Dr. Ashish Warghane, completed his Bachelor of Science, Master of Science in Biotechnology from Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur and Ph.D in Biotechnology from SHUATS Prayagraj. He has ten years teaching and research experience in the field of Plant Virology & Molecular Diagnostics. He is principally working on the development of novel molecular diagnostic tools and control of plant viruses and virus like pathogens. He published more than 20 research articles in Scopus indexed, peer reviewed international and national journals, like Phytopathology (American Phytopathological Society), PlosOne, Journal of Virological Methods (Elsevier), Phytopathology, Plant Disease (American Phytopathological Society), Phytoparasitica (Springer), Biochimica et Biophysica Acta (Elsevier), Australasian plant pathology journal, Journal of Plant Biochemistry and Biotechnology (Springer). He also published Book chapter in Springer, Elsevier & Bentham. He is an editor of Agrifood Magazi
Isolation and Characterization of Major Cultivable Bacteria from Novel Natural Fertilizer with Comprehensive Nutrient Analysis Ashish Warghane, Jigisha Thakkar, Gayatary Bhardwaj, Vaibhav Bhatt, B.A. Chopade Journal of Pure and Applied Microbiology, 2025 Ancient Natural Fertilizer (Jivamrit) is a traditional liquid natural mixture that undergoes fermentation and is frequently applied to enhance the soil microbiome. It is a great source of biomass with macro and micronutrients needed by crops, as well as increasing the organic carbon in the soil. With a thorough nutritional analysis that included bacterial isolation, biochemical tests, and bacterial identification by 16S rRNA sequencing, and nutrient analysis of Jivamrit, the current study concentrated on the isolation along with characterization of the major culturable bacteria from Jivamrit prepared using Gir cow native to Gujarat, India. Jivamrit yielded a total of fifteen major isolates, which were utilized for biochemical analysis and morphological evaluation. The biochemical analysis (catalase, indole synthesis, methyl red, VP test, urease, citrate, sucrose fermentation) and plant growth-promoting assays were performed on all isolated bacteria. The chemical analysis of Jivamrit revealed that it contains large amounts of macro and micronutrients and, importantly, contains Azotobacter, Zinc solubilizing bacteria, and total potash mobilizing bacteria. Molecular characterization of bacterial isolates was performed by amplifying the 16S rRNA gene using bacterial universal primers. PCR-amplified product was sequenced and was carried out, and its analysis revealed that the majority of isolates belong to the Bacillus and Priestia genera. A phylogenetic and evolutionary analysis imparts that the majority of isolates belonged to a different Bacillus species, while JW6 and JW9 belonged to Priestia megaterium. Thus, the present study revealed that major cultivable Bacillus and Priestia bacterial genus are present in Jivamrit. A literature survey revealed that the identified species immensely contribute to increasing soil fertility, plant growth, and yield. Therefore, Jivamrit acts as an alternative to chemical manure and works as a natural fertilizer, which minimises the hazardous effects of chemical farming practices on human well-being and the surroundings.
Emerging Nanotechnological Applications in Preserving and Improving the Shelf Life of Food Rashmi Saini, Neha Paserkar, Anand Varma, Dhaval K. Acharya, Vijay Upadhye, et al. Pharmaceutical Nanotechnology, 2025 The ever-growing demand for safe and nutritious food has activated the scrutinization of innovative approaches to enhance food preservation and extended shelf life. Nanotechnology has progressed by making a significant contribution to the food industry at the nanoscale level and appeared as a promising avenue for these challenges. Various nanomaterials have been employed to preserve and extend the shelf life of a variety of food products. Since most harvested fruits and vegetables have a perishable nature, they cannot be preserved in natural circumstances for a long period. Due to a range of unique qualities, nanotechnology-related shelf life extension technologies can compensate for the limitations of normal preservation procedures. The encapsulation of nutraceuticals increases their stability and bioavailability, resulting in beneficial effects on humans. Nanoparticles are used as carriers of health-promoting and/or functional substances in product formulations. They have shown excellent effectiveness in encapsulating bioactive substances and retaining their qualities to ensure their functioning (antioxidant and antibacterial) in food products. This review focuses on the current developments in nanotechnology and their application for improving shelf life and food preservation techniques. Here we excavated the implementation of different types and forms of nanostructured materials (NSMs), from inorganic metals, metal oxides, and their nanocomposites to nano-organic materials incorporating bioactive chemicals in the food system. This review also focuses on exploring the slow and sustainable release of the bioactive compounds, and nutrients enriching the taste and sensory characteristics of the food. Throughout the paper, we dug deep into the regulatory, food safety, and assessment concerns about nanotechnology. The review provides a deep understanding of the developing landscape of nanotechnological applications, challenges, and future opportunities revolutionizing the preservation and extended shelf life of food products.
Evaluating the impact of natural fertilizers on growth dynamics and yield performance of Solanum melongena L. Ashish Warghane, Jigisha Thakkar, Kartik Chauhan, Dolatsinh Zala, Harsh Patel, et al. Frontiers in Sustainable Food Systems, 2025 A one-year field experiment (2023–2024) was laid out in a randomized complete block design (RCBD) with a spacing of 50 × 40 cm involving three replications with five treatments having cow manure along with integrated nutrient management in a plot size of 4.0 m × 1.5 m. The effect of cow manure and mineral fertilizers on soil fertility, nutrient uptake, yield, and farmers’ economic profitability. The experiment was laid out during the Rabi season of the Ahmedabad region. T2 (250 mL of Jivamrit) and T3 (500 mL of Jivamrit) obtained high brinjal production compared to other crops. Also, these two treatment plants contain high organic carbon content, available N, P, K in the soil and more N, P and K uptake by the plants. Further, this treatment obtained the greatest value for yield and net income. The outcomes of this investigation suggested that the combined usage of cow manure prepared Jivamrit with the proper dose gave the best result in the crop and a high crop yield. The present study reveals that Jivamrit would be used as a natural fertilizer for sustainable farming, which minimizes the hazardous effect of chemical fertilizers.
Jivamrit as a Sustainable Approach: A Review of Natural Farming and Future Agriculture Ashish Warghane, Vaibhav Bhatt, B.A. Chopade, Jigisha Thakkar, Ritu Sharma, et al. Recent Advances in Food Nutrition and Agriculture, 2025 Green Revolution aims to boost food production and feed millions of Indians, but it also has negative effects on agriculture and society's health. Natural manures like cow dung and cow urine can counteract the adverse effects of inorganic fertilizer on soil along with improving physicochemical qualities, maintaining the soil quality, and increasing crop output. Zero Budget Natural Farming (ZBNF) formulations like Jivamrit promote soil health and microbial activities and are an excellent source of macronutrients, other micronutrients needed for plant growth, plus adds beneficial microbes, nitrogen (N2), phosphorus (P), potassium (K), and natural carbon (C). Further, conventional agricultural methods, like monocropping and heavy tillage, can damage soil bacteria which contributes to sustainable agriculture through nitrogen fixation, siderophore synthesis and nutrient absorption. A sustainable agricultural system is resource-efficient, socially and commercially competitive, ecologically sound, and supportive of society. Jivamrit, a natural organic manure, is gaining interest due to concerns about the sustainability of input-intensive agriculture systems. It promotes crop growth, quality, and yield, enhances soil pH, population, and activity of beneficial microorganisms, and helps with nitrogen fixation, phosphate solubilization, and easy decomposition. Long-term use of Jivamrit, may disrupt soil microbial balance, may leading to overpopulation of certain species. The current review on the Jivamrit emphasizes on the biological and chemical characterization and its significance to the agriculture.
Genome editing against bacterial plant pathogens Ashish Warghane, Neha G. Paserkar, Sumit Bhose Genome Editing in Bacteria Part 2, 2024 Meeting the crucial demand for sustainable agriculture is an upcoming challenge worldwide, leading to global food security concerns. Approximately 50% of agricultural loss is caused by both biotic and abiotic stresses. As per the estimation of Agrios, 42% of crop loss is characterized by biotic stress alone. Bacteria are the second largest contributor in terms of economic losses caused by various plant diseases. Hence, there is a need to develop elite cultivars in amalgamation with readily available sequenced plant database and progressive genome editing. This has proved to be a groundbreaking/milestone in the field of plant breeding for any desired trait. Until now, many new plant breeding techniques (NPBTs) have been introduced for crop improvement. These techniques include site-specific mutagenesis, cisgenesis, intragenesis, breeding with transgenic inducer lines, etc. This book chapter provides a comparative understanding of enrichment in plant genome editing approach about bacterial pathogens aiming for sustainable agriculture development. This chapter also brings a broad aspect of the application, advantages, unsighted aspects of genome editing, and future challenges.
Citrus Huanglongbing: innovative strategies for sustainable management D Ghosh, R Bharsakale, A Warghane, AK Sharma Indian Phytopathology, 1-6 , 2026 2026
Draft genome sequence CR-NGP1 strain of ‘ Candidatus Liberibacter asiaticus’ ( C Las) from the host Citrus reticulata (Nagpur mandarin) from Central India RD Bharsakale, MG Gubyad, PTK Jagannadham, SB Kokane, ... World Journal of Microbiology and Biotechnology 42 (6), 261 , 2026 2026
Jivaamrit: Harnessing Wisdom of Vrukshaayurveda in Present Era 3 Ashish Warghane1*, Gayatary Bhardwaj1 , Sneha Patel1 , Jigisha Thakkar1 ... Int.J.Curr.Microbiol.App.Sci 14 (10), 239-245 , 2025 2025
Evaluating the impact of natural fertilizers on growth dynamics and yield performance of Solanum melongena L. A Warghane, J Thakkar, K Chauhan, D Zala, H Patel, V Bhatt, ... Frontiers in Sustainable Food Systems 9, 1668080 , 2025 2025
Plant-Based Approach for Nutrient Enhancement and Quality Assurance in Food MN Kher, A Warghane, SP Dholakia, VV Shah, VD Bhatt Sustainable Food Fortification: Biobased Approaches and Strategies, 197-216 , 2025 2025
Impact of salt-induced chemo-signal on rhizomicrobiome-plant interaction M Thakar, B Rajguru, J Thakkar, V Bhatt, M Shri, A Warghane Journal of Crop Health 77 (4), 131 , 2025 2025 Citations: 4
Emerging nanotechnological applications in preserving and improving the shelf life of food R Saini, N Paserkar, A Varma, DK Acharya, V Upadhye, D Pathak, ... Pharmaceutical Nanotechnology 13 (3), 396-410 , 2025 2025 Citations: 13
Sculpting the Harvest: Genomics and Genome Editing Applications for Enhanced Oil Crop Development S Ansari, N Dave, A Warghane, A Sharma, A Gaur, SK Singh, LS Saini Omics and Genome Editing: Revolution in Crop Improvement for Sustainable … , 2025 2025 Citations: 2
Isolation and Characterization of Major Cultivable Bacteria from Novel Natural Fertilizer with Comprehensive Nutrient Analysis. A Warghane, J Thakkar, G Bhardwaj, V Bhatt, BA Chopade Journal of Pure & Applied Microbiology 19 (1) , 2025 2025 Citations: 5
Dialogue in the Dirt: Plant-Microbe A Warghane, MB Vaishnav, LS Saini, R Bawankar, S Gautam, J Thakkar, ... Climate Change and Soil Microorganisms for Environmental Sustainability, 67 , 2025 2025
Jivamrit as a sustainable approach: a review of natural farming and future agriculture A Warghane, V Bhatt, BA Chopade, J Thakkar, R Sharma, A Mondal, ... Recent Advances in Food, Nutrition & Agriculture , 2024 2024 Citations: 2
Genome editing against bacterial plant pathogens A Warghane, NG Paserkar, S Bhose Genome Editing in Bacteria (Part 2), 43-67 , 2024 2024 Citations: 1
Current status of potential antiviral drugs derived from plant, marine, and microbial sources R Saini, MI Ali, M Pant, A Warghane Anti-Infective Agents 22 (2), 61-73 , 2024 2024 Citations: 11
Application of nanoparticles for management of plant viral pathogen: current status and future prospects A Warghane, R Saini, M Shri, I Andankar, DK Ghosh, BA Chopade Virology 592, 109998 , 2024 2024 Citations: 50
Case Report: Burn injury on left hand ulceration and gangrene and debridement “Case report.” U Warghane, R Sharma, A Warghane, P Meshram F1000Research 13, 173 , 2024 2024 Citations: 1
Endophytic fungi: symbiotic bioresource for production of plant secondary metabolites LS Saini, S Patel, A Gaur, P Warghane, R Saini, A Warghane Endophytic fungi: The hidden sustainable jewels for the pharmaceutical and … , 2024 2024 Citations: 4
COVID 19-The Deadliest Pandemic of the Era A Warghane, T Rajguru, S Babar, G Bhardwaj, U Warghane, R Saini, ... EURASIAN JOURNAL OF MEDICAL ADVANCES 4 (3), 131-143 , 2024 2024
Metagenomics for microbial degradation and detoxification of heavy metals BN Rekadwad, AJ Warghane Microbiome-Assisted Bioremediation, 129-137 , 2024 2024 Citations: 1
Integration of phenotypic, qPCR and genome sequencing methodologies for the detection of antimicrobial resistance and virulence in clinical isolates of a tertiary hospital, India M Vohra, M Babariya, JS Parmar, N Kamath, A Warghane, D Zala 3 Biotech 13 (11), 368 , 2023 2023 Citations: 2
Case Report on Acute Cerebrovascular Episode with Pseudobulbar Palsy AJW Utkarsh Manoj Warghane, Ranjana Sharma, Savita Pohekar International Journal of Clinical Case Reports and Reviews 319, 1-5 , 2023 2023
MOST CITED SCHOLAR PUBLICATIONS
Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of "Candidatus Liberibacter asiaticus" MKR Dilip Kumar GhoshID, Sunil B. Kokane, Amol D. Kokane, Ashish J. Warghane ... Plos One 13, 1-24 , 2018 2018 Citations: 105
Antimicrobial nano-zinc oxide-2S albumin protein formulation significantly inhibits growth of “ Candidatus Liberibacter asiaticus” in planta DK Ghosh, S Kokane, P Kumar, A Ozcan, A Warghane, M Motghare, ... PLoS One 13 (10), e0204702 , 2018 2018 Citations: 65
A rapid and sensitive reverse transcription–loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Indian citrus ringspot virus AD Kokane, SB Kokane, AJ Warghane, MG Gubyad, AK Sharma, ... Plant Disease 105 (05), 1346-1355 , 2021 2021 Citations: 63
Application of nanoparticles for management of plant viral pathogen: current status and future prospects A Warghane, R Saini, M Shri, I Andankar, DK Ghosh, BA Chopade Virology 592, 109998 , 2024 2024 Citations: 50
The role of nanocomposites against biofilm infections in humans A Varma, A Warghane, NK Dhiman, N Paserkar, V Upadhye, A Modi, ... Frontiers in Cellular and infection Microbiology 13, 1104615 , 2023 2023 Citations: 50
Loop-mediated isothermal amplification (LAMP) based method for rapid and sensitive detection of ‘Candidatus Liberibacter asiaticus’ in citrus and the psyllid vector, Diaphorina … DK Ghosh, S Bhose, A Warghane, M Motghare, AK Sharma, AK Dhar, ... Journal of plant biochemistry and biotechnology 25 (2), 219-223 , 2016 2016 Citations: 43
Genetic Diversity of the Indian Populations of ‘ Candidatus Liberibacter asiaticus’ Based on the Tandem Repeat Variability in a Genomic Locus DK Ghosh, S Bhose, M Motghare, A Warghane, K Mukherjee, ... Phytopathology 105 (8), 1043-1049 , 2015 2015 Citations: 43
Development of a simple and rapid reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay for sensitive detection of Citrus tristeza virus A Warghane, P Misra, S Bhose, KK Biswas, AK Sharma, MK Reddy, ... Journal of Virological Methods 250, 6-10 , 2017 2017 Citations: 40
Quantitative distribution of Citrus yellow mosaic badnavirus in sweet orange (Citrus sinensis) and its implication in developing disease diagnostics M Motghare, AK Dhar, A Kokane, A Warghane, S Kokane, AK Sharma, ... Journal of virological methods 259, 25-31 , 2018 2018 Citations: 21
Crystal structure analysis in Zn2+-bound state and biophysical characterization of CLas-ZnuA2 N Sharma, P Selvakumar, G Saini, A Warghane, DK Ghosh, AK Sharma Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1864 (12), 1649-1657 , 2016 2016 Citations: 20
Molecular detection and coat protein gene based characterization of Citrus tristeza virus prevalent in Sikkim state of India A Warghane, A Kokane, S Kokane, M Motghare, D Surwase, ... Indian Phytopathology 73 (1), 135-143 , 2020 2020 Citations: 19
First report of a ‘Candidatus Phytoplasma cynodontis’-related strain (group 16SrXIV) associated with Huanglongbing disease on Citrus grandis DKGMMAKSKAWSBDSMS Ladaniya Australasian Plant Disease Notes 14 (9), 1-4 , 2019 2019 Citations: 19
In-silico characterization and RNA-binding protein based polyclonal antibodies production for detection of citrus tristeza virus SB Kokane, AD Kokane, P Misra, AJ Warghane, P Kumar, MG Gubyad, ... Molecular and cellular probes 54, 101654 , 2020 2020 Citations: 17
Development of a real-time RT-PCR method for the detection of Citrus tristeza virus (CTV) and its implication in studying virus distribution in planta SB Kokane, P Misra, AD Kokane, MG Gubyad, AJ Warghane, D Surwase, ... 3 Biotech 11 (10), 431 , 2021 2021 Citations: 16
First report of a 16SrXIV group phytoplasma associated with witches’-broom disease of acid lime (Citrus aurantifolia) in India DK Ghosh, S Bhose, P Sharma, A Warghane, M Motghare, MS Ladaniya, ... Plant Dis 101 (5), 831 , 2017 2017 Citations: 16
Molecular characterization of Citrus yellow mosaic badnavirus (CMBV) isolates revealed the presence of two distinct strains infecting citrus in India DK Ghosh, S Bhose, K Mukherjee, B Aglave, AJ Warghane, M Motghare, ... Phytoparasitica 42 (5), 681-689 , 2014 2014 Citations: 16
Isolation and characterization of Pseudomonas species from Godavari river sample AJ Warghane, GN Wagh, B Nag, ML Jisnani, RR Thaware, HS Kitey Asiat J Biotechnol Resour 2 (07), 862-866 , 2011 2011 Citations: 16
Rapid and Sensitive Detection of Citrus tristeza virus Using Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay DK Ghosh, A Warghane, KK Biswas Citrus Tristeza Virus: Methods and Protocols, 143-150 , 2019 2019 Citations: 14
Emerging nanotechnological applications in preserving and improving the shelf life of food R Saini, N Paserkar, A Varma, DK Acharya, V Upadhye, D Pathak, ... Pharmaceutical Nanotechnology 13 (3), 396-410 , 2025 2025 Citations: 13
Diversity and characterization of Citrus tristeza virus and Candidatus Liberibacter asiaticus associated with citrus decline in major citrus growing areas of India PKSDKG Ashish Warghane, P. Misra, D. K. Ghosh (LKN) Indian Phytopathology 70 (3), 359-367 , 2017 2017 Citations: 12
GRANT DETAILS
Name of Project: Molecular detection and characterization of ‘Candidatus Liberibacter from major citrus growing region of Madhya Pradesh
Funding agency: Mandsaur University Research Council, (MURC) Mandsaur
Cost: Rs. 50000.00
Status: Completed
Name of Project: Production of organic manure using Soyabean industrial waste
Funding agency: Innovation, Incubation & Intellectual Property Rights Cell (III Cell), Mandsaur University
Cost: Rs. 28000.00
status: Completed