Komariah
@trisakti.ac.id
Dentistry
Universitas Trisakti
RESEARCH INTERESTS
Chitosan
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Komariah Komariah, Pretty Trisfilha, Rahman Wahyudi, Nada Erica, Didi Nugroho, Yessy Ariesanti, and Sarat Kumar Swain
Universitas Gadjah Mada
During phagocytosis, phagocyte cells discharge reactive oxygen species referred to as respiratory bursts, inducing a rise in pro‐oxidants and subjecting the cell to oxidative stress. Such stress is a biological mechanism related to an imbalance in pro‐oxidant/antioxidant homeostasis, which generates toxic reactive oxygen. Encapsulation is a coating process to improve the stability of bioactive compounds from lemongrass extract. Therefore, this study aims to determine the encapsulation activity of lemongrass leaf extract with chitosan X. gideon (LEChXg) to reduce the oxidative stress of fibroblasts. The research used the human dermal fibroblast (HDF) cell line, comprising negative and positive controls and use of LEChXg 100, 200, 300, 400, and 500 µg/mL. HDF cell migration was evaluated by employing the scratch wound healing method and the wound closure was oberseved at 0, 2, 4, 6, and 24 h intervals. The cell proliferation was observed at 24, 48, and 72 h using CCK‐8 at a 450 nm wavelength. The results showed that the observations at 0, 2, and 4 h did not demonstrate any significant difference on the cell migration (p > 0.05) among the groups. However, the wound closure at 4 and 6 h showed a significant difference (p < 0.05) with LEChXg 300 µg/mL. Despite the lack of any significant variation observed up to 24 h, fibroblast subjected to the stressor did not achieve complete closure. The groups treated with LEChXg were more stable in maintaining fibroblast proliferation up to the end of the observation than those with stressors at 24, 48, and 72 h. Fibroblast induced with a stressor was also more stable in maintaining migration and proliferation in groups receiving LEChXg 300 µg/mL.
Eric Ngezahayo, Komariah Komariah, and Mohammad Masykuri
IOP Publishing
Abstract Orthosiphon aristatus (Java Tea) is a traditional and economically valuable medicinal plant with different pharmaceutical activities for preventing various diseases. Air temperature is one of the environmental elements that have an impact on plant growth and herbal production. The study aimed to assess how java tea plants respond to high air temperatures and to learn if soil with compost fertilizer may have a substantial impact on plant growth. The research was arranged using a completely randomized design, with air temperature as the main factor with three replicates for each treatment. Plastic Pots of java tea plants were maintained in the greenhouse (control) for three months and in the growth chamber day/night temperatures of 25/25°C, 30/25°C, 35/25°C, 40/25°C, 45/25°C and 50/25°C. Plants placed in a growth chamber showed significantly affected by temperature by comparing treatments in the greenhouse (control). Java tea plants kept at 30/25°C and 35/25°C had considerably higher plant height, leaf numbers, fresh and dry weight values than other treatments, whereas the 40°C treatment had the greatest growth decline, reduced leaf number, dry weight, and fresh weight. Moreover, all plants subjected to 45/25°C and 50°C/25°C in the growth chamber suffered more severely in all traits up to mortality. Therefore,30/25°C treatment would be more valuable for high marketability due to increased plant height, leaf number, and dry mass compared to other treatments.
Nada Fitria, Del Afriadi Bustami, Komariah Komariah, and Indra Kusuma
Editora Cubo
Objective : The purpose of this research is to assess the antioxidant activity of lemongrass leaves extract in terms of lowering ROS generation and its effect on the viability and proliferation of fibroblasts under oxidative stress. Material and Methods : The antioxidant activity was measured using the DPPH method and the ROS assay was carried out by fluorescent H2DCFDA staining. Viability and proliferation assays were performed using the Cell Counting Kit-8 (CCK-8) and was read at 450 nm using microplate reader. The groups were divided into 8, namely fibroblasts without treatment (comparison group), fibroblast induced by H 2 O 2 (negative control), fibroblast with H 2 O 2 then treated with ascorbic acid (positive control), and fibroblast with H 2 O 2 then treated with lemongrass leaves extract at various concentrations (10, 20, 30, 40, and 50 ppm). Results : The results showed that the antioxidant activity of lemongrass leaves extract had an IC 50 value of 64.17 ppm. ROS production were reduced by the LgLE of all concentrations if compared with negative control (p=0.819). LgLE can maintained the fibroblast viability with 10 ppm of LgLE was the most optimum concentration (p<0.05). LgLE can induced the proliferation of fibroblast, with the most effective was at 24 h of observation (p<0.05). Conclusion : Lemongrass leaves extract has a strong antioxidant activity that can reduce oxidative stress and increase the viability and proliferation of fibroblasts with the optimum concentration is at 10 ppm.
Muhamad Khoiru Zaki, Keigo Noda, Kengo Ito, Komariah Komariah, Sumani Sumani, and Masateru Senge
MDPI AG
We have rectified the list of ethnic groups in Java, which should include Madurese and the often neglected, forgotten, and stigmatized Chinese Indonesians [...]
H Zaenudin and K S Komariah
IOP Publishing
Abstract It is a fact that the condition of the Citarum River is full of problems. The problems are about plastic waste, pollution of household waste, and industrial waste. To deal with the problems, the President intended to revitalize the Citarum River by establishing the Republic of Indonesia Presidential Regulation Number 15/2018 concerning the Acceleration of Pollution Control and Damage to the Citarum River watershed. The program is intently broadcasted through various mass media to raising people’s awareness of controlling pollution and damage of Citarum River watershed. It is the vital function of media to inform environmental insights and activate community actions (proper disposal of industrial and domestic wastes). By informing actual information, mass media can contribute to structuring social understanding and social-behaviour improvement. The present study employed a case study approach. The researchers focused on the deep-longitudinal aspects of Citarum Harum Program. The analysis was following stages; observation, data collection, analysis, and dissemination. The analysis was mainly based on interviews to reliable individuals ranging from mass media practitioner, government officials, activists, and scholars. The results shows that printed media, television, and radio have social responsibility to educate the society about the river protection. In addition, policies in several media are considered to be consistent in disseminating the messages and ideas as they also create innovations in structural, social, and cultural approaches.
Florencia Livia, Rosalina Tjandrawinata, Carolina Damayanti Marpaung, Deviyanti Pratiwi, and Komariah Komariah
Trans Tech Publications, Ltd.
The purpose of this study was to evaluate the effect of adding horn beetle nano chitosan (NCH) to Glass Ionomer Cement (GIC) liquid on surface roughness. The evaluation was done before and after the addition of NCH and from the length of time soaking in artificial saliva. The disc sample with a diameter of 6 mm and a height of 3 mm of total 40 samples were divided into four groups: the GIC control group, GIC modified NCH 0.5%, 1%, and 2%. Each sample was immersed for 24 hours and seven days in artificial saliva. Surface roughness before and after immersion was measured using the Surtronic S-100 Series Surface Roughness Tester. Data analysis was performed using a one-way ANOVA test to determine the effect of adding horn beetle NCH and paired t-test to determine the effect of immersion time on the surface roughness of GIC. Although the highest surface roughness values were found in sample GIC modified NCH 1% (2.51±0.86 for 24 hours) and in sample GIC modified NCH 2% (2.64±0.84 for 7 days), there was no significant differences for both the surface roughness with the addition of NCH (p > 0.05) and the length of immersed time (p > 0.05). As the conclusion, there is no effect of horn beetle nano chitosan to GIC surface roughness. However, the addition of horn beetle NCH presented rougher surfaces after immersion. These experiments can help predict the performance of these materials under clinical conditions.
Sasqia Faadillah Andikoputri, Komariah Komariah, M. Orliando Roeslan, Dewi Ranggaini, and Del Afriadi Bustami
Walter de Gruyter GmbH
Abstract World-wide, Indonesia is ranked 17th in oral cancers, with deaths reaching 2.326 cases. Of the oral cancers, 90% are squamous cell carcinoma (HSC-3). Unfortunately, conventional cancer therapy still has many ill side effects. Therefore, pharmacologists have looked for natural ingredients to prevent the growth of oral cancer cells. One source is Cymbopogon citratus leaf. Research shows that the active compound of C. citratus leaf is a chemopreventive, doing so by increasing the production of re-active oxygen species (ROS) to induce apoptosis in cancer cells. The active compound of C. citratus leaf has low stability and solubility, so it is necessary to use an encapsulation matrix such as chitosan, and modify it into smaller particles to increase its effectiveness. Purpose is determining the effect of nano chitosan encapsulation of C. citratus leaf ethanol extract (NCECC) on the reactive oxygen species of HSC-3 tongue cancer cells. This study is divided into ten groups – without treatment, doxorubicin (positive control), hydrogen peroxide, nano chitosan and C. citratus leaf extract groups, and five groups of NCECC treatment – concentrations of 100%, 75%, 50%, 25%, and 12.5%, respectively. The 100% NCECC group showed the highest ROS concentration (p<0.05), compared to 75%, 50%, 25%, 12.5% NCECC groups, and the 100% NCECC was higher than the positive control group. NCECC is effective in inducing oxidative stress on HSC-3 through increased production of ROS. Moreover, the higher the encapsulation concentration given, the greater the increase in ROS production.
Komariah Komariah, Wasmen Manalu, Bambang Kiranadi, Adi Winarto, Ekowati Handharyani, and M. Orliando Roeslan
The Korean Society of Toxicology
Valproic acid (VPA) plays a role in histone modifications that eventually inhibit the activity of histone deacetylase (HDAC), and will affect the expressions of genes Pdx1, Nkx6.1, and Ngn3 during pancreatic organogenesis. This experiment was designed to study the effect of VPA exposure in pregnant rats on the activity of HDAC that controls the expression of genes regulating the development of beta cells in the pancreas to synthesize and secrete insulin. This study used 30 pregnant Sprague-Dawley rats, divided into 4 groups, as follows: (1) a control group of pregnant rats without VPA administration, (2) pregnant rats administered with 250 mg VPA on day 10 of pregnancy, (3) pregnant rats administered with 250 mg VPA on day 13 of pregnancy, and (4) pregnant rats administered with 250 mg VPA on day 16 of pregnancy. Eighty-four newborn rats born to control rats and rats administered with VPA on days 10, 13, and 16 of pregnancy were used to measure serum glucose, insulin, DNA, RNA, and ratio of RNA/DNA concentrations in the pancreas and to observe the microscopical condition of the pancreas at the ages of 4 to 32 weeks postpartum with 4-week intervals. The results showed that at the age of 32 weeks, the offspring of pregnant rats administered with 250 mg VPA on days 10, 13, and 16 of pregnancy had higher serum glucose concentrations and lower serum insulin concentrations, followed by decreased concentrations of RNA, and the ratio of RNA/DNA in the pancreas. Microscopical observations showed that the pancreas of the rats born to pregnant rats administered with VPA during pregnancy had low immunoreaction to insulin. The exposure of pregnant rats to VPA during pregnancy disturbs organogenesis of the pancreas of the embryos that eventually disturb the insulin production in the beta cells indicated by the decreased insulin secretion during postnatal life.