Elsayed

@nrc.sci.eg

Biotechnology Research Institute, Microbial Biotechnology Department
National Research Centre

RESEARCH INTERESTS

Vaccination, Infectious diseases, Immunology, Cancer Research

17

Scopus Publications

Scopus Publications

  • Exploring bee venom and silver nanoparticles for controlling foulbrood pathogen and enhancing lifespan of honeybees
    Sawsan El-Sayied Ali, Gamal El-Ghannam, Mohamed El-Sayied Hashish, Hassan Elsayed, Ahmed Kamel Ali, Wael Mahmoud Marzouk, Ahmed Mahmoud Khattaby, Ashraf M. Abd El-Wahab, Shams H. Abdel-Hafez, and Yasser Attia Attia

    Springer Science and Business Media LLC

  • Assessment of wound healing activity in diabetic mice treated with a novel therapeutic combination of selenium nanoparticles and platelets rich plasma
    Rania A. Karas, Shaimaa Alexeree, Hassan Elsayed, and Yasser A. Attia

    Springer Science and Business Media LLC
    AbstractDiabetic wound healing is sluggish, often ending in amputations. This study tested a novel, two-punch therapy in mice—Selenium nanoparticles (Se NPs) and platelet-rich plasma (PRP)—to boost healing. First, a mouse model of diabetes was created. Then, Se NPs were crafted for their impressive antioxidant and antimicrobial powers. PRP, packed with growth factors, was extracted from the mice's blood. Wound healing was tracked for 28 days through photos, scoring tools, and tissue analysis. Se NPs alone spurred healing, and PRP added extra fuel. Furthermore, when used in combination with PRP, the healing process was accelerated due to the higher concentration of growth factors in PRP. Notably, the combination of Se NPs and PRP exhibited a synergistic effect, significantly enhancing wound healing in diabetic mice. These findings hold promise for the treatment of diabetic wounds and have the potential to reduce the need for lower limb amputations associated with diabetic foot ulcers. The innovative combination therapy using Se NPs and PRP shows great potential in expediting the healing process and addressing the challenges of impaired wound healing in individuals with diabetes. This exciting finding suggests this therapy could change diabetic wound management, potentially saving limbs and improving lives.

  • Rapid RP-HPLC Detection Method For Quantification Of Gentamicin Sulfate Loaded Wound Dressing Nanofiber Formulation With Accelerated In Vivo Wound Healing
    Mona M. Agwa, Mohamed R. El-Aassar, Rehab I. Moustafa, Hassan Elsayed, and Nagham G. El-Beheri

    Egypts Presidential Specialized Council for Education and Scientific Research

  • Enhancing bioelectricity generation from wastewater in microbial fuel cells using carbon nanomaterials
    Yasser A Attia, Mohamed Samer, Mahmoud SM Mohamed, Mohamed Salah, Elshaimaa Moustafa, R M Abdel Hameed, Hassan Elsayed, and Essam M Abdelsalam

    Wiley
    AbstractBACKGROUNDMicrobial fuel cells (MFCs) offer a promising approach for treating wastewater and generating electrical energy simultaneously. However, their implementation in wastewater treatment plants is hindered by the limited electricity generation, often attributed to the electrolyte's high resistance. This study aimed to improve bioelectricity generation in MFCs by adding nanomaterials to the electrolyte to enhance conductivity.RESULTSThree types of nanomaterials – carbon nanotubes (CNTs), graphitic carbon nitride (g‐C3N4), and reduced graphene oxide (r‐GO) – were synthesized and addition to the electrolyte at a concentration of 50 mg in 1.5 L. MFC performance was evaluated, employed a hydraulic retention time (HRT) of 140 h, and compared to a control with no nanomaterials added. The addition of nanomaterials significantly improved MFC performance. Compared to the control, the MFCs with CNTs, g‐C3N4, and r‐GO exhibited higher voltage: 1.301 V (CNTs), 1.286 V (g‐C3N4), 1.280 V (r‐GO) versus 0.570 V (control); increased power density: 14.11 mW m−3 (CNTs), 13.78 mW m−3 (g‐C3N4), 13.66 mW m−3 (r‐GO) versus 2.71 mW m−3 (control); enhanced areal power density: 21.06 mW m−2 (CNTs), 20.57 mW m−2 (g‐C3N4), 20.39 mW m−2 (r‐GO) versus 4.04 mW m−2 (control); and improved coulombic efficiency: 19.43% (CNTs), 19.19% (g‐C3N4), 19.11% (r‐GO) versus 8.54% (control).CONCLUSIONIncorporating nanomaterials into the MFC electrolyte significantly increased bioelectricity generation by 5.21 times and coulombic efficiency by 2.28 times compared to the control. This improvement is attributed to the high specific surface area of the nanomaterials, which facilitates the adhesion and growth of microorganisms around the anode, enhancing direct electron transfer. © 2024 Society of Chemical Industry (SCI).

  • Photoactivation of nano MgO anchored g-C<inf>3</inf>N<inf>4</inf> enhances biodiesel production in Chlorella sorokiniana: A sustainable approach
    Amany Khalifa, Maryam Faried, Essam M. Abdelsalam, Mohamed Samer, Mohamed A. Moselhy, Hassan Elsayed, and Yasser A. Attia

    Wiley
    AbstractInvestigating the potential of magnesium oxide (MgO), graphitic carbon nitride (g‐C3N4), and their composite nanoparticles as nutrient sources for enhanced microalgae biodiesel production formed the core of this study. Supplementing the growth medium with g‐C3N4 and MgO/g‐C3N4 nanoparticles significantly increased microalgae (Chlorella sorokiniana) growth and lipid accumulation, culminating in a 58 mg/L lipid concentration. Interestingly, while MgO nanoparticles alone led to the highest biodiesel yield, the synergistic effect of MgO and g‐C3N4 in the composite nanoparticles improved nutrient availability and facilitated optimal microalgae growth and lipid accumulation. These findings pave the way for further research and development of nanoparticle‐based strategies to optimize microalgae‐based biodiesel production, offering a promising avenue for a more sustainable and efficient future of biofuel generation. The results showed that the addition of 15 mg/L of MgO NPs produced the maximum biodiesel yield which reached 61.5 mg/L.

  • Modulation of immune responses to liposomal vaccines by intrastructural help
    Dominik Damm, Ehsan Suleiman, Jannik T. Wagner, Stephan Klessing, Felix Pfister, Hassan Elsayed, Bernd Walkenfort, Jule Stobrawe, Julia Mayer, Elisabeth Lehner,et al.

    Elsevier BV

  • Harnessing immunoinformatics for developing a multiple-epitope peptide-based vaccination approach against SARS-CoV-2 spike protein
    Rehab I. Moustafa, Ahmed H.I. Faraag, Reem El-Shenawy, Mona M. Agwa, and Hassan Elsayed

    Elsevier BV

  • Carbohydrate ligands-directed active tumor targeting of combinatorial chemotherapy/phototherapy-based nanomedicine: A review
    Mona M. Agwa, Heba Elmotasem, Hassan Elsayed, Abdallah S. Abdelsattar, Ahmed M. Omer, Doaa T. Gebreel, Mohamed S. Mohy-Eldin, and Moustafa M.G. Fouda

    Elsevier BV

  • EMPIRICAL MODELING FOR REPORTED CASES AND DEATHS OF COVID-19 IN EGYPT DURING THE ACCELERATED SPREAD AND PREDICTION OF THE DELAYED PHASE
    Essam Kotb, Noureddine Ouerfelli, Naouel Zrelli, Khaoula Briki, Asmaa Ahmed, Mohammed Medhat, Hassan Elsayed, and Mohamed El-Kassas

    Egypts Presidential Specialized Council for Education and Scientific Research

  • Impact of photoactivated cobalt oxide nanoparticles addition on manure and whey for biogas production through dry anaerobic co-digestion
    M. Samer, E. M. Abdelsalam, S. Mohamed, H. Elsayed, and Y. Attia

    Springer Science and Business Media LLC

  • Vitamin D3/phospholipid complex decorated caseinate nanomicelles for targeted delivery of synergistic combination therapy in breast cancer
    Mona M. Agwa, Marwa M. Abu-Serie, Doaa A. Abdelmonsif, Nermine Moussa, Hassan Elsayed, Sherine N. Khattab, and Sally Sabra

    Elsevier BV

  • Carbon nanotubes catalyzed UV-trigger production of hyaluronic acid from Streptococcus equi
    Yasser A. Attia, Ashwaq M. Al Nazawi, Hassan Elsayed, and Mahmoud W. Sadik

    Elsevier BV

  • Clinical features and laboratory characteristics of patients hospitalized with COVID-19: Single centre report from Egypt
    Mohamed El Kassas, Noha Asem, Amr Abdelazeem, Ahmad Madkour, Hamdy Sayed, Ahmed Tawheed, Ahmed Al Shafie, Mahmoud Gamal, Hassan Elsayed, Mohamed Badr,et al.

    Journal of Infection in Developing Countries
    Introduction: The recently discovered novel coronavirus disease (COVID-19) has emerged in Wuhan, China, since January 2020. Egypt reported a low incidence of infection when compared with other countries. The aim of the study was to assess the characterization of COVID-19 infection among the Egyptian population.&#x0D; Methodology: Data were collected from a single COVID-19 quarantine hospital in Cairo. A total number of 195 cases were included with their clinical, laboratory, and radiological data.&#x0D; Results: Three different age groups behaved differently for COVD-19 infection. The pediatric age group was asymptomatic entirely, the middle age group (18-50 years) were asymptomatic in 53.3% of cases, while 77.9% of those above 50 years were symptomatic (p ≤ 0.001). The latter group had a high incidence of COVID-pneumonia in (83.1%), and moderate to critical presentations were encountered in 66.3% of them. Neutrophil to lymphocyte (N/L) ratio correlated directly with the age and case severity. C-reactive protein (CRP) and computed tomography scan chest (CT-chest) had added value on COVID-19 diagnosis in suspected cases.&#x0D; Conclusions: In Egypt, patients above 50 years are at a higher risk for symptomatic COVID-19 infection and leaner for moderate to critical COVID-19 presentation. The triad of CT-chest, CRP, and N/L ratio could be an integrated panel for assessing disease severity.

  • Intrastructural help: Harnessing T helper cells induced by licensed vaccines for improvement of HIV env antibody responses to virus-like particle vaccines
    Hassan Elsayed, Ghulam Nabi, William J. McKinstry, Keith K. Khoo, Johnson Mak, Andres M. Salazar, Matthias Tenbusch, Vladimir Temchura, and Klaus Überla

    American Society for Microbiology
    ABSTRACT Induction of persistent antibody responses by vaccination is generally thought to depend on efficient help by T follicular helper cells. Since the T helper cell response to HIV Env may not be optimal, we explored the possibility of improving the HIV Env antibody response to virus-like particle (VLP) vaccines by recruiting T helper cells induced by commonly used licensed vaccines to provide help for Env-specific B cells. B cells specific for the surface protein of a VLP can internalize the entire VLP and thus present peptides derived from the surface and core proteins on their major histocompatibility complex class II (MHC-II) molecules. This allows T helper cells specific for the core protein to provide intrastructural help for B cells recognizing the surface protein. Consistently, priming mice with an adjuvanted Gag protein vaccine enhanced the HIV Env antibody response to subsequent booster immunizations with HIV VLPs. To harness T helper cells induced by the licensed Tetanolpur vaccines, HIV VLPs that contained T helper cell epitopes of tetanus toxoid were generated. Tetanol-immunized mice raised stronger antibody responses to immunizations with VLPs containing tetanus toxoid T helper cell epitopes but not to VLPs lacking these epitopes. Depending on the priming immunization, the IgG subtype response to HIV Env after the VLP immunization could also be modified. Thus, harnessing T helper cells induced by other vaccines appears to be a promising approach to improve the HIV Env antibody response to VLP vaccines. IMPORTANCE Induction of HIV Env antibodies at sufficient levels with optimal Fc effector functions for durable protection remains a challenge. Efficient T cell help may be essential to induce such a desirable antibody response. Here, we provide proof of concept that T helper cells induced by a licensed vaccine can be harnessed to provide help for HIV Env-specific B cells and to modulate the Env-specific IgG subtype response.

  • Mouse monoclonal antibody towards E1 specific epitope blocks viral entry and intracellular viral replication in vitro
    Ashraf A. Tabll, Rehab I. Moustafa, Yasmine S. El Abd, Noha G. Bader El Din, Reem El-Shenawy, Hassan Yousef, Manal Hussein, Reham M. Dawood, Moataza H. Omran, and Mostafa K. El-Awady

    Informa UK Limited
    We characterized viral neutralization by a murine monoclonal antibody (mAb315) developed against conserved E1 specific epitope aa 315–323 at pre- and post-binding steps of infection into Huh7 cells. Detection of native virus in infected Huh7 cells by mAb315 were demonstrated by immunostaining. Inhibitions of viral entry by three different concentrations of mAb315 were measured by intracellular amplification of HCV RNA post infection. HCV RNA positive sera from 24 patients were used to infect Huh7 cell line in absence or presence of mouse monoclonal antibody produced in Balb/c mice or culture supernatant of mouse hybrid cells. Monoclonal Ab mAb315 could detect synthetic peptide p315 adsorbed on peripheral human lymphocytes by flow cytometry and showed high immuno reactivity to E1 viral antigen in infected Huh7 cells by immunostaining. Antibody-mediated neutralization assays demonstrated the ability of mAb315 to block HCV binding/entry to target cells at 0.73 mg/mL ascitic fluid or 250 µg/mL culture supernatant of mouse hybrid cells. Sixteen of 24 infected sera could infect Huh7 cells (67%). Binding/entry of HCV was completely blocked by mAb315 in 11/16 cases (69%). These findings suggest that mAb315 can induce HCV neutralization in vitro, which makes it a candidate for developing HCV therapeutic antibodies

  • Murine neutralizing antibody response and toxicity to synthetic peptides derived from E1 and E2 proteins of hepatitis C virus
    Mostafa K. El-Awady, Ashraf A. Tabll, Hassan Yousif, Yasmin El-Abd, Mohamed Reda, Samy B. Khalil, Abdel Rahman El-Zayadi, Maysa H. Shaker, and Noha G. Bader El Din

    Elsevier BV

  • Conserved peptides within the E2 region of Hepatitis C virus induce humoral and cellular responses in goats
    Mostafa K El-Awady, Ashraf A Tabll, Yasmine S El-Abd, Hassan Yousif, Mohsen Hegab, Mohamed Reda, Reem El Shenawy, Rehab I Moustafa, Nabila Degheidy, and Noha El Din

    Springer Science and Business Media LLC