Investigating the impact of phthalate exposure on endocrine function in women with polycystic ovary syndrome Jalpa Patel, Hiral Chaudhary, Sonal Panchal, Kiransinh Rajput, Rushikesh Joshi BMC Endocrine Disorders, 2026 BACKGROUND: Polycystic Ovary Syndrome (PCOS) is a complex endocrine disorder influenced by genetic, hormonal, and environmental factors. In the environmental context, phthalate esters, specifically Mono-2-ethylhexyl phthalate (MEHP) and Di-2-ethylhexyl phthalate (DEHP), have emerged as potential endocrine disruptors, demanding a mysterious role in PCOS. OBJECTIVE: The study aimed to compare demographic, biochemical parameters, and levels of phthalate esters, specifically MEHP and DEHP, in women with and without PCOS. The association between these phthalate esters and different biochemical markers was also of interest for investigation. METHODS: The study included 160 participants, 90 in the PCOS group and 70 in the control group. Demographic and biochemical parameters were measured and compared for both groups. The levels of MEHP and DEHP in serum were determined using High-Performance Liquid Chromatography (HPLC). RESULTS: The PCOS group had a significantly higher average age and Body Mass Index (BMI) compared to the control group (p < 0.0001). Testosterone and luteinizing hormone (LH) levels were significantly elevated in the PCOS group (p < 0.05). In contrast, estradiol, follicle-stimulating hormone (FSH), prolactin, LH/FSH ratio, dehydroepiandrosterone sulfate, and thyroid-stimulating hormone (TSH) levels showed no significant difference. Notably, MEHP and DEHP levels were significantly higher in the PCOS group compared to the control group (47.33 ± 27.67 vs. 31.09 ± 18.74, p < 0.0001 and 31.03 ± 25.76 vs. 22.98 ± 19.65, p = 0.03 respectively). DEHP levels in the PCOS group demonstrated significant positive correlations with LH levels, LH/FSH ratio, and estradiol levels. In contrast, MEHP levels showed no significant correlations with the evaluated biochemical parameters. Neither MEHP nor DEHP displayed significant correlations with any examined parameters in the control group. CONCLUSION: This study underscores the elevated levels of MEHP and DEHP in women with PCOS, highlighting the potential influence of these environmental toxins in PCOS pathogenesis. CLINICAL TRIAL NUMBER: Not applicable.
Fungal allies in textile wastewater treatment: Reactive dye decolorization Hiral S. Surti, Vikram H. Raval, Rakeshkumar R. Panchal, Kiransinh N. Rajput Research Journal of Biotechnology, 2026 The main area of interest in study is the decolorization of such hazardous materials such as dyes. The biodegradation of dyes offers a cost-effective and sustainable alternative. This study investigates the decolorization and degradation potential of various reactive dyes by Aspergillus niger HSS16, emphasizing the optimization of key process parameters. The experimental findings reveal distinct decolorization efficiencies, with Reactive Blue 160 exhibiting the highest removal (91%) and with Reactive Orange P2R as the lowest (87%). Systematic analyses of pH, temperature, spore count, dye concentration, carbon source and nitrogen source influence on decolorization efficiency provide valuable insights. Results indicate that A. niger HSS16 achieved optimal decolorization at pH 4 and 5 for certain dyes, with temperature and dye concentration affecting decolorization efficiency inversely. The study further explores the impact of spore count, carbon source (favoring glucose) and nitrogen source (peptone yielding highest decolorization) on dye degradation. HPLC analysis substantiates the biodegradation process, highlighting the disappearance of primary peaks and the appearance of additional minor peaks in decolorized dye products. Future prospects include process optimization, scaleup for industrial application and molecular investigations for a comprehensive understanding of A. niger HSS16's dye-degrading capabilities, offering potential solutions for sustainable textile wastewater treatment.
Comprehensive evaluation of the multifaceted bioactivities of exopolysaccharides derived from marine bacteria Halomonas sp. DU-15-9-1 and Salinicoccus roseus S-5-9-3 Dhritiksha Mansukhlal Baria, Nidhi Yogeshbhai Patel, Shivani Vikram Raval, Rakeshkumar Ramanlal Panchal, Kiransinh N. Rajput, Vikram Hiren Raval Indian Journal of Biochemistry and Biophysics, 2026 The increasing demand for biodegradable, sustainable, and multifunctional biopolymers has intensified research on microbial exopolysaccharides (EPSs), particularly from extremophilic microorganisms. However, the bioactive potential of EPSs derived from halophilic bacteria remains underexplored. Therefore, the present study aimed to investigate. Exopolysaccharides (EPSs) are high molecular weight biopolymers composed predominantly of sugar monomers, exhibiting diverse chemical structures and properties. The structural diversity of EPSs from halophilic bacteria underpins their utility in environmental remediation and cosmetic formulations, owing to their physicochemical stability and functional properties. In a comprehensive study, two bacterial isolates, Halomonas sp. DU-15-9-1 and Salinicoccus roseus S-5-9-3 were examined for their capacity to synthesize EPSs with bioactive potential. The extraction and purification of EPSs from these isolates revealed significant functional activities. The EPS derived from both Halomonas sp. DU-15-9-1 (52.45%) and S. roseus S-5-9-3 (55.45%) demonstrated an emulsifying activity with coconut oil and groundnut oil, respectively, suggesting their potential as an emulsifying agent. Additionally, the antioxidant assays exhibited 52.68% and 64.82% radical scavenging activity, suggesting their usage as natural antioxidants. While the flocculating activity, a noteworthy feature for particulate aggregation in environmental processes, was 93% and 92%, respectively. The IC50 values against HeLa cells of 104.10 ± 9.26 µg/mL and 96.05 ± 5.54 µg/mL and against MCF-7 breast cancer cells 31.09 ± 0.63 µg/mL and 24.19 ± 0.22 µg/mL EPSs, respectively, demonstrate their cytotoxic effect and potential use as an anticancer agent. Thus, the multifaceted applications of microbial EPSs will certainly pave the way for futuristic research and biotechnological applications in the areas of food, cosmetics, environmental management, and pharmaceuticals.
Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83 Dimple S. Pardhi, Khushbu Rabadiya, Rushikesh G. Joshi, Vikram H. Raval, Rakeshkumar R. Panchal, Kiransinh N. Rajput Microbe Netherlands, 2025 Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is the member of starch degrading enzymes that catalyses an intramolecular transglycosylation reaction to produce cyclodextrins. CGTase producing 141 isolates were obtained from different agricultural fields (paddy, corn, potato, sorghum, and millet), gardens, and industrial waste soil samples. Amongst these 141, an alkaliphilic CGTase producing bacterial isolate Paenibacillus daejeonensis P-83 was selected. Although the CGTase showed the ability to produce α-, β-, and γ-cyclodextrins, the optimization studies were focused on β-CD production. One-factor-at-a-time (OFAT) increased the initial CGTase production from 1.05 U/ml to 2.76 U/ml with optimal conditions like, 3 % (w/v) water chestnut flour, 0.5 % (w/v) peptone, 0.5 % (w/v) yeast extract, 0.02 % (w/v) MgSO 4 , 0.1 % (w/v) K 2 HPO 4 , pH 11 ± 0.2, 6 % (v/v) inoculum, and incubation at 30 °C ± 0.2 for 72 hours on a rotary shaker (150 rpm). The CGTase production was increased upto 5.23 U/ml by statistical optimization via Central Component Design (CCD) using three variables i.e., water chestnut flour (3.65 %; w/v), peptone (1.1 %; w/v), and yeast extract (0.5 %; w/v). The optimization process significantly increased the CGTase production by 2.55 and 4.9 times using OFAT and CCD, respectively. • A potential CGTase producing bacterial isolate Paenibadcillus daejeonensis P-83 was isolated from millet farm soil sample. • This P. daejeonensis P-83 CGTase was able to produce three types of cyclodextrins (α, β, and γ). • The optimization of CGTase production using OFAT and CCD resulted into about five times increased enzyme. • A raw starch substrate, water chestnut flour was found as the best low-cost carbon source for higher CGTase production.
Microbial biosurfactants and bioemulsifiers: Properties, types and applications in cosmetic industry Dhritiksha M. Baria, Nidhi Y. Patel, Shivani Yagnik Raval, Rakeshkumar R. Panchal, Kiransinh N. Rajput, Vikram H. Raval Microbial Surfactants in Pharmaceuticals and Cosmetics, 2025 Microbial surfactants comprise biosurfactants and bioemulsifiers which are natural products with great potential in cosmetic formulation. Cosmetics are an essential part of current era. Number of people use a wide range of cosmetic items, including soap, shampoo, toothpaste, deodorant, skin care, perfume, and makeup. The cosmetics industry has a number of negative environmental, social, and economic effects that are being addressed by looking for more effective manufacturing processes, reducing waste and emissions, and promoting personal hygiene. These efforts improve public health while also creating job opportunities. The search for natural ingredients in cosmetic goods is currently popular among customers because many of these products offer comparable, superior, or additional benefits to those offered by chemical-based products. Compared to chemically produced surfactants, biosurfactants have various advantages, including improved biodegradability, better environmental compatibility, higher foaming properties, and preserved activity even at high temperatures and pH levels. Many synthetic surfactants and emulsifiers have significant levels of toxicity and ecological effect, which has sparked interest in more natural compounds like biosurfactants and bioemulsifiers. These molecules have an encouragingly low toxicology profile, making them suitable for use in the cosmetic, food, pharmaceutical, and environmental bioremediation.
Microbial enzymes in pharmaceutical industry Nidhi Y. Patel, Dhritiksha M. Baria, Dimple S. Pardhi, Shivani M. Yagnik, Rakeshkumar R. Panchal, Kiransinh N. Rajput, Vikram H. Raval Biotechnology of Microbial Enzymes Production Biocatalysis and Industrial Applications Second Edition, 2023
ACC Deaminase Producing Plant Growth Promoting Bacteria: Mechanisms, Stress Mitigation and Prospects for Sustainable Agriculture D Patel, R Joshi, V Raval, R Panchal, K Rajput Current Microbiology 83 (7), 361 , 2026 2026
Statistical optimization of cow products for an organic liquid panchagavya and its effect on Arachis hypogaea R Gohil, D Patel, K Rajput Vegetos, 1-9 , 2026 2026
Development of a liquid bioformulation using salt-tolerant plant growth-promoting Bacillus spp. from fermented panchagavya for salt stress mitigation D Patel, R Joshi, V Raval, R Panchal, K Rajput Systems Microbiology and Biomanufacturing 6 (3), 92 , 2026 2026
Statistical optimization and metabolite analysis of Acid Yellow 137 dye biodegradation by bacterial consortium RM Kathrotiya, B Raval, SV Raval, RR Panchal, KN Rajput, VH Raval Scientific Reports , 2026 2026
Comprehensive evaluation of the multifaceted bioactivities of exopolysaccharides derived from marine bacteria Halomonas sp. DU-15-9-1 and Salinicoccus roseus S-5-9-3 DM Baria, NY Patel, SV Raval, RR Panchal, KN Rajput, VH Raval Indian Journal of Biochemistry and Biophysics (IJBB) 63 (5), 546-556 , 2026 2026
Investigating the impact of phthalate exposure on endocrine function in women with polycystic ovary syndrome J Patel, H Chaudhary, S Panchal, K Rajput, R Joshi BMC Endocrine Disorders , 2026 2026 Citations: 2
Design, synthesis, antimicrobial and antioxidant evaluation, cytotoxicity assessment, and molecular dynamics-based computational studies of novel PABA analogues as dual DHPS … B Devliya, AK Al-Kubeisi, D Patel, M Dabhi, B Patel, M Thakar, ... RSC advances 16 (28), 26099-26126 , 2026 2026
Sustainable Biopolymers: Economic Feasibility of Microbial Exopolysaccharides Production DM Baria, SM Yagnik, RR Panchal, KN Rajput, VH Raval Extracellular Polysaccharides, 423-442 , 2025 2025
The evolution in antifungal treatments: from chemical drugs to biological agents AFH Faisal, RR Panchal, NJ Dawood, D Goswami, K Rajput, M Saraf Wisdom Journal For Studies & Research 5 (03), 210-231 , 2025 2025
Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83 DS Pardhi, K Rabadiya, RG Joshi, VH Raval, RR Panchal, KN Rajput The Microbe 7, 100358 , 2025 2025 Citations: 2
Mechanistic Insights of Plant-Microbe Interactions in Mitigation of Salinity Stress D Patel, D Pardhi, T Rathod, A Solanki, VH Raval, RR Panchal, R Joshi, ... Climate Change and Soil Microorganisms for Environmental Sustainability, 265-292 , 2025 2025 Citations: 3
Soil Actinomycetes with a Perspective of Cellulolytic Activity A Patani, J Pandya, A Vaghela, J Thakor, H Vasava, KN Rajput, VH Raval, ... Climate Change and Soil Microorganisms for Environmental Sustainability, 47-63 , 2025 2025
ISOLATION AND CHARACTERIZATION OF CELLULASE–PRODUCING ACTINOMYCETES FROM AGRICULTURAL SOILS OF GUJARAT, INDIA: POTENTIAL FOR LIQUID LIGNOCELLULOSIC WASTE DEGRADATION A Patani, J Pandya, A Vaghela, J Thakor, V Raval, K Rajput, R Panchal VIDYA-A JOURNAL OF GUJARAT UNIVERSITY 4 (1), 148-159 , 2025 2025
Unveiling the potential of Bacillus safensis PG-54 isolated from fermented panchagavya on Sorghum bicolor under salinity stress D Patel, D Pardhi, T Rathod, A Solanki, V Raval, R Panchal, R Joshi, ... Discover Plants 2 (1), 87 , 2025 2025 Citations: 6
Exploring the potential of Shouchella oshimensis P-106 for cyclodextrin glucanotransferase production D Pardhi, K Rabadiya, R Joshi, V Raval, R Panchal, K Rajput VIDYA-A JOURNAL OF GUJARAT UNIVERSITY 4 (1), 1-9 , 2025 2025 Citations: 1
Microbial Biosurfactants and Bioemulsifiers: Properties, Types and Applications in Cosmetic Industry DM Baria, NY Patel, SY Raval, RR Panchal, KN Rajput, VH Raval Microbial Surfactants in Pharmaceuticals and Cosmetics, 144-172 , 2025 2025 Citations: 2
STATISTICAL OPTIMIZATION OF ALKALINE-PROTEASE PRODUCTION FROM NESTERENKONIA SP. K-15-9-6 ISOLATED FROM THE GULF OF KHAMBHAT, GUJARAT, INDIA PS ARYA, SHV RAVAL, KN RAJPUT, RR PANCHAL, VH RAVAL RESEARCH JOURNAL OF BIOTECHNOLOGY 20 (3), 64-74 , 2025 2025
Purification and characterization of cyclodextrin glucanotransferase by Paenibacillus daejeonensis P-83 DS Pardhi, K Rabadiya, RG Joshi, RR Panchal, VH Raval, KN Rajput Next Bioengineering 1, 100002 , 2025 2025
A review on recent upgradation and strategies to enhance cyclodextrin glucanotransferase properties for its applications K Rabadiya, D Pardhi, K Thaker, J Patoliya, K Rajput, R Joshi International Journal of Biological Macromolecules 259, 129315 , 2024 2024 Citations: 16
Understanding the Fermentation Potentiality For Gibberellic Acid (GA 3 ) Production Using Fungi JB Pandya, AN Patani, VH Raval, KN Rajput, RR Panchal Current Microbiology 80 (12), 385 , 2023 2023 Citations: 12
MOST CITED SCHOLAR PUBLICATIONS
Plant chitinases and their role in plant defense: A comprehensive review B Vaghela, R Vashi, K Rajput, R Joshi Enzyme and Microbial Technology 159, 110055 , 2022 2022 Citations: 241
Microbial surfactants: a journey from fundamentals to recent advances DS Pardhi, RR Panchal, VH Raval, RG Joshi, P Poczai, WH Almalki, ... Frontiers in microbiology 13, 982603 , 2022 2022 Citations: 124
Valorization of agro-food wastes: Ease of concomitant-enzymes production with application in food and biofuel industries PS Arya, SM Yagnik, KN Rajput, RR Panchal, VH Raval Bioresource technology 361, 127738 , 2022 2022 Citations: 99
Plant Growth-Promoting Activity of Bacillus sp. PG-8 Isolated From Fermented Panchagavya and Its Effect on the Growth of Arachis hypogea RB Gohil, VH Raval, RR Panchal, KN Rajput Frontiers in Agronomy 4, 805454 , 2022 2022 Citations: 90
β ‐Cyclodextrin Production by Cyclodextrin Glucanotransferase from an Alkaliphile Microbacterium terrae KNR 9 Using Different Starch Substrates KN Rajput, KC Patel, UB Trivedi Biotechnology Research International 2016 (1), 2034359 , 2016 2016 Citations: 55
Assessment of gut microbial β-glucuronidase and β-glucosidase activity in women with polycystic ovary syndrome J Patel, H Chaudhary, K Rajput, B Parekh, R Joshi Scientific Reports 13 (1), 11967 , 2023 2023 Citations: 40
Understanding the basis of occurrence, biosynthesis, and implications of thermostable alkaline proteases PS Arya, SM Yagnik, KN Rajput, RR Panchal, VH Raval Applied biochemistry and biotechnology 193 (12), 4113-4150 , 2021 2021 Citations: 35
Plant growth promoting activities and effect of fermented panchagavya isolate Klebsiella sp. PG-64 on Vigna radiata RB Gohil, VH Raval, RR Panchal, KN Rajput World Journal of Microbiology and Biotechnology 39 (2), 41 , 2023 2023 Citations: 26
A novel cyclodextrin glucanotransferase from an alkaliphile Microbacterium terrae KNR 9: purification and properties KN Rajput, KC Patel, UB Trivedi 3 Biotech 6 (2), 168 , 2016 2016 Citations: 25
Screening and Selection of Medium Components for Cyclodextrin Glucanotransferase Production by New Alkaliphile Microbacterium terrae KNR 9 Using Plackett … KN Rajput, KC Patel, UB Trivedi Biotechnology Research International 2016 (1), 3584807 , 2016 2016 Citations: 23
Bio-prospecting the future in perspective of amidohydrolase L-glutaminase from marine habitats NY Patel, DM Baria, SM Yagnik, KN Rajput, RR Panchal, VH Raval Applied microbiology and biotechnology 105 (13), 5325-5340 , 2021 2021 Citations: 20
Statistical optimization of medium components for biosurfactant production by Pseudomonas guguanensis D30 DS Pardhi, RR Panchal, VH Raval, KN Rajput Preparative Biochemistry & Biotechnology 52 (2), 171-180 , 2022 2022 Citations: 19
Screening of Biosurfactant Producing Bacteria and Optimization of Production Conditions for Pseudomonas guguanensis D30 RPKR Dimple Pardhi Bioscience Biotechnology Research Communications 13 (Special Issue Vol 13 No … , 2020 2020 Citations: 19
A review on recent upgradation and strategies to enhance cyclodextrin glucanotransferase properties for its applications K Rabadiya, D Pardhi, K Thaker, J Patoliya, K Rajput, R Joshi International Journal of Biological Macromolecules 259, 129315 , 2024 2024 Citations: 16
Microbial enzymes in pharmaceutical industry NY Patel, DM Baria, DS Pardhi, SM Yagnik, RR Panchal, KN Rajput, ... Biotechnology of microbial enzymes, 375-403 , 2023 2023 Citations: 16
Exopolysaccharides from marine microbes with prowess for environment cleanup DM Baria, NY Patel, SM Yagnik, RR Panchal, KN Rajput, VH Raval Environmental Science and Pollution Research 29 (51), 76611-76625 , 2022 2022 Citations: 14
Understanding the Fermentation Potentiality For Gibberellic Acid (GA 3 ) Production Using Fungi JB Pandya, AN Patani, VH Raval, KN Rajput, RR Panchal Current Microbiology 80 (12), 385 , 2023 2023 Citations: 12
Isolation, production and applications of alkaline protease from hot springs bacterial isolates P Arya, M Trivedi, N Ramani, K Patel, KN Rajput Bioscience Biotechnology Research Communication , 2020 2020 Citations: 10
Isolation and Screening of Plant Growth Promoting Bacteria from Fermented Panchagavya MDKR Rinkal Gohil, Khushboo Patel, Hitarthi Shah, Saloni Vyas Bioscience Biotechnology Research Communications 13 (Special Issue Vol 13 No … , 2020 2020 Citations: 9