Oleg Vitaljevich Kukushkin

Scopus Publications

Johann Gottlieb Georgi or Peter Simon Pallas: review regarding the authorship and description of Lacerta taurica (Squamata, Lacertidae)
OLEG KUKUSHKIN, FRANK TILLACK, IGOR DORONIN, NIKITA KLUGE, DANIEL JABLONSKI
Zootaxa, 2024
From the beginning of the 19th century to the present, the authorship of the description of the Balkan wall lizard, Podarcis tauricus (Pallas, 1814) was considered undoubted. Recently, Schmidtler (2022) has been concluded that the description of this species should be attributed to J.G. Georgi. Therefore we have chosen to discuss this issue. The description of Lacerta taurica by Georgi in 1801 was based on earlier works by C.L. Hablitz (1785, 1789). However, his description does not align with our current understanding of the morphological and ecological characteristics of the taxon currently recognized as P. tauricus. Some details contained in this description suggest that it was based not on the species recently known as Podarcis tauricus, but on another lizard inhabiting the same place and recently known as Darevskia lindholmi (Szczerbak, 1962). If recognize authorship of the name Lacerta taurica as belonging to Georgi (1801), this would require transferring that name to the species currently known as D. lindholmi and the need to introduce a new name for the species currently known as P. taurica, that will cause numerous confusions. To provide the stability of the names, we suggest to continue to regard Pallas (1814) as the author of the name Lacerta taurica and will apply to the Commissuion of Zoological Nomenlature for conservation of this status. Regrettably, a specimen collected by Pallas himself in Crimea has not been located until now. However, the search for preserved historic specimens continues.
The Silk roads: phylogeography of Central Asian dice snakes (Serpentes: Natricidae) shaped by rivers in deserts and mountain valleys
Daniel Jablonski, Konrad Mebert, Rafaqat Masroor, Evgeniy Simonov, Oleg Kukushkin, et al.
Current Zoology, 2024
Influenced by rapid changes in climate and landscape features since the Miocene, widely distributed species provide suitable models to study the environmental impact on their evolution and current genetic diversity. The dice snake Natrix tessellata, widely distributed in the Western Palearctic is one such species. We aimed to resolve a detailed phylogeography of N. tessellata with a focus on the Central Asian clade with 4 and the Anatolia clade with 3 mitochondrial lineages, trace their origin, and correlate the environmental changes that affected their distribution through time. The expected time of divergence of both clades began at 3.7 Mya in the Pliocene, reaching lineage differentiation approximately 1 million years later. The genetic diversity in both clades is rich, suggesting different ancestral areas, glacial refugia, demographic changes, and colonization routes. The Caspian lineage is the most widespread lineage in Central Asia, distributed around the Caspian Sea and reaching the foothills of the Hindu Kush Mountains in Afghanistan, and Eastern European lowlands in the west. Its distribution is limited by deserts, mountains, and cold steppe environments. Similarly, Kazakhstan and Uzbekistan lineages followed the Amu Darya and the Syr Darya water systems in Central Asia, with ranges delimited by the large Kyzylkum and Karakum deserts. On the western side, there are several lineages within the Anatolia clade that converged in the central part of the peninsula with 2 being endemic to Western Asia. The distribution of both main clades was affected by expansion from their Pleistocene glacial refugia around the Caspian Sea and in the valleys of Central Asia as well as by environmental changes, mostly through aridification.
Comparative characteristics of the postcranial skeleton of Rock lizards from the Darevskia (caucasica) and Darevskia (saxicola) complexes
A.V. Lishchuk, I.V. Doronin, O.V. Kukushkin
Proceedings of the Zoological Institute of the Russian Academy of Sciences, 2024
Anatomical-morphological features of the postcranial skeleton of nine taxa of the rock lizards of the genus Darevskia Arribas, 1999 belonging to the Darevskia (caucasica) and Darevskia (saxicola) complexes were studied for the first time on representative samples. Specimens with the rib formula 3+1 were found in both complexes (previously, only two variants were known for the genus Darevskia 3+2 and 3+3). Differences between supraspecific groups and their members were described with the use of type specimens or topotypes of each taxon. The studied complexes are differentiated by the range of variability of presacral vertebrae (in the D. (caucasica) their minimum number is 26, in the D. (saxicola) – 27), the number of the posterior presacral vertebrae with ribs (the average value of this trait in the D. (caucasica) is less than 6, in D. (saxicola) – more than 6). B-type of the tail vertebrae in 5 times more common in the D. (saxicola) than in the D. (caucasica). In turn, the latter group is characterized by a larger share of individuals with ossified ribs at the third cervical vertebra and a greater variability of the sternal-xiphisternal costal (rib) formula. Skeletal morphology of D. alpina indicates its greater similarity with representatives of the D. (caucasica) complex. A certain originality of D. c. vedenica was recorded. The study of D. saxicola specimens from the zone of sympatry with D. c. caucasica (Chegem Gorge) did not reveal their similarity with the latter, except for the presence of two variants of the sternal-xiphisternal formula. Cryptic forms within D. lindholmi differ at least in two traits: the minimum number of posterior presacral vertebrae with short ribs and the presence of individuals with caudal vertebrae of B-type.
Evolutionary divergence of the smooth snake (Serpentes, Colubridae): The role of the Balkans and Anatolia
Manos Stratakis, Iraklis Koutmanis, Çetin Ilgaz, Daniel Jablonski, Oleg V. Kukushkin, et al.
Zoologica Scripta, 2022
AN EXPERIENCE OF CAPTIVE INCUBATION OF THE CLUTCH OF THE EUROPEAN GLASS LIZARD (REPTILIA: SAURIA: ANGUIDAE), WITH NOTES ON ITS REPRODUCTIVE BIOLOGY
Oleg V. Kukushkin
Russian Journal of Herpetology, 2022
A case of a successful incubation of a clutch of the European glass lizard (Pseudopus apodus) under controlled laboratory conditions during July 22 – September 19, 2019 is reported here. Fully developed neonates hatched from all eggs (n = 7) on the 60th day of incubation. A detailed description of the temperature and humidity regimes of incubation as well as the size and weight characteristics of eggs and offsprings are provided. Moreover, protecting and caring female behavior in regards to the clutch within the first month of incubation was documented comprehensively for the first time. Some aspects of P. apodus reproductive biology such as parental care, oviposition terms, frequency of reproduction and female fecundity are discussed taking into account the data accumulated within the last decennary for the Crimean populations of the species. Likely causes for the non-annual breeding of females were analyzed
Juniperus excelsa s. str. in crimea - differentiation and history inferred from genetic and morphological markers
Małgorzata Mazur, Katarzyna A. Jadwiszczak, Agnieszka Bona, Yuliya Krasylenko, Oleg Kukushkin, et al.
Folia Forestalia Polonica Series A, 2021
Juniperus excelsa s. str. (Greek juniper) in Crimea is a relic species on the limits of its range, and represents the Mediterranean flora in the Sub-Mediterranean part of the peninsula. Its origin and history in this area remains unresolved. We aimed to analyze phylogeny and potential demographic expansion of the juniper in the Crimea as well as to study its morphological differentiation. We analyzed plant material from 59 trees inhabiting eight populations. Genetic variation assessments were based on the four non-coding chloroplast DNA (cpDNA) fragments and the nuclear internal transcribed spacer region ITS1-5,8S-ITS2 (ITS). To examine the morphological differentiation, eight measured/counted traits of cones, seeds, and shoots were chosen and eight ratios were calculated. Morphological parameters were compared using ANOVA, Student’s t test, discrimination analysis and Kruskal-Wallis and U Mann-Whitney tests. Two cpDNA fragments were polymorphic and, in total, 10 cpDNA haplotypes were found. Haplotype diversity (Hd) ranged from 0.0 to 0.9. Based on both cpDNA and ITS sequences variation, phylogenetic analyses revealed a close relationship of the Crimean junipers to the individuals from other parts of the species range. In general, our molecular results confirmed the low level of genetic differentiation of J. excelsa individuals inhabiting different parts of the species range, likely resulting from a common ancestry. Only slight morphological differences were found between populations with different geographic location or habitat. The analyzes showed the distinctness of the populations from the southern part of the coast. Some unique morphological and molecular features of southern coastal populations imply that they are remnants of Late Pleistocene abundant forests. We suggest that the recent fragmentation of the Juniperus populations in the Crimean Peninsula could have arisen during the Atlantic period of the Holocene.
The mitochondrial phylogeography of the Crimean endemic lizard Darevskia lindholmi (Sauria, Lacertidae): Hidden diversity in an isolated mountain system
Oleg Kukushkin, Oleg Ermakov, Iulian Gherghel, Svetlana Lukonina, Anton Svinin, et al.
Vertebrate Zoology, 2021
The Lindholm rock lizard, Darevskia lindholmi, is the only member of the genus Darevskia whose range is restricted solely to Europe, representing a local endemism found only in the Crimean Mountains. In our study, we investigated the cytochrome b gene (mtDNA) of 101 D. lindholmi sequences from 65 Crimean localities, representing its entire range. We found that D. lindholmi is highly genetically structured, and its range is divided into populations belonging to three mitochondrial lineages. The Lindholm rock lizard populations inhabiting the middle part of the Crimean Mountains (further referred to as the Central lineage) are sharply differentiated from the other two lineages (the Common and the Southwestern lineages), which are present in most of the species range. The genetic distance between the Central lineage and the other two taken together is 4.6%, according to our results, suggesting that the divergence occurred during the Early Pleistocene. The narrowly distributed Southwestern lineage and the widespread Common lineage, on the other hand, are differentiated by 1%. Field observations on the representatives of the main evolutionary groups show that their ecology is also different: the Central lineage is a mesophilic and cold-resistant form, while the other two closely related lineages are more xerophilic and thermophilic. Results of the potential ranges modeling and ecological niche analysis confirm that the genetic lineages occupy different niches of the Crimea. Furthermore, the area of inhabitation of the Central lineage splits the western and eastern parts of the Common lineage range, while the Southwestern lineage is restricted along the coast of the southwestern coast of the peninsula. The long-term co-existence of deeply divergent sister mitochondrial lineages in a relatively small (circa 7,000 km2) isolated mountain system serves as a mesocosm for understanding the speciation process. Our data suggest that the Central lineage warrants further taxonomic investigation.
Morphological and genetic differentiation in the anguid lizard Pseudopus apodus supports the existence of an endemic subspecies in the Levant
Daniel Jablonski, Marco Antônio Ribeiro-Júnior, Shai Meiri, Erez Maza, Oleg V. Kukushkin, et al.
Vertebrate Zoology, 2021
The Levant represents one of the most important reptile diversity hotspots and centers of endemism in the Western Palearctic. The region harbored numerous taxa in glacial refugia during the Pleistocene climatic oscillations. Due to the hostile arid conditions in the warmer periods they were not always able to spread or come into contact with populations from more distant regions. One large and conspicuous member of the Levantine herpetofauna is the legless anguid lizardPseudopus apodus. This species is distributed from the Balkans to Central Asia with a portion of its range running along the eastern Mediterranean coast. Mitochondrial and nuclear DNA sequences, microsatellite genotypes, and morphology show that populations in this region differ from the two named subspecies and presumably had a long independent evolutionary history during the Quaternary. Here we describe the Levantine population as a new subspecies and present biogeographic scenarios for its origin and diversification. The new subspecies is genetically highly diverse, and it forms a sister lineage toPseudopusfrom the remaining parts of the range according to mtDNA. It is the largest-bodied of the three subspecies, but occupies the smallest range.
Landscape genomics of a widely distributed snake, dolichophis caspius (Gmelin, 1789) across eastern europe and western asia
Sarita Mahtani-Williams, William Fulton, Amelie Desvars-Larrive, Sara Lado, Jean Pierre Elbers, et al.
Genes, 2020
Across the distribution of the Caspian whipsnake (Dolichophis caspius), populations have become increasingly disconnected due to habitat alteration. To understand population dynamics and this widespread but locally endangered snake’s adaptive potential, we investigated population structure, admixture, and effective migration patterns. We took a landscape-genomic approach to identify selected genotypes associated with environmental variables relevant to D. caspius. With double-digest restriction-site associated DNA (ddRAD) sequencing of 53 samples resulting in 17,518 single nucleotide polymorphisms (SNPs), we identified 8 clusters within D. caspius reflecting complex evolutionary patterns of the species. Estimated Effective Migration Surfaces (EEMS) revealed higher-than-average gene flow in most of the Balkan Peninsula and lower-than-average gene flow along the middle section of the Danube River. Landscape genomic analysis identified 751 selected genotypes correlated with 7 climatic variables. Isothermality correlated with the highest number of selected genotypes (478) located in 41 genes, followed by annual range (127) and annual mean temperature (87). We conclude that environmental variables, especially the day-to-night temperature oscillation in comparison to the summer-to-winter oscillation, may have an important role in the distribution and adaptation of D. caspius.
Cytochrome b mitochondrial gene analysis-based phylogeography of a Sand lizard in the Crimea: Ancient refugium at the peninsula, late expansion from the North, and first evidence of Lacerta agilis tauridica and L. a. exigua (Lacertidae: Sauria) hybridization
O.V. Kukushkin, O.A. Ermakov, A.Yu. Ivanov, I.V. Doronin, E.Yu. Sviridenko, et al.
Proceedings of the Zoological Institute of the Russian Academy of Sciences, 2020
The contact zones of the distribution ranges of closely related reptile taxa are the source of valuable data on the microevolutionary processes in populations, the history of regional faunas origin, and the environmental preferences of the studied forms. Our study is focused on the genetic structure of the populations of sand lizard, Lacerta agilis Linnaeus, 1758, at the Crimean peninsula. This lacertid species inhabits the mountain (afforested) and plain (steppe) parts of the Crimea, being abundant or common in many areas. Lacerta agilis is represented in the Crimea by two subspecies: the widely distributed Eastern (L. a. exigua Eichwald, 1831) inhabiting a large part of Northern Eurasia, and the endemic (L. a. tauridica Suchow, 1927) residing in the Crimean mountains. Mitochondrial haplogroup affiliation corresponding to one of the subspecies (L. a. tauridica, L. a. exigua or L. a. chersonensis) was established for 225 L. agilis individuals from 81 localities in the Crimea and adjacent mainland territories. The nucleotide sequences of the complete cytochrome b gene of mitochondrial DNA (1143 bp) were studied in 75 L. agilis individuals from 68 localities. The genetic distance between both subspecies inhabiting the Crimea revealed by used molecular marker comprised 2.8%, which indicates their early divergence approximately at the transition of Early to Middle Pleistocene (ca. 1 Mya). L. a. tauridica is characterized by a comparatively deep genetic structure. Haplotypes occupying isolated positions on the phylogenetic tree of this subspecies were found in the south-western part of the Crimean Mountains, what might be explained by the localization of L. agilis microrefugia in areas least affected by the Late Pleistocene cooling. Genetic structure of L. a. exigua is more homogeneous. Another important result of our study was an identification of zones of haplogroups «exigua» and «tauridica» coexistence localized along the northern and eastern margins of the Crimean Mountains. The portion of the «exigua» haplogroup in local populations decreases southward and westward. The observed pattern of the spatial distribution of haplogroups seems to be a result of the hybridization zone formation between the sand lizard subspecies during the Holocene expansion of L. a. exigua. Ecological niches modeling for L. agilis subspecies and analysis of morphological variability of the lizards support the hypothesis of L. a. exigua and L. a. tauridica hybridization in the area of contact of their ranges in the eastern part of the Crimean Mountains.
The biogeography of Elaphe sauromates (Pallas, 1814), with a description of a new rat snake species
Daniel Jablonski, Oleg V. Kukushkin, Aziz Avcı, Sabina Bunyatova, Yusuf Kumlutaş, et al.
Peerj, 2019
Complete mitochondrial genome of the Blotched snake, Elaphe sauromates (Pallas, 1814)
Daniel Jablonski, Katarina Soltys, Oleg V. Kukushkin, Evgeniy Simonov
Mitochondrial DNA Part B Resources, 2019
Amphibians and reptiles in the subterranean cavities of the crimean mountains
Ilya S. Turbanov, Oleg Vitaljevich Kukushkin, Robert S. Vargovitsh
Russian Journal of Herpetology, 2019
Herpetofauna of Sevastopol city (southwestern Crimea): species composition, zoogeographic analysis, landscape-zonal distribution, current status and protection
Oleg Kukushkin, Alexander Trofimov, Ilya Turbanov, Victor Slodkevich
Ecosystem Transformation, 2019
Captive breeding of two colubrid species — zamenis situla and elaphe sauromates (Serpentes: Colubridae) and their reproductive biology in the crimea
Ekaterina Aleksandrovna Polyakova, Ilya Sergeevich Korshunov, Svetlana Petrovna Popovskaya, Oleg Vitaljevich Kukushkin
Russian Journal of Herpetology, 2019
Cryptic diversity in the smooth snake (Coronella austriaca)
Daniel Jablonski, Zoltán T. Nagy, Aziz Avcı, Kurtuluş Olgun, Oleg V. Kukushkin, et al.
Amphibia Reptilia, 2019
Multilocus phylogeny and coalescent species delimitation in Kotschy's gecko, Mediodactylus kotschyi: Hidden diversity and cryptic species
Panayiota Kotsakiozi, Daniel Jablonski, Çetin Ilgaz, Yusuf Kumlutaş, Aziz Avcı, et al.
Molecular Phylogenetics and Evolution, 2018
Resolving complex phylogeographic patterns in the Balkan Peninsula using closely related wall-lizard species as a model system
Nikolaos Psonis, Aglaia Antoniou, Emmanouela Karameta, Adam D. Leaché, Panayiota Kotsakiozi, et al.
Molecular Phylogenetics and Evolution, 2018
Sexual dimorphism in Pseudopus apodus (Reptilia: Sauria: Anguidae) from the Steppe Crimea
Ecologica Montenegrina, 2018
Pleistocene extinctions and recent expansions in an anguid lizard of the genus Pseudopus
David Jandzik, Daniel Jablonski, Oleksandr Zinenko, Oleg V. Kukushkin, Jiří Moravec, et al.
Zoologica Scripta, 2018
Juniper dwarf mistletoe (Arceuthobium oxycedri) in the Crimean Peninsula: Novel insights into its morphology, hosts, and distribution
Yuliya A. Krasylenko, Kateřina Janošíková, Oleg V. Kukushkin
Botany, 2017
Hidden diversity in the Podarcis tauricus (Sauria, Lacertidae) species subgroup in the light of multilocus phylogeny and species delimitation
Nikolaos Psonis, Aglaia Antoniou, Oleg Kukushkin, Daniel Jablonski, Boyan Petrov, et al.
Molecular Phylogenetics and Evolution, 2017
A record of the Balkan Stripe-necked Terrapin, Mauremys rivulata (Testudines: Geoemydidae) from the Azov Sea Coast in the Crimea
Amphibian and Reptile Conservation, 2016
Mitochondrial phylogeny shows multiple independent ecological transitions and northern dispersion despite of Pleistocene glaciations in meadow and steppe vipers (Vipera ursinii and Vipera renardi)
Oleksandr Zinenko, Nikolaus Stümpel, Lyudmila Mazanaeva, Andrey Bakiev, Konstantin Shiryaev, et al.
Molecular Phylogenetics and Evolution, 2015
A new species of mites of the genus geckobia (Prostigmata, Pterygosomatidae), parasitic on mediodactylus kotschyi (Reptilia, Gekkota) from Crimea
M. Bertrand, O. Kukushkin, S. Pogrebnyak
Vestnik Zoologii, 2013

Oleg Vitaljevich Kukushkin

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Scopus Publications