Vyshnava Satyanarayana Swamy
@skuniversity.ac.in
Senior Research Fellow (ICMR)
Sri Krishnadevaraya University
Working on Quantum dots based rare cell capture platform, looking for potential collberations in microbial research
RESEARCH INTERESTS
Nanobiotechnology and Applied Microbiology
Scopus Publications
Scopus Publications
Sudhakar Chekuri, Satyanarayana Swamy Vyshnava, Swarupa Lakshmi Somisetti, Sai Bindu Karamthote Cheniya, Chakradhar Gandu, and Roja Rani Anupalli
Springer Science and Business Media LLC
Satyanarayana Swamy Vyshnava and Muralidhara Rao Dowlathabad
Springer Science and Business Media LLC
Satyanarayana Swamy Vyshnava, Gayathri Pandluru, Kanderi Dileep Kumar, Shiva Prasad Panjala, Swathi Banapuram, Kameshpandian Paramasivam, Kothamunireddy Varalakshmi Devi, Roja Rani Anupalli, and Muralidhara Rao Dowlatabad
Springer Science and Business Media LLC
AbstractCancer cell heterogeneity (CCH) is crucial in understanding cancer progression and metastasis. The CCH is one of the stumbling blocks in modern medicine's therapeutics and diagnostics . An in-vitro model of co-culture systems of MCF-7, HeLa, HEK-293, with THP-1 cells showed the occurrence of EpCAM positive (EpCAM+) and EpCAM negative (EpCAM−) heterogenetic cancer cell types labeled with the Quantum Dot antibody conjugates (QDAb). This in-vitro model study could provide insights into the role of rare cancer cells manifestation and their heterogeneity in metastatic progression and risk for severe infections in these patients. We successfully report the presence of CCH based on the fluorescence ratios of the co-cultured cancer cells when treated with the QDAb. These short-term mimic co-cultures give a compelling and quite associated model for assessing early treatment responses in various cancers.
Satyanarayana Swamy Vyshnava, Gayathri Pandluru, Kanderi Dileep Kumar, Shiva Prasad Panjala, Kameshpandian Paramasivam, Swathi Banapuram, Roja Rani Anupalli, and Muralidhara Rao Dowlatabad
Wiley
Satyanarayana Swamy Vyshnava, Dileep Kumar Kanderi, and Muralidhara Rao Dowlathabad
Elsevier BV
Physico-chemical mobility of cells in three dimensions is dependent on the development of filipodia, which is the fundamental instinct for survival and other cellular functions in live cells. Specifically, our present research paper describes the synthesis of 3-Mercaptopropoinc acid (MPA) capped CdSe/ZnS quantum dots (QDs), which are biocompatible and utilized for cellular bioimaging applications. Using the pancreatic cell lines BXCP3 cells, we successfully demonstrated the applicability of MPA-capped QDs for intercellular filopodia imaging. Employing these QDs, we examined the dynamics of filopodia formation in real-time along the Z-axis by using confocal laser microscopy.
Spike proteins are a functional component in the viral infections of the SARS-CoV-2 virus that binds to the ACE2 receptors of the human cells. The naturally derived drugs like hesperidin show a higher affinity to avoiding the binding of the spike protein. Inhibition of the spike protein study represents the development of a new drug for SARS disease. Along with the hesperidin and its analogs have been found effective medicine to control SARS-CoV-2; the entire drug bank database was screened for strong analogous remedy like compounds as hesperidin. Virtual Screening and docking studies were intended for these molecules against spike protein with auto dock virtual screening tool and auto dock vena. The docking outcome showed that the compounds hesperitin, silibilin, and dihydromyricetin were having the highest binding energies, like -8 and -6.2. The current study indicates that the lead molecules have to be evaluated to improve prospective drug molecules.
Satyanarayana Swamy Vyshnava, Gayathri Pandluru, Dileep Kumar Kanderi, Shiva Prasad Panjala, Swathi Banapuram, Kameshpandian Paramasivam, Roja Rani Anupalli, Rajashekar Reddy Bontha, and Muralidhara Rao Dowlatabad
Springer Science and Business Media LLC
In this study, we described an easy and one-pot synthesis technique for high-quality CdZnS/ZnS core/shell quantum dots, which exhibit greater fluorescence at λ450 nm with steady quantum yields in both organic and aqueous solvents. At lower concentrations, they showed a decrease in cytotoxicity, which is more suitable for the biocompatible applications. Further, we successfully demonstrated the fluorescence imaging and sorting through the surface display of the quantum dots in MCF-7 and HELA cell lines using confocal microscopy and flow cytometer studies.
Satyanarayana Swamy Vyshnava, Dileep Kumar Kanderi, Shiva Prasad Panjala, Kamesh Pandian, Rajasekhar Reddy Bontha, Praveen Kumar Reddy Goukanapalle, and Babajan Banaganapalli
Springer Science and Business Media LLC
AbstractStudies were undertaken to examine the mechanism of mediation of silver nanoparticles in inhibiting biofilm formation by Pseudomonas aeruginosa through LuxI/LuxR system of signal transduction. This study includes the basic signaling transduction mechanism LasR, QscR, RhlR, and Vfr signaling model systems. The arbitrary homology models built with the I-TASSER server were evaluated and validated with the Qmean web server. Based on the Z-score and the relative square mean distance (RMSD) values, the structures were validated. The interaction results of the nanoparticle with the rigid docking proved the requirement of minimal energy for the inhibition of the protein active site by the silver nanoparticle. This principle docking experiment suggests that the biofilm formation in Gram-negative bacteria can be inhibited by the silver nanoparticles at the signal transduction level.
Graphical abstractSystematic outline of present study; Stage one provides the data sampling and generation of pdb systems to conform the structure of bacterial signal sytems like LasR/LasI; RhlR/RhrI; QscR/QscI; VfrR/VfrI. Stage two involves docking of silver nanoparticles with Bacterial signal protein strucutres which are listed in Stage one. The Final Stage involves in understanding the development of appropriate mechanism behind the biofilm inhibition by silver nanoparticles.