Dr. Prasad Deepak Dandekar
@mitbio.edu.in
School of Bioengineering Sciences and Research, MITADT University
Assistant Professor
RESEARCH INTERESTS
Antidiabetics, Antiviral Drugs, Phytochemistry
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Prasad D. Dandekar, Amol S. Kotmale, Shrawan R. Chavan, Pranita P. Kadlag, Sangeeta V. Sawant, Dilip D. Dhavale, and Ameeta RaviKumar
American Chemical Society (ACS)
Use of human pancreatic α-amylase (HPA) inhibitors is one of the effective antidiabetic strategies to lower postprandial hyperglycemia via reduction in the dietary starch hydrolysis rate. Many natural products from plants are being studied for their HPA inhibitory activity. The present study describes isolation of dehydrodieugenol B (DDEB) from Ocimum tenuiflorum leaves using sequential solvent extraction, structure determination by one-dimensional (1D) and two-dimensional (2D) NMR analyses, and characterization as an HPA inhibitor using kinetics, binding thermodynamics, and molecular docking. DDEB uncompetitively inhibited HPA with an IC50 value of 29.6 μM for starch and apparent Ki′ of 2.49 and Ki of 47.6 μM for starch and maltopentaose as substrates, respectively. The circular dichroism (CD) study indicated structural changes in HPA on inhibitor binding. Isothermal titration calorimetry (ITC) revealed thermodynamically favorable binding (ΔG of −7.79 kcal mol–1) with a dissociation constant (Kd) of 1.97 μM and calculated association constant (Ka) of 0.507 μM. Molecular docking showed stable HPA–inhibitor binding involving H-bonds and Pi-alkyl, alkyl–alkyl, and van der Waals (vDW) interactions. The computational docking results support the noncompetitive nature of DDEB binding. The present study could be helpful for exploration of the molecule as a potential antidiabetic drug candidate to control postprandial hyperglycemia.
Prasad Dandekar, Sudha Ramkumar, and Ameeta RaviKumar
Elsevier