PREVALENCE AND BIOFILM FORMATION OF STAPHYLOCOCCUS SPP. FROM SOME OF DIARY PRODUCTS Veterinary Practitioner, 2022
Identification and phylogenetic study of Klebsiella pneumoniae VIA molecular-based techniques targeting 16S rRNA, magA, and rmpA in camels from Al-Diwaniyah city, Iraq Basima Jasim Mohammed, Zeena Foaad Saleh, Jameela Radi Esmaeel, Saba Falah Klaif, Mohammad Sabri Jawad, et al. Annals of Tropical Medicine and Public Health, 2020 This study was as an investigation to identify the presence of Klebsiella pneumoniae in the livers and lungs of camels in Al-diwaniyah City, Iraq. Here, 50 specimens were sampled from each organ of 50 camels. In the beginning, regular cultivation and biochemical tests were performed for primary detection. Then,a multiplex polymerase chain reaction (mPCR) targeting specific pieces of 16S rRNA, mucoviscosity-associated A (magA), and regulator of mucoid phenotype A (rmpA) genes was done for more confirmative identification of the bacterium. After that, the PCR-based products of the 16S rRNA gene were subjected to partial sequencing in which the results were used to generate a phylogenetic tree to compare our isolates with some previously identified strains of the K. pneumoniae. The results of the primary detection showed the presence of this bacterium in 30 (60%) and 42 (84%) of the liver and lung samples respectively. The mPCR results revealed the amplification of the tested genes at 30(100%)16S rRNA,25 (83%) magA, and 0 (0%) rmpA in the liver samples and 42(100%) 16S rRNA, 35 (92%) magA,and 30 (78.9%) rmpA in the samples of lungs. The sequencing results identified 2 isolates (MH119051.1) and (MH119052.1) which gave close matching with some global stains such asKM203760.1, an Indian isolate. Our study gives valued information about the presence of this bacterium that causes infection to the camels in the studied city.
Molecular identification of Brucella abortus and its virulence genes (bvfA, virB, and ure) in infected humans and cattle from Al-Diwaniyah province, Iraq Jameela Radi Esmaeel Annals of Tropical Medicine and Public Health, 2019 Materials and methods: According to that aim, 35 human-blood and 50 cattle-milk samples were collected from Al-Diwaniyah Teaching Hospital and local herd fields, respectively, from Al-Diwaniyah province, Iraq. A polymerase chain reaction (PCR) technique was employed to determine the identity of the bacterium and the presence of its VGs in the samples. Moreover, a partial gene sequencing (PGS) method targeted the 16S rRNA gene was used to confirm the infection by B. abortus in the samples.
RECENT SCHOLAR PUBLICATIONS
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Identification and phylogenetic study of Klebsiella pneumoniae via molecular-based techniques targeting 16S rRNA, magA, and rmpA in camels from Al-Diwaniyah city, Iraq BJ Mohammed, ZF Saleh, JR Esmaeel, SF Klaif, MS Jawad, KH Fahad Annals of Tropical Medicine and Health 23 (5), 218-225 , 2020 2020.0 Citations: 11
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MOST CITED SCHOLAR PUBLICATIONS
Identification and phylogenetic study of Klebsiella pneumoniae via molecular-based techniques targeting 16S rRNA, magA, and rmpA in camels from Al-Diwaniyah city, Iraq BJ Mohammed, ZF Saleh, JR Esmaeel, SF Klaif, MS Jawad, KH Fahad Annals of Tropical Medicine and Health 23 (5), 218-225 , 2020 2020.0 Citations: 11
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Direct detection of Clostridium perfringens based alpha toxin gene from sheep and cattle by real time PCR techique BM Mizher, JN Sadeq, JR Esmaeel Basrah Journal of Veterinary Research 15 (2), 1-9 , 2016 2016.0 Citations: 4
Molecular identification of Brucella abortus and its virulence genes (bvfA, virB, and ure) in infected humans and cattle from Al-Diwaniyah province, Iraq JR Esmaeel Annals of Tropical Medicine & Public Health 164 , 2019 2019.0 Citations: 3
Detection virulence factors of Klebsiella pneumonia from cattle by using PCR tec. JR Esmaeel, JN Sadeq Kufa Journal For Veterinary Medical Sciences 8 (1), 231-239 , 2017 2017.0 Citations: 3
Monooxygenase and dioxygenases by bacterial specific enzymes network JR Esmaeel, MAA Alfatlawi International Journal of Health Sciences, 10215-10222 , 2022 2022.0 Citations: 2
Molecular detection of invA, ssaP in Salmonella typhimurium isolated from chicken in Al-Qadisiyah Province JN Sadeq, JR Esmaeel, AA Neama AL-Qadisiyah Journal of Veterinary Medicine Sciences 16 (2), 8-13 , 2017 2017.0 Citations: 2
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Prevalence and biofilm formation of Staphylococcus spp. from some of diary products. JR Esmaeel, KH Fahad, BMM Al-Muhana Veterinary Practitioner 23 (2) , 2022 2022.0
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Phylogenetic Tree Analysis of Klebsiella pneumoniae Isolated from Bat Myotis emarginatus based on 16srRNA Prokaryote Sequence Alignment A Neamah, KH Fahad, J Esmaeel, J Sadeq Annals of the Romanian Society for Cell Biology 25 (1), 4664-4671 , 2021 2021.0
Direct detection of Shiga toxin producing by Escherichia coli by real-time PCR JR Esmaeel AL-Qadisiya Journal of Vet. Med. Sci. 14 (2), 78-82 , 2015 2015.0
Isolation EnterohemorrhagicEscherichia coli from gall bladder of buffaloes and determine its sensitivity for antibiotics B Mizher Kufa Journal For Veterinary Medical Sciences , 2014 2014.0
دراسة بكتريولوجية لقياس درجة التلوث الجرثومي وتأثيرها على نسبة الفقس جميلة راضي علاء عبد العزيز مجلة الكوفة للعلوم الطبية البيطرية 1 (العدد الأول), 18-27 , 2010 2010.0
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Bacteriological Stydy of pseudomonas aeruginosa Isolate from some pathogenic cases at diwaniya teaching hospital and hospital for obstetrics and pediatrics and its sensitivity … جميلة رضي اسماعيل, سجى مهدي ,بلسم ميري المحنة المجلة الطبية البيطرية العراقية 33 (2), 32-39 , 2009 2009.0
Effect of enzymes and metabolic pathway of genus Clostridium in forming simple molecules of different compound: A review JR Esmaeel, MAA Alfatlawi International Journal of Health Sciences, 10223-10231 , 0
