Firas Khaleyla
@unesa.ac.id
Faculty of Mathematics and Natural Sciences
Universitas Negeri Surabaya
EDUCATION
Master of Science in Biology
RESEARCH INTERESTS
Animal Physiology
Cell Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Muhammad Jakfar, Utama Alan Deta, Nurita Apridiana Lestari, Firas Khaleyla, Ratih Dewi Saputri, and Achmad Arrosyidi
EDP Sciences
STEM (Science, Technology, Engineering, and Mathematics) can help achieve the Sustainable Development Goals (SDGs) by fostering the development of 21st-century skills. It sparked a significant advancement in research on science education within the STEM field. This research intends to explore trends in STEM education for the SDGs by conducting a bibliometric analysis using the extensive Scopus databases. A total of 58 papers were reviewed from Scopus databases. A descriptive study method was employed to analyze the data. This study found that the number of STEM education and SDGs programs has increased over the past decade (2013-2023). The most prolific authors according to Scopus are seven authors. Over the past decade, Sustainability Switzerland has been the most significant source of documents on STEM education and SDGs. The VosViewer showcases six clusters associated with STEM Education and SDGs: Sustainable development goal, engineering education, science technology, quality education, stem education, and physics.
Erlix Rakhmad Purnama, Nur Kuswanti, Nur Qomariyah, Firas Khaleyla, and R. Ahmad Zainul Aziz
EDP Sciences
Renal complications are a major concern in individuals with diabetes, necessitating the search for effective antidiabetic agents with renoprotective properties. Phyllanthus acidus (L.) Skeels, commonly known as Cermai leaves, have been recognized for their medicinal properties, including potential antidiabetic effects. This in silico study aimed to investigate the renoprotective effects of Phyllanthus acidus as an antidiabetic agent by targeting the insulin-like growth factor-1 (IGF-1) (PDB code 1GZR) and angiotensin-converting enzyme 2 (ACE2) (PDB code 6M0J) receptors. The prediction of phytochemical properties was made based on the five laws of Lipinski as seen from the values of absorption, distribution, and metabolism with Swiss ADME. The in-silico method was conducted with the assistance of computer devices, databases like Protein Data Bank (PDB) and PubChem, and software like PyRx and Discovery Studio. The results showed the compounds kaempferol and cafestol meet the five laws of Lipinski. The compound kaempferol has the lowest binding affinity, with a value of -5.8 kcal/mol on IGF-1 and -6.1 kcal/mol on ACE2 and cafestol, -5.6 kcal/mol on IGF-1 and -5.8 kcal/mol on ACE2. It’s compared to the native ligand value -3.9 kcal/mol on IGF-1 and -5.2 kcal/mol on ACE2. Based on the results, it is known that this compound has a high potential to be a drug candidate for renoprotective.
Nithasya Nabilla, Imarotus Shofiyah, Sugiharto, Dita Alvitasari, Sri Sumarsih, Firas Khaleyla, Ismiliana Wirawati, and Dwi Winarni
Elsevier BV
Alan Verangga, Nur Qomariyah, and Firas Khaleyla
Bogor Agricultural University
Averrhoa bilimbi leaf extract (BLE) contains high antioxidant levels. Antioxidants can suppress reactive oxygen species produced during hyperglycemic conditions in diabetes mellitus (DM), which cause damage to liver tissue. This study aimed to determine the BLE effect on fasting blood glucose (FBG), Hepatosomatic Index (HSI), and liver tissue damage. We used 24 mice in 6 different groups, divided by N (normal), K- (DM), K+ (DM+glibenclamide 0.013mg/20gBW), E1 (DM+ extract 6.3mg/20gBW), E2 (DM+ extract 8.4mg/20gBW), and E3 (DM + extract 10.5mg/20gBW). We used an alloxan dose of 120mg/kgBW to induce the DM and then treated with BLE for 14 days. We measured the fasting blood glucose using a glucometer. In addition, we evaluated the liver tissue damage with HSI and Hematoxylin-Eosin (HE) stained histological slides. The results showed that BLE significantly reduced the percentage of FBG and liver tissue damage, while HSI showed no significant difference. The most optimal extract dose was 8.4 mg/20gBW (E2 group), with an FBG decrease of 26.44%, a normal cell percentage of 88.56%, and an HSI score of 6.18%. Based on this finding, we concluded that bilimbi leaf extract could lower blood glucose and improve liver histology of diabetic mice but did not significantly affect HSI.
Suhariningsih Suhariningsih, S. Glory, F. Khaleyla, H. N. Kusumawati, M. Septriana, Y. Susilo, A. K. Yaqubi, S. D. Astuti, and A. Syahrom
Hindawi Limited
Diabetes mellitus (DM) is a chronic metabolic disease or disorder characterized by high blood sugar levels as well as impaired carbohydrate, lipid, and protein metabolism due to insulin function insufficiency. Insulin deficiency can be caused by impaired or deficient insulin production by Langerhans beta cells in the pancreas or by a lack of responsiveness of the body's cells to insulin. This study aims to the effects of electrostimulation on the ameliorative (improves disease manifestations) or renoprotective (protects the kidneys) in a diabetic rat model using noninvasive (electrical stimulation with the magnetic and nonmagnetic electrode) and invasive (using needles) methods. This study used 25 female rats, with a normal control group (KN), a diabetes control group (KD), a needle treatment group (A), an electro-stimulator treatment group with a magnetic electrode (M), and an ES group with a nonmagnetic electrode (ES) (L). The electro-stimulator used AES-05 with a magnetic field strength of 90 mT at two acupoints, Pishu (BL20) and Shenshu (BL23). The treatment was administered 12 times in one month with a therapy time of 6.6 minutes per session. Body weight and blood sugar levels were compared before and after the treatment. After treatment, the diameter of the islets of Langerhans, as well as levels of creatinine and blood urea nitrogen (BUN), was measured. Furthermore, statistical analysis was performed (α = 0.05). The results of this study showed that electrical stimulation treatments with needle-invasive, noninvasive magnetic electrodes, and nonmagnetic electrodes significantly reduced diabetic rats’ blood glucose levels before and after the treatment. The analysis of the diameter of the islets of Langerhans revealed a significant difference between the treatment groups. The analysis of creatinine levels revealed a significant difference between groups, but creatinine levels in the group with the magnetic electrode (0.58 ± 0.17 mg/dL) were not significantly different from the control group (0.58 ± 0.07 mg/dL). The BUN test results revealed a significant difference compared with the diabetic control group, but no significant difference with the magnetic electrode treatment group. Conclusion. Based on the results, the most effective therapy for diabetes is a noninvasive method with magnetic (M) electrodes.
Reni Ambarwati, Dwi Anggorowati Rahayu, Fida Rachmadiarti, and Firas Khaleyla
UNS Solo
Abstract. Ambarwati R, Rahayu DA, Rachmadiarti F, Khaleyla F. 2021. DNA barcoding of lamp shells (Brachiopoda: Lingula anatina) from Probolinggo, East Java, Indonesia. Biodiversitas 22: 1764-1774. Lingula anatina is one of brachiopods found in tropical regions, however, the reports on this species from South East Asia is currently limited. This study, therefore, aims to identify the Cytochrome Oxidase subunit I (COI) sequence and phylogenetic of lamp shells from Probolinggo, Indonesia. A total of five samples of lamp shells were collected from Probolinggo, Indonesia, then identified based on morphological characters, and the identification was confirmed using molecular data. Subsequently, molecular characterization and identification were conducted based on 657 bp of COI gene similarity, sequence variation, genetic distance, phylogenetic topology, and BOLD System. According to the results, the Lingula species found in Probolinggo Indonesia was Lingula anatina. The median-joining network and Automatic Barcode Gap Discovery (ABGD) analysis showed this Lingula anatina population from Probolinggo had relatives of 22 haplotypes and divided into six haplogroups. Furthermore, the population belonged to the same haplogroup but had different haplotypes. Thus, this study provides more information for Lingula anatina phylogenetic and haplotype studies.
R Ambarwati, D A Rahayu, F Khaleyla, Wisanti, and E K Putri
IOP Publishing
Abstract A new coronavirus, SARS-CoV-2, is responsible for the current pandemic causing severe respiratory disease. It has been known that the receptor for SARS-CoV-2 entry into the host cell is the angiotensin-converting enzyme 2 (ACE2). This receptor is expressed in a wide array of organs, for example, the kidneys and gastrointestinal tract, but rarely present in the circulation. The soluble form of ACE2 proposed as a potential therapy for SARS-CoV-2 infection. This research aimed to explore angiotensin-converting enzyme 2 (ACE2) from marine biota as the source of ACE2, which is potential for the therapy of SARS-CoV-2 infection. This explorative study was conducted by retrieving the angiotensin-converting enzyme 2 (ACE2) from the database of protein (UniProt). The samples of the study were ACE2 of marine vertebrate, namely Delphinapterus leucas and ACE2 of marine invertebrate, namely Protunus trituberculatus. 3-D structures of ACE2 proteins unavailable in the protein database were modeled in Swiss Model. Molecular docking was conducted by using ClusPro.2.2. The data were analyzed descriptively. The molecular docking results revealed that the binding energy of spike glycoprotein of SARS-CoV-2 and human ACE2 was -988.5 kcal/mol. The binding energy of spike glycoprotein of SARS-CoV-2 and Delphinapterus leucas (Beluga whale) ACE2 was -946.4 kcal/mol. Meanwhile, the binding energy of spike glycoprotein of SARS-CoV-2 and Protunus trituberculatus (swimming crabs) ACE2 was -778.4 kcal/mol. The binding energy of spike glycoprotein of SARS-CoV-2 and Delphinapterus leucas (Beluga whale) ACE2 was closed to the binding energy of spike glycoprotein of SARS-CoV-2 and human ACE2. Hence, ACE2 of Delphinapterus leucas has the potential to be used as a therapeutic candidate from marine biota to suppress the SARS-CoV-2 transmission.
Wisanti, R Ambawati, EK Putri, DA Rahayu, and F Khaleyla
IOP Publishing
Abstract Students, parents, and teachers in Indonesia get the tremendous effects of the coronavirus (Covid-19 Pandemic) when schools are closed and the rules of Large-Scale Social Restrictions (LSSR) were set to overcome the global pandemic. While the government is doing its best to handle the outbreak of the epidemic, teachers respond and strive to provide qualified education for their students during these difficult times. Sciences should be learned through minds-on and hands-on, hence teachers must be able to create virtual classroom conditions that help students maintain learning momentum while they cannot interact each other physically. This study aimed to identify the difficulties experienced by science teachers during online learning and describe the efforts of science teachers to conduct online learning during the Covid-19 pandemic. The research data were obtained through a survey using a semi-structured online questionnaire. The respondents were 82 junior high school teachers and 104 biology high school teachers in Indonesia. Data analysis used a quantitative descriptive approach. The results showed that the majority of science teachers (77.5%) got difficulty in managing online learning. This difficulty was identified in three main factors namely technology, students, and teachers. Internet access was a technological factor that causes the greatest difficulty (42.4%) in online learning. Other difficulties came from the students by 21.5%, including low motivation, time management skills that were not optimal, and the lack of communication devices such as smartphones. The third factor, namely teachers, contributed 36.1% of difficulties, especially related to the explanation of concepts and the use of online learning applications. Based on these difficulties, 77.4% of teachers made an effort to carry out online learning optimally by presenting the material and providing slide presentation, discussion, as well as learning evaluation. A small percentage of teachers (22.6%) used online classes only for task assignments and learning evaluation. The efforts of science teachers to manage online learning have not optimized students’ varied learning experiences because science can’t be learned by reading and discussion only, but also by hands-on activities.
H. Fitrihidajati, F. Rachmadiarti, F. Khaleyla, and E. Kustiyaningsih
Technoscience Publications
The extensive use of detergent causes a high level of it to contaminate water body. This study was aimed to determine the effectiveness of the Sagittaria lancifolia plant as a phytoremediator for water contaminated with detergents. Sagittaria lancifolia was planted in medium exposed to detergent at various levels (10, 50, 75 ppm) with two different detention times (7 and 14 days). Remediated water was tested of its toxicity using Xiphophorus maculatus. LAS removal rate, BOD, plant morphology, and mortality of X. maculatus were recorded. The result showed that S. lancifolia had a high level of LAS removal rate, the longer detention time (81.53 ± 0.37% at 14 days) with significantly lower BOD (27.48 ± 0.78mg/L). Plant leaves showed signs of necrosis and chlorosis during detergent exposure. Detergent water remediated for 14 days induced the lowest rate of mortality in Xiphophorus maculatus. Thus, Sagittaria lancifolia can be applied to remove the organic contaminant from the water body.
Suhariningsih, Dwi Winarni, Saikhu A. Husen, Firas Khaleyla, Alfian Pramudita Putra, and Suryani D. Astuti
Springer Science and Business Media LLC
Saikhu Akhmad Husen, Septian Hary Kalqutny, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Firas Khaleyla, and Dwi Winarni
IOP Publishing
Abstract This study aimed to explore the effect of mangosteen pericarp extract used for reduce random blood glucose, total cholesterol, SGOT, SGPT, plasma creatinine levels, also ameliorates damaged liver hepatocytes and renal proximal tubular cells in diabetic mice. In this study, we used male mice (Mus musculus) of the BALB/C strain which were divided into 2 groups: the control group (without treatment of mangosteen pericarp extract) and the treatment group. The control group was divided into three: normal control (KN), diabetic control (KD), diabetic control-Metformin HCl (KM). The treatment group (with mangosteen pericarp extract) was divided into 3 groups (P1, P2, and P3) with the dose of 50 mg/kg body weight, 100 mg/kg body weight, and 200 mg/kg body weight, respectively. The induction of diabetes was done with the injection of multiple low-doses of STZ (30 mg/kg of body weight) for 5 consecutive days. Before and after STZ injection, random blood glucose and total cholesterol were measured at 1st, 7th, and 14th day of mangosteen pericarp extract treatments. Treatments were given for 14 days. At 15th day, SGOT, SGPT and plasma creatinine levels were measured using Pentra C200, while liver and kidney were collected and then processed into histological slides. Interestingly, we found that mangosteen pericarp extract administration was able to reduce random blood glucose, total cholesterol, SGOT, SGPT, plasma creatinine levels, also ameliorates damaged liver hepatocytes and renal proximal tubular cells in diabetic mice significantly. In conclusion, mangosteen pericarp extract is a promising antidiabetic agent due to its anti-hyperglycemic and antioxidant properties.
W Darmanto, S P A Wahyuningsih, S A Husein, N S Aminah, A N Firdaus, E S Sajidah, M Izzatin, and F Khaleyla
IOP Publishing
These research was design to evaluate the effect of 2-methoxyethanol (2-ME) injection to the number of damaged hepatocyte, renal tubule, and follicle. This study was designed as Completely Randomized Design experimental study. Animal model used was 2-3 months old 20 female mice strain Balb/C mice weighed at 25-30 g. Mice were divided into 4 treatment groups; N (normal control), G1 (given 200 mg/kg BW 2-ME), G2 (given 250 mg/kg BW 2-ME), and G3 (given 300 mg/kg BW 2-ME). 2-ME was administered intraperitoneally daily for 5 consecutive days. Liver, kidney, and ovarium were removed at the end of treatment period then processed into histological slides. Damage on hepatocyte, nephron, and follicle was evaluated from tissue sections. Data collected was analyzed statistically. Result showed that level of damage increased along with 2-ME dose given to mice. KP3 was found to have highest number of damaged cell in the three organs, followed by KP2, and KP1 had the lowest number of damaged cells compared to KN. Injection of 2-ME at various doses could induce damage on hepatocyte, renal tubule, and ovarian follicle.
Saikhu Akhmad Husen, Dwi Winarni, Firas Khaleyla, Septian Hary Kalqutny, and Arif Nur Muhammad Ansori
Author(s)
This study aimed to explore the activity of pericarp extract of mangosteen (Garcinia mangostana L.). Mangosteen pericarp contains various active compounds which are beneficial for human health. In-vivo antioxidant assay of pericarp extract was carried out using 3-4 month male mice of strain BALB/c weighed 30-40 g. The mice were divided into two groups: normal control (KN) group and STZ-induced diabetic group. STZ induction was performed using multiple low-dose method 30 mg/kg body weight treated daily for five consecutive days. Diabetic group was separated into two subgroups: diabetic control (KD), metformin control (KM), and crude extract treatment subgroups. The fasting blood glucose and the cholesterol level were measured before and after lard treatment, we also did it on the first, seventh, and fourteenth day of mangosteen pericarp crude extract treatment. The mice were treated with mangosteen pericarp crude extract for 14 days. The MDA level of the fasting blood serum was measured. The body weight an...