Vanita Somasekhar

@klepharmblr.org

Professor and Head, Department of Pharmaceutical Analysis
KLE College of Pharmacy, Bengaluru

Vanita Somasekhar


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https://researchid.co/vanitasom

EDUCATION

M.Pharm., Ph.D.

RESEARCH INTERESTS

Instrumental analysis
19

Scopus Publications

100

Scholar Citations

5

Scholar h-index

3

Scholar i10-index

Scopus Publications

  • Formulation and evaluation of isosorbide dinitrate-loaded flash-release dispersible sublingual wafers
    Shivakumar H. Nanjappa, Vanita Somasekhar, Megha Kamath, Sanjana S. Prakash, Avichal Kumar, Raksha Pai, Rushikesh Shinde
    Rsc Pharmaceutics, 2026
    This study aimed to design flash-release dispersible sublingual wafers of isosorbide dinitrate (ISDN) by lyophilisation (LYO) by applying the principles of Quality by Design (QbD).
  • Activation-modulated stimuli-responsive systems: an intelligent platform for site-specific gastroretentive delivery of diverse therapeutic agents
    H.N. Shivakumar, Rushikesh Shinde, Vanita Somasekhar, M.G. Hariprasad, N.M. Mahesh
    International Journal of Pharmaceutics, 2026
  • Shilajit-analytical study to understand the phyto complex present in shilajit raw material, extract and resin by using hyphenated techniques
    Harsimran Kaur Kohli, Vanita Somasekhar, M. Devendra Reddy, N.S. Prakash
    Journal of Chromatography A, 2026
  • A Comprehensive Analysis of Andrographis paniculata Herbal Products Conducted to Enhance Potency and Ensure Safety Through the Application of Pertinent Chromatographic Techniques.
    Tamilvanan K, P.V Murali Krishna, Vanita Somasekhar, Rajeshkumar R
    Research Journal of Pharmacy and Technology, 2026
    The study aimed to identify and quantify andrographolides in various marketed samples of A. Paniculata and detect pesticide residues. It aimed to improve understanding of the composition, purit, and safety.The TLC technique used an aluminium plate with pre-coated silica gel (E. Merck – Silica Gel 60F254). Samples were spotted using a Linomat IV syringe TLC applicator (CAMAG) and a solvent system consisting of acetone, benzene, and chloroform (20:20:10 ratio). With a LiChrospher 100 RP-18e (5μm) column, a quaternary pump, UV detector, auto injector, column oven, and LC-Solution software, the HPLC system comprised a SHIMADZU LC-2030C Liquid Chromatograph. The Agilent Gas Chromatograph with Electron Capture and Nitrogen Phosphorus Detector 7890A-ECD/NPD (EZ Chrome) and associated equipment, along with a Zebron ZB-1 column (30 m in length, 0.25 mm in diameter, and 0.50 m film thickness), made up the GC-ECD system.The TLC results showed well-developed andrographolide spots in all samples, with sample C having the highest percentage. HPLC quantification confirmed this, with sample C exhibiting the highest total andrographolide content. GC-ECD analysis detected pesticide residues in A. paniculata samples. This comprehensive chromatographic analysis enhances our understanding of A. paniculata composition and purity, ensuring potency and safety in herbal products. These findings provide valuable insights for quality control in A. paniculata-based formulations.Our research emphasises the safety and therapeutic potential of Andrographis paniculata, underscoring its importance in contemporary medicine. By using cutting-edge methods for thorough study, we were able to verify its effectiveness against several diseases.
  • An AQbD driven stability indicating HPLC method for simultaneous estimation of lamivudine, tenofovir disoproxil fumarate and efavirenz in plasma
    Avichal Kumar, Vanita Somasekhar, Sumit Dhiman, Shivakumar Hagalavadi Nanjappa, Dhruti Avlani
    Analytical Methods, 2025
    Advanced AQbD involving D-optimal and Box–Behnken designs for precise analysis of fixed dose combination of antiretrovirals in plasma.
  • Gymnema sylvestre saponins: Analytical profiling for herbal pharmaceutical standards
    Vanita Somasekhar, Raksha Pai, Prakash NS, Devendra Reddy M
    Journal of Liquid Chromatography and Related Technologies, 2025
    Background Gymnema sylvestre is renowned for its antidiabetic and antisweet properties, attributed to triterpenoid saponins such as gymnemic acids and gymnemagenin. The objective of this study was to develop a novel and robust method for the extraction, preparative-scale isolation, and LC–MS/MS-based characterization of these saponins to support the standardization of G. sylvestre-based herbal formulations.Methods Methanol, ethanol, and aqueous extractions were evaluated, with methanol yielding the highest total gymnemic acids (4.69% w/w). Advanced analytical techniques including HPTLC, HPLC, and preparative HPLC were employed for separation and isolation. LC-MS/MS analysis was used for structural identification based on retention time and mass fragmentation.Results Three major saponins were successfully isolated and identified: deacyl gymnemic acid (m/z 680.9 [M–H]−; MW 682.8 g/mol), gymnemagenin (m/z 505 [M–H]−; MW 506.7 g/mol), and gymnemic acid IV (m/z 762.9 [M–H]−; MW 764.9 g/mol). The method was highly reproducible and suitable for both qualitative and quantitative applications.Conclusion This study presents a novel, comprehensive analytical strategy for the isolation and characterization of Gymnema saponins. The validated method enables use of these compounds as reference markers, supporting quality control and regulatory compliance in herbal pharmaceutical development.
  • DEVELOPMENT OF A DISCRIMINATORY METHOD FOR DETERMINATION OF IN VITRO DISSOLUTION OF CILNIDIPINE
    Vanita Somasekhar, Tejashwini H, Murali krishna PV
    Asian Journal of Pharmaceutical and Clinical Research, 2024
    Objectives: This study involves the development of a discriminatory method to differentiate the in vitro dissolution of cilnidipine’s mouth-dispersing film from that of a commercial tablet. Methods: The mouth-dispersing film was initially developed using a reported established method. A discriminatory dissolution method was developed to assess the in vitro dissolution of the mouth-dispersing film and compare the same with that of the commercial tablet. Results: The study findings indicated the method employed was able to differentiate the dissolution of the fast-dissolving film from the commercial tablet. About 98.52±0.60% of the drug was found to be released from the oral film in 40 min whereas the tablet took nearly 5 h for 92.06±0.49% of the drug to get released. Conclusion: These results demonstrate that the dissolution media identified was able to create the necessary sink condition that would be able to differentiate the dissolution profile of the mouth-dispersing film from the conventional tablet. The ultrasound spectrophotometric method employed was found to be simple rapid and sensitive enough to estimate the drug release from diverse pharmaceutical dosage forms.
  • Development and Validation of High-Performance Thin Layer Chromatographic Method for Estimation of Acyclovir
    Pai Raksha, Somasekhar Vanita, Purushottaman Vijayanthimala, Chandran Athira
    Indian Journal of Pharmaceutical Education and Research, 2024
    Background: The antiviral medication genre comprises the synthetic purine nucleoside analogue acyclovir, which particularly is used to treat varicella-zoster and herpes simplex virus infections. By incorporating into viral DNA to stop further synthesis, acyclovir limits DNA synthesis and viral multiplication. The investigation of an economical alternative was spurred by the existence of the currently used HPLC and UV techniques for acyclovir analysis, which are recognized for their solvent-intensive character. The innovation offers a more cost-effective method of pharmaceutical analysis along with improving sustainability. Materials and Methods: Using a CAMAG Linomat 5 sampler, the samples were spotted in the form of bands on a precoated silica gel plate using a CAMAG microliter syringe. The application rate was kept constant at 100 nL/sec and the spacing between the tracks was set. Chloroform, ethanol, isopropyl alcohol and strong ammonia (2:4:3:1 v/v/v/v) constitute the mobile phase. The development process was linear ascending in a twin trough glass chamber that was saturated with a mobile phase. To estimate acyclovir, densitometric scanning was carried out in the absorbance mode at 254 nm. Results: With r 2 =0.9974, the calibration plots' linear regression analysis results demonstrated a strong linear association. The limit of quantification was 11.7726 µg/mL, whereas the limit of detection was 3.884 µg/mL. Conclusion: According to statistical analysis of the data, the approach was found to be precise, accurate, repeatable, sensitive and selective for the analysis of acyclovir. The technique will work well for routine quality control when estimating acyclovir as a bulk medication.
  • RP-HPLC Method Development and Validation of Choline Salicylate and Lignocaine HCl in Mouth Ulcer Gel
    Sivanathan Boopalan, Palla Venkata Murali Krishna, Vanita Somasekhar, Venketesh Dubalgundi, Shruthi Devadiga
    Indian Journal of Pharmaceutical Education and Research, 2024
    Objectives The prime intent of the current study is to develop a rapid, reliable, robust and cost-effective reversed-phase HPLC method for simultaneous estimation of Choline Salicylate and Lignocaine HCl in mouth Ulcer Gel. Materials and Methods Buffer was prepared with 3.5 g of disodium hydrogen orthophosphate in 1000 mL of water and adjusted to pH 4.5 with dilute orthophosphoric acid. A mixture of Buffer and Methanol (55:45) containing 0.01M (2.062 g) 1-Hexane Sulphonic acid comprised the mobile phase. The separation was carried out with a Hypersil C18 column (250x4.6 mm, 5 μ) maintained at a temperature of 30°C at a flow rate of 1.0 mL/min with detection at 220 nm. The Injection volume was 20 μL and the run time was 20 min. Results Retention time for choline salicylate and Lignocaine HCl was 3.163 and 9.629 min respectively. Linearity for Choline Salicylate and Lignocaine Hydrochloride was found to be in the range of 12.5-50 μg/mL. The correlation coefficient for the linear curve obtained between concentration vs. area for standard preparations of Choline Salicylate and Lignocaine HCl was 0.9992 and 0.9993 respectively. The projected procedure successfully satisfied the specificity and robustness parameters. Conclusion The analytical method was successfully validated according to ICH guidelines (ICH, Q2 (R1)). The proposed novel method is reliable and cost-effective and is well-suited for use in the pharmaceutical industry.
  • Preparation and characterization of directly compressible spherical agglomerates of etoricoxib
    Hagalavadi Nanjappa Shivakumar, Vanita Somasekhar, Anwesha Roy
    Indian Journal of Pharmaceutical Education and Research, 2020
    The current work contemplates formulation of spherical agglomerates of Etoricoxib, with a view to improve the flow properties, solubility and dissolution rate. Materials and Methods: Spherical agglomerates of etoricoxib were produced employing quasi emulsion solvent diffusion method using hydrophilic polymers like PVP K30 and PEG 6000. IR spectroscopy and Scanning Electron Microscopy along with physicochemical evaluations were used to characterize the agglomerates. Results: The optical microscopic studies indicated that the agglomerates were spherical in shape and varied in size from 1203.9 126m to 1297.2 164.2m. The technique was found to enhance the aqueous solubility of the drug from 1.45 g/ml to 8.260.05 g/ml and the dissolution in 60 min from 39.57% to 82.88%. Etoricoxib tablets prepared using spherical agglomerates with PVP K30 and PEG 6000 displayed dissolution of 82.88% and 76.89% respectively that were considerably higher than conventional etoricoxib tablets. Conclusion: From the dissolution profile and % cumulative release from formulations it was concluded that Spherical Agglomeration technique substantially improves the flow properties, aqueous solubility and rate of dissolution of etoricoxib.
  • Comparative antioxidant and bioavailability studies of vitamin C in Phyllanthus emblica Linn. And its combinations with piper nigrum Linn. and Zingiber officinale Roscoe
    Vanita Somasekhar, Purnima Ashok, Sri Adibatla Renuka Kameswari, Ramaswamy Rajendran, Rajpreet Singh
    Brazilian Journal of Pharmaceutical Sciences, 2016
  • Optimization and validation of an RP-HPLC method for the estimation of 6-mercaptopurine in bulk and pharmaceutical formulations
    Vanita Somasekhar
    Brazilian Journal of Pharmaceutical Sciences, 2014
  • HPLC method for the simultaneous determination of paracetamol, aceclofenac and tizanidine hydrochloride
    Asian Journal of Chemistry, 2011
  • Development and validation of a rapid RP-HPLC method for the estimation of esmolol hydrochloride in bulk and pharmaceutical dosage forms
    Vanita Somasekhar, D. Gowri Sankar
    E Journal of Chemistry, 2010
  • Development and validation of a rapid RP-HPLC method for the determination of venlafaxine hydrochloride in pharmaceutical dosage forms using experimental design
    Vanita Somasekhar, Dannana Gowrisankar, H. N. Shivakumar
    E Journal of Chemistry, 2009
  • Screening of venlafaxine hydrochloride for transdermal delivery: Passive diffusion and iontophoresis
    Gursharanjit Singh, Bijaya Ghosh, Dave Kaushalkumar, Vanita Somsekhar
    AAPS Pharmscitech, 2008
  • Synthesis of 2-N-acylamino-4-arylthiazole-5-acetic acids/esters for their anti-inflammatory and analgesic activities
    Asian Journal of Chemistry, 2004
  • An unexpected reaction in the synthesis of 3-substituted thienoazetidinones
    Asian Journal of Chemistry, 2003
  • An oxidative spectrophotometric method for the determination of clprofloxacln in pharmaceutical preparations
    Indian Drugs, 1997

RECENT SCHOLAR PUBLICATIONS

  • Activation-modulated stimuli-responsive systems: an intelligent platform for site-specific gastroretentive delivery of diverse therapeutic agents
    HN Shivakumar, R Shinde, V Somasekhar, MG Hariprasad, NM Mahesh
    International Journal of Pharmaceutics, 126599 , 2026
    2026
  • Formulation and evaluation of isosorbide dinitrate-loaded flash-release dispersible sublingual wafers
    SH Nanjappa, V Somasekhar, M Kamath, SS Prakash, A Kumar, R Pai, ...
    RSC Pharmaceutics , 2026
    2026
  • Gymnema sylvestre saponins: Analytical profiling for herbal pharmaceutical standards
    V Somasekhar, R Pai, P Ns, D Reddy M
    Journal of Liquid Chromatography & Related Technologies 48 (16-20), 558-564 , 2025
    2025
  • Shilajit-analytical study to understand the phyto complex present in shilajit raw material, extract and resin by using hyphenated techniques
    HK Kohli, V Somasekhar, MD Reddy, NS Prakash
    Journal of Chromatography A, 466570 , 2025
    2025
  • An AQbD driven stability indicating HPLC method for simultaneous estimation of lamivudine, tenofovir disoproxil fumarate and efavirenz in plasma
    A Kumar, V Somasekhar, S Dhiman, SH Nanjappa, D Avlani
    Analytical Methods 17 (9), 2094-2111 , 2025
    2025
    Citations: 2
  • Development and Validation of High-Performance Thin Layer Chromatographic Method for Estimation of Acyclovir.
    P Raksha, S Vanita, P Vijayanthimala, C Athira
    Indian Journal of Pharmaceutical Education & Research 58 , 2024
    2024
  • RP-HPLC Method Development and Validation of Choline Salicylate and Lignocaine HCl in Mouth Ulcer Gel.
    S Boopalan, PVM Krishna, V Somasekhar, V Dubalgundi, S Devadiga
    Indian Journal of Pharmaceutical Education & Research 58 , 2024
    2024
    Citations: 1
  • DEVELOPMENT OF A DISCRIMINATORY METHOD FOR DETERMINATION OF IN VITRO DISSOLUTION OF CILNIDIPINE
    V SOMASEKHAR, H TEJASHWINI, MK PV
    DEVELOPMENT 17 (2) , 2024
    2024
  • Preparation and Characterization of Directly Compressible Spherical Agglomerates of Etoricoxib.
    HN Shivakumar, V Somasekhar, A Roy
    Indian Journal of Pharmaceutical Education & Research 54 (4) , 2020
    2020
    Citations: 3
  • SYNTHESIS AND EVALUATION OF ANTI-PROLIFERATIVE ACTIVITY OF NOVEL THIAZOLIDINONE DERIVATIVES
    V Somasekhar, R Udainiya, S Kumar
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH 9 (8), 3449-3453 , 2018
    2018
  • Comparative antioxidant and bioavailability studies of Vitamin C in Phyllanthus emblica Linn. and its combinations with Piper nigrum Linn. and Zingiber officinale Roscoe
    V Somasekhar, P Ashok, SAR Kameswari, R Rajendran, R Singh
    Brazilian Journal of Pharmaceutical Sciences 52, 35-43 , 2016
    2016
    Citations: 13
  • Optimization and validation of an RP-HPLC method for the estimation of 6-mercaptopurine in bulk and pharmaceutical formulations
    V Somasekhar
    Brazilian Journal of Pharmaceutical Sciences 50, 793-797 , 2014
    2014
    Citations: 8
  • HPLC Method for the simultaneous determination of paracetamol, aceclofenac and tizanidine hydrochloride
    V Somasekhar, D Gowrisankar
    Asian Journal of Research in Chemistry 23 (4), 1651-1654 , 2011
    2011
    Citations: 9
  • Development and validation of an RP-HPLC method for estimation of famciclovir in bulk and pharmaceutical formulations
    V Somasekhar, D Gowrisankar
    Current Pharma Research 1 (3), 227-231 , 2011
    2011
    Citations: 2
  • Isolation, characterization and pharmacological studies of a flavonol glucoside from trichilia connaroides (w. &a.) Bentilizen
    D Meharoon
    KLE University, Belagavi, Karnataka , 2011
    2011
  • Development and Validation of a Rapid RP‐HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms
    V Somasekhar, DG Sankar
    Journal of Chemistry 7 (3), 807-812 , 2010
    2010
    Citations: 5
  • Development and Validation of a Rapid RP‐HPLC Method for the Determination of Venlafaxine Hydrochloride in Pharmaceutical Dosage forms using Experimental Design
    V Somasekhar, D Gowrisankar, HN Shivakumar
    Journal of Chemistry 6 (4), 1091-1102 , 2009
    2009
    Citations: 23
  • Screening of Venlafaxine Hydrochloride for Transdermal Delivery : Passive Diffusion and Iontophoresis
    VS 7. Gurusharanjit Sigh, Bijaya Ghosh, Dave Kaushalkumar D
    AAPSPharmSciTech 9 (3), 791-797 , 2008
    2008
    Citations: 31
  • Synthesis of 2-N-Acylamino-4-Arylthiazole-5-Acetic acids/esters for Their Anti-inflammatory and Analgesic Activities
    M Attimarad, V Somasekhar, S Mohan
    Asian Journal of Chemistry 16 (1), 179 , 2004
    2004
    Citations: 1
  • An unexpected reaction in the synthesis of 3-substituted thienoazetidinones
    J Saravanan, V Somasekhar, S Mohan
    Asian Journal of Chemistry 15 (3), 1749 , 2003
    2003
    Citations: 2

MOST CITED SCHOLAR PUBLICATIONS

  • Screening of Venlafaxine Hydrochloride for Transdermal Delivery : Passive Diffusion and Iontophoresis
    VS 7. Gurusharanjit Sigh, Bijaya Ghosh, Dave Kaushalkumar D
    AAPSPharmSciTech 9 (3), 791-797 , 2008
    2008
    Citations: 31
  • Development and Validation of a Rapid RP‐HPLC Method for the Determination of Venlafaxine Hydrochloride in Pharmaceutical Dosage forms using Experimental Design
    V Somasekhar, D Gowrisankar, HN Shivakumar
    Journal of Chemistry 6 (4), 1091-1102 , 2009
    2009
    Citations: 23
  • Comparative antioxidant and bioavailability studies of Vitamin C in Phyllanthus emblica Linn. and its combinations with Piper nigrum Linn. and Zingiber officinale Roscoe
    V Somasekhar, P Ashok, SAR Kameswari, R Rajendran, R Singh
    Brazilian Journal of Pharmaceutical Sciences 52, 35-43 , 2016
    2016
    Citations: 13
  • HPLC Method for the simultaneous determination of paracetamol, aceclofenac and tizanidine hydrochloride
    V Somasekhar, D Gowrisankar
    Asian Journal of Research in Chemistry 23 (4), 1651-1654 , 2011
    2011
    Citations: 9
  • Optimization and validation of an RP-HPLC method for the estimation of 6-mercaptopurine in bulk and pharmaceutical formulations
    V Somasekhar
    Brazilian Journal of Pharmaceutical Sciences 50, 793-797 , 2014
    2014
    Citations: 8
  • Development and Validation of a Rapid RP‐HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms
    V Somasekhar, DG Sankar
    Journal of Chemistry 7 (3), 807-812 , 2010
    2010
    Citations: 5
  • Preparation and Characterization of Directly Compressible Spherical Agglomerates of Etoricoxib.
    HN Shivakumar, V Somasekhar, A Roy
    Indian Journal of Pharmaceutical Education & Research 54 (4) , 2020
    2020
    Citations: 3
  • An AQbD driven stability indicating HPLC method for simultaneous estimation of lamivudine, tenofovir disoproxil fumarate and efavirenz in plasma
    A Kumar, V Somasekhar, S Dhiman, SH Nanjappa, D Avlani
    Analytical Methods 17 (9), 2094-2111 , 2025
    2025
    Citations: 2
  • Development and validation of an RP-HPLC method for estimation of famciclovir in bulk and pharmaceutical formulations
    V Somasekhar, D Gowrisankar
    Current Pharma Research 1 (3), 227-231 , 2011
    2011
    Citations: 2
  • An unexpected reaction in the synthesis of 3-substituted thienoazetidinones
    J Saravanan, V Somasekhar, S Mohan
    Asian Journal of Chemistry 15 (3), 1749 , 2003
    2003
    Citations: 2
  • RP-HPLC Method Development and Validation of Choline Salicylate and Lignocaine HCl in Mouth Ulcer Gel.
    S Boopalan, PVM Krishna, V Somasekhar, V Dubalgundi, S Devadiga
    Indian Journal of Pharmaceutical Education & Research 58 , 2024
    2024
    Citations: 1
  • Synthesis of 2-N-Acylamino-4-Arylthiazole-5-Acetic acids/esters for Their Anti-inflammatory and Analgesic Activities
    M Attimarad, V Somasekhar, S Mohan
    Asian Journal of Chemistry 16 (1), 179 , 2004
    2004
    Citations: 1
  • Activation-modulated stimuli-responsive systems: an intelligent platform for site-specific gastroretentive delivery of diverse therapeutic agents
    HN Shivakumar, R Shinde, V Somasekhar, MG Hariprasad, NM Mahesh
    International Journal of Pharmaceutics, 126599 , 2026
    2026
  • Formulation and evaluation of isosorbide dinitrate-loaded flash-release dispersible sublingual wafers
    SH Nanjappa, V Somasekhar, M Kamath, SS Prakash, A Kumar, R Pai, ...
    RSC Pharmaceutics , 2026
    2026
  • Gymnema sylvestre saponins: Analytical profiling for herbal pharmaceutical standards
    V Somasekhar, R Pai, P Ns, D Reddy M
    Journal of Liquid Chromatography & Related Technologies 48 (16-20), 558-564 , 2025
    2025
  • Shilajit-analytical study to understand the phyto complex present in shilajit raw material, extract and resin by using hyphenated techniques
    HK Kohli, V Somasekhar, MD Reddy, NS Prakash
    Journal of Chromatography A, 466570 , 2025
    2025
  • Development and Validation of High-Performance Thin Layer Chromatographic Method for Estimation of Acyclovir.
    P Raksha, S Vanita, P Vijayanthimala, C Athira
    Indian Journal of Pharmaceutical Education & Research 58 , 2024
    2024
  • DEVELOPMENT OF A DISCRIMINATORY METHOD FOR DETERMINATION OF IN VITRO DISSOLUTION OF CILNIDIPINE
    V SOMASEKHAR, H TEJASHWINI, MK PV
    DEVELOPMENT 17 (2) , 2024
    2024
  • SYNTHESIS AND EVALUATION OF ANTI-PROLIFERATIVE ACTIVITY OF NOVEL THIAZOLIDINONE DERIVATIVES
    V Somasekhar, R Udainiya, S Kumar
    INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH 9 (8), 3449-3453 , 2018
    2018
  • Isolation, characterization and pharmacological studies of a flavonol glucoside from trichilia connaroides (w. &a.) Bentilizen
    D Meharoon
    KLE University, Belagavi, Karnataka , 2011
    2011