Mohsin Shad

@pu.edu.pk

PhD & Post Doctorate (Biochemistry & Molecular Biology)
School of Biological Sciences, University of The Punjab, Lahore, Pakistan

Mohsin Shad
To achieve a challenging position in a prestigious institute/ research center, where I may
dedicate my versatile abilities for science and technology.

EDUCATION

Dr. Mohsin Shad is a distinguished Biochemist and Molecular Biologist with over 8 years of experience in research and academia. He holds a Ph.D. and Postdoctoral training in Biochemistry and Molecular Biology. He specializes in recombinant enzyme and protein engineering for industrial and therapeutic applications. Dr Shad has held prestigious roles, including Postdoctoral Research Associate at Avlon Industries (USA), Visiting Scientist at the Rosalind Franklin Institute, University of Oxford (UK), and Instructor of Biochemistry at the Virtual University of Pakistan. His academic affiliations span several leading Pakistani institutions such as SBS, VU, CEMB, FCCU, and UVAS. Dr Shad has authored multiple high-impact publications focusing on the structural and functional engineering of microbial and archaeal enzymes, including α-amylases and lysophospholipases, for industrial applications.

RESEARCH, TEACHING, or OTHER INTERESTS

Biochemistry, Genetics and Molecular Biology, Biochemistry, Molecular Biology, Structural Biology

FUTURE PROJECTS

Engineering Recombinant Peptides and Enzymes for Industrial and Therapeutic Applications

The proposed project aims to harness advanced protein engineering strategies for the design, production, and application of recombinant peptides and enzymes with high industrial and therapeutic potential. Building upon previous research in keratin-associated peptides for cosmetic biotechnology and thermostable α-amylases for industrial biocatalysis, this project will focus on developing novel bioactive peptides with applications in hair and skin health, alongside engineered enzymes with enhanced thermostability, catalytic efficiency, and substrate specificity for the textile, detergent, starch, and food industries. A combination of computational biology (in silico modeling, molecular docking, and dynamics simulations) and experimental approaches (site-directed mutagenesis, X-ray crystallography, ITC, and SUMO-fusion technology) will be employed to achieve targeted modifications. Furthermore, potential therapeutic peptide leads will be explored for antimicrobial and immunomodulatory.


Applications Invited
307

Scholar Citations

10

Scholar h-index

11

Scholar i10-index

RECENT SCHOLAR PUBLICATIONS

  • Fluoride Beyond Enamel: Impacts of Topical and Systemic Fluoride on the Oral and Gut Microbiome, A Review
    M SHAD, M ASLAM, AM ALMUQRIN, MFU REHMAN, MFUL HAQUE
    Fluoride 405 , 2025
    2025
  • Exploration of Comprehensive Structural and Functional Potential of Recombinant Proteins Using Cutting-Edge Bioinformatics Tools
    M Shad, A Liaqat, A Nazir, N Hussain, K Yaqoob, MW Akhtar, M Sajjad
    Applied Biochemistry and Biotechnology 197 (11), 7015-7043 , 2025
    2025
    Citations: 1
  • Production of an Asparaginase-Like Domain of AnsA from Methanocaldococcus jannaschii in Escherichia coli, its purification and In Silico Analysis
    M Ijaz, M Shad, A Nazir, N Azim, M Sajjad
    Pakistan Journal of Zoology 57 (4), 1889 , 2025
    2025
    Citations: 2
  • Investigating the structural and functional snapshots of Bacillus licheniformis alpha-amylase through protein engineering strategies
    M Shad, MW Akhtar, M Sajjad
    International Journal of Biological Macromolecules 307, 142243 , 2025
    2025
    Citations: 2
  • Immunological assessment of NSFu1: A novel fusion molecule constructed from structural proteins of SARS-CoV-2 for improving COVID-19 antibody detection
    S Arif, M Akhter, A Anwar, S Javaid, Z Ashi, M Shad, A Rahman, H Abbas, ...
    Archives of Microbiology 207 (4), 88 , 2025
    2025
    Citations: 2
  • Impact of silica nanoforms on the health and yield of crops
    M Usman, M Atiq, NA Rajput, M Shad, AM Arif
    Nanomaterials for enhanced plant-based food production, 111-124 , 2025
    2025
    Citations: 1
  • Application of SUMO fusion technology for the enhancement of stability and activity of lysophospholipase from Pyrococcus abyssi
    A Nazir, M Shad, HM Rehman, N Azim, M Sajjad
    World Journal of Microbiology and Biotechnology 40 (6), 183 , 2024
    2024
    Citations: 10
  • Investigating the effect of SUMO fusion on solubility and stability of amylase-catalytic domain from Pyrococcus abyssi
    M Shad, A Nazir, M Usman, MW Akhtar, M Sajjad
    International Journal of Biological Macromolecules 266, 131310 , 2024
    2024
    Citations: 22
  • Structural and functional insights of starch processing α-amylase from hyperthermophilic archaeon Pyrococcus abyssi
    M Shad, HM Rehman, MW Akhtar, M Sajjad
    Carbohydrate Research 539, 109122 , 2024
    2024
    Citations: 8
  • Recombinant production and characterization of a metal ion-independent Lysophospholipase from a hyperthermophilic archaeon Pyrococcus abyssi DSM25543
    A Nazir, M Shad, N Rashid, N Azim, M Sajjad
    International Journal of Biological Macromolecules 259, 129345 , 2024
    2024
    Citations: 7
  • Efficacy of Green Synthesized Silver Based Nanomaterials Against Early Blight of Tomato Caused by Alternaria solani
    M Usman, M Atiq, NA Rajput, ST Sahi, M Shad, N Lili, S Iqbal, AM Arif, ...
    Journal of Crop Health 76 (1), 105-115 , 2024
    2024
    Citations: 39
  • Structural engineering and truncation of α-amylase from the hyperthermophilic archaeon Methanocaldococcus jannaschii
    M Shad, M Sajjad, QA Gardner, S Ahmad, MW Akhtar
    International Journal of Biological Macromolecules 256, 128387 , 2024
    2024
    Citations: 9
  • Exploration of computational approaches to predict the structural features and recent trends in α‐amylase production for industrial applications
    M Shad, N Hussain, M Usman, MW Akhtar, M Sajjad
    Biotechnology and Bioengineering 120 (8), 2092-2116 , 2023
    2023
    Citations: 45
  • Structural-Functional Characterization of Cytochrome b in bc1 and b6 f Complexes along with Polymorphic Analysis
    M Shad, M Usman, QA Gardner
    Pakistan Journal of Zoology 55 (2), 975-986 , 2023
    2023
    Citations: 17
  • Overexpression of the AGL42 gene in cotton delayed leaf senescence through downregulation of NAC transcription factors
    A Latif, S Azam, N Shahid, MR Javed, Z Haider, A Yasmeen, S Sadaqat, ...
    Scientific Reports 12 (1), 21093 , 2022
    2022
    Citations: 11
  • The Rv3874-Rv3875 chimeric protein shows a promiscuous serodiagnostic potential for tuberculosis
    N Mahmood, M Akhter, N Hussain, M Shad, Z un Nisa, IH Khan, ...
    Tuberculosis 136, 102253 , 2022
    2022
    Citations: 11
  • Enhancing the resilience of transgenic cotton for insect resistance
    M Shad, A Yasmeen, S Azam, A Bakhsh, A Latif, N Shahid, S Sadaqat, ...
    Molecular Biology Reports 49 (6), 5315-5323 , 2022
    2022
    Citations: 19
  • Mistranslation of Genetic Codon and Their Controlling Mechanism in Cell
    Mohsin Shad1*, Ayesha Malik2 , Momina Javid2 , Salah ud Din2 , Sana Shakoor2 ...
    Biochemistry and Molecular Biology Journal 8 (1), 51 , 2022
    2022
  • Recombinant production and validation of immunogenic fragment from RBD of SARS-CoV-2 spike protein
    S Javaid, Z Ashi, A Anwar, M Akhter, S Arif, M Shad, MW Akhtar, M Sajjad
    Pakistan Journal of Biochemistry and Molecular Biology 55 (2), 138-145 , 2022
    2022
    Citations: 5
  • Potential of an Epitope-rich Peptide from S2 Domain of Spike Protein in Serodiagnosis of SARS-CoV-2
    Z Ashi, S Javaid, S Arif, M Akhter, A Anwar, M Shad, M Sajjad, MW Akhtar
    Pak. J. Biochem. Mol. Biol 55 (3), 138-145 , 2022
    2022
    Citations: 5

MOST CITED SCHOLAR PUBLICATIONS

  • Exploration of computational approaches to predict the structural features and recent trends in α‐amylase production for industrial applications
    M Shad, N Hussain, M Usman, MW Akhtar, M Sajjad
    Biotechnology and Bioengineering 120 (8), 2092-2116 , 2023
    2023
    Citations: 45
  • Efficacy of Green Synthesized Silver Based Nanomaterials Against Early Blight of Tomato Caused by Alternaria solani
    M Usman, M Atiq, NA Rajput, ST Sahi, M Shad, N Lili, S Iqbal, AM Arif, ...
    Journal of Crop Health 76 (1), 105-115 , 2024
    2024
    Citations: 39
  • Development of broad-spectrum and sustainable resistance in cotton against major insects through the combination of Bt and plant lectin genes
    S Din, S Azam, AQ Rao, M Shad, M Ahmed, A Gul, A Latif, MA Ali, ...
    Plant Cell Reports 40 (4), 707-721 , 2021
    2021
    Citations: 38
  • Transformation and evaluation of Broad-Spectrum insect and weedicide resistant genes in Gossypium arboreum (Desi Cotton
    AAS Muhammad Sufyan Tahir, Ayesha Latif, Samina Bashira, Mohsin Shad ...
    GM CROPS & FOOD 12 (1), 292-302 , 2021
    2021
    Citations: 23
  • Investigating the effect of SUMO fusion on solubility and stability of amylase-catalytic domain from Pyrococcus abyssi
    M Shad, A Nazir, M Usman, MW Akhtar, M Sajjad
    International Journal of Biological Macromolecules 266, 131310 , 2024
    2024
    Citations: 22
  • Enhancing the resilience of transgenic cotton for insect resistance
    M Shad, A Yasmeen, S Azam, A Bakhsh, A Latif, N Shahid, S Sadaqat, ...
    Molecular Biology Reports 49 (6), 5315-5323 , 2022
    2022
    Citations: 19
  • Structural-Functional Characterization of Cytochrome b in bc1 and b6 f Complexes along with Polymorphic Analysis
    M Shad, M Usman, QA Gardner
    Pakistan Journal of Zoology 55 (2), 975-986 , 2023
    2023
    Citations: 17
  • Comparative analysis of Constitutive and fiber-specific promoters under the expression pattern of Expansin gene in transgenic Cotton
    A Yaqoob, A Ali Shahid, IB Salisu, S Shakoor, M Usmaan, M Shad, ...
    Plos one 15 (3), e0230519 , 2020
    2020
    Citations: 16
  • Overexpression of the AGL42 gene in cotton delayed leaf senescence through downregulation of NAC transcription factors
    A Latif, S Azam, N Shahid, MR Javed, Z Haider, A Yasmeen, S Sadaqat, ...
    Scientific Reports 12 (1), 21093 , 2022
    2022
    Citations: 11
  • The Rv3874-Rv3875 chimeric protein shows a promiscuous serodiagnostic potential for tuberculosis
    N Mahmood, M Akhter, N Hussain, M Shad, Z un Nisa, IH Khan, ...
    Tuberculosis 136, 102253 , 2022
    2022
    Citations: 11
  • Application of SUMO fusion technology for the enhancement of stability and activity of lysophospholipase from Pyrococcus abyssi
    A Nazir, M Shad, HM Rehman, N Azim, M Sajjad
    World Journal of Microbiology and Biotechnology 40 (6), 183 , 2024
    2024
    Citations: 10
  • Structural engineering and truncation of α-amylase from the hyperthermophilic archaeon Methanocaldococcus jannaschii
    M Shad, M Sajjad, QA Gardner, S Ahmad, MW Akhtar
    International Journal of Biological Macromolecules 256, 128387 , 2024
    2024
    Citations: 9
  • Structural and functional insights of starch processing α-amylase from hyperthermophilic archaeon Pyrococcus abyssi
    M Shad, HM Rehman, MW Akhtar, M Sajjad
    Carbohydrate Research 539, 109122 , 2024
    2024
    Citations: 8
  • Recombinant production and characterization of a metal ion-independent Lysophospholipase from a hyperthermophilic archaeon Pyrococcus abyssi DSM25543
    A Nazir, M Shad, N Rashid, N Azim, M Sajjad
    International Journal of Biological Macromolecules 259, 129345 , 2024
    2024
    Citations: 7
  • Recombinant production of Mtb antigens and their purification by affinity chromatography
    S Arif, M Akhtar, M Shad, MW Akhtar
    Pakistan Journal of Biochemistry and Molecular Biology 55 (4), 226-231 , 2022
    2022
    Citations: 7
  • Recombinant production and validation of immunogenic fragment from RBD of SARS-CoV-2 spike protein
    S Javaid, Z Ashi, A Anwar, M Akhter, S Arif, M Shad, MW Akhtar, M Sajjad
    Pakistan Journal of Biochemistry and Molecular Biology 55 (2), 138-145 , 2022
    2022
    Citations: 5
  • Potential of an Epitope-rich Peptide from S2 Domain of Spike Protein in Serodiagnosis of SARS-CoV-2
    Z Ashi, S Javaid, S Arif, M Akhter, A Anwar, M Shad, M Sajjad, MW Akhtar
    Pak. J. Biochem. Mol. Biol 55 (3), 138-145 , 2022
    2022
    Citations: 5
  • Evaluation of Immunogenic Response of Recombinant Nucleocapsid Protein of SARS-CoV-2
    A Anwar, MZ Younas, M Shad, M Akhtar, S Arif, A Rahman, M Sajjad, ...
    PAKISTAN JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY 55 (4), 242-251 , 2022
    2022
    Citations: 4
  • Production of an Asparaginase-Like Domain of AnsA from Methanocaldococcus jannaschii in Escherichia coli, its purification and In Silico Analysis
    M Ijaz, M Shad, A Nazir, N Azim, M Sajjad
    Pakistan Journal of Zoology 57 (4), 1889 , 2025
    2025
    Citations: 2
  • Investigating the structural and functional snapshots of Bacillus licheniformis alpha-amylase through protein engineering strategies
    M Shad, MW Akhtar, M Sajjad
    International Journal of Biological Macromolecules 307, 142243 , 2025
    2025
    Citations: 2

Publications

Total Publications: 27 (Scopus / WoS indexed and Google Scholar)

Book Chapter: 1 (Elsevier – Nanomaterials for Enhanced Plant-Based Food Production, 2025)

Patent (Under Review): Novel Fusion Protein NSFU1 for Enhanced Sensitivity in COVID-19 Serodiagnostic Tests (Application No. 438/2024)

Citations (Google Scholar): 210 | H-Index: 8

Cumulative Impact Factor: ~75

Newsletter Articles: 2

Publications Under Review: 2

Editorial/Reviewer Roles:

Guest Editor – BMC Methods (Protein Engineering Issue)

Reviewer – The Protein Journal (Springer Nature)

Conference Contributions:

6 Oral Presentations (National & International)

16 Poster Presentations

GRANT DETAILS

Grant Details

Postdoctoral Fellowship Grant (2024–2025)
School of Biological Sciences, University of the Punjab, Lahore

Funded by Avlon Industries Inc., USA

Research Project: Designing and Production of Keratin-Associated Peptides of Human Origin and Their Application for Improving Hair Health

HEC-IRSIP Fellowship Grant (2023–2024)
The Rosalind Franklin Institute, University of Oxford, UK

Funded by Higher Education Commission (HEC), Pakistan

Research Project: Structural and Functional Insights of Purified Enzyme through X-ray Crystallography, Site-Directed Mutagenesis, and Isothermal Titration Calorimetry (ITC)

SBS-PhD Fellowship Grant (2019–2024)
School of Biological Sciences, University of the Punjab, Lahore

Funded by School of Biological Sciences (SBS), PU Lahore

Research Project: Development of Highly Thermostable Alpha-Amylase through Protein Engineering for Industrial Applications

NRPU Project Involvement (2018–2019)
Centre of Excellence in Molecular Biology (CEMB), University of the Punjab

Worked as a Research Assistant on the HEC-NRPU Project (#8243)

Project Focus: Next-generation Bt and Plant Lectin Genes for Insect Resistance in Cotton

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)

Patent (Filed / Under Review):
Novel Fusion Protein NSFU1 for Enhanced Sensitivity in COVID-19 Serodiagnostic Tests
Patent Application No. 438/2024 | Filing Date: June 14, 2024 | Applicant: School of Biological Sciences, University of the Punjab, Lahore

Product Development:

Designed and produced Keratin-Associated Peptides of Human Origin (Postdoctoral Research, funded by Avlon Industries Inc., USA) for potential application in hair health and cosmetic industry.

Engineered highly thermostable α-amylase variants through protein engineering for potential industrial biocatalysis applications (PhD research).

Editorial/Scientific Outputs:

Guest Editor for BMC Methods (Protein Engineering Issue).

Reviewer for The Protein Journal (Springer Nature).

CONSULTANCY

Avlon Industries Inc., USA – Provided consultancy on the design and production of keratin-associated peptides for application in hair health and cosmetic biotechnology (2024–2025).

Agricultural Biotechnology Projects (CEMB, University of the Punjab, Lahore) – Consulted on the development of insect-resistant cotton using Bt and plant lectin gene constructs (2018–2019).

Enzyme Engineering and Industrial Biocatalysis – Advised academic and industrial teams on protein engineering strategies for improving thermostability and catalytic efficiency of α-amylase for industrial applications.

Scientific Publishing & Peer Review – Served as Guest Editor (BMC Methods, Protein Engineering Issue) and Reviewer (The Protein Journal, Springer Nature), providing expert consultancy in biochemistry, molecular biology, and protein engineering.

Industry, Institute, or Organisation Collaboration

Industry, Institute, Organisation Collaboration

Avlon Industries Inc., USA – Postdoctoral collaboration on the design and production of Keratin-Associated Peptides of Human Origin for hair health applications (2024–2025).

The Rosalind Franklin Institute, University of Oxford, UK – HEC-IRSIP funded collaboration on enzyme production, purification, X-ray crystallography, and protein engineering (2023–2024).

School of Biological Sciences (SBS), University of the Punjab, Lahore, Pakistan – Long-term collaboration on protein engineering, recombinant enzyme development, and biocatalysis research.

Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, Pakistan – Research collaboration on next-generation Bt and plant lectin genes for insect resistance.

University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan – Academic and research collaboration in molecular biology, biotechnology, and plant transformation studies.

National & International Conferences/Workshops – Active collaboration in organizing committees, training workshops, and scientific networking platforms.

INDUSTRY EXPERIENCE

Industry Experience

Postdoctoral Scientist (Industry-Funded Project)
School of Biological Sciences, University of the Punjab, Lahore
Funded by Avlon Industries Inc., USA (Oct 2024 – Jun 2025)

Designed and produced Keratin-Associated Peptides of Human Origin for applications in hair health and cosmetic formulations.

Conducted protein engineering and recombinant peptide production to develop industry-relevant bioproducts.

Bridged academic research with industrial applications, ensuring translational outcomes for cosmetic biotechnology.

Applied Research Contributions

Engineered thermostable α-amylase variants through protein engineering for potential industrial biocatalysis.

Worked on Bt and plant lectin gene constructs for enhanced cotton resistance against insect pests, contributing towards agricultural biotechnology innovations.

STARTUP

Cosmetic Biotechnology Innovation (In Collaboration with Avlon Industries Inc., USA)

Developed keratin-associated peptides of human origin with potential application in hair health products.

Technology holds promise for commercialization in cosmetic formulations, contributing to the nutricosmetics and personal care market.

Industrial Enzyme Engineering

Research on thermostable α-amylase variants provides a foundation for enzyme-based startups targeting starch, textile, detergent, and food industries.

Potential for licensing and technology transfer of engineered enzymes to industrial partners.

Agro-Biotech Applications

Contributions in Bt and plant lectin gene constructs for insect-resistant cotton suggest avenues for agricultural biotechnology startups focusing on sustainable pest management.

Future Plans

Exploring opportunities for spin-offs and incubation projects in protein engineering, recombinant peptides, and enzyme biocatalysis.

Aim to translate academic research into market-ready bioproducts with measurable economic and social impact.

SOCIAL, ECONOMIC, or ACADEMIC BENEFITS

Health & Cosmetic Sector: Development of keratin-associated peptides contributes to innovative hair health solutions, supporting the cosmetic biotechnology industry and improving consumer well-being.

Industrial Biotechnology: Engineering of thermostable α-amylase variants enhances industrial enzyme applications (e.g., textile, starch, food, detergent industries), reducing costs and improving process efficiency.

Agricultural Sustainability: Research on Bt and plant lectin genes provides environmentally friendly pest-resistant cotton varieties, decreasing pesticide use and supporting sustainable agriculture.

Economic Impact: Industry-funded research (Avlon Industries Inc., USA) demonstrates successful academia–industry partnerships, contributing to knowledge transfer and economic growth through applied biotechnology.

Capacity Building: Active involvement in national/international conferences, workshops, and training programs has enhanced scientific knowledge exchange and skill development in protein engineering, molecular biology, and structural biology.

Scientific Advancement: Publications in high-impact journals, a book chapter (Elsevier), and patent development provide significant academic contributions to the fields of biochemistry, molecular biology, and biotechnology.