Kateryna Pantiukh
@ut.ee
University of Tartu
Institute of Genomics, University of Tartu
EDUCATION
2009 – 2011 - Lomonosov Moscow State University (MSU) - Master's degree, Biotechnology
2009 – 2011 - Taras Shevchenko National University of Kyiv - Master's degree, Cytology, and histology
2005 – 2009 - Taras Shevchenko National University of Kyiv – Bachelor’s degree, Biology
RESEARCH INTERESTS
microbial genomics and metagenomics
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
K. S. Pantiukh, I. V. Rukin, S. M. Portnov, A. Khatib, S. L. Panteleev, and A. M. Mazur
Institute of Cytology and Genetics, SB RAS
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K. S. Pantiukh, I. V. Rukin, S. V. Portnov, A. Khatib, S. V. Panteleev, and A. M. Mazur
Institute of Cytology and Genetics, SB RAS
The integration of high technologies into livestock production has been actively occurring in the last decade in the countries with a developed animal breeding. First of all, we are talking about reproductive technologies (IVF) and genomic technologies (general genomic evaluation of animal and genomic evaluation of breeding value). Combining reproductive and genomic technologies is a promising approach that allows receiving highquality breeding cattle in the shortest possible time. The basis of the proposed technology for accelerated reproduction of high-value breeding cattle is to obtain information about the genome of the embryo for genomic evaluation. The amount of genetic material that can be obtained for research is extremely limited, as it is necessary to preserve the viability of the embryo. The stage of the whole genome amplification was introduced to obtain a high quality of genetic material in a sufficient quantity. The main purpose of this work is to assess the possibility of using embryo biopsy specimens (bsp) for embryo genotyping using microarray chips and predicting the carrier status of lethal haplotypes at the embryo stage. We obtained 100 cattle embryos, of which 78 biopsy specimens were taken to analysis. For the biopsies obtained we performed the whole genome amplification. The quality and quantity of DNA for all the 78 samples after the whole genome amplification were satisfactory for further genotyping. The quality of the performed genotyping was satisfactory and allowed the assessment of lethal haplotype carriers (determining the sex of the animal and identification of the carrier status for sevenHolsteinlethal haplotypes). We tested 78 embryos. From the genotyping analysis, there was detected one carrier status for three lethal haplotypes, HH0 (Brachyspina), HH5, and HCD. The carrier status of HH0 and HH5 was confirmed by testing the casual mutation using PCR analysis. The carrier status for HCD has not been confirmed by casual mutation analysis. The situation in which an animal is an HCD carrier, but not the carrier of a casual mutation, can be explained. The putative ancestor of the haplotype is the bull HOCAN000000334489 WILLOWHOLME MARK ANTHONY (year of birth is 1975), but a casual mutation associated with this disease has arisen only in his descendant HOCAN000005457798 MAUGHLIN STORM (year of birth is 1991). The results obtained confirm the importance of testing the casual mutation in the animals that are carriers of lethal haplotypes according to the genotyping data.
A.V. Brigida, , V.I. Sorokin, S.N. Kovalchuk, K.S. Pantiukh, I.V. Rukin, K.A. Rozhin, , , ,et al.
Sel'skokhozyaistvennaya Biologiya Editorial Office (SBEO), NPO
The main problem restricting the wide use of reproductive biotechnology in animal husbandry is insufficiently developed methods for selection of donor cows for embryo transfer. The objective reason is the variability of the ovarian response to gonadotropins injections. Until now, there is no reliable information about possibility of forecasting the embryo productivity of donor cows before gonadotropin stimulation, which affects substantively the economic feasibility of embryo transfer as a method of accelerated cattle reproduction. We have applied the post-pressing analysis of ovaries echograms for forecasting the embryo productivity of donor cows on the basis of comparisons of quantitative and qualitative indicators of ovaries after induced superovulation and its’ echographic characteristics. We carried out morphofunctional study of ovaries in donor cows (n = 30) on day 10 of estrous cycle, before artificial insemination (estrus) and on day 7 of the inducted estrous cycle, immediately before the extraction of embryos using data on post-pressing ovarian morphometry. Animals were divided into three groups (I, II и III, n = 10 for each group) with yellow body length of 2.5 cm, 1.5-2.5 cm and 1.5 cm, respectively. Echographic visualization of the ovaries was performed using endorectal ultrasonography. Polyovulatory response of ovaries was induced with FSHsuper (Russia) injected eight times, with 12 h interval, at decreasing doses. The embryos were recovered on day 7 after artificial insemination. Optimal criteria for predicting the polyovulatory response of ovaries and the quantity of embryos were determined on the basis of the ovarian morphometry. Statistically significant differences with the control were assessed by the Student’s t-test. It was found that the average areas of the ovaries on the echograms were 7.9±0.94, 5.7±0.78 and 3.5±0.06 cm2 for group I, group II and group III, respectively. The area of the yellow body in group I averaged 4.5±1.21 cm2, was 2.08 cm2 higher (P 0.05) than in group II, and exceeded the corresponding parameter in group III by 3.43 cm2 (P 0.05). A comparative evaluation of the ratio of the yellow body areas to the ovaries area of each animal and on average along the groups showed that in group I with the ratio of 57.1±3.01 % the number of yellow bodies was 11.6±1.26 and the average yield of the embryos was 9.3±1.23 per animal. In group II with the ratio of the areas of yellow bodies and ovaries of 42.1±2.9 % the number of yellow bodies before embryos recovery was 5.7±1.24, and 4.6±1.01 embryos were recovered per procedure. The lowest embryo recovery (less than one embryo per procedure) was observed in group III with the relative area of yellow bodies of 30.2±2.56 % and the average number of the yellow bodies of 1.8±0.18. Comparison of the size of the yellow bodies before the induction of polyovulation and data characterizing the efficiency of induction of superovulation and recovery of embryos showed that in animals with a ratio of the areas of the yellow body and ovary more than 50 %, high response can be obtained resulting in 11.6±1.26 yellow bodies and 9.3±1.23 embryos per extraction.
O. A. Shulga, A. V. Nedoluzhko, A. V. Shchennikova, N. M. Gruzdeva, A. A. Shelenkov, F. S. Sharko, A. S. Sokolov, E. S. Pantiukh, S. M. Rastorguev, E. B. Prokhortchouk,et al.
Springer Science and Business Media LLC
Kateryna S. Pantiukh, Nikolay N. Chekanov, Igor V. Zaigrin, Alexei M. Zotov, Alexander M. Mazur, and Egor B. Prokhortchouk
F1000 Research Ltd
Concerns of traditional prenatal aneuploidy testing methods, such as low accuracy of noninvasive and health risks associated with invasive procedures, were overcome with the introduction of novel noninvasive methods based on genetics (NIPT). These were rapidly adopted into clinical practice in many countries after a series of successful trials of various independent submethods. Here we present results of own NIPT trial carried out in Moscow, Russia. 1012 samples were subjected to the method aimed at measuring chromosome coverage by massive parallel sequencing. Two alternative approaches are ascertained: one based on maternal/fetal differential methylation and another based on allelic difference. While the former failed to provide stable results, the latter was found to be promising and worthy of conducting a large-scale trial. One critical point in any NIPT approach is the determination of fetal cell-free DNA fraction, which dictates the reliability of obtained results for a given sample. We show that two different chromosome Y representation measures—by real-time PCR and by whole-genome massive parallel sequencing—are practically interchangeable (r=0.94). We also propose a novel method based on maternal/fetal allelic difference which is applicable in pregnancies with fetuses of either sex. Even in its pilot form it correlates well with chromosome Y coverage estimates (r=0.74) and can be further improved by increasing the number of polymorphisms.
Sukhikh G.T. Sukhikh, Trofimov D.Yu. Trofimov, Barkov I.Yu. Barkov, Donnikov A.E. Donnikov, Shubina E.S. Shubina, Korostin D.O. Korostin, Ekimov A.N. Ekimov, Goltsov A.Yu. Goltsov, Bakharev V.A. Bakharev, Karetnikova N.A. Karetnikova,et al.
Bionika Media
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
Publications
Pantiukh KS, Chekanov NN, Zaigrin IV, Zotov AM, Mazur AM, Prokhortchouk EB. Report on noninvasive prenatal testing: classical and alternative approaches. F1000Res. 2016 Apr 22;5:722.
Pantiukh K., Shanko A., Khrameeva E., Mospan N. Development a new method for noninvasive prenatal detection of fetal aneuploidy// Prenat Diagn 2013; 33(Suppl. 1): P-53.
Patent 2585519 (27.05.2016) A method for selecting target DNA regions