Naga Sai Visweswar Kambhampati
@sssihl.edu.in
Assistant Professor, Department of Chemistry
Sri Sathya Sai Institute of Higher Learning
During postdoc as a Research Associate over 3 years, I worked in the field of clinical research with emphasis on development of next-gen immunoassays and mobile phone-based disease diagnostic technologies. During this tenure, I was a member of the core team from Sri Sathya Sai Institute of Higher Learning that developed the SAI C-19 rapid antigen kit for SARS-CoV-2 screening which has been approved by ICMR, INDIA.
EDUCATION
Joined Sri Sathya Sai Institute of Higher Learning (SSSIHL) in 2003 for B.Sc. (Hons.) at Brindavan Campus and went on to complete his M.Sc and M.Phil in Chemistry. Pursued PhD at SSSIHL in the field of bacterial oligosaccharides for their applications with a good exposure to various analytical techniques & instrumentation related to chromatography and spectroscopy.
RESEARCH INTERESTS
Analytical ChemistryBio-assay developmentClinical diagnostic medical device prototypingBioprocessingBioremediation
FUTURE PROJECTS
Development of MOFs for sensing applications
Applications Invited
Collaborators
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Sriram Rathnakumar, Naga Sai Visweswar Kambhampati, R. Saiswaroop, Sai Sanwid Pradhan, G. Ramkumar, Nirmala Beeraka, Gopi Krishna Muddu, Sandeep Kumar, Sai Kiran Javvaji, Ashish Parangoankar,et al.
Springer Science and Business Media LLC
Bebeto Rai, Robert Malmberg, Venkatesh Srinivasan, Kalathur Mohan Ganesh, Naga Sai Visweswar Kambhampati, Abhay Andar, Govind Rao, Carani B. Sanjeevi, Koushik Venkatesan, and Sai Sathish Ramamurthy
American Chemical Society (ACS)
The outbreak of the COVID-19 pandemic has had a major impact on the health and well-being of people with its long-term effect on lung function and oxygen uptake. In this work, we present a unique approach to augment the phosphorescence signal from phosphorescent gold(III) complexes based on a surface plasmon-coupled emission platform and use it for designing a ratiometric sensor with high sensitivity and ultrafast response time for monitoring oxygen uptake in SARS-CoV-2-recovered patients. Two monocyclometalated Au(III) complexes, one having exclusively phosphorescence emission (λPL = 578 nm) and the other having dual emission, fluorescence (λPL = 417 nm) and phosphorescence (λPL = 579 nm), were studied using the surface plasmon-coupled dual emission (SPCDE) platform for the first time, which showed 27-fold and 17-fold enhancements, respectively. The latter complex having the dual emission was then used for the fabrication of a ratiometric sensor for studying the oxygen quenching of phosphorescence emission with the fluorescence emission acting as an internal standard. Low-cost poly (methyl methacrylate) (PMMA) and biodegradable wood were used to fabricate the microfluidic chips for oxygen monitoring. The sensor showed a high sensitivity with a limit of detection ∼ 0.1%. Furthermore, real-time oxygen sensing was carried out and the response time of the sensor was calculated to be ∼0.2 s. The sensor chip was used for monitoring the oxygen uptake in SARS-CoV-2-recovered study participants, to assess their lung function post the viral infection.
Sriram Rathnakumar, Seemesh Bhaskar, Aayush Rai, Darisi V. V. Saikumar, Naga Sai Visweswar Kambhampati, Venketesh Sivaramakrishnan, and Sai Sathish Ramamurthy
American Chemical Society (ACS)
Seemesh Bhaskar, Naga Sai Visweswar Kambhampati, K. M. Ganesh, Mahesh Sharma P, Venkatesh Srinivasan, and Sai Sathish Ramamurthy
American Chemical Society (ACS)
The quest for auxiliary plasmonic materials with lossless properties began in the past decade. In the current study, a unique plasmonic response is demonstrated from a stratified high refractive index (HRI)-graphene oxide (GO) and low refractive index (LRI)-polymethyl methacrylate (PMMA) multistack. Graphene oxide plasmon-coupled emission (GraPE) reveals the existence of strong surface states on the terminating layer of the photonic crystal (PC) framework. The chemical defects in GO thin film are conducive for unraveling plasmon hybridization within and across the multistack. We have achieved a unique assortment of metal-dielectric-metal (MDM) ensuing a zero-normal steering emission on account of solitons as well as directional GraPE. This has been theoretically established and experimentally demonstrated with a metal-free design. The angle-dependent reflectivity plots, electric field energy (EFI) profiles, and finite-difference time-domain (FDTD) analysis from the simulations strongly support plasmonic modes with giant Purcell factors (PFs). The architecture presented prospects for the replacement of metal-dependent MDM and surface plasmon-coupled emission (SPCE) technology with low cost, easy to fabricate, tunable soliton [graphene oxide plasmon-coupled soliton emission (GraSE)], and plasmon [GraPE] engineering for diverse biosensing applications. The superiority of the GraPE platform for achieving 1.95 pg mL-1 limit of detection of human IFN-γ is validated experimentally. A variety of nanoparticles encompassing metals, intermetallics, rare-earth, and low-dimensional carbon-plasmonic hybrids were used to comprehend PF and cavity hot-spot contribution resulting in 900-fold fluorescence emission enhancements on a lossless substrate, thereby opening the door to unique light-matter interactions for next-gen plasmonic and biomedical technologies.
K. N. S. Visweswar, A. Sunil, A. Sri Harsha, and Ch. Janardhana
Wiley
Cyclic β-(1→3),(1→6) glucans (CBGs) isolated from Bradyrhizobium japonicum bacteria are the periplasmic oligosaccharides having cyclic structures. This paper presents the isolation of CBGs from the bacteria cultured using optimized medium that improved yields to 350-450 mg per gram of cellular dry weight along with analytical interaction with lead(II) ions in the range 33.0-2.0 ppm with CBG as a binding ligand, using constant wavelength synchronous fluorescence spectroscopy. The binding ability of CBGs towards lead(II) is clearly evident from the scanning electron microscopy (SEM) images. The theoretical calculations using HEX 8.0 gave an insight about the interaction between CBG and lead(II) to be in the ratio of 3:1. The method displayed the sensitivity and selectivity towards lead(II) ions up to 2.0 ppm. This observed property of CBGs can potentially hold an application in bioremediation of the soil contaminated with lead.
Naga Sai Visweswar Kambhampati, Swayamsiddha Kar, Sai Siva Kumar Pinnepalli, Janardhana Chelli, and Mukesh Doble
Elsevier BV