Plasma metabolomic signatures in children with autism spectrum disorder and their modulation following a gluten-free modified ketogenic diet Renu Singh, Alka Shah, Nayan Jain, Harshuti Shah, Rakesh Rawal BMC Psychiatry, 2026 Gluten-free modified ketogenic diets (GF-MKD) have gained interest as adjunct nutritional interventions in autism spectrum disorder (ASD). However, evidence regarding their systemic metabolic effects in children, particularly from non-Western populations, remains limited. An untargeted plasma metabolomics analysis was performed using liquid chromatography–tandem mass spectrometry (LC–MS/MS) in 10 Indian children with ASD and 10 age- and sex-matched neurotypical controls. Multivariate and machine learning–based approaches were applied to identify metabolites distinguishing ASD from controls. Children with ASD subsequently underwent a three-month GF-MKD intervention, after which plasma metabolomic profiles and autism severity, assessed using the Childhood Autism Rating Scale (CARS), were re-evaluated. At baseline, children with ASD exhibited a distinct plasma metabolomic signature characterized by elevated L-leucine, a marked increase in coumarin (~ 6-fold), and reduced betaine levels. This metabolic profile differentiated ASD from controls with high discriminative accuracy (AUC = 0.93). Pathway enrichment analyses indicated alterations in branched-chain amino acid metabolism and one-carbon metabolic pathways. Following GF-MKD intervention, plasma levels of L-leucine and coumarin decreased by approximately 46% and 60%, respectively, while betaine levels showed a modest increase. Clinically, participants demonstrated a significant reduction in CARS scores (median decrease: 4.5 points; p < 0.05), indicating improvement in autism-related behavioural symptoms. No diet-related adverse effects were observed. Indian children with ASD display a modifiable plasma metabolomic profile involving key amino acid and methyl-donor pathways. Modulation of these metabolic disturbances following GF-MKD intervention was accompanied by behavioural improvement. These findings support the potential role of targeted dietary strategies in ASD and highlight the need for larger, randomized controlled trials to clarify underlying mechanisms and long-term clinical outcomes. Not applicable
Computational and experimental investigation of Kaempferol and Quercetin as potential inhibitors of MMP1 in oral cancer Vaidehi P. Thakore, Sai Swetha Uppalapati, Sivakumar Prasanth Kumar, Amit Mandoli, Prabhudas S. Patel, Nayan K. Jain Computational Biology and Chemistry, 2026 Oral Cancer (OC) remains the most frequently reported malignancy characterized by low survival-rate, attributing to the lack of effective therapeutic-targets. Activities of several proteases, particularly matrix metalloproteinase (MMPs), induce epidermal hyperplasia increasing susceptibility to tumorogenesis. Their dual-nature in disease, wide-substrate specificity, and compensatory machinery makes them challenging to target. MMPs inhibitors have faced consequential challenges in clinical-applications. Hence, present study aimed to identify natural inhibitors of MMPs to aid preventive measures against OC carcinogenesis and therapeutics. Current study analyzed gene-expression of MMP1, MMP3, and MMP10 in tissues of head and neck cancer and normal, suggesting MMP1 as potential target. Virtual screening was performed to identify potential natural inhibitor for MMP1 target. Molecular Dynamic simulations (MDS) supported the binding abilities of the prioritized compounds, and ADMET analysis showed promising pharmacokinetic and pharmacodynamics properties. Computational findings were validated using in-vitro analysis and MMPs gene expression was evaluated after treatment in Cal27 and NOE cell lines. The results showed significant overexpression of MMPs in tumor samples and were strongly associated with clinico-pathological characteristics. Virtual screening identified L-epicatechin, 3-O-Methylquercetin, Fisetin, Quercetin, and Kaempferol as potential MMP1 inhibitors with high binding-affinity. Further, Kaempferol and Quercetin showing stability, conformation, and intermolecular interactions through MDS were prioritized for in-vitro validation. Kaempferol and Quercetin exhibited anti-proliferative and anti-migratory effects on Cal27 cells in time- and dose-dependent manners. Impaired gene-expression of MMPs after the treatment suggested potential of these compounds in preventing tumor invasion and metastasis and highlighted them as therapeutic-agents for the treatment-strategies in OC.
Impact of CYP19A1 genetic variations on polycystic ovary syndrome: findings from a case-control study Hiral Chaudhary, Jalpa Patel, Nayan K. Jain, Sonal Panchal, Purvi Nanavati, Mala Singh, Naresh Laddha, Rushikesh Joshi F and S Science, 2025 OBJECTIVE: To study the association between CYP19A1 genetic variants and the risk of developing polycystic ovary syndrome (PCOS). The study explored the relationship between the candidate gene CYP19A1 and hyperandrogenism, as well as its interplay with obesity, in PCOS patients compared with healthy controls. DESIGN: A case-control study with genetic association analysis by tetra-primer amplification refractory mutation system polymerase chain reaction and biochemical analysis. SUBJECTS: 204 women (113 PCOS patients and 91 healthy controls) were included in the present study. EXPOSURE: CYP19A1 variants (rs700519 and rs2236722) in PCOS women. MAIN OUTCOME MEASURES: Genotypic and allelic frequencies of CYP19A1 variants (rs2236722 and rs700519) and their impact on androgen metabolism and obesity markers. RESULTS: The genotypic and allelic frequency of rs2236722 showed statistically significant differences between PCOS cases and controls. A significant association was observed under the dominant model, with an odds ratio of 0.34 (95% confidence interval, 0.16-0.66), as well as under the heterozygous model, where the odds ratio was 2.58 (95% confidence interval, 1.34-4.97). However, rs700519 did not reveal any significant association between the groups. A noticeable statistical difference was observed in the levels of total testosterone, dehydroepiandrosterone sulfate , prolactin, luteinizing hormone/follicle-stimulating hormone , Estradiol/total testosterone ratio, body mass index, and waist-to-hip ratio between the case and control groups . However, no variations in clinical variables were observed among genotypes within the PCOS group. CONCLUSION: Our study demonstrates that the CYP19A1 rs2236722 polymorphism significantly correlates with PCOS risk, although rs700519 showed no significant association. The findings suggest that altered aromatase activity linked to rs2236722 may contribute to the hyperandrogenic phenotype observed in PCOS patients. These results enhance our understanding of the genetic basis of PCOS and may have implications for personalized treatment approaches.
Desmocollin-3 and Bladder Cancer Chandreshwar P. Shukla, Nayan K. Jain, Michael A. O’Donnell, Kapil V. Vachhani, Rashmi Patel, Janki Patel, Rajiv Modi, Arpit Dheeraj, Jee Min Lee, Annah Rolig, Sanjay V. Malhotra, Bakulesh Khamar Diseases, 2025 Background: Desmocollin3, a transmembrane protein, is expressed in the basal/suprabasal layer of normal stratified epithelium. DSC3 gene expression is described in muscle-invasive bladder cancer (MIBC). DSC3-protein-expressing recurrent non-muscle-invasive bladder cancer (NMIBC) had a durable response to CADI-03, a DSC3-specific active immunotherapy. Methods: We evaluated DSC3 protein expression and its correlation with tumor-infiltrating immune cells in bladder cancer. DSC3 gene expression and its correlation with 208 immune encoding genes, treatment outcome, and survival were evaluated using the “ARRAYEXPRESS” and “TCGA” datasets. Immune genes were grouped as tumor-controlling immune genes (TCIGs) and tumor-promoting immune genes (TPIGs) as per their functions. Results & conclusions: NMIBC had higher DSC3 expression compared to MIBC. More immune genes were correlated with DSC3 in MIBC (21) compared to NMIBC (11). Amongst the TCIGs, six in NMIBC and one in MIBC had a negative correlation while two in NMIBC and nine in MIBC had a positive correlation with DSC3. Amongst the TPIGs, nine in NMIBC and five in MIBC had a negative correlation. Seven TPIGs had a positive correlation with DSC3 in MIBC and none in NMIBC. Of the T cell exhaustion markers, none were correlated with DSC3 in MIBC. Among NMIBC, CTLA4 and TIGIT were the only markers of exhaustion that demonstrated a negative correlation with DSC3. DSC3 expression was also higher in p53 mutant compared to wild p53, non-papillary MIBC compared to papillary MIBC, and in basal, squamous molecular subtype compared to luminal MIBC. MIBC with lower DSC3 expression had better outcomes (response, survival) compared to those with higher DSC3 expression.
Design and evaluation of biomass combustor and solar dryer for turmeric processing AMA Agricultural Mechanization in Asia Africa and Latin America, 2016
Ameliorative action of synthetic and herbal antioxidants on lead induced hepatotoxicity: An in vitro study Asian Journal of Pharmaceutical and Clinical Research, 2016
Drying kinetics of greengram Journal of Food Science and Technology, 1997
RECENT SCHOLAR PUBLICATIONS
Plasma metabolomic signatures in children with autism spectrum disorder and their modulation following a gluten-free modified ketogenic diet R Singh, A Shah, N Jain, H Shah, R Rawal BMC psychiatry 26 (1), 341 , 2026 2026
From pathogen effectors to plant enhancers-Harpin proteins as novel biostimulants: A review J Patoliya, K Thaker, P Kumar, N Jain, R Joshi International Journal of Biological Macromolecules, 152410 , 2026 2026
Sociodemographic and Developmental Correlates of Autism Spectrum Disorder Severity in Children from Ahmedabad, Western India R Singh, A Shah, N Jain, H Shah, B Parekh, R Rawal Nutritional Psychiatry, 100013 , 2026 2026
Functionalized drug delivery systems: Need and significance N Rohit, KLS Pallem, A Aswal, U Gupta, NK Jain Functionalized Drug Delivery Systems, 1-23 , 2026 2026 Citations: 1
Salivary metabolomic profiling and the impact of gluten-free modified ketogenic diet in children with autism spectrum disorder R Singh, A Shah, N Jain, H Shah, B Parekh, SK Patel, P Shelat, R Rawal Food and Humanity 5, 100887 , 2025 2025 Citations: 3
Biophysical characterization of Hpa1 protein from Xanthomonas orzyae J Patoliya, K Thaker, J Bajapai, D Patel, N Jain, P Kumar, R Joshi Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 141111 , 2025 2025 Citations: 1
Efficacy of Bacopa monnieri in Mitigating Lead-Induced Blood Toxicity in Swiss Albino Mice Compared to Synthetic Antioxidants F Shah, N Jain, V Muthu, S Muthu, JBA Wahid, M Shah, K Nagaraj Biological Trace Element Research, 1-12 , 2025 2025
Computational and experimental investigation of Kaempferol and Quercetin as potential inhibitors of MMP1 in oral cancer VP Thakore, SS Uppalapati, SP Kumar, A Mandoli, PS Patel, NK Jain Computational Biology and Chemistry, 108716 , 2025 2025 Citations: 2
Role of osteoporosis and diabetes on facial and long bone fracture healing under various loading conditions PV Pattar, V Yadav, N Jain, I Saha, A Nazir, P Dhir, M Mehta, J Pathi Bioinformation 21 (9), 3305 , 2025 2025
Distinct immune pathways activated by harpin proteins in Sorghum bicolor J Patoliya, K Thaker, K Rabadiya, J Patel, T Trivedi, S Patel, N Jain, ... Physiology and Molecular Biology of Plants 31 (5), 785-793 , 2025 2025 Citations: 2
Essential oil intervention: The antimicrobial and antibiofilm properties of clove oil and Eugenol against vibrio cholerae O1 L Poojara, J Prajapati, NK Jain, RM Rawal Food and Humanity 4, 100533 , 2025 2025 Citations: 6
Desmocollin-3 and Bladder Cancer SVMBK Chandreshwar P. Shukla, Nayan K. Jain, Michael A. O’Donnell, Kapil V ... Diseases 13 (131), 1-20 , 2025 2025 Citations: 1
Impact of CYP19A1 genetic variations on polycystic ovary syndrome: findings from a case-control study H Chaudhary, J Patel, NK Jain, S Panchal, P Nanavati, M Singh, ... F&S Science , 2025 2025 Citations: 7
A Review on Cutting-Edge Technology for Revitalizing and Reducing Water Pollution DPSNKJ Krishnakumar B. Vaghela Research Advances in Environment, Geography and Earth Science 4, 69-86 , 2024 2024
Investigating the interplay between AMH gene polymorphism rs10407022 and clinical indicators in polycystic ovary syndrome H Chaudhary, J Patel, NK Jain, S Panchal, N Laddha, R Joshi Human Gene 40, 201279 , 2024 2024 Citations: 6
Up-regulation of extracellular-matrix and inflammation related genes in oral squamous cell carcinoma VP Thakore, KD Patel, HH Vora, PS Patel, NK Jain Archives of oral biology 161, 105925 , 2024 2024 Citations: 19
The Role of Gastrointestinal Disorders and Nutrition in Autism Spectrum Disorder: A Comprehensive Review NJ Alka Shah, Renu Singh, Harshuti Shah, Rakesh Rawal Third Concept, 86-94 , 2024 2024
Uncovering the Interaction Interface Between Harpin (Hpa1) and Rice Aquaporin (OsPIP1;3) Through Protein–Protein Docking: An In Silico Approach RJ Jaimini Patoliya, Khushali Thaker, Khushbu Rabadiya, Dhaval Patel, Nayan ... Molecular Biotechnology 66 (01), 756-768 , 2024 2024 Citations: 20
BIOMAGNIFICATION POTENTIAL OF POLLUTANTS IN THE AQUATIC ECOSYSTEM DPSNKJ Krishnakumar B. Vaghela European Journal of Biomedical ISSN 2349-8870 European Journal of Biomedical … , 2024 2024
QT-Interval Dispersion as a Marker of Left Ventricular Hypertrophy and MACE in Patient with Essential Hypertension MS Siddiqui, D Kumar, N Jain, P Diwan 2024
MOST CITED SCHOLAR PUBLICATIONS
Development of probiotic yogurt: effect of strain combination on nutritional, rheological, organoleptic and probiotic properties R Soni, NK Jain, V Shah, J Soni, D Suthar, P Gohel Journal of Food Science and Technology 42383 (DOI 10.1007/s13197-020-04238-3 … , 2020 2020 Citations: 119
The role of polymorphism in various potential genes on polycystic ovary syndrome susceptibility and pathogenesis NKJRJ Hiral Chaudhary, Jalpa Patel Journal of Ovarian Research 14 (125), 1-21 , 2021 2021 Citations: 109
"Knowledge, attitude and practice of health professionals about probiotic use in Ahmedabad, India" NJ Richa Soni, Kamna Tank Nutrition & Food Science 48 (1), 125-135 , 2018 2018 Citations: 68
Physicochemical analysis of water from various sources and their comparative studies DHAM Devangee shukla* , Kinjal Bhadresha, Dr. N. K. Jain IOSR Journal of Environmental Science, Toxicology and food technology 5 (3 … , 2013 2013 Citations: 57
Anticancer effect of Moringa oleifera leaves extract against lung cancer cell line via induction of apoptosis K Bhadresha, V Thakore, J Brahmbhatt, V Upadhyay, N Jain, R Rawal Advances in Cancer Biology-Metastasis 6, 100072 , 2022 2022 Citations: 55
Mapping of rice-cropping pattern and cultural type using remote-sensing and ancillary data: A case study for South and Southeast Asian countries KR Manjunath, RS More, NK Jain, S Panigrahy, JS Parihar International Journal of Remote Sensing 36 (24), 6008-6030 , 2015 2015 Citations: 55
Recent trends in tropospheric NO2 over India observed by SCIAMACHY: Identification of hot spots NKJARSASJ Pallipad Atmospheric pollution research 4, 354-361 , 2013 2013 Citations: 54
Sequential Effects of Ultraviolet Radiation on the Histomorphology, Cell Density and Antioxidative Status of the Lens Epithelium—An In Vivo Study ¶ SRK Johar, UM Rawal, NK Jain, AR Vasavada Photochemistry and photobiology 78 (3), 306-311 , 2003 2003 Citations: 51
Extraction, Characterization and Application studies of red pigment of halophile Serratia marcescens KH1R KM035849 isolated from Kharaghoda soil NKJHAM J. U. Vora International Journal of Pure & Applied Bioscience 2 (6), 160-168 , 2014 2014 Citations: 47
Synergism of curcumin and cytarabine in the down regulation of multi-drug resistance genes in acute myeloid leukemia K Shah, S Mirza, U Desai, N Jain, R Rawal Anti-Cancer Agents in Medicinal Chemistry-Anti-Cancer Agents) 16 (1), 128-135 , 2016 2016 Citations: 46
Expanding the spectrum of csecretase gene mutation-associated phenotypes: two novel mutations segregating with familial hidradenitis suppurativa (acne inversa) and acne conglobate U Ratnamala, D Jhala, NK Jain, NM Saiyed, M Raveendrababu, MV Rao, ... Exp Dermatol 25 (4), 314-316 , 2016 2016 Citations: 40
Screening of efficient Arbuscular mycorrhizal fungi for Azadirachta indica under nursery condition: A step towards afforestation of semi-arid region of western India. K. Banerjee, M. H. Gadani, K. K. Shrivasta, Neelam Verma, Y. T. Jasrai, N. K ... Brazilian Journal of Microbiology 44 (2), 587-593 , 2013 2013 Citations: 40
†œBirds of Gujarat University Campus, Ahmedabad†NK Jain, SN Patel, MV Patel ZOOS'PRINT JOURNAL 20 (12), 2111-2113 , 2005 2005 Citations: 39
Derivation of rice crop calendar and evaluation of crop phenometrics and latitudinal relationship for major south and south-east Asian countries: A remote sensing approach RS More, KR Manjunath, NK Jain, S Panigrahy, JS Parihar Computers and Electronics in Agriculture 127, 336-350 , 2016 2016 Citations: 30
Association of FTO gene variant rs9939609 with polycystic ovary syndrome from Gujarat, India H Chaudhary, J Patel, NK Jain, S Panchal, N Laddha, R Joshi BMC medical genomics 16 (1), 216 , 2023 2023 Citations: 29
Design and synthesis of 1, 4-dihydropyridine derivatives as anti-cancer agent D Viradiya, S Mirza, F Shaikh, R Kakadiya, A Rathod, N Jain, R Rawal, ... Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal … , 2017 2017 Citations: 29
Curcumin targets circulating cancer stem cells by inhibiting self-renewal efficacy in non-small cell lung carcinoma S Mirza, A Vasaiya, H Vora, N Jain, R Rawal Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal … , 2017 2017 Citations: 29
Phytochemical screening, total phenolic content, antibacterial and antioxidant activity of wild edible mushroom Pleurotus ostreatus S Parihar, KD Virani, EA Pithawala, MD Shukla, SK Lahiri, NK Jain, ... International Research Journal of Pharmacy 6 (1), 65-69 , 2015 2015 Citations: 29
Anticancer and cytotoxic potential of turmeric (Curcuma longa), neem (Azadirachta indica), tulasi (Ocimum sanctum) and ginger (Zingiber officinale) extracts on HeLa cell line. NKJ Devangee P. Shukla, Krupa P. Shah, Rakesh M. Rawal Int. J. Life Sci. Scientific. Res. 2 (4), 309-315 , 2016 2016 Citations: 25
Evidence for circulating cancer stem-like cells and epithelial–mesenchymal transition phenotype in the pleurospheres derived from lung adenocarcinoma using liquid biopsy S Mirza, N Jain, R Rawal Tumor Biology 39 (3), 1-10 , 2017 2017 Citations: 22