HARISCHANDRA NAIK R
@uasraichur.edu.in
Department of Agricultural Entomology
University of Agricultural Sciences, Raichur
I have subject expertise in Pesticide Toxicology, Pesticide Residue, Food Contaminants and Food Quality Analysis; and Insecticide Resistance and Management. I have ten years of experience in teaching, research, and extension activities at UAS, Raichur. I have been involved in developmental activities such as the establishment of Pesticide Residue and Food Quality Analysis Laboratory and obtaining the NABL accreditation. Involved in the establishment of a pesticide formulation testing laboratory.
EDUCATION
Ph.D in Agricultural Entomology-2012
RESEARCH INTERESTS
Pesticide Toxicology
Pesticide Residue Analysis
Insecticide Resistance and Management
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Saroja Narsing Rao, G. Monika Kumari, D. Srividya, H. S. Anil, M. Lakshmikanth, Harishchandra Naik, and A. Prabhuraj
Springer Science and Business Media LLC
Harischandra R Naik, M. S Pallavi, Rahul Chawan, M Bheemanna, Anand Naik, and M Paramasivam
Food Analytical Methods Springer Science and Business Media LLC
A simple, sensitive and reproducible analytical method for quantitative determination of indoxacarb residues in pigeonpea green pod, pigeonpea dry grain and soil was developed and validated using LC-MS/MS. The developed methodology was linear having correlation coefficients (R 2 ) value of 0.999. The LOD and LOQ were 0.0015 and 0.005 μg g −1 , respectively. QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) technique involved for extraction of indoxacarb residues with 1% ethyl acetate in acetonitrile provided acceptable recoveries in the range of 83.80–99.01% at 0.005, 0.025 and 0.050 μg g −1 spiking level with precision RSD of less than 1.20 %. Initial deposit on green pods following two application (15 days interval between application) of indoxacarb 14.5% SC at 60 and 120 g a.i.ha −1 was 0.583 and 1.342 μg g −1 and dissipated to 0.005 and 0.006 μg g −1 on 7 and 10 days after application, respectively. Half-life and safe waiting period (SWP) were ranged from 1.13 to 1.23 days and 3.23 to 5.03 days for the dose 60 and 120 g a.i.ha −1 in pigeonpea green pods, respectively. Hazard index (HI) value was more than 1 in green pods drawn on 5th and 7th day after application in recommended (60 g a.i.ha −1 ) and double the recommended dose (120 g a.i.ha −1 ), respectively.
Rachappa V., Shivayogiyappa ., Harischandra N., and Suhas Yelshetty
Agricultural Research Communication Center
An experiment was conducted at Agriculture Research Station, Kalaburagi, Karnataka, India during kharif, 2015-16 to understand the effect of differential population of leafhoppers, on growth and yield parameters of pigeonpea under field conditions with seven level of population load. Maximum of 26.16 hoppers/3 leaves were found at 12 pairs release plot which was at par with 10 pairs (22.21) and 8 pairs (24.96) released which were statistically significant. The average hopper count @ 4 and 6 pairs released plots were 13.14 and 16.15 per 3 leaves respectively and were at par among themselves and significantly higher than 2 pairs release and lower than 8 pairs release plots. This was a clear indication that the differential population of leafhopper had impact in reduction of growth and yield parameters with. Reduction in yield to the tune of 3.12, 5.29, 10.05, 17.99, 24.63 and 31.91 % at 2, 4, 6, 8, 10 and 12 pairs released plots, respectively. From the present investigation it was clear that the 5.59 to 5.77 leafhoppers aggregated in top leaf at 90 to 120 days aged crop of medium duration variety will cause 10 per cent reduction in yield.
R Kaur, M Subbarayalu, R Jagadeesan, G J Daglish, M K Nayak, H R Naik, S Ramasamy, C Subramanian, P R Ebert, and D I Schlipalius
Springer Science and Business Media LLC
Phosphine (PH3) fumigation is the primary method worldwide for controlling insect pests of stored commodities. Over-reliance on phosphine, however, has led to the emergence of strong resistance. Detailed genetic studies previously identified two loci, rph1 and rph2, that interact synergistically to create a strong resistance phenotype. We compared the genetics of phosphine resistance in strains of Rhyzopertha dominica and Tribolium castaneum from India and Australia, countries having similar pest species but widely differing in pest management practices. Sequencing analysis of the rph2 locus, dihydrolipoamide dehydrogenase (dld), identified two structurally equivalent variants, Proline49>Serine (P49S) in one R. dominica strain and P45S in three strains of T. castaneum from India. These variants of the DLD protein likely affect FAD cofactor interaction with the enzyme. A survey of insects from storage facilities across southern India revealed that the P45/49S variant is distributed throughout the region at very high frequencies, in up to 94% of R. dominica and 97% of T. castaneum in the state of Tamil Nadu. The abundance of the P45/49S variant in insect populations contrasted sharply with the evolutionary record in which the variant was absent from eukaryotic DLD sequences. This suggests that the variant is unlikely to provide a strong selective advantage in the absence of phosphine fumigation.
M. Paramasivam, S. Chandrasekaran, R. Harischandra Naik, P. Karthik, P. Thangachamy, and C. A. Mahalingam
Informa UK Limited
A simple and sensitive QuEChERS method for the determination of imidacloprid residues in mulberry leaf was developed using high performance liquid chromatography (HPLC). This method was then evaluated for the residual level and dissipation rate of imidacloprid in mulberry leaves. A maximum initial deposits of imidacloprid on mulberry leaves was found to be 3.047 µg g−1 and 6.490 µg g−1 following two application rates of imidacloprid 17.8 SL at 0.6 and 1.2 mL L−1. The calculated half-life of imidacloprid in mulberry leaves was at recommended dose 3.81 d with a dissipation rate of 76% over 7 d; whereas, in double, the dose was 4.93 days with a dissipation rate of 84% over 10 d.