Investigation of the Inhibitory Effects of ChemicaCompounds on Eco-Efficiency: an Analysis Based on the Activity of Bacillus licheniformis Kambiz Najafi, Nahid Haghnazari, Kambiz Davari, Fatemeh Keshavarzi Polish Journal of Environmental Studies, 2025 The penicillinase enzyme plays an important role in local ecological efficiency and resistance to antibiotic agents. <i>Bacillus licheniformis</i>, the causative agent of septicemia, has a high penicillinase activity. The main goal of this study was to identify selective inhibitors for clinical applications. To achieve this, the inhibitory effects of four chemical compounds on the activity of the Bacillus licheniformis (<i>B. licheniformis</i>) penicillinase enzyme were evaluated. <i>B. licheniformis</i> PTCC 1320 (NCIB6346) was cultured in tryptic soy broth (TSB) (30 g/L) liquid culture medium and was adjusted to 0.5 McFarland turbidity standard (4-hr culture). Mueller-Hinton broth (MHB) was used to adjust the turbidity. Then, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of benzylpenicillin antibiotic (penicillin G) were determined. Then, a concentration of 8 mg/ml of citrate was chosen and consistently added to all the tubes (serial tubes). Enzyme inhibition by Citra and other inhibitors was evaluated by measuring MIC and MBC in the presence of each compound separately. Finally, the results were confirmed by molecular modeling data. The MIC and MBC levels of benzylpenicillin were determined to be 2 and 4 μg/mL, respectively. However, in the presence of citrate, the MIC and MBC values of benzylpenicillin were found to be 0.125 and 0.25 μg/mL, respectively. Moreover, molecular docking results showed that the active site of penicillinase had an anionic cavity where amino acid anions (Thr 235, Arg 24, Ser 70, Ser 130, Tyr 135, Asn 170, and Asn 104) were present and stabilized the intermediates formed in this site by inducing electrostatic interactions. Citrate could competitively attach to this site. The other three compounds (diethyl malate, malic anhydride, and malic acid) showed similar binding patterns to citrate, but were smaller in size and had fewer active groups: their interactions were less strong and weaker than those of citrate can enhance ecological efficiency. The results show that citrate is the best inhibitory compound for <i>B. licheniformis</i> penicillinase as well as for promoting ecological construction.
Develop a Fe2O3/Cu/Ag Nanocomposite for Removing Tetracycline (TC) Pollutants from Aqueous Solutions Rasoul Hosseini, Fatemeh Keshavarzi, Nahid Haghnazari, Changiz Karami Iranian Journal of Chemistry and Chemical Engineering, 2024 In this study, iron oxide, silver, and copper were used to create a Fe2O3/Cu/Ag nanocomposite to remove tetracycline (TC) as pollutants from aqueous solutions. Various techniques were employed to analyze the composite structure, including X-Ray Diffraction (XRD) crystallography, Transmission Electron Microscopy (TEM), scanning electron microscopy (SEM), Energy-Dispersive X-ray (EDS) spectroscopy, mapping, and Fourier Transform InfraRed (FT-IR). The impact of environmental pH, reaction time, temperature, and the maximum amount of TC on removal efficiency was investigated. The findings showed that an optimal pH of 5, 150 mg of Fe2O3/Cu/Ag, and 20-minute reaction time at room temperature resulted in 98.53% removal of tetracycline. In conclusion, Fe2O3/Cu/Ag adsorbents show promise for effectively eliminating tetracycline from aqueous environments.
The Investigation of the Association Between the Bcl-2 3’-UTR rs1564483 Polymorphism and miR-296-3p in the Development of Breast and Gastric Cancers Mehrnoush Azadeh Jouneghani, Fatemeh Keshavarzi, Nahid Haghnazari, Zahra Hooshmandi, Sabrieh Amini Clinical Medicine Insights Oncology, 2023 Background: B-cell leukemia/lymphoma 2 ( Bcl-2) gene regulates carcinogenesis by inhibiting apoptosis. This study evaluated the association of Bcl-2 3′-untranslated regions (3′ UTR) rs1564483 polymorphism and miR-296-3p with the development of breast and gastric cancers. Methods: A microarray analysis was performed on the Genomic Spatial Event (GSE)29431 and GSE161533 datasets for breast and gastric cancers. Blood samples were taken from 222 (111 patients and 111 controls) and 210 (84 patients and 126 controls) individuals for breast and gastric cancers, respectively. Genomic DNA was extracted from the blood samples and genotyping was performed using real-time polymerase chain reaction (RT-PCR), followed by examining the high-temperature melting curve. Statistical analysis was conducted to examine the potential correlation between the rs1564483 polymorphism and the risk of breast and gastric cancers concerning pathological characteristics. Results: The results of the microarray showed that the Bcl-2 gene was up-regulated in gastric cancer (logFC [log fold change]: 0.65, adjusted P < .05). Clinical outcome showed no notable relationship between the rs1564483 polymorphism and breast cancer risk; however, for gastric cancer, it identified a large difference between healthy controls and patients for an allelic frequency of rs1564483 ( P ⩽ .001). Moreover, an assay of different models (dominant, recessive, and co-dominant) showed a significant association between the AG genotype between control and gastric cases (Pearson chi-square test, P = .046). In addition, the prevalence of the AG genotype was greater in persons under the age of 45 and in patients with H. pylori infection ( P ⩽ .001). The AG genotype was not related to smoking, although the AA genotype was associated with increased cancer incidence in smokers ( P ⩽ .001). Conclusions: In silico studies and calculations of the ΔG binding of micro ribonucleic acid (miRNA) hsa-miR-296-3p to the mutant and wild alleles of the rs15644833 single nucleotide polymorphism (SNP) have revealed that Bcl-2 mRNA expression in gastric cancer decreases, thus confirming the tumor suppressor role of the Bcl-2 gene.
The Association of rs5745687 Polymorphism Located at HGF Gene with Risk of Gastric and Breast Cancer in the Helicobacter Positive Patients of Isfahan Population Mehrnoush Azadeh Jouneghani, Fatemeh Keshavarzi, Nahid Haghnazari, Sabrieh Amini, Zahra Hooshmandi International Journal of Cancer Management, 2022 Background: Hepatocyte growth factor (HGF) protein regulates cell growth, motility, and morphogenesis in a variety of cells and tissues by binding to the HGF receptor. The rs5745687 SNPs in the introns of the HGF gene could affect the splicing and expression of HGF mRNA. Objectives: In this study, the genotype frequency of rs5745687 in breast cancer (BC) and gastric cancer (GC) (positive helicobacter) patients has been investigated and compared with the healthy controls in the Isfahan population. Methods: Firstly, initial bioinformatics studies were done. Then, according to the results, bioinformatics high-resolution melt (HRM) and real-time PCR were recruited to determine genotypes rs5745678 for 432 participants in the case-control analysis (84 GC with 126 healthy control samples, as well as 111 BC cases with 111 normal controls). The conditional logistic regression model was used to measure odds ratios (OR) and 95% confidence intervals (CI) to produce these cancers based on genotype frequency. Results: The homozygote genotype of the mutant (G) allele of rs5745678 has a significant association with the lower risk of gastric cancer (P-value < 0.0001) and this allele can increase the risk of GC in a co-dominant model (OR: 5.541, P-value < 0.0001). Also, the rs5745678 SNP had a significant association with the clinicopathological features (age, smoking, Helicobacter Pylori infection) in GC patients. Conclusions: The presence of a single G allele in rs5745678 heterozygote (AG/AA) and co-dominant (AG/AA+GG) models could significantly impact GC pathogenicity in different ways. There was no significant correlation between the rs5745678 polymorphism and BC (P-value: 0.671) in the studied sample size.
Determination of phenobarbital in real sample using carbon quantum dots modified with tungsten as a fluorescent nanoprobe Hossein Javadzad, Nahid Haghnazari, Changiz Karami Journal of Materials Science Materials in Electronics, 2022 In this study, a sensitive and selective fluorescence nanoprobe has been designed based on carbon quantum dots modified by tungsten (WCQDs) for the detection of phenobarbital. Tungsten WCQDs were synthesized via a hydrothermal method using tungsten nitrate and glucose as a carbon source. The fluorescence signal of WCQDs was selectively increased by phenobarbital. The fluorescence increasing signal was applied for phenobarbital sensing at the pH = 8 without the interference of other materials. After optimizing the factors affecting the sensor’s response, a linear range between 25 and 500 µM with a detection limit of 5 µM was obtained. The sensor’s capability in the real-sample analysis was investigated by phenobarbital determination in human blood plasma samples.
Cytotoxic and antiproliferative effects of methanolic extract of lavandula angustifolia on mcf-7 breast cancer cell line Scientific Journal of Kurdistan University of Medical Sciences, 2021
The effect of NaOH and KOH on the characterization of mesoporous AlOOH nanostructures in the hydrothermal route Journal of the Mexican Chemical Society, 2014
