Olga Shydlovska
@knutd.edu.ua
DEPARTMENT OF BIOTECHNOLOGY, LEATHER AND FUR/Faculty of Chemical and Biopharmaceutical Technologies
Kyiv National University of Technologies and Design
RESEARCH INTERESTS
biotechnology, virology, microbiology, genetics, molecular biology, immunology, agrotechnology, nanotechnology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Olga Iungin, Olga Shydlovska, Olena Moshynets, Volodymyr Vasylenko, Marina Sidorenko, Saulius Mickevičius, and Geert Potters
Mark Allen Group
Metal-based nanoparticles (MNPs) are promoted as effective compounds in the treatment of bacterial infections and as possible alternatives to antibiotics. These MNPs are known to affect a broad spectrum of microorganisms using a multitude of strategies, including the induction of reactive oxygen species and interaction with the inner structures of the bacterial cells. The aim of this review was to summarise the latest studies about the effect of metal-based nanoparticles on pathogenic bacterial biofilm formed in wounds, using the examples of Gram-positive bacterium Staphylococcus aureus and Gram-negative bacterium Pseudomonas aeruginosa, as well as provide an overview of possible clinical applications.
Yugin Kharchenko, Liudmyla Lastovetska, Valeriia Maslak, Marina Sidorenko, Volodymyr Vasylenko, and Olga Shydlovska
MDPI AG
Green synthesis of nanoparticles is a widely researched and popular direction in the development of nanotechnology. It is a simple, cheap and effective method for obtaining nanoparticles with interesting biological properties. In light of the development of antibiotic resistance to important clinical strains of bacteria, this method was used in the present study to obtain silver nanoparticles with antibacterial activity. The aim of this study was to synthesise silver nanoparticles with antibacterial action by yeast in a process known as “green synthesis”. We are also considering the prospect of using silver nanoparticles as an antibacterial substance for drug development. The production of nanoparticles was confirmed by UV spectroscopy. Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 test strains and Staphylococcus aureus 1536 and Klebsiella pneumoniae 520 clinical isolates were used to study the antibacterial effect. The effect of synthesised nanoparticles on the metabolic activity of bacterial cells and their ability to adhere, as well as the minimum inhibitory concentrations (MICs) of synthesised nanoparticles for each of the strains, were determined. Following UV spectroscopy, the nanoparticles obtained were found to have a pronounced peak in optical absorption at 400 nm, corresponding to the plasmon resonance of silver nanoparticles, and demonstrated a high antibacterial effect against all the strains studied.
Olga Shydlovska and Yuliia Khmelnytska
INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania
Dairy products are important in human diet and nutrition. That is why dairy production is critical not only economically, but also socially and medically. In recent decades, dairy production has had problems with disturbances in fermentation processes caused by bacteriophage contamination. It is important to note that every year there are new reports about newly discovered bacteriophages that disrupt fermentation processes in the production of kefir, yogurt, and various types of cheese. Lactococcus lactis strains are of particular importance in dairy technology, as they are used for the production of various yogurts and cheeses. The study of the spectrum of bacteriophages infecting this strain can help to monitor the evolutionary changes of viruses and the horizontal transfer of genes. In this paper, an analysis of phages infecting L. lactis was carried out. Most bacteriophages belong to the Siphoviridae and Podoviridae families. Moreover, the authors analyzed approaches that can be used to reduce bacteriophage contamination in the production of dairy products. It has been shown that the use of disinfectants, such as ethanol on sodium hypochlorite, can reduce the titer of bacteriophages and protect products from the development of viral infection. It is also possible to use membrane filtration with UV irradiation. Moreover, all these approaches can be combined to achieve the most effective result.
Olga Shydlovska and Yevhen Kharchenko
INCDTP - Leather and Footwear Research Institute (ICPI), Bucharest, Romania
Green synthesis of metal nanoparticles is a very promising area of research. Silver nanoparticles are the most interesting type of nanoparticle in nanotechnology because they have varied properties, such as antibacterial, antioxidant, and antibiofilm forming properties. This review aims to establish which of the most common approaches for the biological synthesis of nanoparticles is the best. In this work, the methods of synthesis of silver nanoparticles using plant extracts, bacteria, and yeast are considered. Each of these methods has its advantages and disadvantages. The most common method of synthesis of silver nanoparticles is the method using plant extracts, however, stabilizing substances from plant extracts have their own direct biological activity, which can be both enhanced and suppressed by silver nanoparticles. Green synthesis of nanoparticles thanks to microorganisms makes it possible to use a wide range of bacterial strains, but it is important to remember of the pathogenicity of the strains and their danger to humans. From this perspective, the use of yeast for the synthesis of silver nanoparticles is the most promising method, as it allows obtaining a large amount of nanomaterial. The synthesis, thanks to yeast method, allows us to control the size and shape of the nanoparticles. Nanoparticles obtained from yeast lysates have effective antibacterial and antifilm-forming activity.
Olga A. Shydlovska and Nadiya M. Zholobak
IEEE
In the research, we were used normal cells that were received ex vivo, and transfected cell lines to establish the toxicity of non-stabilized and citrate-stabilized nanoparticles of cerium dioxide and to study their influence on the nitrite ion production. As a result, there was established the most effective concentrations of different type cerium dioxide nanoparticles and was showed the different response of normal and tumor cell lines.
Olga Shydlovska, Nadiya Zholobak, Svitlana Dybkova, Sergej Osinsky, Larissa Bubnovskaya, Oleksandr Yelenich, Sergii Solopan, and Anatolii Belous
Walter de Gruyter GmbH
AbstractIn the last decade, ferromagnetic nanoparticles that are able to be heated under an AMF (alternating magnetic field) have gained considerable interest in the field of nanotechnology. The current study explores the peculiarity of the synthesis and the properties of Fe
O. Khylko, N. Rusinchuk, O. Shydlovska, M. Lokshyn, V. Lozovski, V. Lysenko, A. Marynin, A. Shcherbakov, M. Spivak, and N. Zholobak
American Scientific Publishers
Nadezhda M. Zholobak, Alla P. Mironenko, Alexander B. Shcherbakov, Olga A. Shydlovska, Mykola Ya. Spivak, Larysa V. Radchenko, Andrey I. Marinin, Olga S. Ivanova, Alexander E. Baranchikov, and Vladimir K. Ivanov
Elsevier BV
We have demonstrated the influence of cerium dioxide nanoparticles on the immunogenicity of the influenza vaccine on an example of liquid split inactivated Vaxigrip vaccine. Antibody titers were analyzed using the hemagglutination inhibition (HI) assay. Seroprotection, seroconversion, the geometric mean titers (GMTs) and the factor increase (FI) in the GMTs were calculated. The effect of nano-ceria surface stabilizer on the enhancement of immunogenicity was shown. The vaccine modified by citrate-stabilized nano-ceria, in contrast to a non-modified Vaxigrip vaccine, did not provide an adequate level of seroprotection, and seroconversion after vaccination was 66.7% on days 49-63 for virus strain А(H1N1) and 100% on day 49 for virus strain B/Yamagata. For the low immunogenic influenza B virus, the rise in antibody titers (GMT/IF) was 24.38/3.28 after the first injection and 50.40/6.79 on day 49. For the vaccine modified by non-stabilized nano-ceria, for all virus strains under study, on day 63, upon immunization notable levels of seroprotection, seroconversion and GMT/IF were registered (higher than for the non-modified Vaxigrip vaccine). The successful attempt to modify the influenza vaccine demonstrates the possible ways of increasing the specific activity of vaccines using nano-ceria.
M. Lokshyn, V. Lozovski, V. Lysenko, Yu. Ushenin, N. Rusinchuk, O. Shydlovska, M. Spivak, and N. Zholobak
American Scientific Publishers