Dr. Devendra S. Shirode

Scopus Publications

In-vitro Anti-oxidant Studies and Preclinical Evaluation of the Hepatoprotective effect of Grewia asiatica in Paracetamol Induced Hepatotoxicity mice model
Jain Brijendra B, Shirode Devendra S, Pawar Vishakha S
Research Journal of Pharmacy and Technology, 2026
Hepatotoxicity can arise from various factors, including medication use (such as paracetamol and isoniazid), excessive alcohol consumption, viral infections (e.g., hepatitis), exposure to chemicals, genetic factors, autoimmune diseases, and pre-existing liver conditions. Notably, in cases of paracetamol overdose, the drug's metabolism shifts from safe conjugation pathways to oxidation by CYP450 enzymes, resulting in the formation of the toxic metabolite NAPQI. This metabolite depletes glutathione, leading to oxidative stress, lipid peroxidation, and mitochondrial dysfunction, which trigger cell death pathways and ultimately causing liver cell damage. Our research utilized an animal model to evaluate the hepatoprotective potential of the ethanolic extract of Grewia asiatica fruits against paracetamol-induced hepatotoxicity. The evaluation included various physical parameters (liver weight, body weight, and liver volume) and biochemical markers (AST, ALP, ALT, total proteins, albumin, globulin, and total bilirubin). Additionally, we assessed phenobarbitone-induced sleeping time and conducted histopathological examinations. Preliminary phytoconstituent analyses revealed that the ethanolic extract of Grewia asiatica (EEGA) contains a variety of compounds. In vitro antioxidant studies indicated significant antioxidant activity of the EEGA. In preventive studies, the extract at a dose of 1600 mg/kg exhibited notable hepatoprotective effects by decreasing levels of biochemical markers such as AST, ALT, ALP, and total bilirubin while increasing total proteins, albumin, and globulin. Treatment with EEGA at this dosage also resulted in a significant decrease in liver weight and a reduction in liver volume. The extract at both 800 mg/kg and 1600 mg/kg significantly elevated tissue glutathione (GSH) levels. Histopathological evaluations confirmed the hepatoprotective activity of the extract administered prior to paracetamol exposure. Furthermore, treatment with EEGA notably reduced phenobarbitone-induced sleeping times in paracetamol-treated animals in a dose-dependent manner, indicating the normalization of cytochrome P450 enzymes and further supporting the hepatoprotective properties of Grewia asiatica.
Preclinical Evaluation of Anti-hemorrhoidal Activity of L-carnitine L-tartrate (LCLT) via Stimulation of Anti-inflammatory and Antioxidant Activity in Croton Oil-induced Hemorrhoid Model
Avinash A. Gunjal, Ashish V. Kulkarni, Devendra S. Shirode, Prasad K. Bairagi, Vasant Y. Chavan, Niraj S. Vyawahare
Research Journal of Pharmacy and Technology, 2025
Background and Objectives: L-carnitine (LC) is essential for energy production and the breakdown of fatty acids in mammals, plants, and specific bacteria. LC is known for its anti-inflammatory and antioxidant activities. The primary goal of this investigation was to examine the potential of L-carnitine L-tartrate (LCLT) as a supplement in treating hemorrhoids by using the model of Croton oil-induced hemorrhoid. Materials and Methods: LCLT was procured from Sigma Aldrich, USA. Hemorrhoids were created in the anorectal region of the experimental rats using the Croton oil-induced hemorrhoid model. Subsequently, the rats were treated with LCLT (300 and 500 mg/kg) and a standard medication Pilex granules (400 mg/kg), for a period of 7 days. On the 7th day, after 1 hour of treatment anti-hemorrhoidal potential was evaluated by examining the severity score, Evans blue (EB) dye exudation, rectoanal coefficient (RAC), cytokines profiling, and histopathology of anorectal tissue. Results: Supplementation with LCLT significantly reduced the severity score, Evans blue exudation, recto-anal coefficient, and cytokines like PG-E2, TNF-α, IL-6, and MMP-3. Additionally, histopathological examination showed a marked reduction in inflammation, congestion, and mononuclear cell (MNC) infiltration in anorectal tissue. Conclusion: This study demonstrated that LCLT can be used as a supplement to treat hemorrhoids because of its anti-inflammatory and antioxidant activities.
Antiasthmatic Potential of Justicia gendarussa: A Preclinical Study
Pornima A. Sontakke, Niraj S. Vyawahare, Pavankumar P. Wankhade, Devendra S. Shirode, Ramdas D. Shinde, Vaishnavi P. Patil, Prasad K. Bairagi, Gayatri H. Patil
Journal of Natural Remedies, 2025
Background: The chronic respiratory lung illness known as asthma is reversible. Hyperresponsiveness and inflammation of the airways are characteristics of asthma. The pathogenesis of asthma is dominated by Th2 cell immune responses. Justicia gendarussa Burm. f (J. gendarussa) is an evergreen plant belonging to the Acanthaceae family. The presence of flavonoids and phenols gives the basis for the anti-inflammatory activity of the J. gendarussa. Traditionally in China, J. gendarussa is used to treat coughing diseases. Leaf ash was traditionally used to treat asthma in China. Aim: This study aims to assess the antiasthmatic potential of ethanolic extract of J. gendarussa leaves (EEJG) using in vitro and in vivo methods. Method: In this study, I have prepared J. gendarussa leaves extract by the Soxhlet method and done phytochemical analysis and an acute oral toxicity study. And Pharmacological screening was done by using an in vitro method that is DPPH assay and Goat trachea chain method, and in vivo leukocytosis and eosinophilia were caused by milk in Swiss albino mice, clonidine induced mast cell degranulation in Wistar rats and ovalbumen induced allergic asthma in Wistar rats. Result: The results indicated that the ethanolic extract of J. gendarussa drastically showed statistically significant inhibition of milk induced leukocytosis and eosinophilia in a dose-dependent manner, have mast cell stabilizing potential and also lowered BALF’s synthesis of Th2 cytokine (IL-4), as well as the numbers of major and total inflammatory cells. Histological investigations showed that significantly reduced the infiltration of inflammatory cells into lung tissues caused by OVA. Conclusion: J. gendarussa is beneficial for asthma therapy. The results demonstrated that J. gendarussa leaves extract exhibits significant antihistaminic, antiallergic, anti-inflammatory, and immunomodulatory actions in animal models used in experiments, successfully reducing asthma symptoms. These findings validate the traditional use of J. gendarussa and support the prospective therapeutic application of EEJG in the prevention and intervention of asthma. Major Findings: In animal models (rats and mice), the study showed that Justicia gendarussa had strong antiasthmatic activity. Plant extract treatment decreased bronchial hyperresponsiveness, eosinophil infiltration, and airway inflammation, indicating its promise as a natural therapeutic agent for asthma control.
Unraveling the Antiobesity Potential of Balanites aegyptiaca Leaves through Computational techniques
Dhanashri Sonavane, Devendra Shirode, Shubhangi Daswadkar, Gauri Gawande, Jaydip Bhapkar
Research Journal of Chemistry and Environment, 2025
Obesity is a major health problem worldwide caused by the excessive accumulation of body fat which is linked to cause various diseases such as diabetes, heart disease, cancer and others. In this study, we used computational techniques (network pharmacology and molecular docking) for Balanites aegyptiaca leaves (BAL) to treat obesity. Three active compounds were identified by using network pharmacology which are: Quercetin, Acacetin, Apigenin while obesity related targets genes were obtained from GeneCards database. These genes are involved in fat metabolism, energy balance and inflammation. Molecular docking showed strong binding affinity of these phytochemicals. AKT1 is the most significant target for obesity. The 3Dimentional structure of AKT1 protein (PDB ID: 3O96) was retrieved from protein data bank which is optimized and analyzed for its interactions. These findings suggest that Balanites aegyptiaca leaves may help in obesity management by targeting key biological pathways. However, further laboratory and clinical studies are needed to confirm effectiveness of Balanites aegyptiaca leaves in the treatment of obesity.
Network Pharmacology Tools used in Identification of Depression Targets
Devendra Shirode, Priyanka Patil
Research Journal of Biotechnology, 2025
A common and major psychological disorder called depression is characterized by persistent sadness and hopelessness as well as a lack of interest in or enjoyment from routine activities. Millions of people are impacted globally and it interferes with their capacity to go about their daily lives. Depression involves a complicated etiologic that includes genetic, metabolic, environmental and psychological components. Emotional dysregulation, cognitive decline, physical discomfort and behavioural abnormalities are the signs. A combination of medical treatment, psychotherapy and lifestyle changes is usually used in effective treatment. Comprehending the complex nature of depression is vital in order to develop more efficacious therapies and provide comprehensive support to people affected. Depression is difficult to treat since there is a wide range of symptoms, making diagnosis difficult. Furthermore, depression frequently co-occurs with other health conditions, making treatment more difficult and not everyone has access to mental health services. The branch of network pharmacology is an emerging field which integrates systems biology, bioinformatics and pharmacology to understand the complex interactions existing between targets, medications, protein-protein interactions, biological networks. Rather than focusing on individual drug-target interactions, network pharmacology takes into account several targets and pathways in order to offer a comprehensive understanding of drug action and disease mechanisms. By identifying complex biological networks, this method decreases side effects, estimates synergistic effects and makes it easier to identify possible multi-target medications. It has potential use in medication repurposing, personalized treatment and the creation of more potent therapeutic approaches.
Fractionation, HRLC-MS assisted phytochemical profiling, quantification, and evaluation of the chronic wound healing potential of ethanolic extract of Ardisia solanacea (Poir.) Roxb.
Mohini Tawade, Jyotsna Chopade, Asha Thomas, Devendra Shirode
Pharmacological Research Natural Products, 2025
Formulation, Evaluation and Acute Oral Toxicity Study of Polyherbal Syrup TCZoAP
Vaishnavi P. Patil, Pornima A. Sontakke, Manswi R. Deore, Gunjansing D. Rajput, Pavankumar P. Wankhade, Devendra S. Shirode, Niraj S. Vyawahare
Research Journal of Pharmacy and Technology, 2025
Polyherbalism is an ancient Ayurvedic concept for treating complex diseases. Nowadays, many CNS-associated complications are faced by people worldwide. Ayurvedic principles of polyherbalism were known for their effectiveness. In this study, a brain tonic was prepared as a polyherbal syrup formulation. Polyherbal syrup formulations were designed and evaluated. A total of five plants Zingiber officinale, Piper cubeba, Cinnamomum zeylanicum, Anacyclus pyrethrum, and Terminalia chebula were selected for formulation. These plants have already been used in many animal models for neuroprotective activity. After maceration, hydroalcoholic extracts were prepared and evaluated for phytochemical tests and IC50 values. The formulation was prepared and evaluated for various parameters like physical appearance, pH, density, viscosity, specific gravity, clarity test, and FTIR analysis of active phytoconstituents. After confirmation of quality control parameters, we performed acute oral toxicity (423), according to OECD guidelines. Based on this evaluation, we conclude that this polyherbal syrup formulation will be used in the future for preclinical evaluation of neuroprotection in various animal models to check pharmacological effects.
Targeting the molecular web of Alzheimer’s disease: unveiling pathways for effective pharmacotherapy
Devika Jadhav, Nikita Saraswat, Neeraj Vyawahare, Devendra Shirode
Egyptian Journal of Neurology Psychiatry and Neurosurgery, 2024
Introduction Alzheimer’s disease is a neurocognitive disorder that affects elderly people by slowly impaired cognition, dementia, and gets worse with age. It slowly impacts the quality of life. Clinically, it is distinguished by a transition from episodic memory to a gradual reduction in cognitive ability leading to cognitive dysfunction. Neurofibrillary tangles and amyloid plaques are unique structures that are thought to have a role in the pathogenesis of Alzheimer's disease. In this review, we focus our attention on the risk factors, pathophysiology, etiology, epidemiology, stages, diagnosis, treatment, mechanisms, pathways, ongoing clinical trials data and risks potentially associated with the development of Alzheimer's disease. Short summary This review aims to extrapolate the information about Alzheimer's disease. Preliminary research was done by selecting reviews on PubMed, Elsevier, and Google open-access publications using the keywords like “Alzheimer, dementia, neurodegenerative, memory, amyloid β, mechanism of action, pathways”. Conclusion Here we show the discussion and interpretation of several signaling pathways in the pathogenesis of Alzheimer's disease such as amyloid β plaque cleavage, Metal ion hypothesis, amyloid β degradation, initiation of amyloidogenic and non-amyloidogenic pathway, oxidative stress hypothesis, Metabolic syndrome, insulin resistance and tau phosphorylation associated apolipoprotein- cholesterol, neurofibrillary tangles accumulation, and insulin resistance which are significant for better understanding of the disease initiation and progression. On studying the ongoing clinical trials, it was found that current drugs being tested are crenezumab, gantenerumab and sodium oligonucleotide. Graphical Abstract
Pre-clinical evaluation of neuroprotective effect of Albizzia lebbeck bark extract in Cuprizone induced Multiple sclerosis
Devendra S. Shirode, Ashwini M. Gavit
Research Journal of Biotechnology, 2024
Multiple sclerosis (MS) is a complex and debilitating autoimmune disease of the central nervous system (CNS), affecting millions of individuals worldwide. Despite significant advances in the understanding of its pathogenesis, an effective cure for MS remains elusive. Current treatments are often associated with adverse side effects and limited long-term efficacy. Therefore, there is a growing need for novel therapeutic strategies that can offer both neuroprotection and remyelination. Herbal medicine has emerged as a promising avenue in this regard, with various natural extracts showing potential in preclinical MS models. Among these, Albizia lebbeck bark extract has garnered attention due to its reputed anti-inflammatory and neuroprotective properties. In this research study, we explore the preclinical evaluation of Albizia lebbeck bark extract in cuprizone-induced MS, aiming to shed light on its therapeutic potential and possible mechanisms of action.
Computational tools to predict drug release kinetics in solid oral dosage forms
Software and Programming Tools in Pharmaceutical Research, 2024
Effect of Niosomal Encapsulation of Quercetin and Silymarin and their Combination on Dimethylnitrosoamine-induced and Phenobarbital-promoted Hepatocellular Carcinoma in Rat Model
Devendra S. Shirode, Dinesh J. Raut, Nikita Sarasawat
Current Drug Discovery Technologies, 2024
Anti-inflammatory effect of Polyherbal Formulation
Aishwarya S. Patil, Mayuri A. Tupe, Devendra S. Shirode
Research Journal of Pharmacy and Technology, 2024
Antiulcer effect of blumea lacera against Gastric ulcers in rats
Devendra S. Shirode, Priyatama Powar, Brijendra B. Jain, Amit Agarwal
Research Journal of Pharmacy and Technology, 2020
Effect of ethanol extract of Hugonia mystax on Rifampicin induced hepatotoxicity in rats
Journal of Chemical and Pharmaceutical Sciences, 2015
In vivo anti-oxidant and hepatoprotective effects of hugonia mystax in paracetamol induced hepatotoxicity in rats
Asian Journal of Pharmaceutical and Clinical Research, 2015
Efficacy of sphaeranthus indicus in the treatment of rifampicin induced hepatotoxicity in rats
Devendra S. Shirode, Brijendra B. Jain, C.B. Mahendra Kumar
Biosciences Biotechnology Research Asia, 2015
Hepatoprotective and antioxidant effects of Albizzia lebbeck against thioacetamide induced hepatotoxicity in rats
Journal of Chemical and Pharmaceutical Sciences, 2012
Evaluation of antioxidant and hepatoprotective effects of 70% ethanolic bark extract of Albizzia lebbeck in rats
International Journal of Research in Pharmaceutical Sciences, 2010
In vivo anticancer activity of Clerodendrum serratum (L) moon.
Research Journal of Pharmaceutical Biological and Chemical Sciences, 2010
Antioxidant and hepatoprotective activity of Madhuca longifolia (koenig) bark against CCl4 - induced hepatic injury in rats: In vitro and In vivo studies
Research Journal of Pharmaceutical Biological and Chemical Sciences, 2010
Influence of metronidazole on hypoglycaemic activity of thiazolidinediones in normal and alloxan induced diabetic rats
Indian Journal of Pharmaceutical Education and Research, 2009
Anti-ulcer properties of 70% ethanolic extract of leaves of Albizzia lebbeck
Pharmacognosy Magazine, 2008
Comparative antimicrobial studies of ethanolic extract of leaves of Nerium indicum & Martynia annua
Biosciences Biotechnology Research Asia, 2006
Study of the analgesic properties of ethanolic extract of leaves of Nerium indicum Mill
Biosciences Biotechnology Research Asia, 2006
Hair growth activity of Hibiscus rosa-sinensis and Glycyrrhiza glabra
Biosciences Biotechnology Research Asia, 2005

Dr. Devendra S. Shirode

RESEARCH INTERESTS

Scopus Publications