J Ramesh Kumar

Scopus Publications

Fabrication and optimization of naringin-loaded MOF-5 encapsulated by liponiosomes as smart drug delivery, cytotoxicity, and apoptotic on breast cancer cells
Lina M. Alneghery, Mohammed Al-Zharani, Fahd A. Nasr, Zienab E. Eldin, Tayel A. Al Hujran, Hesham M. Tawfeek, Mohamed H. Fayed, Shehab Elbeltagi
Drug Development and Industrial Pharmacy, 2025
INTRODUCTION Cancers are regarded as hazardous due to their high worldwide death rate, with breast cancer (BC), which affects practically all cancer patients globally, playing a significant role in this statistic. The therapeutic approach for BC has not advanced using standard techniques, such as specialized naringin (NG) chemotherapy. Instead, a novel strategy has been utilized to enhance smart drug delivery (SDD) to tumors.Significance: Herein, we established NG-loaded zinc metal-organic framework-5 (NG-MOF-5) coated with liponiosomes (LNs) to manufacture NG-MOF-5@LNs nanoparticles (NPs) for antibacterial and cancer treatment. METHODS MOF-5, NG, and NG-MOF-5@LNs were evaluated with XRD, TGA, FTIR, SEM, TEM, PDI, ZP, encapsulation efficiency (EE), loading efficiency (LE), and drug-release (DR) kinetics. We examined the antibacterial activity involving minimum inhibitory concentration (MIC) and zone of inhibition by NG, MOF-5, and NG-MOF-5@LNs. The cell viability, necrosis, and total apoptosis (late and early) were evaluated for anti-cancer activity against MCF-7 BC cells. RESULTS TEM results demonstrated that NG-MOF-5@LNs formed monodispersed spherical-like particles with a size of 122.5nm, PDI of 0.139, and ZP of +21mV. The anti-microbial activity results indicated that NG-MOF-5@LNs exhibited potent antibacterial effects, as evidenced by inhibition zones and MIC values. The Higuchi model indicates an excellent fit (R2=0.9988). The MTT assay revealed anti-tumor activity against MCF-7 BC cells, with IC50 of 21.6µg/mL for NG-MOF-5@LNs and demonstrating a total apoptosis effect of 68.2% on MCF-7 cells. CONCLUSIONS NG-MOF-5@LNs is anticipated to show as an effective antimicrobial and novel long-term-release antitumor agent and might be more suitable for MCF-7 cell therapy.
A novel zingerone-loaded zinc MOF coated by niosome nanocomposites to enhance antimicrobial properties and apoptosis in breast cancer cells
Hanan M. Alharbi, Abdel-Nasser M.A. Alaghaz, Tayel A. Al Hujran, Zienab E. Eldin, Shehab Elbeltagi
Materials Today Communications, 2024
Bioactive compounds and profiling of anticancer and antioxidant activities of Euphorbia paralias L. family Euphorbiaceae
Ahmed M.M. Youssef, Thabet H. Althneibat, Doaa A.M. Maaty, Tayel A. Al Hujran, Yousef M. Al-Saraireh
Journal of Pharmacy and Pharmacognosy Research, 2024
Context: Medicinal plants are considered the bases for chemical compounds that could be used therapeutically. Euphorbia paralias belongs to the family Euphorbiaceae. Some species of this family have demonstrated anticancer and antioxidant activities. However, the chemical compounds responsible for the anticancer and antioxidant activities of E. paralias have not been extensively studied. Aims: To identify chemical compounds of E. paralias that possess anticancer and antioxidant properties. Methods: Liquid chromatography/mass spectrometry (LC/MS) was employed to characterise the chemical compounds E. paralias methanolic extract. Using the MTT assay, anticancer activity was investigated. DPPH, ABTS, and FRAP tests were applied to assess the antioxidant action. Results: Several polyphenolic compounds, including phenolic acids, flavonoids, flavanols, fatty acids, terpenoids, and sterols, were identified in the extract. These compounds possess various biological activities. The cancer cell lines most affected by the E. paralias extract were PANC-1 with an IC50 of 60.1 ± 10.1 µg /mL, DLD-1 with an IC50 of 64.3 ± 17.5 µg/mL, and A549 with IC50 of 71.9 ± 9.8 µg/mL. The values of IC50 for the MCF-7 and U87 cancer cell lines were 74.6 ± 15.6 µg/mL and 121.3 ± 17.2 µg/mL, respectively. The cell line of normal HDF had an IC50 of 128.9 ± 11.7 µg/mL. ABTS and DPPH showed that the methanol extract scavenged the free radicals by 89.57% and 92.65%, respectively, compared to ascorbic acid and Trolox positive controls. The FRAP test showed that 1 mg of methanol extract was equivalent to 387.41 μM of Trolox. Conclusions: E. paralias contained various phytochemicals that may have anticancer and antioxidant activities. E. paralias may possess anticancer activity against pancreatic, colon and lung cancers, and it may also have antioxidant activity. Future research is recommended to investigate the isolated phytochemicals from E. paralias.
Insight into the Inclusion Complexation of Fluconazole with Sulfonatocalix[4]naphthalene in Aqueous Solution, Solid-State, and Its Antimycotic Activity
Tayel A. Al Hujran, Mousa K. Magharbeh, Almeqdad Y. Habashneh, Rasha S. Al-Dmour, Ashraf Aboelela, Hesham M. Tawfeek
Molecules, 2022
The study aims to assess the interaction between fluconazole and sulfonatocalix[4]naphthalene towards enhancing its dissolution performance and antimycotic activity. A solubility study was carried out at different pH conditions, and the results revealed the formation of a 1:1 molar ratio fluconazole-sulfonatocalix[4]naphthalene inclusion complex with an AL type phase solubility diagrams. The solid powder systems of fluconazole-sulfonatocalix[4]naphthalene were prepared using kneaded and co-evaporation techniques and physical mixtures. DCS, PXRD, TGA-DTG, FT-IR, and in vitro dissolution performance characterize the prepared systems. According to physicochemical characterization, the co-evaporation approach produces an amorphous inclusion complex of the drug inside the cavity of sulfonatocalix[4]naphthalene. The co-evaporate product significantly increased the drug dissolution rate up to 93 ± 1.77% within 10 min, unlike other prepared solid powders. The antimycotic activity showed an increase substantially (p ≤ 0.05, t-test) antimycotic activity of fluconazole co-evaporate mixture with sulfonatocalix[4]naphthalene compared with fluconazole alone against clinical strains of Candida albicans and Candida glabrata. In conclusion, sulfonatocalix[4]naphthalene could be considered an efficient complexing agent for fluconazole to enhance its aqueous solubility, dissolution performance, and antimycotic activity.
Analysis of Metals in Cataract Fluid of Jordanian Patients by Using the Inductively Coupled Plasma Optical Emission Spectrometry
Fawaz Al-Saraireh, Abdul-Wahab R. Hamad, Mousa Khames Mezel Magarbeh, Tayel A. AL-Hujran, Mutasem Elfalah
Biomedical and Pharmacology Journal, 2022
Background: The study was completed to determine the level of the following metals (Sodium (Na), Potassium (K), Phosphorus (P), Calcium (Ca), Manganese (Mn), Iron (Fe), Cobalt (Co), Copper (Cu), Zinc (Zn), Selenium (Se), and Cadmium (Cd)) in different cataractous human lenses. Materials and Methods: Five Samples had collected from the normal individuals healthy people and 25 suffered from cataracts at the Italian Hospital, Al-Karak, Jordan. This study had conducted between November 2020 and October 2021 on 30 individual subjects. Samples fluid from the Cataract sufferers had been gathered, the data was analyzed using Microsoft excels and SPSS software program new version. The trace elements concentration withinside the aspirated fluid were measured with the inductively coupled plasma optical emission spectrometry – Mass (ICP-MS), best controlled with certified standards. Results: The mean values of copper were (12.9±12.4) and (7.4±9.7) for patients and control groups respectively. The average mean values of Cd were found to be (5.9 ±2.4) and (4.6 ± 2.4). The cadium, copper, manganese, magnesium and potassium levels were significantly higher in the catarctous groups. The others elements were lower in the catarctous group than control. Conclusion: Results showed significant change in some metallic elements concentration with cataract. These consequences can be beneficial for understanding and identifying the reasons that can play a function withinside the initiation and improvement of cataract. These factors deficiency may also have some suggestions to diets and diseases via its appropriate concentrations and its consequences on various organs parameters. It appears that the estimation of serum or plasma concentration of these metals may also assist in research and remedy of other diseases in both genders.
Spectrophotometric analysis of empagliflozin tablets as SGLT2 inhibitors in pharmaceutical samples
Wael Abu Dayyih, Mohammad Hailat, Tayel A. Al Hujran, Mousa Magharbeh, Zainab Zakaraya, Lina Al Tamimi, Aseel M. Aburumman, Hamza Abumansour, Riad Awad
Journal of Applied Pharmaceutical Science, 2022
Azo dyes account for 70% of dye chemistry, and their importance may grow in the future. Empagliflozin is a sodium-glucose co-transporter-2 (SGLT2) inhibitor. SGLT2 transporters are primarily responsible for glucose reabsorption in the kidney. In 2014, empagliflozin was approved for medical use in the United States and the European Union. With over 4 million prescriptions in 2019, it was the 146th most commonly prescribed medication in the United States in 2019. The spectrophotometric determination of empagliflozin is described using coupling agents such as 3-chloro-4-nitroaniline or sulfanilamide. These methods are straightforward and are based on the reaction of empagliflozin with diazotized products of 3-chloro-4-nitroaniline or sulfanilamide to produce colored azo dyes with absorption maxima at 470 and 480 nm. Empagliflozin was linear from 1.2 to 26.6 µgml −1 or 0.8 to 20.4 µgml −1 when combined with diazotized 3-chloro-4-nitroaniline or sulfanilamide, respectively. Empagliflozin’s molar absorptivity and Sandell’s sensitivity to 3-chloro-4-nitroaniline or sulfanilamide azo dyes were 3.179 × 10 4 l mol −1 cm −1 or 4.367 × 10 4 l mol −1 cm −1 and 1.149 × 10 −2 µgcm −2 or 8.368 × 10 −3 µgcm −2 , respectively. The formed colored azo dyes are stable for more than 12 hours. The optimal reaction conditions and other analytical parameters are assessed. Foreign organic compound interference has been studied. The method has been successfully used to determine empagliflozin in pharmaceutical samples.
GC-mass determination for the biodegradative products of 2,6-dimethylpyridine using dead-sea bacterial isolate
Research Journal of Chemistry and Environment, 2021
Studying the complex formation of sulfonatocalix[4]naphthalene and meloxicam towards enhancing its solubility and dissolution performance
Tayel A. Al Hujran, Mousa K. Magharbeh, Samer Al-Gharabli, Rula R. Haddadin, Manal N. Al Soub, Hesham M. Tawfeek
Pharmaceutics, 2021
The interaction between meloxicam and sulfonatocalix[4]naphthalene was investigated to improve the meloxicam solubility and its dissolution performance. Solubility behavior was investigated in distilled water (DW) and at different pH conditions. Besides, solid systems were prepared in a 1:1 molar ratio using coevaporate, kneading, and simple physical mixture techniques. Further, they were characterized by PXRD, FT-IR, DCS, and TGA. In vitro dissolution rate for coevaporate, kneaded, and physical mixture powders were also investigated. Solubility study revealed that meloxicam solubility significantly increased about 23.99 folds at phosphate buffer of pH 7.4 in the presence of sulfonatocalix[4]naphthalene. The solubility phase diagram was classified as AL type, indicating the formation of 1:1 stoichiometric inclusion complex. PXRD, FT-IR, DCS, and TGA pointed out the formation of an inclusion complex between meloxicam and sulfonatocalix[4]naphthalene solid powders prepared using coevaporate technique. In addition, in vitro meloxicam dissolution studies revealed an improvement of the drug dissolution rate. Furthermore, a significantly higher drug release (p ≤ 0.05) and a complete dissolution was achieved during the first 10 min compared with the other solid powders and commercial meloxicam product. The coevaporate product has the highest increasing dissolution fold and RDR10 in the investigated media, with average values ranging from 5.4–65.28 folds and 7.3–90.7, respectively. In conclusion, sulfonatocalix[4]naphthalene is a promising host carrier for enhancing the solubility and dissolution performance of meloxicam with an anticipated enhanced bioavailability and fast action for acute and chronic pain disorders.
Bioavailability and bioequivalence of two oral single dose of ibuprofen 400 mg to healthy volunteers
Saied M. I. Al-Dalaen, Abdul-Wahab R. Hamad, Tayel A. AL-Hujran, Hayat A. Al-Btoush, Lidia Al-Halaseh, Mousa K. Magharbeh, Nariman A. Al-Jawabri, Islam A. Al-Kasasbeh, Fadhil M. Abid
Biomedical and Pharmacology Journal, 2021
Objective: The objective of the two pharmacokinetic studies reported here was to compare the relative bio availability and bio equivalence of an ibuprofen 400 mg tablet from National Company (SDI) as a test with a reference formulation. Study Design: Evaluation of two open, randomized, cross-over studies, one single dose in healthy male volunteers. Methods: 20 healthy volunteers were randomized in a cross-over design to single dose of Profedin 400 mg produced from National Company, ibuprofen formulation, as a test and a reference formulation produced from Pharmacia & Upjohn, Ibuprofen 400 mg. Ibuprofen and standard of ibuprofen were analyzed by utilizing HPLC, the sample extracted from 0.5ml of plasma with an organic solution of isooctane and 2-propanol. The mobile phase consisted of 44% acetonitrile and 0.1% phosphoric acid. The flow rate was 1 ml/min. The analytical column was a C-18, 5um packing size. Detection of Ibuprofen and the internal standard occurred by UV absorbance at wavelength of 220 nm. Results: A single-dose study demonstrated that the bio availability of ibuprofen for both formulations was not significantly different. In addition, mean plasma levels of ibuprofen predictive of clinical efficacy were achieved within 1.5- 2.0 hours and the elimination of ibuprofen tablets is virtually complete in 12 hours after the single dose. The serum half-life is 1.8 to 2.0 hours. The Cmax, Tmax, Kelemin.0.5 were calculated for the test and reference. They were not significantly different. Conclusions: Blood levels predicted that the present slow-release formulation of ibuprofen should offer reliable day and night control of pain and fever and is associated with a favorable safety profile.
Phytochemical and anti-cancer properties of Euphorbia hierosolymitana Boiss. crude extracts
Journal of Pharmacy and Pharmacognosy Research, 2021
Assessment of paronychia argentea extraction on kidney stone by using calcium oxalate method
Mousa K. Magharbeh, Tayel A. Al-Hujran, Saied M. I. Al-Dalaen, Abdul-Wahab R. Hamad
Biomedical and Pharmacology Journal, 2020
Phytochemical screening and in vitro antioxidant and antiurolithic activities of Coffea arabica
Research Journal of Chemistry and Environment, 2020
Surface relief grating growth in thin films of mexylaminotriazine-functionalized glass-forming azobenzene derivatives
Othmane R. Bennani, Tayel A. Al-Hujran, Jean-Michel Nunzi, Ribal Georges Sabat, Olivier Lebel
New Journal of Chemistry, 2015
Synthesis and complexation of a mixed-pyridine-naphthalene homooxacalixarene
Tayel A. AlHujran, Shofiur Rahman, Louise Nicole Dawe, Paris Elias Georghiou
Supramolecular Chemistry, 2014
Synthesis of functionalized acenaphthenes and a new class of homooxacalixarenes
Tayel A. AlHujran, Louise N. Dawe, Paris E. Georghiou
Organic Letters, 2012
Corannulene and its penta-tert-butyl derivative co-crystallize 1: 1 with pristine C60-fullerene
Louise N. Dawe, Tayel A. AlHujran, Huu-Anh Tran, Jason I. Mercer, Edward A. Jackson, Lawrence T. Scott, Paris E. Georghiou
Chemical Communications, 2012
Synthesis and Clathrates of Oligomeric 2-O-Naphthoide Macrocycles
Tayel A. AlHujran, Louise N. Dawe, Julie Collins, Paris E. Georghiou
Journal of Organic Chemistry, 2011

J Ramesh Kumar

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