapeutic doses of ionizing radiation (IR), used for ovarian cancer treatment in humans, on bovine cumulus-oocyte complexes (COCs) as experimental model. Bovine ovaries were exposed to 0.9 Gy, 1.8 Gy, 3.6 Gy or 18.6 Gy IR, and then COCs were collected and used to evaluate: (a) oocyte nuclear maturation; (b) presence of phosphorylated H2A.X (γH2AX), as an indicator of DNA double-strand breaks (DSBs); and (c) expression of genes involved in DNA repair (TP53BP1, RAD52, ATM, XRCC6 and XRCC5) and apoptosis (BAX). The radiation doses tested in this study had no detrimental effects on nuclear maturation and did not increase γH2AX in the oocytes. However, IR treatment altered the mRNA abundance of RAD52 (RAD52 homolog, DNA repair protein) and BAX (BCL2-associated X protein). We conclude that although IR doses had no apparent effect on oocyte nuclear maturation and DNA damage, molecular pathways involved in DNA repair and apoptosis were affected by IR exposure in cumulus cells.

Lipopolysaccharide effects on activation and apoptosis of primordial ovarian follicles in heifers
A.S. Maffi, J.A. Alvarado. Rincón, A.A. Barbosa, M.A. Weiller, R.G. Mondadori, B.G. Gasperin, M.T. Rovani, L.A.X. Cruz, K.C. Freitas, A. Schneider,et al.
FapUNIFESP (SciELO)
ABSTRACT The objective of the present study was to evaluate the effect of lipopolysaccharide (LPS) administration on activation and apoptosis of primordial follicles. There was no difference in the total number of follicles as well as in the different types of follicles. Furthermore, the LPS challenge didn’t modulate the expression of genes related with ovarian reserve (HAM), oocyte survival (Survivin), activation rate (Pten, KIT, KITL1, KITL2, AKT1, SIRT1), and follicular abnormalities. Therefore, the LPS exposure with 24h interval had no effect on activation rate and primordial follicles abnormalities, and also had no effect on expression of anti-apoptotic genes and genes related with ovarian reserve, oocyte survival, activation rate, and primordial follicles abnormalities.

Prostaglandin F2α treatment concurrent with artificial insemination does not affect bovine embryo production
Monique Mazzarollo Frata, Wagner Marques de Lima, Danylo Cintra Medeiros Lima, Dênis Halinski da Silveira, Thomaz Lucia Júnior, Rogério Ferreira, Paulo Bayard Dias Gonçalves, and Bernardo Garziera Gasperin
FapUNIFESP (SciELO)
ABSTRACT: Treatment with prostaglandin F2α (PGF) induces ovulation and increases conception rates in cows, while improving embryo production in buffalos. However, its effect on superovulated cows is unknown. This study verified whether single PGF administration concurrent with artificial insemination (AI) improves fertilization and embryo production rates in superovulated cows. In each replicate, embryo donor cows were equally allocated to two groups: the untreated control and PGF groups. The latter of which received 482 µg of cloprostenol concurrent with the first AI. Each cow (n = 35) was subjected to two superovulations (SOV) in a crossover design (total = 70 embryo collections). In the control and PGF groups, respectively, the observed responses were [median (95% CI)]: 12 (10-18) and 15 (12-18) total structures, 9 (7-11) and 7 (6-10) viable embryos, 1 (0-1) and 1 (1-3) degenerated embryos, and 1 (0-3) and 2 (0-5) oocytes (P > 0.05). In conclusion, single PGF treatment concurrent with the first AI did not affect embryo production in superovulated cows.

Expression of genes associated with fertility in the uterus and oviduct of heifers challenged with lipopolysaccharide
Giuliana A. Ferronato, Joao A. Alvarado-Rincón, Andressa S. Maffi, Antônio A. Barbosa, Bernardo G. Gasperin, Augusto Schneider, Rafael G. Mondadori, Cássio C. Brauner, and Marcio N. Corrêa
Cambridge University Press (CUP)
Summary Lipopolysaccharide (LPS) endotoxemia has been negatively associated with fertility. This study aimed to investigate the effect of LPS-induced inflammation on gene expression associated with bovine fertility in the uterus and oviduct. Sixteen healthy heifers were divided into two groups. The LPS group (n = 8) received two intravenous (i.v.) injections of 0.5 µg/kg of body weight of LPS with a 24-h interval, and the control group (n = 8) received two i.v. injections of saline solution with the same interval of time. All the animals had the follicular wave synchronized. Three days after the second injection of LPS, all animals were slaughtered and uterine and oviduct samples were collected. Gene expression associated with inflammatory response, thermal and oxidative stresses, oviduct environment quality, and uterine environment quality was evaluated. Body temperature and leucogram demonstrated that LPS induced an acute systemic inflammatory response. In the uterus, the expression of PTGS2 and NANOG genes was downregulated by the LPS challenge. However, no change in expression was observed in the other evaluated genes in the uterus, nor those evaluated in the oviduct. In conclusion, the inflammatory process triggered by LPS did not persist in the uterus and oviduct 3 days after challenge with LPS. Nonetheless, reduction in PTGS2 and NANOG expression in the uterus suggested that, indirectly, LPS may have a prolonged effect, which may affect corpus luteum and endometrial functions.

Postponing TAI in beef cows with small preovulatory follicles
L.F.M. Pfeifer, B.G. Gasperin, J.P. Cestaro, and A. Schneider
Elsevier BV

The influence of prorenin/(pro)renin receptor on progesterone secretion by the bovine corpus luteum
Andressa Minussi Pereira Dau, Paulo Roberto da Rosa, Joabel dos Santos, Juliana Ferst, Mariana de Macedo, Monique Rovani, Fabio Comim, Alfredo Quites Antoniazzi, Bernardo Gasperin, Rogério Ferreira,et al.
Elsevier BV

Bernardo Garziera Gasperin

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Scopus Publications