Chaithanya BH
@angrau.ac.in
EDUCATION
PhD in Plant Pathology
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B.H. Chaithanya, T. Srinivas, and B.V. Bhaskara Reddy
Agricultural Research Communication Center
Background: Chilli is an important spice and vegetable crop grown in tropical and sub-tropical climate. Recently, Chilli leaf curl disease has become a serious threat to chilli growing farmers. Methods: The present study was aimed to know prevalence of leaf curl disease in chilli. For this, roving field survey was carried out in 63 randomly selected chilli fields covering 21 villages in seven mandals of Kurnool district in Andhra Pradesh during 2019-20. Attempts were also made on molecular diagnosis of begomovirus associated with chilli leaf curl disease. Result: The average mean per cent incidence of chilli leaf curl virus in Kurnool district was 50.76% ranging from 25.0% to 82.2%. During the field survey, the highest mean leaf curl incidence was noticed in Peddakadubur mandal (71.1%) followed by Gonegandla (59.96%) and Nandavaram (52.27%) mandals, while the lowest leaf curl incidence was observed at Adoni (37.83%) mandal in Kurnool district. The amplified coat protein gene was sequenced and obtained 770bp sequence which was deposited in Gen Bank. The obtained sequence shared maximum identity of 99.7% with chilli leaf curl virus Guntur isolate (MN417112). As per species demarcation and nomenclature criteria of begomovirus, the virus associated with chilli leaf curl disease in Kurnool district shared greater than 94% sequence identity with Chilli leaf curl virus. Hence it was identified as chilli leaf curl virus (ChiLCV).
M. Jayalakshmi, G. Prasad Babu, D. Lakshmi Kalyani, and B.H. Chaithanya
Agricultural Research Communication Center
Background: Onfarm Testings were conducted to asses effect of TNAU Pulse wonder in pigeonpea variety LRG-52 in ten farmer’s holdings of kurnool district during kharif from 2018-19 to 2020-21. Methods: The experiment consisted of 7 technology options (treatments). T1: consisting recommended dose of fertilizers along with spraying of pulse wonder @ 5 kg ha-1 during flowering and 15 days after 1st spray, T2: 75% RDF + Pulse wonder @ 5 kg ha-1 at flowering, T3: 50% RDF + Pulse wonder 5 kg ha-1 at flowering,T4: 100% RDF+ 2% DAP at flowering and pod formation stage T5: 100% RDF alone (20:50: 0 NPK kg ha-1),T6: Farmers practice ( 30: 60: 10 NPK kg ha-1 ),T7: Control. Data was analyzed using least significant difference in R software. Result: The results revealed that the T1 recorded the highest plant height (130,129,127 cm at flowering stage in 2018-19, 2019-20 and 2020-21 respectively). The lowest flower shedding observed in T1 treatment while it was the highest under T6 in all the three years of the study. Pod yield was the highest in T1 (1889, 1870 and ,1873 kg ha-1 in 2018-19, 2019-2020 and 2020-21 respectively) and the lowest in T7. The average highest benefit cost ratio (4.0) was realized in T1 and lowest in farmers practice (T6). Seed yield was positively correlated with number of flowers plant -1 (r value 0.813, 0.762 and 0.735) and test weight (r value 0.795,0.8 and 0.754) and negatively correlated with flower shedding ( r value -0.788,-0.712 and -0.750) in 2018-19, 2019-20 and 2020-21 years respectively. The final yield of crop is the cumulative effects of growth and yield attributes which manipulate the favorable parameters for higher yields.
B.H. Chaithanya, B.V. Bhaskara Reddy, L. Prasanthi, R. Sarada Jayalakshmi Devi, K. Manjula, and G. Mohan Naidu
Agricultural Research Communication Center
Background: Vigna mungo (Blackgram) is the major pulse crop cultivated in the Indian sub-continent. It is highly prone to yellow mosaic disease (YMD), which is widespread problem because all cultivated varieties are susceptible to the YMD in Andhra Pradesh state. The presence of two species of begomovirus i.e. Mungbean yellow mosaic virus (MYMV) and Mungbean yellow mosaic India virus (MYMIV) with YMD was reported in Andhra Pradesh. The current study was aimed to develop simple method of viral dimer clone construction and identifying the resistant blackgram genotypes against two species of begomovirus (MYMV and MYMIV) by agroinoculation technique. Methods: In this study, we standardize the rolling circle amplification (RCA) based viral dimer clone construction (MYMV DNA-A, MYMV DNA-B and MYMIV DNA-A) and constructed viral dimer clones were used to transform Agrobacterium tumefaciens EHA105 cells through freeze-thaw technique. Sprouted seed method of agroinoculation screening was conducted. Result: Total 45 blackgram genotypes were screened by sprouted seed method of agroinoculation with two combinations of viral dimeric constructs (MYMV-TPT-A+MYMV-TPT-B and MYMIV-TPT-A+MYMV-TPT-B). Twenty four genotypes offered resistant against MYMV infection and twenty three genotypes were resistant to MYMIV infection. Total 17 genotypes offered resistance to both species of virus (MYMV andMYMIV) associated with YMD.
D. Vijay Kumar Naik, B.V. Bhaskara Reddy, R. Sarada Jayalakshmi Devi, L. Prasanthi, R. Lakshminarayana Vemireddy, Akkari Srividhya, B.H. Chaithanya, T.M. Hemalatha, and K. Sailaja
Agricultural Research Communication Center
Background: The productivity of blackgram is affected by many biotic and abiotic stresses. Among the biotic stresses, yellow mosaic disease (YMD) cause severe yield loss and it is caused by four distinct viruses (belongs to genus begomovirus) collectively known as yellow mosaic virus (YMV). Hence there is need to characterize various YMV isolates associated with blackgram in Andhra Pradesh. Methods: YMV infected blackgram samples were collected from East Godavari, Kurnool and Prakasam districts of Andhra Pradesh. The Rolling Circle Amplification (RCA) based full length MYMIV DNA-A and MYMV DNA-B of three isolates were cloned and sequenced. Result: Nucleotide sequence of full length DNA-A of MYMIV-East Godavari isolate showed greater than 96% similarity at nucleotide and greater than 90% at amino acid level with other MYMIV isolates in NCBI database. The complete DNA-A nucleotide sequence of MYMIV-Kurnool and MYMIV-Prakasam isolates shared greater than 99% similarity at nucleotide and greater than 98% at amino acid level with other MYMIV isolates. The complete nucleotide sequence of MYMV DNA-B of three isolates (East Godavari, Kurnool and Prakasam) had greater than 97% homology with other MYMV DNA-B isolates from database. The predicted amino acid sequence of MYMV DNA-B of three isolates shared greater than 96% homology with other MYMV-B isolates. The common region (CR) sequence similarity between MYMIV-As and MYMV-Bs of East Godavari and Kurnool isolates was 76.4% and 78.3% with Prakasam isolate. The divergence between the MYMIV-A and MYMV-B of present three isolates (East Godavari, Kurnool and Prakasam) under study were ranged from 22.2 to 22.6%.
M. Jayalakshmi, G. Prasad Babu, and B. H. Chaithanya
Agricultural Research Communication Center
Back Ground: Rice (Oryza Sativa L.) is major crop in Andhra Pradesh being cultivated in 22.11 lakh ha average productivity of 3350Kg/ha Local BPT-5204 (samba mashuri) is high susceptibility to blast, sheath blight and gall midge. Since this is consumers’ preference both in the rural and urban markets, farmers are cultivating BPT-5204 though this variety is susceptibility to blast and BPH leads to high cost of cultivation .As an alternative to this NDLR-7 variety developed by Regional Agricultural Research Station (RARS), Nandyal, ANGRAU NDLR-7 was tolerant to blast, BPH, sheath blight, leaf folders and gall midge. Methods: NDLR-7 was introduced by KVK, Banavasi through on farm trail during 2016-17 and this trial was continued for three years as an alternative to traditionally grown BPT-5204. Result: The data from the three years of on farm trail reveals that the cost of cultivation has drastically reduced from Rs. 66050/ha to 50,538/ha due to reduced use of pesticides. The yield in demonstration plot (NDLR-7) was 7437.5 Kg/ha compared to control (5812 Kg/ha).
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