Ana Paula Carvalho Reis

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Faculdade de Medicina da Universidade de São Paulo - FMUSP

Ana Paula Carvalho Reis


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https://researchid.co/anareis
7

Scopus Publications

82

Scholar Citations

5

Scholar h-index

4

Scholar i10-index

Scopus Publications

  • First case of Trichophyton indotineae in Brazil: clinical and mycological criteria and genetic identification of terbinafine resistance
    John Verrinder Veasey, Renata Diniz Jacques Gonçalves, Guilherme Camargo Julio Valinoto, Gustavo de Sá Menezes Carvalho, Giovanna Azevedo Celestrino, Ana Paula Carvalho Reis, Ana Paula Cordeiro Lima, Antonio Charlys da Costa, Marcia de Souza Carvalho Melhem, Gil Benard, Maria Gloria Teixeira Sousa
    Anais Brasileiros De Dermatologia, 2025
    Brazil with dermatophytosis caused by terbinafine-resistant T. indotineae.A 40-year-old male individual, without comorbidities, originally from Brazil and living in London, reported pruritic erythematous-desquamative lesions on the lower limbs and buttocks that began in January 2024 (Fig. 1).He reported frequent short trips during the second half of 2023; to Austria, Slovakia, Hungary, and Poland in August, and to Scotland and Turkey in November and December.He sought dermatological care in Piracicaba (state of So Paulo, Brazil), where direct mycological examination revealed the presence of hyaline septate hyphae, and the culture using Sabouraud agar and Mycosel showed growth of T. mentagrophytes.He was diagnosed with dermatophytosis and in March 2024 he was prescribed 500 mg/day of terbinafine for 14 days.Although there was no clinical improvement, the patient returned to London; in May 2024 he came back presenting lesions in the same locations (Fig. 2).
  • Optimized protein extraction protocol from human skin samples
    Ana Paula Carvalho Reis, Giovanna Azevedo Celestrino, Talita Souza Siqueira, Milena De Melo Scarano Coelho, Juliana Carreiro Avila, Isabela De Oliveira Cavalcante Pimentel, Leo Kei Iwai, Pritesh Jaychand Lalwani, Vitor Manoel Silva Dos Reis, Kaique Arriel, José Ângelo Lindoso, Gil Benard, Maria Gloria Teixeira Sousa
    Biology Methods and Protocols, 2025
    The skin is the largest organ in the body and is the site for a diverse set of diseases. Yet, given the complexity of the cutaneous tissue, there is a limited availability of data in the literature on skin proteomics. Here, we proposed an adapted and optimized protocol for the extraction of proteins from human skin, using a combination of chemical and mechanical lysis approaches. For this, we used of a lysis buffer containing 2% SDS, 50 mM TEAB, and a 1% protease and phosphatase inhibitor cocktail, in addition to Matrix A beads and a FastPrep-24 5G homogenizer. For the characterization of the samples, the obtained proteins were purified and digested using the SP3 method (Single-pot, solid phase, sample preparation), and analyzed by nano liquid chromatography coupled with tandem mass spectrometry. In this way, we were able to identify around 6000 proteins in the skin samples from healthy individuals and patients with the fungal infection sporotrichosis. Our improved methodology could significantly enrich our understanding of skin biology and provide new perspectives for the discovery of biomarkers and therapeutic targets for cutaneous diseases.
  • In Situ Expression of TNF-α and IL-10 in Human Dermatophytosis Lesions due to Trichophyton rubrum
    Ana Paula Carvalho Reis, Franciele Fernandes Correia, Giovanna Azevedo Celestrino, Carla Pagliari, Paulo Ricardo Criado, Pritesh Jaychand Lalwani, Gil Benard, Maria Gloria Teixeira Sousa
    Mycopathologia, 2024
  • Evidence of Clinical and Laboratory Correlation of Itraconazole Resistance in Sporothrix brasiliensis Infection: Case Report
    John Verrinder Veasey, Ana Paula Carvalho Reis, Giovanna Azevedo Celestrino, Camila Estacia Silva, Eduarda Souza Santos, Denise Polizel Mendes, Tania Sueli Andrade, Lucas Xavier Bonfietti, Gil Benard, Maria Glória Texeira Sousa
    Microorganisms, 2024
    Sporotrichosis is a subcutaneous infection caused by fungi from the genus Sporothrix, among which Sporothrix brasiliensis displays high virulence and transmissibility. So far, classical antifungal agents have been efficient against S. brasiliensis, but here we describe the first case of therapeutic failure and a high minimum inhibitory concentration (MIC) in relation to itraconazole.
  • Rhino-orbito-cerebral mucormycosis caused by Rhizopus microsporus var. microsporus in a diabetic patient with COVID-19
    Sandy Daniele Munhoz, Rute Facchini Lellis, Ana Paula Carvalho Reis, Gilda Maria Barbaro Del Negro, Maria Glória Teixeira Sousa, John Verrinder Veasey
    Anais Brasileiros De Dermatologia, 2022
    COVID-19 disease caused by the SARS-CoV-2 coronavirus causes a wide range of clinical manifestations, ranging from mild to severe, with the main ones affecting the respiratory tract, such as pneumonia. In patients with greater severity, the high frequency of bacterial and fungal coinfection stands out, a situation related both to the patient's pre-existing comorbidities and due to the hospitalization itself. Cases of mucormycosis associated with COVID-19 were highlighted in the lay and scientific media, with the increase in mycosis cases being directly and indirectly attributed to the viral infection. This report describes a case of rhino-orbito-cerebral mucormycosis in a diabetic patient hospitalized for COVID-19, whose diagnosis was confirmed by identifying the agent Rhizopus microsporus var. microsporus through culture for fungi and PCR examination.
  • The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
    Ana Paula Carvalho Reis, Giovanna Azevedo Celestrino, Mariana Villas Bôas Igoa, Thais Martins Jesus, Tábata Takahashi França, Daniel Valério Silva Moreira, Paula Ordonhez Rigato, Paula Keiko Sato, Antonio Condino-Neto, Irene L. Noronha, Luciane Alarcão Dias-Melicio, Pritesh Jaychand Lalwani, Gil Benard, Maria Gloria Teixeira Sousa
    Journal of Fungi, 2022
    Neutrophils are the first leukocytes recruited to the site of infection and are thought to be responsible for fungal elimination from the skin such as dermatophytes. Neutrophils are able to secrete reactive oxygen species (ROS) and neutrophil extracellular traps (NETs) that can kill different fungi, including Aspergillus, spp., Candida albicans, and Phialophora verrucosa. However, NET production in response to Trichophyton rubrum, the main etiologic agent of dermatophytosis, has yet to be studied. We demonstrated that human neutrophils produce NETs against different morphotypes of T. rubrum in a dose-dependent manner and NET formation is dependent on ROS production. In addition, ROS production by human neutrophils in response to T. rubrum is dependent on NADPH oxidase, but not on fungal viability. NETs mediated killing of T. rubrum. Collectively, these results demonstrate that T. rubrum was able to trigger the production of NETs, suggesting that these extracellular structures may represent an important innate immune effector mechanism controlling physiological response to T. rubrum infection.
  • Trichophyton rubrum Elicits Phagocytic and Pro-inflammatory Responses in Human Monocytes Through Toll-Like Receptor 2
    Giovanna Azevedo Celestrino, Ana Paula Carvalho Reis, Paulo Ricardo Criado, Gil Benard, Maria Gloria Teixeira Sousa
    Frontiers in Microbiology, 2019
    Dermatophytosis is a superficial fungal infection mostly restricted to keratinized tissues such as skin, hair, and nails but with potential to cause invasive or even systemic disease in immunocompromised patients. Trichophyton rubrum is the main etiologic agent, accounting for approximately 80% of the cases. Mononuclear phagocytes respond to pathogens through phagocytosis followed by production of several antimicrobial molecules, such as reactive oxygen and nitrogen species, and failure in doing so may contribute to development of chronic fungal infections. Toll-like receptors (TLRs) located on the surface of phagocytic cells bind either directly to target particles or through opsonizing ligands and trigger an actin-mediated ingestion. Even though the mechanisms involved in TLR-mediated cytokine responses are well established, the contribution of TLR in the recognition of T. rubrum by adherent monocytes remains unclear. Here, we report that phagocytosis of T. rubrum conidia by adherent monocytes is mediated by TLR2. Blockade of TLR2 by neutralizing antibodies impaired the fungicidal activity of monocytes as well their secretion of tumor necrosis factor (TNF)-α, but neither nitric oxide (NO) production nor interleukin (IL)-10 secretion was disturbed. So far, our data suggest that TLR2 is required for efficient conidial phagocytosis, and the absence of TLR2 signaling in human monocytes may impair the subsequent inflammatory response. These findings expand our understanding of phagocyte modulation by this important fungal pathogen and may represent a potential target for interventions aiming at enhancing antifungal immune responses.

RECENT SCHOLAR PUBLICATIONS

  • First case of Trichophyton indotineae in Brazil: clinical and mycological criteria and genetic identification of terbinafine resistance
    JV Veasey, RDJ Gonçalves, GCJ Valinoto, GSM Carvalho, GA Celestrino, ...
    Anais Brasileiros de Dermatologia , 2025
    2025
    Citations: 11
  • In Situ Expression of TNF-α and IL-10 in Human Dermatophytosis Lesions due to Trichophyton rubrum
    APC Reis, FF Correia, GA Celestrino, C Pagliari, PR Criado, PJ Lalwani, ...
    Mycopathologia 189 (6), 1-5 , 2024
    2024
    Citations: 2
  • Evidence of Clinical and Laboratory Correlation of Itraconazole Resistance in Sporothrix brasiliensis Infection: Case Report
    JV Veasey, APC Reis, GA Celestrino, CE Silva, ES Santos, DP Mendes, ...
    Microorganisms 12 (11), 2132 , 2024
    2024
    Citations: 5
  • Rhino-orbito-cerebral mucormycosis caused by Rhizopus microsporus var. microsporus in a diabetic patient with COVID-19
    SD Munhoz, RF Lellis, APC Reis, GMBD Negro, MGT Sousa, JV Veasey
    Anais Brasileiros de Dermatologia 97, 501-504 , 2022
    2022
    Citations: 5
  • Mucormicose rino‐órbito‐cerebral causada por Rhizopus microsporus var. microsporus em paciente diabético com COVID‐19
    SD Munhoz, RF Lellis, APC Reis, GMBD Negro, MGT Sousa, JV Veasey
    Anais Brasileiros de Dermatologia (Portuguese) 97 (4), 501-504 , 2022
    2022
  • The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
    APC Reis, GA Celestrino, MVB Igoa, TM Jesus, TT França, DVS Moreira, ...
    Journal of Fungi 8 (2), 147 , 2022
    2022
    Citations: 10
  • The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils. J. Fungi 2022, 8, 147
    APC Reis, GA Celestrino, MVB Igoa, TM Jesus, TT França, DVS Moreira, ...
    s Note: MDPI stays neutral with regard to jurisdictional claims in published … , 2022
    2022
  • Anais Brasileiros de
    SD Munhoz, RF Lellis, APC Reis, GMB Del Negro, MGT Sousa, ...
    2022
  • A case of cutaneous phaeohyphomycosis caused by Biatriospora mackinnonii
    APC Reis, DVS Moreira, GMB Del Negro, AC da Costa, G Benard, ...
    Medical Mycology Case Reports 34, 32-34 , 2021
    2021
    Citations: 3
  • Trichophyton rubrum Elicits Phagocytic and Pro-inflammatory Responses in Human Monocytes Through Toll-Like Receptor 2
    GA Celestrino, APC Reis, PR Criado, G Benard, MGT Sousa
    Frontiers in Microbiology 10, 2589 , 2019
    2019
    Citations: 20
  • In situ immune response in human dermatophytosis: possible role of Langerhans cells (CD1a+) as a risk factor for dermatophyte infection
    APC Reis, FF Correia, TM Jesus, C Pagliari, NY Sakai-Valente, W Belda, ...
    Revista do Instituto de Medicina Tropical de São Paulo 61 , 2019
    2019
    Citations: 26

MOST CITED SCHOLAR PUBLICATIONS

  • In situ immune response in human dermatophytosis: possible role of Langerhans cells (CD1a+) as a risk factor for dermatophyte infection
    APC Reis, FF Correia, TM Jesus, C Pagliari, NY Sakai-Valente, W Belda, ...
    Revista do Instituto de Medicina Tropical de São Paulo 61 , 2019
    2019
    Citations: 26
  • Trichophyton rubrum Elicits Phagocytic and Pro-inflammatory Responses in Human Monocytes Through Toll-Like Receptor 2
    GA Celestrino, APC Reis, PR Criado, G Benard, MGT Sousa
    Frontiers in Microbiology 10, 2589 , 2019
    2019
    Citations: 20
  • First case of Trichophyton indotineae in Brazil: clinical and mycological criteria and genetic identification of terbinafine resistance
    JV Veasey, RDJ Gonçalves, GCJ Valinoto, GSM Carvalho, GA Celestrino, ...
    Anais Brasileiros de Dermatologia , 2025
    2025
    Citations: 11
  • The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils
    APC Reis, GA Celestrino, MVB Igoa, TM Jesus, TT França, DVS Moreira, ...
    Journal of Fungi 8 (2), 147 , 2022
    2022
    Citations: 10
  • Evidence of Clinical and Laboratory Correlation of Itraconazole Resistance in Sporothrix brasiliensis Infection: Case Report
    JV Veasey, APC Reis, GA Celestrino, CE Silva, ES Santos, DP Mendes, ...
    Microorganisms 12 (11), 2132 , 2024
    2024
    Citations: 5
  • Rhino-orbito-cerebral mucormycosis caused by Rhizopus microsporus var. microsporus in a diabetic patient with COVID-19
    SD Munhoz, RF Lellis, APC Reis, GMBD Negro, MGT Sousa, JV Veasey
    Anais Brasileiros de Dermatologia 97, 501-504 , 2022
    2022
    Citations: 5
  • A case of cutaneous phaeohyphomycosis caused by Biatriospora mackinnonii
    APC Reis, DVS Moreira, GMB Del Negro, AC da Costa, G Benard, ...
    Medical Mycology Case Reports 34, 32-34 , 2021
    2021
    Citations: 3
  • In Situ Expression of TNF-α and IL-10 in Human Dermatophytosis Lesions due to Trichophyton rubrum
    APC Reis, FF Correia, GA Celestrino, C Pagliari, PR Criado, PJ Lalwani, ...
    Mycopathologia 189 (6), 1-5 , 2024
    2024
    Citations: 2
  • Mucormicose rino‐órbito‐cerebral causada por Rhizopus microsporus var. microsporus em paciente diabético com COVID‐19
    SD Munhoz, RF Lellis, APC Reis, GMBD Negro, MGT Sousa, JV Veasey
    Anais Brasileiros de Dermatologia (Portuguese) 97 (4), 501-504 , 2022
    2022
  • The Dermatophyte Trichophyton rubrum Induces Neutrophil Extracellular Traps Release by Human Neutrophils. J. Fungi 2022, 8, 147
    APC Reis, GA Celestrino, MVB Igoa, TM Jesus, TT França, DVS Moreira, ...
    s Note: MDPI stays neutral with regard to jurisdictional claims in published … , 2022
    2022
  • Anais Brasileiros de
    SD Munhoz, RF Lellis, APC Reis, GMB Del Negro, MGT Sousa, ...
    2022