Volkov
@vniro.ru
Molecular genetics
VNIRO
Scopus Publications
- Increased clonality and genetic differentiation across the Arctic Ocean in tetraploid sea anemone Aulactinia stella
Ekaterina Bocharova, Aleksandr Volkov, Solenn Stoeckel
Peer Community Journal, 2025
Reproductive mode is a key factor shaping genetic diversity, evolutionary potential, and the processes of dispersal and colonization. Clonality is particularly common in harsh environments and at the margins of species ranges, where it supports persistence, enables rapid growth, and promotes the maintenance of locally adapted genotypes. In the rapidly changing Arctic, increasing ecological connectivity is eroding historical barriers for sessile species. Evaluating genetic diversity in this context, before global change further alters Arctic ocean, is essential for understanding evolutionary dynamics during range expansion and for informing conservation strategies. Aulactinia stella is a circumpolar sea anemone with physiological characteristics in laboratory conditions suggesting a potential for clonal reproduction. In this study, we investigated its reproductive modes in natural populations across the Arctic ocean, from the northern Pacific to the Atlantic, and examined how genetic diversity is structured between adults and juveniles at five sampled sites. Across all study sites, we observed only females or individuals lacking gonads, with the exception of Kamchatka, where males were also present. Genetic indices and changes in genotype frequencies between adults and juveniles confirmed that this species reproduces partially by parthenogenesis. Populations on the Atlantic side were highly clonal with clonal rates ( c ) estimated at 80-99%, whereas populations on the Pacific side reproduced more sexually (c around 50%). Allelic diversity was twice as high in Kamchatka and Kuril populations, suggesting North Pacific coasts being the main last glacial refugia of A. stella . We found a stepping-stone pattern of genetic structure from Kamchatka to Atlantic populations, consistent with contemporary ocean currents and melted summer sea ice. Only a subset of the juvenile genetic diversity, mostly of local origin, was found in the established adults, while juveniles exhibited lower levels of genetic differentiation across the Arctic Ocean. Our findings underscore the need for further ecological and behavioral investigations to elucidate the mechanisms allowing the current possibilities of dispersal of this species across the Arctic Ocean. - Study of the Polymorphism of ISSR Markers in Spined Loaches of the Genus Cobitis (Cobitidae) in Connection with Problems of Differentiation of the Species Involved in the Formation of Polyploid Forms of Hybrid Origin and Determination of Their Taxonomic Status
A. A. Volkov, S. M. Rastorguev, E. D. Vasil’eva, V. P. Vasil’ev
Journal of Ichthyology, 2023
We have analyzed the polymorphism of ISSR fragments of DNA by polymerase chain reaction in spined loaches for the first time. The analysis has made it possible to successfully differentiate the genomes, which were presumably involved in the formation of polyploid forms of hybrid origin: Cobitis taenia; spined loaches from the Danube River basin, which are diagnosed as C. elongatoides; C. tanaitica from the Don River basin and spined loaches from Lake Sinoe, which are also assigned to the species C. tanaitica by a number of authors. The following conclusions have been made based on comparative morphological studies of previously undifferentiated forms: (1) spined loaches from Central Europe with karyotype 2n = 50, NF = 96 correspond to the diagnosis of the species C. elongatoides; this species also differs from a number of closely related species and polyploid forms in the origin of the dorsal fin in front of the base of the pelvic fins, as well as in the presence of a spot in the lower half of the caudal fin base; (2) spined loaches from Lake Sinoe and other populations of Central Europe with karyotype 2n = 50, NF = 86 are nonconspecific to C. tanaitica and should apparently be ranked as an independent species, C. megaspila. - Metrological Support of Nucleic Acid Sequence Analysis
O. N. Melkova, E. V. Kulyabina, S. Yu. Fomina, A. A. Volkov
Measurement Techniques, 2023 - MtDNA Analysis of the Upper Yenisei Grayling (Thymallus svetovidovi) and the Mongolian Grayling (Thymallus brevirostris) and Clarification of Their Ranges
E. V. Ponomareva, A. A. Volkov, M. V. Ponomareva, E. A. Shubina
Biology Bulletin, 2022 - Ural (Zhayik) River Spawning Grounds of the Sturgeon (Acipenseridae) in the Republic of Kazakhstan: Modern Situation
G. M. Shalgimbaeva, N. S. Mugue, K. B. Isbekov, A. A. Volkov, E. V. Mikodina
Journal of Ichthyology, 2022 - Secondary contact, hybridization, and diversification in Arctic charr (Salvelinus alpinus (L.) species complex) from lakes of the Norilo-Pyasinskaya water system, Taimyr: how many forms exist there?
Alexander G. Osinov, Alexander A. Volkov, Dimitri A. Pavlov
Hydrobiologia, 2022 - Comparative genetic variability of pink salmon from different parts of their range: native Pacific, artificially introduced White Sea and naturally invasive Atlantic Scottish rivers
John Gilbey, Valeriia A. Soshnina, Alexander A. Volkov, Daria A. Zelenina
Journal of Fish Biology, 2022
Trans-oceanic movement, stocking, and subsequent establishment of Pacific pink salmon (Oncorhynchus gorbuscha) into the Atlantic White Sea area has resulted in their spreading further across the northern Atlantic, with spawning being reported in a number of regions within this area. Such expansions of non-native species bring potential risks to the ecosystems in question. It has not yet been established if the spawning events of pink salmon observed are the result of self-sustaining populations in these areas, or are due to repeated invasions of strayers from the White Sea stocks. In 2017 pink salmon were observed in a number of Scottish rivers in historically large numbers. This study set out to examine genetic variation in these fish and compare this to fish in Pacific founder regions and the White Sea translocated populations. A total of 286 samples from Scotland, the Atlantic White Sea, the Pacific Okhotsk region, and Northern Pacific Bering Sea were screened using a 1018 bp sequenced region of the Cytochrome b mtDNA gene and 205 of these samples for 13 microsatellites. Significant bottleneck and founder effects were observed in the White Sea stocks in both mitochondrial and nuclear DNA, including loss of diversity and changes in haplotype and allele proportions. Scottish fish were indistinguishable from White Sea populations and as such it was not possible to determine if the fish were strayers from this region or returning fish from previous spawning events in Scotland. Therefore, while the fish caught in Scotland have their origins in the White Sea population, it may not be easy to determine whether self-sustaining populations have, or are becoming, established in the UK using genetic analysis and other techniques may need to be employed. This article is protected by copyright. All rights reserved. - European grayling phylogeographic lineages of Russian European North from barcoding DNA fragment
Bulgarian Journal of Agricultural Science, 2022 - Determination of the Maximum Length of DNA in a Polymer Based on Linear Poly(N,N-Dimethylacrylamide) Decoded with an Accuracy of 99% by Capillary Gel Electrophoresis with Laser-Induced Fluorescence
D. V. Bocharova, Ya. I. Alekseev, A. A. Volkov, G. S. Lavrov, A. G. Plugov, et al.
Journal of Analytical Chemistry, 2021
DNA sequencing is an essential task of modern molecular genetic research. Its fundamental parameter is the accuracy of decoding. In this work, we determined the maximum lengths of DNA fragments, obtained by the Sanger method and sequenced with an accuracy of 99%, in a polymer based on linear poly(N,N-dimethylacrylamide) PDMA-6 for capillaries 50 cm long to the optical window of the detector of a Nanophore 05 domestic genetic analyzer and an imported GA 3500xL genetic analyzer. The results of DNA sequencing obtained by various analysis algorithms were compared. The parameters of the analysis algorithms were selected, ensuring best results of DNA sequencing in using the PDMA-6 polymer. - Genetic contribution of domestic European common carp (Cyprinus carpio carpio) and Amur carp (Cyprinus carpio haematopterus) to the wild Vietnamese carp population as revealed by ddRAD sequencing
Artem V. Nedoluzhko, Maria V. Gladysheva-Azgari, Gulmira M. Shalgimbayeva, Alexander A. Volkov, Natalia V. Slobodova, et al.
Aquaculture, 2021
At least three subspecies of the common carp, namely, Cyprinus carpio carpio, C. carpio haematopterus, and C. carpio viridiviolaceus, have been described to date. These subspecies natively inhabit Ponto-Caspian and Far Eastern regions, as well as northern Vietnam, but they have also been introduced into aquaculture in almost every region of the world where the environmental conditions are suitable. Moreover, common carp is one of the most widely cultured freshwater fish species in the world. In northern Vietnam, common carp fisheries and aquacultural production are of great economic importance. Investigation of the subspecies distribution among aquacultured and wild common carps is important for understanding the impact of human economic activity, such as aquaculture production, on the environment. In the present study, double-digest restriction site-associated DNA sequencing (ddRAD-seq) was used to estimate the genetic impact of aquaculture carps on the wild common carp populations of northern Vietnam. Our work reveals that a part of wild carp populations has a significant contribution to farmed fish ancestry. For some populations, this contribution exceeds 25% of the population ancestry, but the ancestry value is distributed across most specimens of the populations, whereas pure aquaculture specimens are quite uncommon for wild habitats. - Walleye pollock gadus chalcogrammus, a species with continuous range from the norwegian sea to korea, japan, and california: New records from the siberian arctic
Alexei M. Orlov, Maxim O. Rybakov, Elena V. Vedishcheva, Alexander A. Volkov, Svetlana Yu. Orlova
Journal of Marine Science and Engineering, 2021 - Genetic investigation of aral wild common carp populations (Cyprinus carpio) using ddrad sequencing
Gulmira Shalgimbayeva, Alexander Volkov, Natalia Slobodova, Fedor Sharko, Svetlana Tsygankova, et al.
Diversity, 2021 - Landscape and Climate Role in the Formation of Sturgeon Reproduction Biotopes in the Ural River (Zhaiyk)
Ekaterina V. Mikodina, Gulmira M. Shalgimbaeva, Alexander A. Volkov
E3s Web of Conferences, 2021 - Genetic Analyzer Nanofor 05 as a Measuring Instrument for DNA Sequencing
A. A. Volkov, I. A. Volkov, A. G. Plugov, E. V. Kulyabina, O. N. Melkova, et al.
Measurement Techniques, 2021 - Charrs of the genus Salvelinus (Salmonidae): hybridization, phylogeny and evolution
Alexander G. Osinov, Alexander A. Volkov, Nikolai S. Mugue
Hydrobiologia, 2021 - Spatial distribution, host specificity and genetic diversity of Onchobothrium antarcticum in the Southern Ocean
Polish Polar Research, 2021 - On the Origin of Two Species of Charrs (Salvelinus, Salmonidae) in Lake Elgygytgyn: Data on the ATPase6–NADH4L Region of Mitochondrial DNA
A. G. Osinov, A. A. Volkov
Journal of Ichthyology, 2020 - A new strain group of common carp: The genetic differences and admixture events between Cyprinus carpio breeds
Artem V. Nedoluzhko, Natalia V. Slobodova, Fedor Sharko, Gulmira M. Shalgimbayeva, Svetlana V. Tsygankova, et al.
Ecology and Evolution, 2020 - Genetic Variability of Dolly Varden (Salvelinus malma), White-Spotted Char (S. leucomaenis), and Interspecific Hybrids from the Utkholok River (Northwestern Kamchatka)
M. A. Gruzdeva, A. V. Semenova, K. V. Kuzishchin, E. V. Ponomareva, A. A. Volkov, et al.
Russian Journal of Genetics, 2020 - Author Correction: Gene-associated markers provide tools for tackling illegal fishing and false eco-certification (Nature Communications, (2012), 3, 1, (851), 10.1038/ncomms1845)
Einar E. Nielsen, Alessia Cariani, Eoin Mac Aoidh, Gregory E. Maes, Ilaria Milano, et al.
Nature Communications, 2019 - Inter- and Intra-Species Relationships of Greenland Halibut Reinhardtius hippoglossoides (Pleuronectidae) Based on the Analysis of Nuclear and Mitochondrial Genetic Markers
S. Yu. Orlova, A. A. Volkov, D. M. Shcepetov, O. A. Maznikova, N. V. Chernova, et al.
Journal of Ichthyology, 2019 - A Rare Case of Permanent Introgressive Hybridization in Char of the Genus Salvelinus (Salmonidae: Salmoniformes) in the Utkholok River, Western Kamchatka
M. A. Gruzdeva, K. V. Kuzishchin, A. V. Semenova, E. V. Ponomareva, A. A. Volkov, et al.
Russian Journal of Marine Biology, 2018 - Diversity of the CO1 Gene of Mitochondrial DNA in Representatives of Genus Antimora (Moridae, Gadiformes) in the World Oceans
S. Yu. Orlova, A. A. Volkov, I. I. Gordeev, A. A. Baitaliuk, A. M. Orlov
Doklady Biochemistry and Biophysics, 2018 - On the Origin of the Lacustrine Charr Salvelinus alpinus Complex from the Kolyma and Sea of Okhotsk Basins
A. G. Osinov, D. A. Pavlov, A. A. Volkov
Journal of Ichthyology, 2018 - Identification of microsatellite loci in sea anemones Aulactinia stella and Cribrinopsis albopunctata (family Actiniidae).
Ekaterina S. Bocharova, Alexey A. Sergeev, Aleksandr A. Volkov
F1000research, 2018
