Silvana Pinna

Scopus Publications

What it takes to solve the origins of life: An integrated review. Part 2: Theoretical methods and emerging trends
Silke Asche, Carla Bautista, Celia Blanco, David Boulesteix, Alexandre Champagne-Ruel, Cole Mathis, Omer Markovitch, Zhen Peng, Avinash Vicholous Dass, Alyssa Adams, Eloi Camprubi, Enrico Sandro Colizzi, Stephanie Colón-Santos, Hannah Dromiack, Valentina Erastova, Amanda Garcia, Ghjuvan Grimaud, Aaron Halpern, Stuart A. Harrison, Seán F. Jordan, Tony Z. Jia, Amit Kahana, Artemy Kolchinsky, Odin Moron-Garcia, Ryo Mizuuchi, Jingbo Nan, Yuliia Orlova, Ben K.D. Pearce, Klaus Paschek, Martina Preiner, Silvana Pinna, Eduardo Rodríguez-Román, Loraine Schwander, Siddhant Sharma, Harrison B. Smith, Andrey Vieira, Joana C. Xavier
Cell Reports Physical Science, 2026
The origin(s) of life (OoL), which has puzzled scientists for centuries, remains a major scientific challenge in the 21st century. Understanding the processes relevant to the OoL demands theoretical frameworks that can connect processes across scales, from microscopic dynamics to emergent levels of organization. While experimental studies generate a wealth of data, theoretical and computational approaches provide the structure necessary to interpret and generalize these findings. In Part 1, we examined the most widely used experimental techniques in the field. Here, we focus on the mathematical, physical, and computational techniques used to model phenomena relevant to life's origin(s). We discuss methods ranging from quantum chemistry and molecular dynamics to chemical reaction networks, autocatalysis, and evolutionary modeling, as well as information-theoretic and phylogenetic approaches that link chemical and biological organization. We further highlight emerging trends such as synthetic biology, omics-based methods, and laboratory automation as novel points of contact for theory-experiment integration. Ultimately, we aim to provide an educational tool that can facilitate more post-disciplinary collaborations in OoL research by helping scientists understand what they can do about the problem of life's origins, rather than telling them how to think about it.
What it takes to solve the origin of life: An integrated review. Part 1–Experimental methods and data repositories
Silke Asche, Carla Bautista, Celia Blanco, David Boulesteix, Alexandre Champagne-Ruel, Cole Mathis, Omer Markovitch, Zhen Peng, Avinash Vicholous Dass, Alyssa Adams, Eloi Camprubi, Enrico Sandro Colizzi, Stephanie Colón-Santos, Hannah Dromiack, Valentina Erastova, Amanda Garcia, Ghjuvan Grimaud, Aaron Halpern, Stuart A. Harrison, Seán F. Jordan, Tony Z. Jia, Amit Kahana, Artemy Kolchinsky, Odin Moron-Garcia, Ryo Mizuuchi, Jingbo Nan, Yuliia Orlova, Ben K.D. Pearce, Klaus Paschek, Martina Preiner, Silvana Pinna, Eduardo Rodríguez-Román, Loraine Schwander, Siddhant Sharma, Harrison B. Smith, Andrey Vieira, Joana C. Xavier
Cell Reports Physical Science, 2026
<h2>Summary</h2> The origin(s) of life (OoL), which has puzzled scientists for centuries, remains a major scientific challenge in the 21st century. Research on OoL spans many disciplines, including chemistry, physics, biology, planetary sciences, computer science, and mathematics. The sheer number of different scientific perspectives relevant to the problem has resulted in the coexistence of diverse tools, techniques, data, and software in OoL studies. This has made communication between the disciplines relevant to the OoL extremely difficult because the interpretation of data, analyses, or standards of evidence varies dramatically. Here, we hope to bridge this wide field of study by providing common ground via the consolidation of techniques rather than positing a unifying view on how life emerges. In part 1 of this review, we cover common experimental techniques that have been used significantly in OoL studies in recent years, while in part 2, we review theoretical, computational, and integrative methods. Here, we discuss the use of spectroscopy, spectrometry, chromatography, microscopy, and sequencing methods for characterizing diverse materials. We further discuss the role of data repositories in facilitating the analysis and dissemination of experimental data. This review provides a baseline expectation and understanding of the analytical aspects of origins' research. Ultimately, we aim to provide an educational tool that can facilitate more post-disciplinary collaborations in OoL research by helping scientists understand what they can do about the problem of life's origins, rather than telling them how to think about it.
Exploring the diversity within EANA and AbGradE as mirror of the European astrobiology community
Lena Noack, Ruth-Sophie Taubner, Steffi Pohl, Silvana Pinna, Séverine Robert, Frédéric Foucher, Jean-Pierre Paul de Vera
International Journal of Astrobiology, 2026
Since its foundation in 2001, EANA (European Astrobiology Network Association, http://www.eana-net.eu/ ) has organized annual meetings to foster and strengthen the astrobiology community within Europe. The growth of the European astrobiology community over the years is reflected not only in the growing participation at the annual EANA meeting but also in the foundation of the graduate network AbGradE (Astrobiology Graduates in Europe, https://abgrade.eu/ ) with many activities, meetings and workshops organized since 2014, including several joint events with EPEC (the EuroPlanet Early Career network), as well as the foundation of the European Astrobiology Institute (EAI) in 2019. The EANA Executive Council consists of national representatives from currently 21 European (and affiliated) countries with active astrobiology groups, networks or societies, as well as representatives of the early-career AbGradE network. The EANA network and especially the Executive Council therefore directly promote a broad and diverse representation of many career stages as well as nationalities including normally underrepresented countries. After more than two decades of a formal astrobiology network in Europe, it is time to explore and evaluate the actual obtained diversity within EANA and AbGradE with respect to nationality, gender and career status of our members based on our annual meeting participation, and to reflect on future measures to further improve the diversity and inclusiveness of our networks and events. We find that while our annual events are very diverse with respect to career stage, gender and research disciplines, a few aspects leave room for improvement, including especially a more balanced representation of different countries with astrobiology research within Europe and beyond. We discuss different equality, diversity and inclusivity measures that can be implemented for future EANA and AbGradE meetings in Europe to better represent the full astrobiology community within our networks.
Metal/ADP Complexes Promote Phosphorylation of Ribonucleotides
Emilie Werner, Silvana Pinna, Robert J. Mayer, Joseph Moran
Journal of the American Chemical Society, 2023
Under enzyme catalysis, adenosine triphosphate (ATP) transfers a phosphoryl group to canonical ribonucleotide diphosphates (NDPs) to form ribonucleotide triphosphates (NTPs), the direct biosynthetic precursors to RNA. However, it remains unclear whether the phosphorylation of NDPs could have occurred in water before enzymes existed and why an adenosine derivative, rather than another canonical NTP, typically performs this function. Here, we show that adenosine diphosphate (ADP) in the presence of Fe3+ or Al3+ promotes phosphoryl transfer from acetyl phosphate to all canonical NDPs to produce their corresponding NTP in water at room temperature and in the absence of enzymes. No other NDPs were found to promote phosphorylation, giving insight into why adenosine derivatives specifically became used for this purpose in biology. The metal-ADP complexes also promote phosphoryl transfer to ribonucleoside monophosphates (NMPs) to form a mixture of the corresponding NDPs and NTPs, albeit less efficiently. This work represents a rare example in which a single nucleotide carries out a function critical to biology without enzymes. ADP-metal complexes may have played an important role in nucleotide phosphorylation in prebiotic chemistry.
Reaching out to early-career astrobiologists: AbGradE's actions and perspectives
Philippe Nauny, A. Cassaro, N. Kopacz, L. Noack, Hayk Palabikyan, S. Pinna, Alex Price, Hector A. Stavrakakis, R. Taubner
International Journal of Astrobiology, 2022
Astrobiology Graduates in Europe (AbGradE, pronounced ab-grad-ee) is an association of early-career scientists working in fields relevant to astrobiological research. Conceptualized in 2013, it was initially designed as a mini-conference or workshop dedicated to early-career researchers, providing a friendly environment where early-career minds would be able to present their research without being intimidated by the possibility of facing a more traditional audience, composed mainly of senior scientists. Within the last couple of years, AbGradE became the first point of call for European, but also for an increasing number of non-European, early-career astrobiologists. This article aims to present how AbGradE has evolved over the years (in its structure and in its way of organizing events), how it has adapted with the COVID-19 pandemic, and what future developments are considered.
A prebiotic basis for ATP as the universal energy currency
Silvana Pinna, Cäcilia Kunz, Aaron Halpern, Stuart A. Harrison, Sean F. Jordan, John Ward, Finn Werner, Nick Lane
Plos Biology, 2022
ATP is universally conserved as the principal energy currency in cells, driving metabolism through phosphorylation and condensation reactions. Such deep conservation suggests that ATP arose at an early stage of biochemical evolution. Yet purine synthesis requires 6 phosphorylation steps linked to ATP hydrolysis. This autocatalytic requirement for ATP to synthesize ATP implies the need for an earlier prebiotic ATP equivalent, which could drive protometabolism before purine synthesis. Why this early phosphorylating agent was replaced, and specifically with ATP rather than other nucleoside triphosphates, remains a mystery. Here, we show that the deep conservation of ATP might reflect its prebiotic chemistry in relation to another universally conserved intermediate, acetyl phosphate (AcP), which bridges between thioester and phosphate metabolism by linking acetyl CoA to the substrate-level phosphorylation of ADP. We confirm earlier results showing that AcP can phosphorylate ADP to ATP at nearly 20% yield in water in the presence of Fe3+ ions. We then show that Fe3+ and AcP are surprisingly favoured. A wide range of prebiotically relevant ions and minerals failed to catalyse ADP phosphorylation. From a panel of prebiotic phosphorylating agents, only AcP, and to a lesser extent carbamoyl phosphate, showed any significant phosphorylating potential. Critically, AcP did not phosphorylate any other nucleoside diphosphate. We use these data, reaction kinetics, and molecular dynamic simulations to infer a possible mechanism. Our findings might suggest that the reason ATP is universally conserved across life is that its formation is chemically favoured in aqueous solution under mild prebiotic conditions.
Do Soluble Phosphates Direct the Formose Reaction towards Pentose Sugars?
E. Camprubi, S. Harrison, S. Jordan, J. Bonnel, S. Pinna, N. Lane
Astrobiology, 2022
The formose reaction has been a leading hypothesis for the prebiotic synthesis of sugars such as ribose for many decades but tends to produce complex mixtures of sugars and often tars. Channeling the formose reaction towards the synthesis of biologically useful sugars such as ribose has been a holy grail of origins-of-life research. Here, we tested the hypothesis that a simple, prebiotically plausible phosphorylating agent, acetyl phosphate, could direct the formose reaction towards ribose through phosphorylation of intermediates in a manner resembling gluconeogenesis and the pentose phosphate pathway. We did indeed find that addition of acetyl phosphate to a developing formose reaction stabilized pentoses, including ribose, such that after 5 h of reaction about 10-fold more ribose remained compared with control runs. But mechanistic analyses using liquid chromatography-mass spectrometry showed that, far from being directed towards ribose by phosphorylation, the formose reaction was halted by the precipitation of Ca2+ ions as phosphate minerals such as apatite and hydroxyapatite. Adding orthophosphate had the same effect. Phosphorylated sugars were only detected below the limit of quantification when adding acetyl phosphate. Nonetheless, our findings are not strictly negative. The sensitivity of the formose reaction to geochemically reasonable conditions, combined with the apparent stability of ribose under these conditions, serves as a valuable constraint on possible pathways of sugar synthesis at the origin of life.
Acetyl Phosphate as a Primordial Energy Currency at the Origin of Life
Alexandra Whicher, E. Camprubi, S. Pinna, B. Herschy, N. Lane
Origins of Life and Evolution of Biospheres, 2018
Metabolism is primed through the formation of thioesters via acetyl CoA and the phosphorylation of substrates by ATP. Prebiotic equivalents such as methyl thioacetate and acetyl phosphate have been proposed to catalyse analogous reactions at the origin of life, but their propensity to hydrolyse challenges this view. Here we show that acetyl phosphate (AcP) can be synthesised in water within minutes from thioacetate (but not methyl thioacetate) under ambient conditions. AcP is stable over hours, depending on temperature, pH and cation content, giving it an ideal poise between stability and reactivity. We show that AcP can phosphorylate nucleotide precursors such as ribose to ribose-5-phosphate and adenosine to adenosine monophosphate, at modest (~2%) yield in water, and at a range of pH. AcP can also phosphorylate ADP to ATP in water over several hours at 50 °C. But AcP did not promote polymerization of either glycine or AMP. The amino group of glycine was preferentially acetylated by AcP, especially at alkaline pH, hindering the formation of polypeptides. AMP formed small stacks of up to 7 monomers, but these did not polymerise in the presence of AcP in aqueous solution. We conclude that AcP can phosphorylate biologically meaningful substrates in a manner analogous to ATP, promoting the origins of metabolism, but is unlikely to have driven polymerization of macromolecules such as polypeptides or RNA in free solution. This is consistent with the idea that a period of monomer (cofactor) catalysis preceded the emergence of polymeric enzymes or ribozymes at the origin of life.