Isti Koesharyani

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Research Centre for Fisheries, Research Organization Earth Sciences and Maritime,
National Research and Innovation Agency

Isti Koesharyani


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RESEARCH INTERESTS

Fish and shrimp diseases: Diagnosis, Virology, Parasitology, Bacteriology, Cell culture and Vaccine development
13

Scopus Publications

Scopus Publications

  • Natural spawning and larval rearing of the longfin yellowtail (Seriola rivoliana Valenciennes, 1833) in captivity in Indonesia
    Aacl Bioflux, 2025
  • First Report of Scale Drop Disease in Hatchery-produced Asian Seabass (Lates calcarifer) in Indonesia
    International Journal of Veterinary Science, 2025
  • Effects of honey saccharide supplementation on growth performance, amylase enzyme activity, gut microvilli, and microbiome in Cyprinus carpio
    Yani Aryati, Ekorini Farastuti, Lili Sholichah, Isti Koesharyani, Lila Gardenia, Early Septiningsih, Muhamad Yamin, Parwa Oryzanti, Dewi Puspaningsih, Desy Sugiani
    Veterinary World, 2025
    Background and Aim: Prebiotics, such as saccharides in honey, play a crucial role in improving gut microbiota, digestion, and immune function. This study evaluates the effects of Kapok flower honey saccharides on growth performance, digestive enzyme activity, intestinal morphology, and gut microbiota in common carp (Cyprinus carpio). Materials and Methods: A completely randomized design was implemented with four honey supplementation levels (0% control, 0.5%, 0.75%, and 1%) applied to juvenile C. carpio diets over 30 days. Growth performance, feed utilization, intestinal microvilli structure, gut microbiota, and amylase activity were analyzed using advanced techniques, including high performance liquid chromatography, scanning electron microscopy, and biochemical assays. Results: Kapok flower honey contains fructooligosaccharides (FOS, 14.76%) and inulin (6.6%). Supplementation at 1% significantly improved weight gain, feed conversion ratio, and specific growth rate. Amylase activity increased with honey supplementation, peaking at 24.13 ± 3.11 U g⁻¹ protein for the 1% group. Gut morphology analysis revealed longer, denser intestinal microvilli and higher perimeter ratios in honey-treated groups than controls. Microbiota analysis showed increased beneficial Bacillus spp. exclusively in the honey-supplemented groups. Conclusion: Honey saccharides, particularly FOS and inulin, significantly enhance the growth performance, digestive enzyme activity, and gut health of common carp. Supplementation with 1% honey is optimal, improving feed efficiency and fostering beneficial gut microbiota. These findings highlight honey as a cost-effective, natural prebiotic for aquaculture. Keywords: amylase, gut microbiome, honey saccharides, microvilli, prebiotics.
  • A new species of pisang shrimp, Penaeus symplex revealed by molecular DNA analyses, as candidate species for aquaculture in Indonesia
    Ketut Sugama, Abidin Nur, Isti Koesharyani, Haryanti, Hans. P. Saluz
    Bio Web of Conferences, 2024
    In Indonesia there are two species mainly farmed, these are Penaeus monodon and Litopenaeus vannamei. Due to disease outbreaks which were mainly caused by viruses, shrimp aquaculture is become a high risk, therefore is a need to find new species. Unknown species of shrimp named as udang pisang (Pisang shrimp) only found in Indonesian waters. In the present study, six species of shrimp available in Indonesia, those are Pisang shrimp (Penaeus sp.), P. monodon, P. merguensis, P. indicus, P. semisulcatus and L. vannamei were analysed by RAPD with two primers of 2AAM2 and AS15 to confirm their species. Preliminary trials on the culture of Pisang shrimp and P. monodon were carried out in earthen ponds to compare their growth. The results revealed that based on the gel banding pattern and molecules weight of DNA, Pisang shrimp genetically is different from other five species analyzed and morphology generally like P. monodon. The identity between Pisang shrimp and P. monodon was 92%, suggested the two species are different. Pisang shrimp is a new species named Penaeus symplex [14, 20] and has been validated. The growth of Pisang shrimp and P. monodon for 90 days of culture was comparable (19.89f1.71 g and 20.0111.08 g). Therefore, Pisang shrimp has high potential for aquaculture diversification.
  • The potential of endogenous bacteria as probiotics to enhance survival, growth, and immunity of cultured spiny lobster Panulirus homarus (Linnaeus 1758)
    Aacl Bioflux, 2024
  • SIMULTANEOUS PATHOGEN DETECTION OF SHRIMP VIRUSES ON CULTURED TIGER SHRIMPS (Penaeus monodon) IN INDONESIA
    Isti Koesharyani, Putu Eka Sudaryatma, Lila Gardenia, Yani Aryati, Ketut Mahardika, Tatik Mufidah
    Indonesian Aquaculture Journal, 2023
    The multiple-pathogen infection causes severe economic impact to shrimp industry in Indonesia and worldwide due to mass mortality and multiple abnormalities of the survived infected shrimps. However, multiple-pathogen detection tools in shrimp diseases have not yet widely used. The purpose in this study was to develop and applied simultaneous detection system using multiplex polymerase chain reaction (PCR) assay from natural infections caused by white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV) and monodon baculovirus (MBV) in Black tiger shrimp culture. To analyze multiple-pathogen infections in the shrimp, the study designed and used three pairs of specific primers targeting DNA virus from the shrimp diseases. All amplifications used a specific master mix for multiplex PCR assay and standardized extracted nucleic acid from the samples. This mPCR assay successfully amplified the DNA of three viruses in a single tube-run by multiplex PCR for each virus. Based on the results, the study confirms that multiple-pathogen infection contributes the highest mass mortality rather than from single infection by either WSSV, IHHNV or MBV. This study also confirms that the mPCR assay is a faster, cheaper, and efficient method to detect and subsequently prevent the spreading of multi-pathogen shrimp diseases.
  • Study on milky haemolymph diseases infection in wild and cultured of spiny lobster, Panulirus homarus in Indonesia
    I Koesharyani, N L A Lasmika, K Sugama
    Iop Conference Series Earth and Environmental Science, 2021
    Milky Haemolymph Disease in Spiny Lobster (MHD-SL) is the most pathogenic diseases in spiny lobster (Panulirus homarus). Research on MHD-SL infection has not been undertaken in Indonesia. Therefore, present study aims to determine the infection of MHD-SL lobster. In 2016 a total of 240 lobsters for 30 each both from wild and cultured sample were collected from four locations (Candi Kusuma Bay of Bali Island, Gerupuk, Awang, and Telong-Elong Bays of Lombok Island) and in 2019, 50 lobster samples were collected for artificial infection study. While in January 2020, another 40 lobsters were collected from 2 different sites of culture (coastal and offshore cages) within Telong Elong Bay to determine infection of MHD-SL and for transmission study. The MHD-SL diseased was first check by clinical sign and confirmed by PCR-DNA molecular with specific primer of 254 bp. An experimental infection of MHD-SL was carried out by injection and cohabitation. The result showed that infected MHD-SL lobster shows inactive, loose appetite to eat, reddish and white colour of abdomen then moribund and all positive by PCR test. MHD-SL was found only in cultured lobster on the cages located at coastal water and no in the cages located at offshore within Telong-Elong Bay. In the experiment of artificial infection, either by injection or cohabitation, shows clinical sign of MHD-SL appeared at 8 days and all died after 14 days for both treatments. The present study approved that MHD-SL is pathogenic agent belonged to Rickettsia-like bacterium and infection occurred by horizontal transmission.
  • Fast short-fragment PCR for rapid and sensitive detection of shrimp viruses
    Grit Mrotzek, Haryanti, Isti Koesharyani, Alexander N. Tretyakov, Ketut Sugama, Hans P. Saluz
    Journal of Virological Methods, 2010
  • Effect of high water temperature on betanodavirus infection of fingerling humpback grouper Cromileptes altivelis
    Kei Yuasa, Isti Koesharyani, Ketut Mahardika
    Fish Pathology, 2007
    —Fingerling humpback grouper Cromileptes altivelis , experimentally infected with a betanodavirus (RGNNV genotype), were kept at 27 ∞ C, 31 ∞ C or 35 ∞ C for 14 days. The numbers of dead fish in ten fish at each water temperature were five at 27 ∞ C, two at 31 ∞ C and one at 35 ∞ C. The coat protein gene of the virus was detected by RT-PCR from four survivors at 27 ∞ C, two survivors at 31 ∞ C, but not from survivors at 35 ∞ C. Histopathologically, vacuolation in the retinal tissues was observed in three survivors at 27 ∞ C, but not in survivors at 31 ∞ C or 35 ∞ C. These results suggest that high water temperature inhibits the viral proliferation in fish.
  • Immune response of humpback grouper, Cromileptes altivelis (Valenciennes) injected with the recombinant coat protein of betanodavirus
    K Yuasa, I Koesharyani, D Roza, K Mori, M Katata, T Nakai
    Journal of Fish Diseases, 2002
    Journal of Fish DiseasesVolume 25, Issue 1 p. 53-56 Immune response of humpback grouper, Cromileptes altivelis (Valenciennes) injected with the recombinant coat protein of betanodavirus K Yuasa, K Yuasa Japan International Cooperation Agency, Yoyogi, Tokyo, Japan,Search for more papers by this authorI Koesharyani, I Koesharyani Gondol Research Institute for Mariculture, Singaraja, Bali, Indonesia,Search for more papers by this authorD Roza, D Roza Gondol Research Institute for Mariculture, Singaraja, Bali, Indonesia,Search for more papers by this authorK Mori, K Mori Kamiura Station, Japan Sea-Farming Association, Oita, Japan,Search for more papers by this authorM Katata, M Katata Fish Pathology Laboratory, Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, JapanSearch for more papers by this authorT Nakai, T Nakai Fish Pathology Laboratory, Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, JapanSearch for more papers by this author K Yuasa, K Yuasa Japan International Cooperation Agency, Yoyogi, Tokyo, Japan,Search for more papers by this authorI Koesharyani, I Koesharyani Gondol Research Institute for Mariculture, Singaraja, Bali, Indonesia,Search for more papers by this authorD Roza, D Roza Gondol Research Institute for Mariculture, Singaraja, Bali, Indonesia,Search for more papers by this authorK Mori, K Mori Kamiura Station, Japan Sea-Farming Association, Oita, Japan,Search for more papers by this authorM Katata, M Katata Fish Pathology Laboratory, Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, JapanSearch for more papers by this authorT Nakai, T Nakai Fish Pathology Laboratory, Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, JapanSearch for more papers by this author First published: 21 January 2002 https://doi.org/10.1046/j.1365-2761.2002.00325.xCitations: 48 Dr T Nakai Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima 739-8528, Japan (e-mail: nakaitt@hiroshima-u.ac.jp) Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume25, Issue1January 2002Pages 53-56 RelatedInformation
  • Viral nervous necrosis in humpback grouper Cromileptes altivelis larvae and juveniles in Indonesia
    Zafran, Isti Koesharyani, Fris Johnny, Kei Yuasa, Takahiko Harada, Kishio Hatai
    Fish Pathology, 2000
  • Two species of capsalid monogeneans infecting cultured humpback grouper Cromileptes altivelis in Indonesia
    Isti Koesharyani, Zafran, Kei Yuasa, Kishio Hatai
    Fish Pathology, 1999
  • Increased Survival of Penaeus monodon Larvae Treated with Vibrio harveyi Bacterin
    Zafran, Fris Johnny, Des Roza, Isti Koesharyani, Kishio Hatai
    Fish Pathology, 1997