KHALID SUHAIL A. AL-AZZAWI

@nahrainuniv.edu.iq

Researcher & Assistant Professor / Forensic DNA Center for Research &Training
High Institute of Forensic Science/AL-Nahrain University

KHALID SUHAIL A. AL-AZZAWI


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https://researchid.co/khalid1st

RESEARCH, TEACHING, or OTHER INTERESTS

Physiology, Molecular Biology, Cell Biology, Biotechnology
8

Scopus Publications

79

Scholar Citations

4

Scholar h-index

2

Scholar i10-index

Scopus Publications

  • Determining the Role of KRAS in Patients with Different Demographic Profiles Diagnosed with Diabetes Mellitus: A Case-Control Study
    Noora A. Hadi, Rehab S. Ramadhan, Khalid Suhail A. AlAzzawi, Rebah N. Algafari, Sura S. Talib, Rawaa A. Khalaf
    Obm Genetics, 2026
    Diabetes mellitus (DM) is widely spread among populations. About 3 of 10 people show the symptoms of this disease. Many factors may cause this illness in both types (type 1 and type 2 DM), mainly attributed to insufficient insulin production in type 1 DM or developing insulin resistance in type 2 DM. Genes that control specific biochemical pathways involving glucose metabolism can interfere with the manifestation of this disease when they undergo genetic changes like KRAS. Investigating the role of KRAS in DM. About 124 patients with both types of DM and of different genders and ages participated in this study. In addition to demographic characteristics, biochemical and diabetic status were determined for all participants. DNA was isolated from all patients, and the KRAS gene was amplified using specific primers designed for this purpose and thoroughly analyzed to determine any genetic change within its sequence. The results showed the spread of DM, especially type 2, among undereducated participants, and a significant increase in fasting blood sugar, uric acid, and SGOUT. Molecular analysis of the KRAS gene identified two repeated single-nucleotide variations (SNVs): rs1475732807 and rs1306089842. Both SNVs affected the 3’ UTR of the KRAS gene, causing an alteration in gene expression, which consequently affected the severity of DM. Diabetes mellitus is widely spread among different societies, especially in undereducated groups, due to unhealthy diets or genetic factors. Some genes, like KRAS, can interfere with the manifestation of DM, especially related to glucose metabolism. SNVs in the 3’ UTR of KRAS may dramatically boost the symptoms in some patients.
  • The Possible Role of Progesterone Receptors in Prostate Cancer Incidences in the Iraqi Population
    Jaleel Ibrahim Asaad, Khalid S. A. Alazzawi, Sara S. Rasheed, Rebah N. Algafari, Rehab S. Ramadhan, Marwah Amer Qamandar, Sura S. Talib, Rawnaq Z. Fadhil
    Frontiers in Bioscience Scholar, 2024
    Background: Prostate cancer (PCa) is one of the leading diseases causing mortality. It comes in the third rank of common cancer types. It is considered extremely a complicated cancer type since it occurs in highly steroid-responsive and dependent tissues. Many factors are considered to play an important role in the disease progression of PCa, with some functioning at the molecular level. Methodology: After applying the exclusion criteria, 200 patients who underwent proctectomy were included in this study. Following receiving patient consent, blood samples were withdrawn from patients, DNA was extracted, and precise polymerase chain reaction (PCR) amplification was conducted using specifically designed primers. The resulting amplicons were sequenced and analyzed. Results: The progesterone receptor B (PGRB) DNA from patients showed four distinctive single-nucleotide polymorphisms (SNPs) at sites 11:101128812, 11:101128924, 11:101128949, and 11:101128986, which altered the amino acid sequences to Y>N, A>D, T>I, and C>R, respectively, compared to control. These SNPs resided in sensitive sites that either affected the control elements or promoted alterations in the protein configuration. This DNA change diminished the PGR gene function and promoted an imbalance in the encoded PGR protein structure and expression. Conclusions: Many factors may play a role in PCa manifestation, with steroids and progesterone initially noted as factors. Many studies have dealt with the hormonal effect on PCa; however, few have ultimately determined the molecular impact on disease progression. The presence of pathogenic SNPs in the enhancing region of the gene may impact the expression level of PGR. High or low expression levels may negatively affect gene function, which can be considered a reliable factor in prostate tumorigenesis.
  • In Vitro Effects of Acetaminophen Drug(Paracetamol) on Microscopic Human Sperm Parameters
    Rana R Al. Saadi, Ban Thabit Al-Ani, Khalid S. A. Alazzawi
    Aip Conference Proceedings, 2022
    Paracetamol, also recognized as acetaminophen (CH3CONH.C6H4), is a drug used to relief pain and fever. It is usually used to relief mild to moderate pain . Forty semen samples were taken from forty infertile patients. After semen analysis assessment, The samples were classified into 2 aliquots group, Pre - activation and Post-Activation group. The Post-Activation group was divided into three subgroups: First subgroup G1 (control group) containing semen sample with in vitro Flashing activation medium. Second subgroup G2 containing semen sample with in vitro Flashing activation medium supplemented by Paracetamol drug powder (0.0226 g/l). Third subgroup G3 containing semen sample with in vitro Flashing activation medium supplemented with Paracetamol drug powder (0.0453 g/l). Direct swim up and centrifugation technique was used for sperm activation in vitro . Sperm parameters were assessed and data were analyzed statistically pre- and post-activation in G1,G2 and G3. The results showed a significant increase in the percentage of sperm motility (%) in G1 and G2 groups (P<0.05) in post-activation when compared with pre-activation. However, there was no significant difference in the post-activation sperm total motility (%) between the G1 and G2 groups. Also there is significant difference (P<0.05) of normal sperm morphology observed between Pre- and Post-Activation using Flashing medium supplemented with Paracetamol drug and there is non-significantly different (P>0.05) of post activation of subgroup G1 and G2 and G3. There were significant different (P<0.05) sperm concentration seen between Pre- and Post-activation using Flashing medium supplemented with Paracetamol Drug while there is non-significantly different (P>0.05) of post activation groups compare with each other G1,G2 and G3. The results of this clinical research indicated paracetamol as antipyretic, analgesic drug have no significant (in vitro) effect on sperm motility, concentration, morphology in post activated semen samples. So this drug not preferred to use for increase IVF technique successful rate.
  • Benefit effect of ethanolic extract of Bay leaves (Laura nobilis) on blood sugar level in adult diabetic rats induced by alloxan monohydrate
    Salah M.M. Al Chalabi, Duha Mysire Majeed, Ahmed Abdulmunem Jasim, Khalid Suhail Al-Azzawi
    Annals of Tropical Medicine and Public Health, 2020
    In the present study, the effect of Bay leave ethanolic extract on blood glucose, insulin level, lipid profile , liver and kidney function and body weight in type 2 diabetic rats were investigated. Forty albino male rats(250-300) grams were divided into four groups (10 rats for each), first group treated with distilled water and served as control, second group treated with 200 mg\\kg of Laura nobilis alcoholic extract, third group served as positive diabetic and the fourth group was served as diabetic and treated with 200 mg\\kg b. w of alcoholic extract of Laurus nobilis, all groups were treated orally, the following results were evaluated in diabetic rats, fasting blood glucose, triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL), very low density lipoprotein (VLDL),alanine aminotransferase (ALT),aspartate aminotransferase (AST) ,alkaline phosphatase (ALP) , B. urea and s. creatinine levels were significantly increased while high density lipoprotein (HDL) and body weight significantly decreased as compared to control rats, after 30 days of treating by200 mg\\kg b.w alcoholic extract of Bay leave there was significant decrease in fasting blood glucose and significant increase in fasting insulin level, also there was significant decrease in TG, TC, LDL, VLDL, ALT, AST, ALP, B. urea and s. creatinine level, while HDL and body weight increased in diabetic groups compared to control not treated groups. Key word: Diabetes, alcoholic extract, cholesterol and creatinine How to cite this article: Al Chalabi SMM, Majeed DM, et al (2020): Benefit effect of ethanolic extract of Bay leaves (Laura nobilis) on blood sugar level in adult diabetic rats induced by alloxan monohydrate, Ann Trop Med & Public Health; 23(S16): SP231608. DOI: O.2020.231608 http://doi.org/10.36295/ASR
  • Usage of soybean (Glycine max) diluent in storage of semen of bulls at 5oC
    Plant Archives, 2020
  • SPERMATOZOA PARAMETERS STUDIES AND MALE REPRODUCTIVE HORMONES AFFECTED BY MAGNETIC WATER IN ALBINO MICE
    Biochemical and Cellular Archives, 2020
  • Evaluation of the effects of different solvents extracts of tribulus terrestris obtained via ultrasonic extraction on human asthenozoosperm activation in vitro
    Journal of Global Pharma Technology, 2019
  • The effect of mechanical vibration on human sperm activity in vitro
    Ghassan Thabet Saeed, Khalid Suhail A. Al-Azzawi, Hydar Saadi Hassan Al-Wasti
    Biomedical and Pharmacology Journal, 2018
    It is well known that sperm is a unique cell in that it has a function to be done by itself outside the body and this function is essential for species' continuity thus sperm by its power and intact structure has to reach the ova and perform the fertilization and this journey is affected by the chemical and physical factors that might increase or decrease its ability to move or fertilize or even to survive. The aim of this study is to find the effect of vibration that is a vigorous movement with high frequency for 20min on whole seminal fluid samples as an external physical factor. 40 fresh seminal fluid samples were selected. 1ml of each semen samples was placed in the bottom of conical tube; the tube was exposure to vibration waves by using a special shaker designed for this purpose for 20 min. This shaker consist of a M540 DC motor equipped with PWM controller to control the rotational speed from 5-2400 rpm. Semen analysis was done before and after subject vibration. A significant increase (P&lt;0.05) was found in percentage of sperm active directed motility (grade A) with a non-significant increase in sluggish motility and a non-significant decrease in percentage of immotile sperms percentage. No significant changes were founded regarded sperm morphology and count. It was concluded that vibrating seminal sample for 20min increases the overall sperms activity with significant increase in percentage of highly active directed sperms.

RECENT SCHOLAR PUBLICATIONS

  • Determining the Role of KRAS in Patients with Different Demographic Profiles Diagnosed with Diabetes Mellitus: A Case-Control Study
    RAK Noora A. Hadi , Rehab S. Ramadhan , Khalid Suhail A. AlAzzawi , Rebah N ...
    OBM Genetics 10 (1), 1-13 , 2026
    2026
  • MicroRNA-Mediated Regulation of Granulosa Cell Apoptosis: A Review
    KSAA Rana R. Al-Saadi, Ban Thabit Al. Ani
    Research Medical Journal (RMJ) 49 (1), 19 , 2025
    2025
  • The Possible Role of Progesterone Receptors in Prostate Cancer Incidences in the Iraqi Population
    KSAASSR Jaleel Ibrahim Asaad
    Frontiers in Bioscience-Scholar 16 (3), 1-10 , 2024
    2024
  • Removal of Colloidal Suspension through Coagulation – Flocculation Process In Water Purification – A Review
    KA AbdulSalam T.Dawood, Khalid S.A.Alazzawi, Israa H.Salman
    JOURNAL OF BIOTECHNOLOGY RESEARCH CENTER 18 (2), 38-64 , 2024
    2024
    Citations: 5
  • In vitro effects of acetaminophen drug(Paracetamol) on microscopic human sperm parameters
    BTAAKSAA Rana R Al. Saadi
    1st Samarra International Conference for Pure and Applied Sciences … , 2022
    2022
  • Positive effect of Allium sativum and Citrus aurantifolium plants on glucose level in diabetic rats using Alloxan
    SMM Al Chalabi, KS AL-Azzawi
    Annals of the Romanian Society for Cell Biology 25 (4), 6787–6795 , 2021
    2021
    Citations: 1
  • Study the physiological and immunological effects of pumpkin extract on wound healing in diabetic rats
    SM Al Chalabi, AI Sail, KS Al-Azzawi
    Annals of Tropical Medicine & Public Health 24 (4), 362-370 , 2021
    2021
    Citations: 3
  • Benefit effect of ethanolic extract of Bay leaves (Laura nobilis) on blood sugar level in adult diabetic rats induced by alloxan monohydrate
    SMM Al Chalabi, DM Majeed, AA Jasim, KS Al-Azzawi
    Annals of Tropical Medicine and Health 23 (16), 231-608 , 2020
    2020
    Citations: 27
  • SPERMATOZOA PARAMETERS STUDIES AND MALE REPRODUCTIVE HORMONES AFFECTED BY MAGNETIC WATER IN ALBINO MICE
    JIAKAR Khalid S. Al-Azzawi
    Biochemical and Cellular Archives 20 (1), 1543-1548 , 2020
    2020
    Citations: 2
  • USAGE OF SOYBEAN (GLYCINE MAX) DILUENT IN STORAGE OF SEMEN OF BULLS AT 5OC
    JI Asaad, HI AL-Ahmed, KS AL-Azzawi, NI Said, RAH Mahood
    International Journal of Plant Research 20 (1), 2904-2908 , 2020
    2020
  • Evaluation of the effects of different solvents extracts of Tribulus Terrestris obtained via ultrasonic extraction on human asthenozoosperm activation in vitro
    RAS Hikmat Fakhri Wassef , Khalid Al Azzawi
    Journal of Global Pharma Technology 11 (5), 120-127 , 2019
    2019
  • The Effect of Mechanical Vibration on Human Sperm Activity in Vitro
    KSAAAHSHAW Ghassan Thabet Saeed
    Biomedical & Pharmacology Journal 11 (3), 1617-1621 , 2018
    2018
    Citations: 8
  • A Comparison Between the Effects of Global Sperm Washing® and FertiCult Flushing TM Media on Certain Sperm Function Parameters of Asthenozoospermic Men
    KSAA Zena Muzher Hussein and Ban T. Al-Ani
    Journal of Biotechnology Research Center 11 (2), 37-41 , 2017
    2017
    Citations: 1
  • HAIR DISTRIBUTION ON THE DORSUM OF THE HUMAN HAND PHALANGES IN COMPARISON WITH SEMINAL FLUID PARAMETERS
    KAAMSHFWMS Mohammad Oda Selman (FIBMS)
    International Journal of Advanced Research (IJAR) 5 (9), 1225-1230 , 2017
    2017
  • The Antidiabetic Activity of Curry Leaves “Murraya Koenigii” on the Glucose Levels, Kidneys, and Islets of Langerhans of Rats with Streptozotocin Induced Diabetes
    KSA Imad M Al-Ani, Rahajoe I Santosa, Muhammad H Yankuzo, Anil K Saxena
    Makara J. Health Res. 21 (2), 54-60 , 2017
    2017
    Citations: 31
  • The Benefit of Semen Cryostorage on Certain Sperm Function Parameters Following in Vitro Activation
    KSA AL-Azzawi
    Diyala Journal of Medicine 4 (1), 5 , 2013
    2013
    Citations: 1
  • Effect of incubation time on certain sperm function parameters following in vitro activation test by FertiCult medium
    NKF Maha K.A., Khalid Suhail. A.AL-Azzawi, Rana A.A.
    Iraqi Journal of Embryos and Infertility Researches 2 (3), 4 , 2012
    2012
  • تنشيط النطف البربخية في الفئران باستخدام مصل دم النساء الفاعل المضاف الى اوساط زرعية مختلفة ‎
    خالد سهيل عبود العزاوي ‎
    المؤتمر العلمي العاشر لكلية العلوم / الجامعة المستنصرية 21 (6), 8 , 2010 ‎
    2010
  • IN VITRO ACTIVATION OF EPIDIDYMAL SPERMATOZOA AFTER EIGHT WEEKS OF VASECTOMY IN MICE
    IMAKSAAA M.T.Ridha-Albarazanchi,S.S. Al-Dujaily, S.A. Alanssari
    Fourth Asian Symposium on Animal Biotechnology 4 (269), 3 , 1998
    1998

MOST CITED SCHOLAR PUBLICATIONS

  • The Antidiabetic Activity of Curry Leaves “Murraya Koenigii” on the Glucose Levels, Kidneys, and Islets of Langerhans of Rats with Streptozotocin Induced Diabetes
    KSA Imad M Al-Ani, Rahajoe I Santosa, Muhammad H Yankuzo, Anil K Saxena
    Makara J. Health Res. 21 (2), 54-60 , 2017
    2017
    Citations: 31
  • Benefit effect of ethanolic extract of Bay leaves (Laura nobilis) on blood sugar level in adult diabetic rats induced by alloxan monohydrate
    SMM Al Chalabi, DM Majeed, AA Jasim, KS Al-Azzawi
    Annals of Tropical Medicine and Health 23 (16), 231-608 , 2020
    2020
    Citations: 27
  • The Effect of Mechanical Vibration on Human Sperm Activity in Vitro
    KSAAAHSHAW Ghassan Thabet Saeed
    Biomedical & Pharmacology Journal 11 (3), 1617-1621 , 2018
    2018
    Citations: 8
  • Removal of Colloidal Suspension through Coagulation – Flocculation Process In Water Purification – A Review
    KA AbdulSalam T.Dawood, Khalid S.A.Alazzawi, Israa H.Salman
    JOURNAL OF BIOTECHNOLOGY RESEARCH CENTER 18 (2), 38-64 , 2024
    2024
    Citations: 5
  • Study the physiological and immunological effects of pumpkin extract on wound healing in diabetic rats
    SM Al Chalabi, AI Sail, KS Al-Azzawi
    Annals of Tropical Medicine & Public Health 24 (4), 362-370 , 2021
    2021
    Citations: 3
  • SPERMATOZOA PARAMETERS STUDIES AND MALE REPRODUCTIVE HORMONES AFFECTED BY MAGNETIC WATER IN ALBINO MICE
    JIAKAR Khalid S. Al-Azzawi
    Biochemical and Cellular Archives 20 (1), 1543-1548 , 2020
    2020
    Citations: 2
  • Positive effect of Allium sativum and Citrus aurantifolium plants on glucose level in diabetic rats using Alloxan
    SMM Al Chalabi, KS AL-Azzawi
    Annals of the Romanian Society for Cell Biology 25 (4), 6787–6795 , 2021
    2021
    Citations: 1
  • A Comparison Between the Effects of Global Sperm Washing® and FertiCult Flushing TM Media on Certain Sperm Function Parameters of Asthenozoospermic Men
    KSAA Zena Muzher Hussein and Ban T. Al-Ani
    Journal of Biotechnology Research Center 11 (2), 37-41 , 2017
    2017
    Citations: 1
  • The Benefit of Semen Cryostorage on Certain Sperm Function Parameters Following in Vitro Activation
    KSA AL-Azzawi
    Diyala Journal of Medicine 4 (1), 5 , 2013
    2013
    Citations: 1
  • Determining the Role of KRAS in Patients with Different Demographic Profiles Diagnosed with Diabetes Mellitus: A Case-Control Study
    RAK Noora A. Hadi , Rehab S. Ramadhan , Khalid Suhail A. AlAzzawi , Rebah N ...
    OBM Genetics 10 (1), 1-13 , 2026
    2026
  • MicroRNA-Mediated Regulation of Granulosa Cell Apoptosis: A Review
    KSAA Rana R. Al-Saadi, Ban Thabit Al. Ani
    Research Medical Journal (RMJ) 49 (1), 19 , 2025
    2025
  • The Possible Role of Progesterone Receptors in Prostate Cancer Incidences in the Iraqi Population
    KSAASSR Jaleel Ibrahim Asaad
    Frontiers in Bioscience-Scholar 16 (3), 1-10 , 2024
    2024
  • In vitro effects of acetaminophen drug(Paracetamol) on microscopic human sperm parameters
    BTAAKSAA Rana R Al. Saadi
    1st Samarra International Conference for Pure and Applied Sciences … , 2022
    2022
  • USAGE OF SOYBEAN (GLYCINE MAX) DILUENT IN STORAGE OF SEMEN OF BULLS AT 5OC
    JI Asaad, HI AL-Ahmed, KS AL-Azzawi, NI Said, RAH Mahood
    International Journal of Plant Research 20 (1), 2904-2908 , 2020
    2020
  • Evaluation of the effects of different solvents extracts of Tribulus Terrestris obtained via ultrasonic extraction on human asthenozoosperm activation in vitro
    RAS Hikmat Fakhri Wassef , Khalid Al Azzawi
    Journal of Global Pharma Technology 11 (5), 120-127 , 2019
    2019
  • HAIR DISTRIBUTION ON THE DORSUM OF THE HUMAN HAND PHALANGES IN COMPARISON WITH SEMINAL FLUID PARAMETERS
    KAAMSHFWMS Mohammad Oda Selman (FIBMS)
    International Journal of Advanced Research (IJAR) 5 (9), 1225-1230 , 2017
    2017
  • Effect of incubation time on certain sperm function parameters following in vitro activation test by FertiCult medium
    NKF Maha K.A., Khalid Suhail. A.AL-Azzawi, Rana A.A.
    Iraqi Journal of Embryos and Infertility Researches 2 (3), 4 , 2012
    2012
  • تنشيط النطف البربخية في الفئران باستخدام مصل دم النساء الفاعل المضاف الى اوساط زرعية مختلفة ‎
    خالد سهيل عبود العزاوي ‎
    المؤتمر العلمي العاشر لكلية العلوم / الجامعة المستنصرية 21 (6), 8 , 2010 ‎
    2010
  • IN VITRO ACTIVATION OF EPIDIDYMAL SPERMATOZOA AFTER EIGHT WEEKS OF VASECTOMY IN MICE
    IMAKSAAA M.T.Ridha-Albarazanchi,S.S. Al-Dujaily, S.A. Alanssari
    Fourth Asian Symposium on Animal Biotechnology 4 (269), 3 , 1998
    1998

Publications

Determining the Role of KRAS in Patients with Different Demographic Profiles Diagnosed with Diabetes Mellitus: A Case-Control Study