Haliatur Rahma
@unand.ac.id
Faculty of Agriculture
Andalas University
EDUCATION
Doctor
RESEARCH INTERESTS
Plant Protection
FUTURE PROJECTS
Biocontrol of plant pathogens
Applications Invited
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Haliatur Rahma, Trizelia Trizelia, Martinius Martinius, Gita Flawerina, and Yolma Hendra
AIP Publishing
Trizelia Trizelia, Haliatur Rahma, Martinius Martinius, Yoga Mulya Gusrisan, and Dini Puspita Yanti
AIP Publishing
H Rahma, J Trisno, Martinius, and S D Shafira
IOP Publishing
Abstract Xanthomans oryzae pv. oryzae (Xoo) is a pathogen that causes bacterial leaf blight in rice plants.Biological control using microorganisms, one of which incorporates actinobacteria, is one of the most extensively used control techniques. This work aimed to find actinobacteria isolates from rice that can inhibit Xoo growth in vitro. A Completely Randomized Design (CRD) was employed in this experiment, with 14 treatments and three replications. There are 13 isolates used: Act-Hr 49, Act-Hr 21, Act-Hr 24, Act-Pha 4, Act-Mn2, Act-LB3, Act Pha 2.3, Act Pha 3.4, Act-Pha 3.3, Act-Hr 47, Act Pha 3.5, Act-Krj 21, Act-SK2 and control (Xoo without Actinobacteria treatment). The ability of actinobacterial isolates to reduce Xoo growth in vitro and the enzyme activity of each isolate were measured. The study revealed that three Actinobacteria isolates, Act-LB3, Act-Mn2, and Act-Pha4, could potentially suppress Xoo bacteria’s growth with inhibition indices ranging from 12 mm to 15 mm Act-LB3 isolate produced catalase enzyme, while Act-Pha4 isolate produced both catalase and protease enzyme. The results indicate that actinobacteria from the rice rhizosphere could produce antibacterial metabolites and enzyme activity.
TRIZELIA TRIZELIA, HALIATUR RAHMA, and MY SYAHRAWATI
UNS Solo
Abstract. Trizelia, Rahma H, Syahrawati M. 2023. Diversity of endophytic fungi of rice plants in Padang City, Indonesia, entomopathogenic to brown planthopper (Nilaparvata lugens). Biodiversitas 24: 2384-2392. Various endophytic fungi can live in plant tissues for all or part of their life cycle without causing damage or disease in their host. Several genera of endophytic fungi have been reported to be entomopathogenic and can be used to control insect pests. This study aimed to determine the diversity of endophytic fungi from rice stems that were entomopathogenic against Nilaparvata lugens (Stål, 1854) or Brown Planthopper (BPH). Endophytic fungi were isolated from rice stems of IR42 and Cisokan varieties grown in Padang City, West Sumatra Indonesia. Isolated fungi were first evaluated for their pathogenicity against Tenebrio molitor larvae. Subsequently high pathogenicity showed isolates were tested on nymphs and adults of BPH. The results showed that the entomopathogenic fungi isolated from IR 42 and Cisokan were the members of genus Aspergillus, Trichoderma, and Beauveria. The entomopathogenic fungi from IR 42 were more diverse than Cisokan, and Beauveria sp. was the most common isolate and had a better ability to kill the BPH. The highest mortality of BPH was found in PIR 1.3 isolate, but the mortality was still relatively low (53.33% nymphs and 34.99% adults). Further study is needed to increase the effectiveness of these endophytic fungi against BPH directly or indirectly by inducing resistance.
H Rahma, Nurbailis, and N Kristina
IOP Publishing
Abstract Rhizobacteria is an environmentally friendly and sustainable biocontrol agent, used as an alternative in the management of plant pathogens. Furthermore, their suppression mechanism often varies, as some are capable of producing antibiotic compounds, competing with, and increasing plant growth, although t some forms possess multiple control. This study, therefore, aims to determine the activity of rhizobacterial antibiosis against Curvularia lunata in vitro. The experiment was conducted at the Laboratory of Biological Control, Department of Pests and Plant Diseases, Faculty of Andalas University. In addition, the procedure required using an experimental method, which was carried out in a Completely Randomized Design. The antagonistic rhizobacterial selection of C. lunata was carried out using a dual culture test, on a total of 7 rhizobacteria, and 4 were selected, encompassing Stenotrophomonas malthopilia KJKB5.4, Stenotrophomonas pavanii LMTSA5.4, Bacillus cereus AJ34 and Alcaligenes faecalis AJ14 as potential candidates in the control of C. lunata, suppression effectiveness that was above 50%. In addition, it was also established that all four isolates the propensity to produce chitinase enzymes.
R Noveriza, T L Mardiningsih, O Trisilawati, H Rahma, and Zulkarnaen
IOP Publishing
Abstract Nanobiopesticide citronella (Nano-BC) at 1% concentration was effectively reduced the intensity of mosaic disease and yield loss ranging from 23 to 43%. The mixed formulation of Serratia marsescens AR1 and Pseudomonas fluorescens LPK1-9 with cow manure (15 kg/tree) and 0.1% nanobiopesticide citronella were able to reduce 20 to 25% the intensity of vascular streak dieback disease (VSD). The combination between biofertilizer and Nano-BC is assumed to be able to reduce the intensity of patchouli mosaic virus and its insect vector and increase the resistance of patchouli plants to the disease. The objective of this study was to obtain a dose of biofertilizer (0, 100, 200, 300 g) and 1% Nano-BC that effectively controlling patchouli mosaic disease and their vectors in the field. Research was conducted on patchouli var Sidikalang at Laing Experimental Garden, Solok from January to December 2018. Application of 1% Nano-BC and 300 g biofertilizer per plant showed as the best combination to reduce the incidence and intensity of patchouli mosaic disease in the field. In addition, the highest content of P2O5 was 39.00 ppm available in the soil, in which the efficacy rate ranged from 9.37 to 12.47%.
Haliatur Rahma, NURBAILIS, and NILA KRISTINA
UNS Solo
Abstract. Rahma H, Nurbailis, Kristina N. 2019. Characterization and potential of plant growth-promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae. Biodiversitas 20: 3654-3661. Xanthomonas oryzae pv. oryzae (Xoo), a major limiting factor in rice production, and the use of resistant Xoo varieties have failed to control the bacterial pathogens as well as increased yield. It is due to the diversity in pathotypes, caused by environmental factors, the nature of resistant variety used, and gene mutation. The aims of this study were to select rhizobacterial strains with the potential of suppressing Xoo growth and promoting the growth of rice seedlings. This experiment was conducted in a completely randomized design (CRD) using seven rhizobacterial isolates selected through a dual culture test, with four replications. There were four isolates that potential in inhibiting the growth of Xoo, namely KJKB5.4, LMTSA5.4, Bacillus cereus AJ34, and Alcaligenes faecalis AJ14, with inhibition diameters greater than 11.50 mm. Rhizobacterial supernatant of 4 potential isolates has a zone of inhibition ranging from 12.25 to 24.00 mm. Four potential isolates were also able to solubilize phosphate, produce indole acetic acid (IAA) growth hormone, and siderophore, as well as enhance the growth of rice seedlings. Based on the nucleic acid sequencing of LMTSA5.4, KJKB5.4, and RK12 isolates were identified as Stenotrophomonas malthopilia strain LMG 958 (99.13%) accession NR 119220.1, Stenotrophomonas pavanii strain LMG 25348 (95.84%) accession NR 118008.1 and Ochrobactrum ciceri strain ca-34 (92.91%) accession NR115819.1.
Nurbailis Nurbailis, AKMAL DJAMAAN, HALIATUR RAHMA, and YENNY LISWARNI
UNS Solo
Abstract. Nurbailis, Djamaan A, Rahma H, Liswarni Y. 2019. Potential of culture filtrate from Trichoderma spp. as biofungicide to Colletotrichum gloeosporioides causing anthracnose disease in chili. Biodiversitas 20: 2915-2920. Trichoderma spp. have the potential to be used for controlling the airborne pathogenic fungi such as C. gloeosporioides. The purpose of this study was to evaluate the antifungal activity of the culture filtrate of five isolates of Trichoderma spp. (T. harzianum, T. viride, T. koningii, Trichoderma PP2, Trichoderma PP3) against C. gloeosporoides causing anthracnose disease in chili. Culture filtrate of Trichoderma spp. was produced from single culture and dual culture techniques. The design was a Completely Randomized Design with six treatments and four replications. The treatments were culture filtrate from T. harzianum, T. viride, T. koningii, Trichoderma PP2, Trichoderma PP3, and negative control (without culture filtrate from Trichoderma spp.). Parameters observed were: the diameter of the colony, colony coverage, conidial germination, and conidial density. The results of this research showed all the culture filtrate from Trichoderma spp. produced by single and dual culture techniques can inhibit the growth of C. gloeosporioides. The culture filtrate from Trichoderma PP2 and T. koningii were the most potential in inhibiting the growth, conidial density, and conidial germination of C. gloeosporioides.
R Noveriza, J Trisno, H Rahma, S Yuliani, Reflin, and Martinius
IOP Publishing
The disease of Vascular streak dieback (VSD) is a deadly disease of cocoa plants, because it attacks the vascular tissue of cocoa at growing point of the plant. In West Sumatra the disease was first reported in 2015 with an incidence of disease range 58.82% - 100% and an intensity of disease range 24.29% - 44.7%. The purpose of this study was to examine the effectiveness of dosage application of oil formula and nano emulsion of citronella formula against Vascular streak dieback (VSD) disease on cocoa plants in West Sumatra (in Padang Pariaman District and Limapuluh Kota District). The results showed that the percentage of VSD disease attacks in both testing sites was 100%. The oil and nano emulsion of citronella formulas can reduce the intensity of VSD disease on cocoa plants in West Sumatra, particularly in Padang Pariaman District and Limapuluh Kota District. The reduction of VSD intensity in Padang Pariaman district ranged from 8.32 to 21.13%; while in Limapuluh Kota district ranged from 4.33 to 11.80%. The nano emulsion of citronella formulation is effective to suppress the intensity of VSD disease on cocoa plants at doses 0.1% (≥ 30% of effectiveness level).
Haliatur Rahma, Aprizal Zainal, and Suryati .
Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Lampung
Isolation and selection of rhizobacteria potentially as biocontrol agents against Pantoea stewartii subsp. stewartii causing stewart’s wilt disease in maize. Group of bacteria that colonize plant roots were known as Plant Growth Promoting Rhizobacteria (PGPR), in addition to capable of suppressing the development of pathogens also has the ability to improve plant health. This study aims to isolate rhizobacteria from rhizosphere of maize in West Sumatera. Rhizobacteria were characterized and tested for its ability to suppress the bacteria Pantoea stewartii subsp. stewartii causes stewart wilt’s disease on maize. In this research 15 isolates of rizobacteria potential as biological agent were found, i.e. 6 isolates of the fluorescens bacteria group, 5 isolates of non fluorescens bacteria group and 4 isolates of heat-resistant bacteria group.
Haliatur Rahma, Aprizal Zainal, Memen Suharman, Meity S Sinaga, and Giyanto .
Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Lampung
Potential of endophytic bacteria to control stewart wilt disease (Pantoea stewartii subsp. stewartii) in maize. The purpose of this study was to explore endophytic bacteria from seedling, maize roots and grass roots as well as to test the ability of endophytic bacteria which could potentially suppress stewart wilt disease development in maize. Characterization of endophytic bacteria as biocontrol agents including: do not induce HR on tobacco, synthesize IAA, dissolve phosphate, produce siderophores, and antibiotic to Pantoea stewartii subsp. stewartii (Pnss). The results of research shoed 17 isolates of endophytic bacteria potentially as candidate biocontrol agents. Nine isolates were able to produce IAA, siderofores and phosphatase; two isolates produce IAA and phosphatase; six isolates produce IAA. Six isolates ie: AR1, AJ34, AJ15, AJ19, and AJ14 AN6, can increase maize plant resistance and suppress stewart wilt disease severity with a range of 48.95-55.60%.
Haliatur Rahma, Meity S Sinaga, Memen Surahman, and Giyanto Giyanto
Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Lampung
Disease incidence of Stewart’s wilt on the seed and response of several maize varieties to Pantoea stewartii subp. stewartii. Stewart’s wilt disease of maize is caused by Pantoea stewartii subsp. stewartii. This bacterium is seed-borne pathogens, when attacked maize caused yield lost 40-100%. The objective of this research was to detemine the incidence level of stewart’s wilt disease, growth of some varieties of maize and their response to stewart’s wilt pathogens Pantoea stewartii subsp. stewartii. The research was conducted in the Laboratory of Bacteriology and Greenhouse Cikabayan IPB from November 2011 to March 2012. In experiment I, nineteen samples of maize were used for symptom test in the maize seedling stage, using Randomized Block Design with three replications. Experiment II used a Randomized Block Design with 2 factors: maize varieties (8 hybrids varieties, 3 open pollinated varieties, and 7 sweet corn varieties) and bacteria isolates (BGR 2, BGR 4, BGR 28, BGR7 and PSM 27), with three replications. The results showed in experiment I, the incidence of stewart’s wilt disease ranged 2.00 – 15.33%, germination and vigor index of maize seed were 68.00 – 95.33% and 55.33 – 90.67% respectively. While in experiment II, hybrid and open pollinated of maize varieties were resistant to moderately susceptible while all sweet corn varieties were susceptible to infection of Pantoea stewartii subsp. stewartii.