Department of Agriculture and Integrated Cropping Research Center, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran islamic azad university
Evaluating the Efficacy of Exogenous Biostimulants Based on Amino Acids Supplementation on Growth, Physiology, and Oil Composition of Thyme (Thymus vulgaris) under Varying Irrigation Regimes A. Kazempour, Y. Sharghi, S. A. M. M. Sanavy, H. Zahedi, F. Sefidkon Russian Journal of Plant Physiology, 2025 Abstract The objective of this study was to determine the most effective amino acids for enhancing thyme performance and essential oil production under both optimal and water-stressed conditions. A split-plot experiment was conducted, with irrigation level (100% field capacity—optimal, 70%—mild stress, 40%—severe stress) as the main plot factor and treatments (40 mg/L each of aspartic acid, tyrosine, proline, commercial amino acid mixture ‘Aminofert’, and water control) at a volume of 400 L/ha as the subplot factor. Amino acid treatments were applied as foliar sprays every two weeks from 30 days after transplanting until the beginning of flowering (six sprays in total). The amino acid solutions were freshly prepared for each spray and applied using a backpack sprayer at a volume of 400 L/ha. Each solution was supplemented with 0.1% (v/v) Tween-20 as a surfactant. Control plants were sprayed with water containing 0.1% Tween-20. Under optimal irrigation, Aminofert and aspartic acid were superior, increasing growth traits like height, dry weights, and leaf area by 9.7–63.5%. For essential oil, Aminofert enhanced percentage by 28.7% and yield by 69.9%, while aspartic acid improved percentage by 31.6% and yield by 64.0%. These treatments also stimulated physiological enhancements, with Aminofert boosting chlorophyll, carotenoids, and antioxidant enzymes under optimal conditions. Under stress conditions, proline and tyrosine were the most effective amino acids. They enhanced growth by 16.4–79.1% and increased essential oil yield by 52.5–94.9% under mild and severe stress, respectively. Aspartic acid and tyrosine also elevated valuable terpene compounds like carvacrol, E-caryophyllene, and germacrene D. Proline was particularly noteworthy, improving chlorophyll, proline content, and antioxidants under severe stress. The findings suggest strategic amino acid application, especially Aminofert, aspartic acid, proline, and tyrosine, can be a promising approach to enhance crop performance, essential oil yield and quality, and physiological adaptations, particularly under water deficit stress conditions. The differential responses highlight the importance of considering specific amino acid treatments for targeted outcomes.
Effects of Different Irrigation Levels and Phosphate Fertilizer Doses with or without Nano Iron Application on Sugar Beet Growth and Physiology K. Katal, A. Alipour, H. Zahedi, Y. Sharghi, M. Alavifazel Russian Journal of Plant Physiology, 2024 Abstract Despite the importance of phosphate and iron in plant nutrition, their combined effects on sugar beet’s physiological and biochemical responses to drought stress remain unexplored. This study aims to fill this gap and optimize nutrient management for enhanced drought tolerance. A split-plot design with three irrigation regimes (optimal, mild, and severe stress) as main plots and 6 combinations of two iron (F0 and F5 are 0 and 5 kg/ha, respectively) and three phosphorus (P0, P75, and P150 are 0, 75, and 150 kg/ha, respectively) fertilizers as subplots. Medium (P75) and high (P150) phosphate doses, particularly with iron (Fe5), enhanced antioxidant enzymes, catalase and superoxide dismutase, by up to 72%, photosynthetic efficiency (chlorophyll content, Fv/Fm, photosynthetic rate) by up to 107%, and osmolyte accumulation (proline and soluble sugars) by up to 73%. These treatments improved water relations, increasing relative water content by up to 56%, reducing vapor pressure deficit by up to 57%, and making osmotic potential (ψs) up to 57% less negative. However, oxidative stress (malondialdehyde) increased by up to 200%. Despite this, the improved physiological and biochemical functions enhanced white sugar yield by up to 59% and root yield by up to 56%, highlighting the effectiveness of these treatments in increasing sugar beet productivity under drought stress. In conclusion, medium and high phosphate doses, especially when combined with iron, improved drought tolerance and yield in sugar beet under stress by enhancing antioxidant activities, photosynthetic efficiency, osmolyte accumulation, and water relations while reducing oxidative stress and water loss.
Impact of Amino Acid Foliar Applications on Soybean under Optimal and Water-Deficit Conditions: Photosynthesis, Antioxidants, Osmotic Adjustment, and Fatty Acids Z. Behroshan, H. Zahedi, A. Alipour, Y. Sharghi, A. Zand Russian Journal of Plant Physiology, 2024 Abstract Amino acids aid plant stress tolerance, but their precise role in soybean drought physiology remains unclear. This study included amino acid spray treatments (cysteine, valine, leucine, commercial mix) as subplots and irrigation levels (optimal, mild, severe stress) as main plots. Under optimal irrigation, amino acid and lysine performed best for Fv/Fm, while cysteine performed best under severe stress. For chlorophyll content, cysteine, valine and lysine performed best with optimal irrigation, with little difference across water stress conditions. Under optimal irrigation, leucine and valine had the highest SOD levels, while leucine, valine and commercial amino acids performed best under mild stress. Cysteine and valine showed higher CAT under severe stress. Leucine had the lowest MDA without stress, while the commercial mix performed best under mild stress. Valine, amino acids and cysteine had the highest proline without stress and under mild stress, while cysteine and lysine performed best under severe stress. Minimal differences occurred without stress, while valine, amino acids and cysteine performed best under stress. In 2020, amino acids and valine had the highest photosynthesis, while cysteine performed best under severe stress. Valine had the highest mesophyll conductance, while cysteine and valine performed well in 2021. Valine had the highest CO2 and stomatal conductance without stress. All treatments showed superior transpiration. Without stress in 2020, cysteine, valine and amino acids had the highest oil yield, while leucine had it in 2021. Modulation of osmolytes like proline and antioxidants such as SOD and CAT helped valine and cysteine protect soybean plants against water stress.
Comparing Effects of Folic Acid, Epibrassinolide, Chitosan and Glutathione Foliar Treatments on Safflower’s Physiology and Yield during Water Stress N. Abdiazar, H. Zahedi, Y. Sharghi, S. A. M. Modarres-Sanavy, A. Alipour Russian Journal of Plant Physiology, 2024 Abstract While the potential for biostimulants to mitigate water stress holds promise, the underlying physiological mechanisms in safflower (Carthamus tinctorius L.) regarding this matterremain unclear. Elucidating how biostimulants treatments may induce adaptive changes in ROS scavenging, osmoregulation, carbon fixation, gas exchange, and provides insight into optimizing plant water stress tolerance at the cellular level. This split-plot study included biostimulant foliar treatments (folic acid, epibrassinolide, chitosan, glutathione) as subplots, and irrigation regimes (optimal, mild, severe water deficit) as main plots. Under mild water stress, folic acid and epibrassinolide sprays exhibited the highest increase in chlorophyll a (55.3%) and chlorophyll b (51.0%). These treatments also showed the most substantial increases in carotenoids (36.1%), relative water content (18.5%), and soluble proteins (20%). Proline levels were highest with glutathione and chitosan treatments, which increased levels by 81% compared to the control. Transpiration rates were 30% higher on average with folic acid, epibrassinolide, chitosan, and glutathione treatments. Folic acid led to the highest grain yield increase (26%) and GPX levels (22.9%). Severe water stress saw epibrassinolide and glutathione treatments increase relative water content the most (20%). Chitosan led to the highest increases in soluble proteins (23.2%) and transpiration (97%). Proline levels were 59.7% higher on average with folic acid, epibrassinolide, and chitosan treatments. GPX levels increased the most with epibrassinolide, chitosan, and glutathione (62%). Fv/Fm ratios were 170 and 50% higher with folic acid and chitosan or epibrassinolide and glutathione, respectively. Overall, folic acid and epibrassinolide performed best under mild stress, maintaining photosynthesis and yields through increasing chlorophyll, proteins, and water retention. Epibrassinolide, chitosan, and glutathione were most effective under severe stress, conferring drought tolerance by improving antioxidant defenses, protein levels, and water retention.
Effects of seed priming with gibberellin on germination of safflower under salinity stress M. Nejati, A. Alipour, M. Yousefirad, Yones Sharghi, H. Zahedi Iranian Journal of Plant Physiology, 2020 Salinity is one of the most important factors limiting plant growth and production around the world. Gibberellic acid is known as a strong growth stimulator, effective on seed germination and breaking of seed dormancy in various plant species. In order to study the response of Safflower,Sofeh cultivar, to salinity stress, a factorial experiment was conducted based on a randomized complete block designwith three replications in Pardis Agricultural Research and Education Center, Saveh, Iran. The first factor was salinity at three levels including 0, 100, and 200 mMNaCal. The second factor was seed priming with gibberellin at three levels including 0, 20, and 40 mgl-1. Findings suggested that the salinity and seed priming improved biochemical indexes of proline and peroxidase in the seedlings under study, increasing their mean germination time, germination rate, germination percentage, and chlorophyll contents. The maximum proline contents were observed in the seedlings treated with 200 mM salinity and priming with 49 mgl-1gibberelin, 3.48 mg per gram fresh weight. The maximum mean germination time, germination rate, germination percentage, and chlorophyll contents were related to 0 mM salinity and 40 mgl-1gibberellin as 2.86 days, 26.16%, 76.6%, and 8.05%, respectively. In sum, the findings suggested that priming of safflower seeds with gibberellin under saline conditions improves the plants’ resistance against salinity and their germination and growth.
Investigating the effects of various magnetic field exposure intensities and periods in pigweed (Amaranthus retroflexus) seeds Naser Abdiazar, H. Zahedi, Y. Sharghi, S. Sharmili Indian Journal of Agricultural Research, 2019 The present study was conducted to investigate the effects of electromagnetic field on germination characteristics of pigweed (Amaranthus retroflexus). It was conducted in factorial randomized complete design with three replications in Tarbiat modares University, during 2013-14. Pigweed seeds were subjected to three magnetic field levels created by magnets with 4000G, 5000G and 6000G for exposure times of 5, 35 and 65 minutes followed by which the seeds were placed in petri dishes and transferred on germinator. The germination characteristics, germination percentage, time to 50% germination, germination evenness and the rate of germination were recorded .The sprouts were bisected in four-leaf stage and they were evaluated in their morphologic characteristics and the amount of protein and â-amylase enzymatic activity. Results indicated that the mutual effect of the magnetic field exposure duration and intensity was found significant on all the examined traits in pigweed. The highest and lowest germination in pigweed seeds were recorded with 5000G-5minutes and 6000G-65minutes treatments, respectively. The treatments in which the weed seeds were exposed to 5000G intensity for 65minutes, 6000G for 35minutes produced the highest and the lowest amounts of protein, respectively. Based on the experiment results the magnetic field exposure intensity and duration influence the germination characteristics, protein content and â-amylase enzymatic activity rate, this can be a promised method in controlling the weeds in farms.
The survey of yield and components yield in winter safflower cultivar (Carthamus tinctorios L) Advances in Environmental Biology, 2014
The effects of different soil irrigation on yield and its related traits in spring safflower cultivars in Iran Advances in Environmental Biology, 2014
Effects of drought stress treatments on agronomic traits of rapeseed cultivars (Brassica napus L.) Advances in Environmental Biology, 2011
Evaluation of different mycotoxin binders on aflatoxin B1 (Aspergillus parasiticus) produced on rice (Oriza sativa) on egg production and egg quality parameters Advances in Environmental Biology, 2011
Effect of inoculation with biological fertilizers on germination and early growth nitragin cumin and caraway Australian Journal of Basic and Applied Sciences, 2011
The study of planting density on some agronomic traits of spring Canola cultivars Australian Journal of Basic and Applied Sciences, 2011
Evaluating the Efficacy of Exogenous Biostimulants Based on Amino Acids Supplementation on Growth, Physiology, and Oil Composition of Thyme (Thymus vulgaris) under Varying … A Kazempour, Y Sharghi, S Sanavy, H Zahedi, F Sefidkon Russian Journal of Plant Physiology 72 (4), 105 , 2025 2025 Citations: 1
Improving phosphorus use efficiency and quantitative and qualitative yield of sugar beet through combined application of phosphorus and iron nanoparticles under different … K Katal, A Alipour, H Zahedi, Y Sharghi, M Alavifazel Egyptian Journal of Agricultural Research 102 (4), 726-742 , 2024 2024 Citations: 1
Effects of different irrigation levels and phosphate fertilizer doses with or without nano iron application on sugar beet growth and physiology K Katal, A Alipour, H Zahedi, Y Sharghi, M Alavifazel Russian Journal of Plant Physiology 71 (6), 190 , 2024 2024 Citations: 3
Differential effects of foliar and seed priming glycine betaine application on quinoa physiology under varying salinity level A Vali, H Zahedi, A Alipour, Y Sharghi, MR Naeini South African Journal of Botany 175, 253-267 , 2024 2024 Citations: 2
Impact of Amino Acid Foliar Applications on Soybean under Optimal and Water-Deficit Conditions: Photosynthesis, Antioxidants, Osmotic Adjustment, and Fatty Acids Z Behroshan, H Zahedi, A Alipour, Y Sharghi, A Zand Russian Journal of Plant Physiology 71 (3), 88 , 2024 2024 Citations: 2
Investigating some morphological traits and yield of soybean under deficit irrigation and foliar application of amino acids Z Behroshan, H Zahedi, A Alipour, Y Sharghi, A Zand Crop Science Research in Arid Regions 6 (1), 51-67 , 2024 2024
Enhancing safflower seedlings' tolerance to osmotic stress through seed priming with glutathione, epibrassinolide, chitosan, and folic acid N Abdiazar, H Zahedi, Y Sharghi, SAM Modarres Sanavy, A Alipour Egyptian Journal of Agricultural Research 102 (2), 208-216 , 2024 2024 Citations: 2
Comparing effects of folic acid, epibrassinolide, chitosan and glutathione foliar treatments on safflower’s physiology and yield during water stress N Abdiazar, H Zahedi, Y Sharghi, SAM Modarres-Sanavy, A Alipour Russian Journal of Plant Physiology 71 (1), 35 , 2024 2024 Citations: 3
Effect of amino acid foliar application on morphophysiological characteristics and thyme essential oil under different irrigation regimes A Kazempour, Y Sharghi, SAM Modarres Sanavi, H Zahedi Journal of Plant Process and Function 12 (53), 71-90 , 2023 2023 Citations: 4
Effects of seed priming with gibberellin on germination of safflower under salinity stress M Nejati, A Alipour, MY Rad, Y Sharghi, H Zahedi Iranian Journal of Plant Physiology 11 (4) , 2021 2021 Citations: 1
Study of Drought Tolerance of Wheat Inoculated with Mycorrhiza Fungi and its Interaction with Copper Nanoparticles T Hesari, H Zahedi, A Eivazi, Y Sharghi, A Alipoor Journal of Plant Ecophysiology 12 (43), 136-148 , 2020 2020
Effects of seed germination with gibberellin on safflower germination under salinity stress M Nejati, A Alipour, M Yousefirad, Y Sharghi, H Zahedi Iranian Journal of Plant Physiology 5 (1), 3443 , 2020 2020 Citations: 4
Effect of some organic fertilizers on yield and yield components of barley. H Zahedi, Y Sharghi 2019 Citations: 2
Investigating the effects of various magnetic field exposure intensities and periods in pigweed (Amaranthus retroflexus) seeds N Abdiazar, H Zahedi, Y Sharghi Indian Journal of Agricultural Research 53 (2), 233-236 , 2019 2019 Citations: 2
Interaction effect of zeolite and salt stress in reproductive stage of four canola varieties V Esmaeilzadeh, H Zahedi, Y Sharghi, SAM Modarres Sanavy, ... Environmental Stresses in Crop Sciences 11 (2), 393-400 , 2018 2018 Citations: 7
Effect of calcium silicate on salt stress tolerance of four canola varieties M Sanavi, SA Mohammad Iranian Journal of Field Crop Science 49 (1), 179-188 , 2018 2018 Citations: 4
اثر سیلیکات کلسیم بر مقاومت به تنش شوری در چهار رقم کلزا گلستانیان, شرقی, زاهدی, مدرس ثانوی, سید علی محمد, علوی اصل علوم گیاهان زراعی ایران 49 (1), 179-188 , 2018 2018
Determination of Alluvium thickness in LeylanChai dam site using refraction seismic method S Rostami, Y Sharghi 18th Iranian geophysics conference, 482-485 , 2018 2018 Citations: 5
Effect of triazoles foliar application and calcium silicate on canola (Brassica napus L.) dryness tolerance R Monshi, Y Sharghi, H Zahedi, SAM Modarres Sanavy, ... Iranian Journal of Field Crop Science 48 (2), 303-317 , 2017 2017 Citations: 1
Effect of zeolite and calcium silicate on salt stress tolerance of two canola varieties. SS Ghoreishiasl, H Zahedi, Y Sharghi, S Sanavy, MM Ghahderijani 2017 Citations: 5
MOST CITED SCHOLAR PUBLICATIONS
Evaluation of nano biofertilizer efficiency on agronomic traits of spring wheat at different sowing date M Mardalipour, H Zahedi, Y Sharghi Biological forum 6 (2), 349 , 2014 2014.0 Citations: 77
Yield and yield components of six canola ( Brassica napus L.) cultivars affected by planting date and water deficit stress Y Sharghi, AHS Rad, AA Band, G Noormohammadi, H Zahedi African Journal of Biotechnology 10 (46), 9309-9313 , 2011 2011.0 Citations: 75
Effects of late-season drought stress on some physiological traits, yield and yield components of wheat genotypes M Aghanejad, S Mahfoozi, Y Sharghi Biological Forum 7 (1), 1426 , 2015 2015.0 Citations: 32
Effect of foliar application of nano iron and manganese chelated on yield and yield component of canola ( Brassica napus L.) under water-deficit stress at different … L Pourjafar, H Zahedi, Y Sharghi 2016.0 Citations: 23
Respond of saffron (Crocus sativus L.) to animal manure application M Mohammad, ME Amiri, Y Sharghi Journal of Medicinal Plants Research 6 (7), 1323-1326 , 2012 2012.0 Citations: 19
The additional effects of aflatoxin and T-2 toxin combination on commercial broilers: I: Effects on performance parameters and internal organs Y Sharghi, M Manafi Research Journal of Fisheries and Hydrobiology 6 (4), 549-553 , 2011 2011.0 Citations: 18
Comparative assessment of conventional and organic nutrient management on yield and yield components of three corn cultivars. Z Amanolahi-Baharvand, H Zahedi, Y Sharghi, S Seifolahi-Nik 2014.0 Citations: 11
The study of planting density on some agronomic traits of spring canola cultivars. H Bagheri, Y Sharghi, M Yazdani 2011.0 Citations: 11
Estimation of Lugeon number at the abutments of Bakhtyari dam site using seismic tomography Y Sharghi, H Siahkoohi, F Alinia, P Moarefvan Aust J Basic Appl Sci 4 (2), 274-285 , 2010 2010.0 Citations: 9
Yield and yield components of wheat as influenced by sowing date and humic acid M Doroodian, Y Sharghi, A Alipour, H Zahedi International Journal of Natural Sciences 5 (1), 8-14 , 2015 2015.0 Citations: 8
Interaction effect of zeolite and salt stress in reproductive stage of four canola varieties V Esmaeilzadeh, H Zahedi, Y Sharghi, SAM Modarres Sanavy, ... Environmental Stresses in Crop Sciences 11 (2), 393-400 , 2018 2018.0 Citations: 7
Influence of vermicompost and bacterium of Bacillus and Pseudomonas on growth, yield and morphological traits of saffron. SM Farahani, S Amini, Y Sharghi, H Zahedi 2014.0 Citations: 7
A, Nourmohammadi G and Zahedi H. 2011. Yield and yield components of six canola (Brassica napus L.) cultivars affected by planting date and water deficit stress Y Sharghi, AH Shirani-Rad, B Ayeneh African Journal of Biotechnology 10 (46), 9309-9313 , 0 Citations: 7
Determination of Alluvium thickness in LeylanChai dam site using refraction seismic method S Rostami, Y Sharghi 18th Iranian geophysics conference, 482-485 , 2018 2018.0 Citations: 5
Effect of zeolite and calcium silicate on salt stress tolerance of two canola varieties. SS Ghoreishiasl, H Zahedi, Y Sharghi, S Sanavy, MM Ghahderijani 2017.0 Citations: 5
Effect of amino acid foliar application on morphophysiological characteristics and thyme essential oil under different irrigation regimes A Kazempour, Y Sharghi, SAM Modarres Sanavi, H Zahedi Journal of Plant Process and Function 12 (53), 71-90 , 2023 2023.0 Citations: 4
Effects of seed germination with gibberellin on safflower germination under salinity stress M Nejati, A Alipour, M Yousefirad, Y Sharghi, H Zahedi Iranian Journal of Plant Physiology 5 (1), 3443 , 2020 2020.0 Citations: 4
Effect of calcium silicate on salt stress tolerance of four canola varieties M Sanavi, SA Mohammad Iranian Journal of Field Crop Science 49 (1), 179-188 , 2018 2018.0 Citations: 4
Effects of different irrigation levels and phosphate fertilizer doses with or without nano iron application on sugar beet growth and physiology K Katal, A Alipour, H Zahedi, Y Sharghi, M Alavifazel Russian Journal of Plant Physiology 71 (6), 190 , 2024 2024.0 Citations: 3
Comparing effects of folic acid, epibrassinolide, chitosan and glutathione foliar treatments on safflower’s physiology and yield during water stress N Abdiazar, H Zahedi, Y Sharghi, SAM Modarres-Sanavy, A Alipour Russian Journal of Plant Physiology 71 (1), 35 , 2024 2024.0 Citations: 3