Plant biotechnology, Green synthesis nanoparticles, plant molecular biology.
11
Scopus Publications
182
Scholar Citations
7
Scholar h-index
6
Scholar i10-index
Scopus Publications
SILVER NANOPARTICLES AND NPK FERTILIZER EFFECTS ON THE PROLINE, PEROXIDASE, AND CATALASE ENZYMES IN WHEAT WJ ATIA Sabrao Journal of Breeding and Genetics, 2024 W.J. ATIA and A.G. ORAIBI Citation: Atia WJ, Oraibi AG (2024). Silver nanoparticles and NPK fertilizer effects on the proline, peroxidase, and catalase enzymes in wheat. SABRAO J. Breed. Genet. 56(6): 2405-2415. http://doi.org/10.54910/sabrao2024.56.6.22. Summary This research investigated the effects of bio-silver nanoparticles (AgNPs) on proline content, peroxidase, and catalase enzyme
A comparative study of the Anti-Diabetic effects of avocado seed extracts and biosynthesized silver nanoparticles in Alloxan-Induced diabetic Albino mice Orooba Al-Hammood, Shaymaa H. AlRajhi, Asma G. Oraibi, Mohammed Kadhom Results in Chemistry, 2024 In this research, a comparative study of the anti-diabetic effects of Avocado (Persea americana) seed extracts and biosynthesized silver nanoparticles (Ag NPs) in alloxan-induced diabetic albino mice was conducted. The animals were assigned into twenty-one groups of five animals each; the diabetes was induced by intraperitoneal injection of 150 mg/kg of the prepared alloxan monohydrate. After 15–25 days of using 7.5 mg/ml aqueous extract of P. americana seeds, it was found that blood sugar levels dropped significantly. In addition, over the 20–25 days treatment period, levels of blood sugar were reduced considerably when doses of 5.0 or 10 mg/ml of the aqueous extract were introduced. However, throughout this treatment period, animals injected with 2.5, 5.0, 7.5, or 10.0 mg/ml of P. americana seed aqueous extract showed a significant rise in weight. In addition, over 20–25 days of treatment, animals treated with 7.5 and 10.0 mg/ml P. americana seed alcoholic extract showed a substantial decrease in blood sugar levels. Over the 25 days of treatment, animals treated with 7.5 mg/ml P. americana seed in alcoholic extract also showed a noticeable rise in weight. On the other hand, the prepared Ag NPs were synthesized and characterized using color changes, UV–visible spectroscopy, SEM, and AFM analysis. A substantial reduction in blood sugar levels was noted with 2.5 mg/ml of biosynthesized Ag NPs, while mice weights significantly increased. The highest substantial reduction in glycaemia occurred by combining 7.5 mg/ml aqueous extract and 2.5 mg/ml biosynthesized Ag NPs, resulting in a high increase in animal weight across all combination concentrations. Regarding the interaction between combination concentrations and treatment periods, animals’ weight increased significantly in all combined concentrations during the 20–25 days compared to the control. Furthermore, an increase in LDL and VLDL concentrations was observed in the negative control group compared to the positive control and other treated groups.
INCREASING SOME FLAVONOIDS COMPOUNDS FOR ECHINACEA PURPUREA L. USING COPPER OXIDE NANOPARTICLES IN VITRO. M. H. Ahmed, Z. S. Omran, A. G. Oraibi Iraqi Journal of Agricultural Sciences, 2024 This study was aimed to increase some flavonoids active compounds production in Echinacea purpurea seedlings through plant seeds treatment with different concentrations of copper oxide nanoparticles that daignosed and characterized using AFM technique. This research was implemented at plant tissue culture labrotary of College of biotechnology - Al Nahrain University, during the period of 2021 and 2023. The experiment designed factorial within CRD using three factors and ten replicates (3X3). Sodium hypochlorite concentration (S1, S2, S3, S4) (0.0, 1, 2, 3%) represented the first factor, treatment duartion time (T1, T2, T3) (5, 10, 15min) represented the second factor and copper oxide nanoparticles concentrations (C1, C2, C3, C4) (0.0, 25, 50, 75mg/ml) represented the third factor. Results showed that the full reduction in the contamination rate of the selected E. purpurea explant recorded in 3% sodium hypochlorite at 10 and 15min. The results also showed that there were a significant increase in the shoots numbers in 50mg/ml CuNPs that recording the highest shoots numbers, the shoot length increased significantly within the 25mg/ml recording 13.5cm then decreased in 50 and 75 mg/ml and the seedlings dry weight increased significantly up to 50mg/ml CuNPs that recording the highest seddlings dry weight, then the seedlings dry weight also decreased significantly in 75mg/ml CuNPs. Also, all the analyzed flavonoids compounds using HPLC device as Echinolone, Humulene, Coumarin, Myricetin, Heperidin and Naringin concentrations were significantly increased in 50 and 75 mg/L-1 Cu ONPs, except the Humulene and Coumarin compound that significantly decreased in 75 mg/L-1 Cu ONPs in comparsion to the control.
Antibacterial and Cytotoxic Activity of Mentha arvensis L. Leaves Methanolic Extract In vitro Bushra Hindi Saleh, Dhafar N. Al-ugaili, Asma G. Oraibi, Rasha Abdalelah Ibrahim Journal of Pure and Applied Microbiology, 2023 Treatment with herbal drugs is considered one of the oldest and safest methods for treating various diseases. Mentha arvensis is a medicinal plant used worldwide in the pharmaceutical industry for its antibacterial, anticancer, antiallergic, and anti-inflammatory properties. In the present study, the antibacterial activity of the methanolic extract of M. arvensis leaves was tested at different concentrations (50, 100, 150, and 200 µg/ml) against bacteria isolated from human burn wound infections, including Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus. The results demonstrated that at concentrations of 100, 150, and 200 µg/ml, the methanolic extract exhibited strong antibacterial activity against S. aureus and P. aeruginosa, while K. pneumoniae isolates were resistant to the extract at all tested concentrations. Further, the cytotoxic activity of the methanolic extract was assessed at various concentrations (100, 140, 160, 180, 220, and 250 µg/ml) against two cell lines: the RD tumor cell line and Vero normal cell line. The higher concentrations of the extract (220 and 250 µg/ml) showed potent cytotoxic effects on the RD cell line after 72 hours in a dose- and time-dependent manner. In contrast, the extract exhibited lower cytotoxic effects on Vero cells at the same concentrations after 72 h.
Carum carvi mediated green synthesis of copper nanoparticles and its effect on Solanum lycopersicum seedlings A. G. Oraibi, Alaa A. Rashad, Maysaa H. Ahmed Journal of Aridland Agriculture, 2023 The present study aims to synthesis the copper nanoparticles (CuNPs) and their effect on the biochemical and physiological characteristics of Solanum lycopersicum. The results exhibited the color change in the Carum carvi aqueous extract from clear deep brown to a greenish color and this observation indicated the green-biosynthesis of CuNPs by reduction of Cu+ to Cu0. Also, the absorbance broadening band for the green-biosynthetic CuNPs appeared at the 340 nm wavelength using UV-Vis but the C. carvi aqueous extract not showed any peaks at this wavelength. SEM analysis exhibited the micrographic surface morphology and the shape of the green-biosynthetic CuNPs with a scan area of 50 μm and showed the spherical shape particles of CuNPs aggregation. The three-dimensional image and the surface morphology of green-biosynthesized CuNPs and C. carvi aqueous extract were examined using AFM analysis that showed the surface of C. carvi aqueous extract was 45.5 nm size with non-homologus and irregular form of distribution, but the green-biosynthesized CuNPs were 12.4 nm size in nanoscale with regular and homogenous distribution form. The results also showed that the effect of bio-synthesized copper nanoparticles was evident on the S. lycopersicum seedlings fresh and dry weight according to the different reading times after treatment with nanoparticles. Also, the concentration of 2.5 mg/mL (CuNPs) showed a significant increase in the chlorophyll content (58.51 μg/cm²) on the 21st day after treatment and a significant increase in the activity of peroxidase enzyme (35.12 U min-1 mg-1 protein) was obtained at the concentration of 2.5 mg/mL (CuNPs) at 21st day after germination.
Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City Zahraa A. AlShaheeb, Zaid A. Thabit, Asma G. Oraibi Baghdad Science Journal, 2023 This study is aimed to Green-synthesize and characterize Al NPs from Clove (Syzygium aromaticumL.) buds plant extract and to investigate their effect on isolated and characterized Salmonella enterica growth. S. aromaticum buds aqueous extract was prepared from local market clove, then mixed with Aluminum nitrate Al(NO3)3. 9 H2O, 99.9% in ¼ ratio for green-synthesizing of Al NPs. Color change was a primary confirmation of Al NPs biosynthesis. The biosynthesized nanoparticles were identified and characterized by AFM, SEM, EDX and UV–Visible spectrophotometer. AFM data recorded 122nm particles size and the surface roughness RMs) of the pure S. aromaticum buds aqueous extract recorded 17.5nm particles size, while the results of Al NPs in the tested sample recorded 21nm particles size with surface roughness RMs about 2.35nm. SEM images revealed the presence of Al NPs with diameters ranged from 33.5-70.4nm with regular spiracles shape particles in the prepared biosynthesized nanoparticle sample. The EDX spectrum analysis showed that the Aluminium weight ratio was 1.75, while it was 50.498 in the Al NPs sample prepared from aqueous extract. UV-Visible spectroscopy data revealed that biosynthesized Al NPs were absorbed at 213nm while Aluminum nitrate was absorbed at 258nm. These results indicate the formation of Al NPs. The antibacterial activity showed that Al NPs exhibited having high antibacterial activity on Salmonella spp. isolates compared to the effect of the control agent (imipenem) in this experiment. We conclude that biosynthesized Al NPs from clove aqueous extract can be exploited as natural antibacterial compounds to inhibit the growth of Salmonella.
SALMONELLA ENTERICA SPECIES ISOLATED FROM LOCAL FOODSTUFF AND PATIENTS SUFFERING FROM FOODBORNE ILLNESS: SURVEILLANCE, ANTIMICROBIAL RESISTANCE AND MOLECULAR DETECTION Z. A. AlShaheeb, Z. A. Thabit, A. G. Oraibi, A. A. Baioumy, T. G. Abedelmaksoud Theory and Practice of Meat Processing, 2023 The aim of this study was to determine the prevalence of Salmonella enterica in raw chicken meat, eggs, and ready-to-eat foods containing poultry products and among patients suffering from diarrhea as a result of ingestion of this foodborne pathogen in Baghdad, Iraq. It assesses the antibiotics susceptibility, virulence and pathogenicity of S. enterica isolates. Thirteen Salmonella spp. isolates from foodstuff and seven from clinical patients were recovered from 80 and 20 samples, respectively. Isolates from foodstuff samples displayed the highest resistance to nalidixic acid (69.23%), followed by chloramphenicol (53.84%). Salmonella spp. isolated from clinical samples showed resistance to both azithromycin and cefotaxime at the same percentage level (71.42%). The results of antibiotic resistance gene amplification (gyrA, mphA) were analyzed and showed that these genes were present in 100% and 50% of phenotypically resistant isolates, respectively. Virulence genes invA, avrA, and sipB were found on average in 86% of food isolates, accounting for 69.2%, 92.3%, and 95%, respectively. In addition, the detection of these virulence genes among clinical isolates showed their presence at the same level (85.7%). Our study revealed that unhygienic chicken slaughterhouses and lack of food safety management are strong indicators of a high probability of the Salmonella presence in our food products in the Iraqi markets.
Green Biosynthesis of Silver Nanoparticles Using Malva parviflora Extract for Improving a New Nutrition Formula of a Hydroponic System A. G. Oraibi, H. N. Yahia, K. H. Alobaidi Scientifica, 2022 There are increasing needs for developing nontoxic, low-cost, high-yield, and eco-friendly procedures for manufacturing nanoparticles. Nanobiotechnology can be used in food security for improving crop production; nanoparticles could enhance the growth and yield of different crop plants; therefore, this work aimed to improve a new nutrition formula of a hydroponic system using green biosynthesis of silver nanoparticles and Malva parviflora aqueous extract. Results shown that AFM image of AgNP surface morphology provides good indicator for biosynthesizing AgNPs. UV-vis spectroscopy showed the presence of silver elements that proved the reduction of silver ion to an element in the presence of plant extract functional groups which act as a reduction reaction capping agent. AgNPs formation from 1 mM of AgNo3 and Malva parviflora filtrate can easily be characterized through visual observations by the change in the color of the reaction mixture from green to yellowish-brown. SEM showed that most of the Ag nanoparticles were spherical in shape, well dispersed, and were either arranged in clusters of particles with each other, or as small particles, and have been identified in a size range of 12–63 nm. The EDX characterization exhibited that the highest proportion of the element composition was for silver weighting (34.11%) in nanoparticle. Other elements such as aluminum (12.28%), carbon (8.62%), hafnium (18.12%), nitrogen (9.34%), sodium (10.01%), and oxygen (7.52%) may arise from Malva parviflora extract. Also, peroxidase and catalase enzyme activity, cabbage crop seedlings, fresh and dry weights, and proline and carbohydrate concentrations were significantly increased with the increase of biosynthesized AgNP concentrations but up to limit.
Phytoremediation of zinc ion using faba bean vicia faba plant in vitro Indian Journal of Ecology, 2020
Green biosynthesis, identification and characterization of ag and zn nanoparticles using ivy (epipremnum aureum) plant extract Plant Archives, 2019
RECENT SCHOLAR PUBLICATIONS
Green synthesis of gold nanoparticles using extracted, purified flavonoid from Myrtus communis Leaves. TA Mahmood, AG Oraibi مجلة مركز بحوث التقنيات الاحيائية 19 (3) , 2025 2025
A comparative study of the Anti-Diabetic effects of avocado seed extracts and biosynthesized silver nanoparticles in Alloxan-Induced diabetic Albino mice O Al-Hammood, SH AlRajhi, AG Oraibi, M Kadhom Results in Chemistry 12, 101895 , 2024 2024 Citations: 1
INCREASING SOME FLAVONOIDS COMPOUNDS FOR ECHINACEA PURPUREA L. USING COPPER OXIDE NANOPARTICLES IN VITRO. MH Ahmed, ZS Omran, AG Oraibi Iraqi Journal of Agricultural Sciences 55 (2), 733-743 , 2024 2024 Citations: 4
Silver nanoparticles and NPKK fertilizer effects on the proline, peroxidase, and catalase enzymes in wheat WJ Atia, AG Oraibi SABRAO J. Breed. Genet 56 (6), 2405-2415 , 2024 2024 Citations: 5
Synthesis of bio-active silver nanoparticles against human lung cancer cell line (a549) with little toxicity to normal cell line (WRL68) KM Talib, AG Oraibi, GI Abass Archives of Razi Institute 78 (5), 1624 , 2023 2023 Citations: 6
Effect of green-biosynthesis aluminum nanoparticles (Al NPs) on Salmonella enterica isolated from Baghdad city ZA AlShaheeb, AG Oraibi, ZA Thabit Baghdad Science Journal 20 (5 (Suppl.)) , 2023 2023 Citations: 4
Antibacterial and Cytotoxic Activity of Mentha arvensis L. Leaves Methanolic Extract In vitro. BH Saleh, DN Al-Ugaili, AG Oraibi, RA Ibrahim Journal of Pure & Applied Microbiology 17 (2) , 2023 2023 Citations: 4
Salmonella enterica species isolated from local foodstuff and patients suffering from foodborne illness: Surveillance, antimicrobial resistance and molecular detection ZA AlShaheeb, ZA Thabit, AG Oraibi, AA Baioumy, TG Abedelmaksoud Теория и практика переработки мяса 8 (2), 112-123 , 2023 2023 Citations: 7
Carum carvi mediated green synthesis of copper nanoparticles and its effect on Solanum lycopersicum seedlings AG Oraibi, AA Rashad, MH Ahmed J. Arid. Agric 9, 9-15 , 2023 2023 Citations: 7
Green Biosynthesis of Silver Nanoparticles Using Malva parviflora Extract for Improving a New Nutrition Formula of a Hydroponic System KHA A. G. Oraibi, H. N. Yahia Scientifica 2022 (special issue), 10 , 2022 2022 Citations: 13
Biosynthesizing of silver nanoparticles from Apium graveolens and Myrtus communis plants, detection, characterization and study their effect on genetic variation and growth of … OI Alwaan, IA Jdyea, AG Oraibi Baghdad, Iraq: Al-Iraqia University , 2021 2021 Citations: 6
Investigation the effects of biosynthesized silver nanoparticles on catalyase and peroxidase enzymes activity of two wheat (Triticum Aestivum L) cultivars in vitro. JA Widad, AL Wefaq, AG Oraibi EurAsian Journal of BioSciences 14 (2) , 2020 2020 Citations: 1
Impact of folair spray of biosynthesized silver nanoparticles on some physiological characteristics of two wheat (triticum aestivuml) cultivars in vivo. JA Widad, AL Wefaq, AG Oraibi EurAsian Journal of BioSciences 14 (2) , 2020 2020 Citations: 1
Molecular Identification of Str A gene responsible for streptomycin production from Streptomyces isolates MHRAGO Bayader Abdel Mohsen Pharma Innovation 9 (1), 18-24 , 2020 2020
Phytoremediation of Zinc Ion Using Faba Bean Plant Vicia faba in vitro KH Alobaidi, AG Oraibi, HN Yahya, JR Al-Obaidi Indian Journal of Ecology 47 (4), 965-968 , 2020 2020 Citations: 2
Study the Inhibitory Effect of Streptomyces spp against the Growth some Pathogenic Bacterial MHRAGO Bayader Abdel Mohsen Int.J.Curr.Microbiol.App.Sci 8 (12), 270-283 , 2019 2019
Green biosynthesis, Identification and characterization of Ag and Zn nanoparticles using Ivy (Epipremnum aureum) plant extract RIHOAG *1Hammodi H.F.2 Plant Archives 19 (2), 959-965 , 2019 2019 Citations: 4
Investigation the antibacterial activity of Portulaca oleracea L. tissue cultures in vitro A Oraibi, A AlShammari, R Mohsien, W Obaid Journal of Pharmaceutical Research International 18 (5), 1-7 , 2017 2017 Citations: 13
Investigation on the effects of UV radiation on physiological characteristics of Moringa oleifera Lam. in vitro and in situ. AG Oraibi Biotechnology Journal International 19 (4), 1-8 , 2017 2017 Citations: 2
Influence of Silver and Copper Nanoparticles on Physiological Characteristics of Phaseolus vulgaris L. in vitro and in vivo. KMINKI 1Harir S. Mustafa, 2Asma G. Oraibi* International journal of current microbiology and applied sciences 6 (1 … , 2017 2017 Citations: 29
MOST CITED SCHOLAR PUBLICATIONS
Influence of Silver and Copper Nanoparticles on Physiological Characteristics of Phaseolus vulgaris L. in vitro and in vivo. KMINKI 1Harir S. Mustafa, 2Asma G. Oraibi* International journal of current microbiology and applied sciences 6 (1 … , 2017 2017 Citations: 29
Moringa oleifera in Vitro Culture and its Application as Anti-Diabetic in Alloxan Induced Diabetic Albino Mice (): AG Oraibi International Journal of Current Microbiology and Applied Sciences 5 (2), 43-49 , 2016 2016 Citations: 27
Induction of genetic variation for drought tolerance in two rice cultivars Amber 33 and Amber Baghdad. . KM Aurabi, A. K., Yousif, S. A. and Ibrahim Iraq J. Biotech 11 (2), 270-281 , 2012 2012 Citations: 15
Green Biosynthesis of Silver Nanoparticles Using Malva parviflora Extract for Improving a New Nutrition Formula of a Hydroponic System KHA A. G. Oraibi, H. N. Yahia Scientifica 2022 (special issue), 10 , 2022 2022 Citations: 13
Investigation the antibacterial activity of Portulaca oleracea L. tissue cultures in vitro A Oraibi, A AlShammari, R Mohsien, W Obaid Journal of Pharmaceutical Research International 18 (5), 1-7 , 2017 2017 Citations: 13
Studying the effect of anti-amylase inhibitor extracted from white kidney bean (Phaseolus vulgaris) in treat diabetes and obesity in an affected mice G Abdulwahid, Sh. and Asma Int.J. Curr. Microbiol. App. Sci 3 (10), 97-106 , 2014 2014 Citations: 10
Salmonella enterica species isolated from local foodstuff and patients suffering from foodborne illness: Surveillance, antimicrobial resistance and molecular detection ZA AlShaheeb, ZA Thabit, AG Oraibi, AA Baioumy, TG Abedelmaksoud Теория и практика переработки мяса 8 (2), 112-123 , 2023 2023 Citations: 7
Carum carvi mediated green synthesis of copper nanoparticles and its effect on Solanum lycopersicum seedlings AG Oraibi, AA Rashad, MH Ahmed J. Arid. Agric 9, 9-15 , 2023 2023 Citations: 7
Synthesis of bio-active silver nanoparticles against human lung cancer cell line (a549) with little toxicity to normal cell line (WRL68) KM Talib, AG Oraibi, GI Abass Archives of Razi Institute 78 (5), 1624 , 2023 2023 Citations: 6
Biosynthesizing of silver nanoparticles from Apium graveolens and Myrtus communis plants, detection, characterization and study their effect on genetic variation and growth of … OI Alwaan, IA Jdyea, AG Oraibi Baghdad, Iraq: Al-Iraqia University , 2021 2021 Citations: 6
Silver nanoparticles and NPKK fertilizer effects on the proline, peroxidase, and catalase enzymes in wheat WJ Atia, AG Oraibi SABRAO J. Breed. Genet 56 (6), 2405-2415 , 2024 2024 Citations: 5
INCREASING SOME FLAVONOIDS COMPOUNDS FOR ECHINACEA PURPUREA L. USING COPPER OXIDE NANOPARTICLES IN VITRO. MH Ahmed, ZS Omran, AG Oraibi Iraqi Journal of Agricultural Sciences 55 (2), 733-743 , 2024 2024 Citations: 4
Effect of green-biosynthesis aluminum nanoparticles (Al NPs) on Salmonella enterica isolated from Baghdad city ZA AlShaheeb, AG Oraibi, ZA Thabit Baghdad Science Journal 20 (5 (Suppl.)) , 2023 2023 Citations: 4
Antibacterial and Cytotoxic Activity of Mentha arvensis L. Leaves Methanolic Extract In vitro. BH Saleh, DN Al-Ugaili, AG Oraibi, RA Ibrahim Journal of Pure & Applied Microbiology 17 (2) , 2023 2023 Citations: 4
Green biosynthesis, Identification and characterization of Ag and Zn nanoparticles using Ivy (Epipremnum aureum) plant extract RIHOAG *1Hammodi H.F.2 Plant Archives 19 (2), 959-965 , 2019 2019 Citations: 4
Antibacterial activity of silver nanoparticles and Pomegranate (Punica granatum L.) extracts A Oraibi, R Mohsien, W Atea, H Yahia, L Raheem Int. J. Curr. Microbiol. App. Sci 5 (12), 131-140 , 2016 2016 Citations: 4
ANTIMICROBIAL ACTIVITIES OF GERANIUM EXTRACT AND SILVER, COPPER NANOPARTICLES AMA Assma Gatta, Mokhtar Jawad, Anas Sabah Bahjat3, Nabeel k.Al-ani* International Journal of Biological & Pharmaceutical Research. 6 (2), 84-86 , 2015 2015 Citations: 4
Investigation of growth factors and DNA markers for drought tolerance in some rice (Oryza sativa L.) genotypes AG Oraibi Ph. D. Thesis, College of biotechnology. Univ. of Al-Nahrain. Baghdad. Iraq , 2013 2013 Citations: 3
The effect of drought on physiological and biochemical characteristics of some rice genotypes. KM Aurabi, A. K., Yousif, S. A. and Ibrahim .,Iraq J. Agric. Res 1 (17), 225-232 , 2012 2012 Citations: 3
Phytoremediation of Zinc Ion Using Faba Bean Plant Vicia faba in vitro KH Alobaidi, AG Oraibi, HN Yahya, JR Al-Obaidi Indian Journal of Ecology 47 (4), 965-968 , 2020 2020 Citations: 2