Naga Sai Visweswar Kambhampati

@sssihl.edu.in

Assistant Professor, Department of Chemistry
Sri Sathya Sai Institute of Higher Learning

During postdoc as a Research Associate over 3 years, I worked in the field of clinical research with emphasis on development of next-gen immunoassays and mobile phone-based disease diagnostic technologies. During this tenure, I was a member of the core team from Sri Sathya Sai Institute of Higher Learning that developed the SAI C-19 rapid antigen kit for SARS-CoV-2 screening which has been approved by ICMR, INDIA.

EDUCATION

Joined Sri Sathya Sai Institute of Higher Learning (SSSIHL) in 2003 for B.Sc. (Hons.) at Brindavan Campus and went on to complete his M.Sc and M.Phil in Chemistry. Pursued PhD at SSSIHL in the field of bacterial oligosaccharides for their applications with a good exposure to various analytical techniques & instrumentation related to chromatography and spectroscopy.

RESEARCH INTERESTS

Analytical ChemistryBio-assay developmentClinical diagnostic medical device prototypingBioprocessingBioremediation

FUTURE PROJECTS

Scopus Publications

161

Scholar Citations

Scholar h-index

Scholar i10-index

Scopus Publications

Tecoma stans Floral Extract-Based Biosynthesis for Enhanced Surface Plasmon-Coupled Emission and a Preliminary Study on Fluoroimmunoassay
Sriram Rathnakumar, Seemesh Bhaskar, Venketesh Sivaramakrishnan, Naga Sai Visweswar Kambhampati, Venkatesh Srinivasan, and Sai Sathish Ramamurthy
American Chemical Society (ACS)
We demonstrate the synthesis of biogenic supported silver spiked star architectures and their application to increase the electromagnetic field intensity at its tips that enhance plasmon-coupled emission. Tecoma stans floral extract has been used to synthesize silver nanocubes and spiked stars. We observe ∼445-fold and ∼680-fold enhancements in spacer and cavity configurations, respectively, in the SPCE platform. The hotspot intensity and Purcell factor are evaluated by carrying out finite-difference time-domain (FDTD) simulations. Time-based studies are presented to modulate the sharpness of the edges wherein an increase in the tip sharpness with the increase in reaction time up to 5 h is observed. The unique morphology of the silver architectures allowed us to utilize them in biosensing application. A SPCE-based fluoroimmunoassay was performed, achieving a 1.9 pg/mL limit of detection of TNF-α cytokine. This combination of anisotropic architectures, SPCE and immunoassay prove to be a powerful platform for the ultrasensitive detection of biomarkers in surface-bound assays.

Nanoengineering at functional plasmonic interfaces
Seemesh Bhaskar, Kalathur Mohan Ganesh, Deepshikha Arora, Yashika Gupta, Bala Gopal Maddala, Attila Bonyár, Walid Tawfik, Sai Sathish Ramamurthy, and Naga Sai Visweswar Kambhampati
Elsevier

Integrated clinical and metabolomic analysis of dengue infection shows molecular signatures associated with host-pathogen interaction in different phases of the disease
Sriram Rathnakumar, Naga Sai Visweswar Kambhampati, R. Saiswaroop, Sai Sanwid Pradhan, G. Ramkumar, Nirmala Beeraka, Gopi Krishna Muddu, Sandeep Kumar, Sai Kiran Javvaji, Ashish Parangoankar,et al.
Springer Science and Business Media LLC

Surface Plasmon-Coupled Dual Emission Platform for Ultrafast Oxygen Monitoring after SARS-CoV-2 Infection
Bebeto Rai, Robert Malmberg, Venkatesh Srinivasan, Kalathur Mohan Ganesh, Naga Sai Visweswar Kambhampati, Abhay Andar, Govind Rao, Carani B. Sanjeevi, Koushik Venkatesan, and Sai Sathish Ramamurthy
American Chemical Society (ACS)
The outbreak of the COVID-19 pandemic has had a major impact on the health and well-being of people with its long-term effect on lung function and oxygen uptake. In this work, we present a unique approach to augment the phosphorescence signal from phosphorescent gold(III) complexes based on a surface plasmon-coupled emission platform and use it for designing a ratiometric sensor with high sensitivity and ultrafast response time for monitoring oxygen uptake in SARS-CoV-2-recovered patients. Two monocyclometalated Au(III) complexes, one having exclusively phosphorescence emission (λPL = 578 nm) and the other having dual emission, fluorescence (λPL = 417 nm) and phosphorescence (λPL = 579 nm), were studied using the surface plasmon-coupled dual emission (SPCDE) platform for the first time, which showed 27-fold and 17-fold enhancements, respectively. The latter complex having the dual emission was then used for the fabrication of a ratiometric sensor for studying the oxygen quenching of phosphorescence emission with the fluorescence emission acting as an internal standard. Low-cost poly (methyl methacrylate) (PMMA) and biodegradable wood were used to fabricate the microfluidic chips for oxygen monitoring. The sensor showed a high sensitivity with a limit of detection ∼ 0.1%. Furthermore, real-time oxygen sensing was carried out and the response time of the sensor was calculated to be ∼0.2 s. The sensor chip was used for monitoring the oxygen uptake in SARS-CoV-2-recovered study participants, to assess their lung function post the viral infection.

Plasmon-Coupled Silver Nanoparticles for Mobile Phone-Based Attomolar Sensing of Mercury Ions
Sriram Rathnakumar, Seemesh Bhaskar, Aayush Rai, Darisi V. V. Saikumar, Naga Sai Visweswar Kambhampati, Venketesh Sivaramakrishnan, and Sai Sathish Ramamurthy
American Chemical Society (ACS)

Metal-Free, Graphene Oxide-Based Tunable Soliton and Plasmon Engineering for Biosensing Applications
Seemesh Bhaskar, Naga Sai Visweswar Kambhampati, K. M. Ganesh, Mahesh Sharma P, Venkatesh Srinivasan, and Sai Sathish Ramamurthy
American Chemical Society (ACS)
The quest for auxiliary plasmonic materials with lossless properties began in the past decade. In the current study, a unique plasmonic response is demonstrated from a stratified high refractive index (HRI)-graphene oxide (GO) and low refractive index (LRI)-polymethyl methacrylate (PMMA) multistack. Graphene oxide plasmon-coupled emission (GraPE) reveals the existence of strong surface states on the terminating layer of the photonic crystal (PC) framework. The chemical defects in GO thin film are conducive for unraveling plasmon hybridization within and across the multistack. We have achieved a unique assortment of metal-dielectric-metal (MDM) ensuing a zero-normal steering emission on account of solitons as well as directional GraPE. This has been theoretically established and experimentally demonstrated with a metal-free design. The angle-dependent reflectivity plots, electric field energy (EFI) profiles, and finite-difference time-domain (FDTD) analysis from the simulations strongly support plasmonic modes with giant Purcell factors (PFs). The architecture presented prospects for the replacement of metal-dependent MDM and surface plasmon-coupled emission (SPCE) technology with low cost, easy to fabricate, tunable soliton [graphene oxide plasmon-coupled soliton emission (GraSE)], and plasmon [GraPE] engineering for diverse biosensing applications. The superiority of the GraPE platform for achieving 1.95 pg mL-1 limit of detection of human IFN-γ is validated experimentally. A variety of nanoparticles encompassing metals, intermetallics, rare-earth, and low-dimensional carbon-plasmonic hybrids were used to comprehend PF and cavity hot-spot contribution resulting in 900-fold fluorescence emission enhancements on a lossless substrate, thereby opening the door to unique light-matter interactions for next-gen plasmonic and biomedical technologies.

Interaction studies of lead(II) ion with cyclic β-(1→3),(1→6) glucans extracted from Bradyrhizobium japonicum based on ‘chelation enhanced fluorescence’ (CHEF) effect
K. N. S. Visweswar, A. Sunil, A. Sri Harsha, and Ch. Janardhana
Wiley
Cyclic β-(1→3),(1→6) glucans (CBGs) isolated from Bradyrhizobium japonicum bacteria are the periplasmic oligosaccharides having cyclic structures. This paper presents the isolation of CBGs from the bacteria cultured using optimized medium that improved yields to 350-450 mg per gram of cellular dry weight along with analytical interaction with lead(II) ions in the range 33.0-2.0 ppm with CBG as a binding ligand, using constant wavelength synchronous fluorescence spectroscopy. The binding ability of CBGs towards lead(II) is clearly evident from the scanning electron microscopy (SEM) images. The theoretical calculations using HEX 8.0 gave an insight about the interaction between CBG and lead(II) to be in the ratio of 3:1. The method displayed the sensitivity and selectivity towards lead(II) ions up to 2.0 ppm. This observed property of CBGs can potentially hold an application in bioremediation of the soil contaminated with lead.

Microbial cyclic β-(1 → 3),(1 → 6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid
Naga Sai Visweswar Kambhampati, Swayamsiddha Kar, Sai Siva Kumar Pinnepalli, Janardhana Chelli, and Mukesh Doble
Elsevier BV

RECENT SCHOLAR PUBLICATIONS

Host-Guest Inclusion Complexes of Tafamidis with β-Cyclodextrin: Preparation, Characterization, and In Vitro Antibacterial and Antioxidant Approach
N Swarnkar, AS Kamalakaran, J Chintha, NSV Kambhampati, L Sripada, ...
Journal of Molecular Structure, 141649 2025

Nanoengineering at functional plasmonic interfaces
S Bhaskar, KM Ganesh, D Arora, Y Gupta, BG Maddala, A Bonyr, ...
Nano-Engineering at Functional Interfaces for Multi-Disciplinary 2025

Tecoma stans Floral Extract-Based Biosynthesis for Enhanced Surface Plasmon-Coupled Emission and a Preliminary Study on Fluoroimmunoassay
S Rathnakumar, S Bhaskar, V Sivaramakrishnan, NSV Kambhampati, ...
Analytical Chemistry 96 (10), 4005-4012 2024

Integrated clinical and metabolomic analysis of dengue infection shows molecular signatures associated with host-pathogen interaction in different phases of the disease
S Rathnakumar, NSV Kambhampati, R Saiswaroop, SS Pradhan, ...
Metabolomics 19 (5), 47 2023

Surface plasmon-coupled dual emission platform for ultrafast oxygen monitoring after sars-cov-2 infection
B Rai, R Malmberg, V Srinivasan, KM Ganesh, NSV Kambhampati, ...
ACS sensors 6 (12), 4360-4368 2021

Plasmon-coupled silver nanoparticles for mobile phone-based attomolar sensing of mercury ions
S Rathnakumar, S Bhaskar, A Rai, DVV Saikumar, NSV Kambhampati, ...
ACS Applied Nano Materials 4 (8), 8066-8080 2021

Metal-free, graphene oxide-based tunable soliton and plasmon engineering for biosensing applications
S Bhaskar, NS Visweswar Kambhampati, KM Ganesh, MS P, V Srinivasan, ...
ACS Applied Materials & Interfaces 13 (14), 17046-17061 2021

P, MS; Srinivasan, V.; Ramamurthy, SS Metal-Free, Graphene Oxide-Based Tunable Soliton and Plasmon Engineering for Biosensing Applications
S Bhaskar, NS Visweswar Kambhampati, KM Ganesh
ACS Appl. Mater. Interfaces 13, 17046-17061 2021

Interaction studies of lead(II) ion with cyclic β‐(1→3),(1→6) glucans extracted from Bradyrhizobium japonicum based on ‘chelation enhanced fluorescence’ (CHEF
KNS Visweswar, A Sunil, A Sri Harsha, C Janardhana
Luminescence 33 (7), 1202-1208 2018

Microbial cyclic β-(1→ 3),(1→ 6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid
NSV Kambhampati, S Kar, SSK Pinnepalli, J Chelli, M Doble
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 203, 494-500 2018

Sensing Applications of Cyclic β-(1→3),(1→6)-Glucans produced by Bradyrhizobium japonicum
KNS Visweswar, A Sunil, C Janardhana
National Conference on “Frontiers in Chemical Sciences &Technologies” at 2016

Isolation and Preliminary Studies on Characterization of Cyclic β-(1→3), (1→6)-Glucans produced by Bradyrhizobium japonicum
KNS Visweswar, C Janardhana
UK-India MedChem Congress 2013 organized at Indian Institute of Chemical 2013

MOST CITED SCHOLAR PUBLICATIONS

Microbial cyclic β-(1→ 3),(1→ 6)-glucans as potential drug carriers: Interaction studies between cyclic β-glucans isolated from Bradyrhizobium japonicum and betulinic acid
NSV Kambhampati, S Kar, SSK Pinnepalli, J Chelli, M Doble
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 203, 494-500 2018
Citations: 10

Nanoengineering at functional plasmonic interfaces
S Bhaskar, KM Ganesh, D Arora, Y Gupta, BG Maddala, A Bonyr, ...
Nano-Engineering at Functional Interfaces for Multi-Disciplinary 2025
Citations: 2

Naga Sai Visweswar Kambhampati

EDUCATION

RESEARCH INTERESTS

FUTURE PROJECTS

Development of MOFs for sensing applications

Scopus Publications

RECENT SCHOLAR PUBLICATIONS

MOST CITED SCHOLAR PUBLICATIONS