S. Hemalatha
@crescent.education
Professor and Dean, School of Life Sciences
B.S.Abdur Rahman Crescent Institute of Science and Technology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
N. Kathija, S. Ranjani, and S. Hemalatha
Springer Science and Business Media LLC
Mahendran Logesh, Kumar Lavanya, Khalil El Mabrouk, Ranjani Soundhararajan, Hemalatha Srinivasan, and A. M. Ballamurugan
Springer Science and Business Media LLC
Shabnam Ameenudeen and S. Hemalatha
World Scientific Pub Co Pte Ltd
Background: Neurodegenerative diseases, including Alzheimer’s disease, are characterized by progressive neuronal cell loss and nervous system damage. Neuropathic pain, resulting from traumatic injury to sensory nerves, is closely associated with these neurodegenerative conditions. Endoplasmic reticulum (ER) stress, arising from protein misfolding or accumulation of unfolded proteins, plays a crucial role in maintaining cellular homeostasis through the activation of the unfolded protein response (UPR). Interestingly, the ER stress pathway has emerged as a potential link between neuropathic pain and Alzheimer’s disease. To investigate this link, we selected specific drugs with known mechanisms of action: GSK2606414, an EIF-2[Formula: see text] phosphorylation inhibitor, Valsartan, which down-regulates ER stress-related proteins, Gemigliptin, known to decrease GRP78 expression, and Melatonin, shown to reduce ATF6 mRNA and protein levels. Additionally, Vemurafenib and Obatoclax were chosen as kinase and Bcl2 inhibitors, respectively. Drug selection was based on data from DrugBank and literature, with their structures obtained from the PubChem database. Main methodologies: Our research aims to identify potential therapeutic targets bridging neuropathic pain and Alzheimer’s disease. Using AutoDock v4.2.6, we conducted molecular docking studies, revealing promising drug–target interactions. We further performed molecular dynamic (MD) simulations for 100 ns using the WEBGRO online server to assess drug-target stability. Additionally, we employed Lipinski screening via the SwissADME web server, assessing pharmacokinetic properties and toxicity. Results: Except for ATF4 (with Metformin), all targets showed favorable affinity toward Obatoclax, with binding energies ranging from −5.32 to −7.48 kcal/mol. The XBP1–Obatoclax complex stability is evaluated using MD simulation. The ADMET screening results indicated that the drugs displayed good intestinal absorption (above 30%), making them suitable for oral administration. Importantly, these drugs showed no inhibitory effects against CYP2D6 isozymes expressed in the CNS. Future perspective: Our in silico findings provide valuable evidence and potential leads for further evaluation and confirmation through in vitro research. The data generated from this study may contribute to the development of novel therapeutics targeting both neuropathic pain and Alzheimer’s disease, offering new hope for patients suffering from these debilitating conditions.
P. Aarthi, Zeba Biswas, S. Hemalatha, and I. Faridha Begum
Wiley
AbstractThe water pollution due to tannery industries that contaminate river water and other spheres, is a side effect of industry expansion. The research focused on the biological removal of synthetic azo dyes by using biological treatment under aerobic conditions. Through morphological observation, biochemical test, and 16S rRNA sequence analysis the isolated bacteria were determined to be Aeromonas caviae, Enterobacter sichuanensis. Reduction of the organic content present in the tannery effluent and removal of the nutrients that cause pollution or inactivate potential pathogenic microorganisms or parasites. Optimized the azo dye degradation conditions at the temperature of 37°C, at pH 7 in 10% inoculum concentration at the time of 48th hour where novel organism capable of performing effective degradation. The metabolite of an aliquot mixture of the optimized condition was analyzed using Fourier Transform Infrared (FTIR) and the results confirmed the reduction of azo dyes. These results indicate that A. caviae and E. sichuanensis can be utilized as a solution for the remediation of tannery industrial effluent containing azo dyes.
Mangala Lakshmi Ragavan and S. Hemalatha
Springer Science and Business Media LLC
Ranjani Soundhararajan, Maroudam Veerasami, Sheeza Khan, Asimul Islam, and Hemalatha Srinivasan
Elsevier BV
Mohamed Juvad NajeerAhamed, Ranjani Soundharajan, and Hemalatha Srinivasan
Elsevier BV
Noorul Samsoon Maharifa Haja Mohaideen, S. Vaani, and S. Hemalatha
Springer Science and Business Media LLC
Shabnam Ameenudeen and Hemalatha Srinivasan
Springer Science and Business Media LLC
S. Ranjani and S. Hemalatha
Springer Science and Business Media LLC
E. M. Love and S. Hemalatha
Springer Science and Business Media LLC
Hariharan Madhumitha, Soundhararajan Ranjani, Jenin Ravindranath Karunyaa, and Srinivasan Hemalatha
Archives of Breast Cancer
Background: Green silver nanoparticles offer a reliable and secure alternative to drugs and prevent cancer. The floral part of Clitoria ternatea is traditionally well known for its application as a medicine and food in various cultures around the world. Phytochemicals of these floral extracts are enriched with various properties. Floral extracts can be utilized as an alternative that can target the proteins and other molecules involved in the progression of cancer. Methods: Silver nanoparticles (CT-AgNPs) were synthesized from the extracts of blue flowers of Clitoria ternatea (CT). The synthesized CT-AgNPs were characterized by various physicochemical methods that revealed the size, shape, and stability of the nanoparticles. Docking was performed between the phytocompounds of Clitoria ternatea and apoptotic proteins involved in breast cancer such as APAF-1, BCL-2, and BAX, to determine the ability of phytocompounds present in the floral extract to control breast cancer by binding with the targets. Results: Based on the docking results, the binding energies were ranging from -6.2 Kcal/mol to -7 Kcal/mol with Quercetin having the highest binding energies. Toxicity analysis of CT-AgNPs in Artermia nauplii and Vigna radiata seedlings confirmed that these nanoparticles were not toxic to both the model systems. Free radical scavenging activity assay revealed the antioxidant nature of CT-AgNPs were similar to that of standard ascorbic acid. In vitro cytotoxicity analysis by using MCF-7 breast cancer cell lines revealed that CT-AgNPs were cytotoxic. Conclusion: In vitro antioxidant and cytotoxicity analysis by using MCF-7 breast cancer cell lines revealed that CT-AgNPs were potent antioxidant and cytotoxic, correlating with the results of in silico analysis and hence demonstrating the anticancer potential of Clitoria ternatea floral mediated nanoparticles.
Jambulingam Vishal, Soundharajan Ranjani, Ravindranath Jenin Karunya, and Srinivasan Hemalatha
Archives of Breast Cancer
Background: Syzygium aromaticum, also known as clove, and its essential oil has already been proved to have antioxidant, anti-inflammatory and anticancer properties. Clove is used in various foods owing to its potent antimicrobial and antioxidant properties. Essential oil extracted from clove has been used in traditional medicine for treating various ailments. Methods: In silico analyses of phytocompounds of Syzygium aromaticum namely eugenol, B-caryophyllene, gallic acid, crategolic acid, kaempferol, quercetin, cinnamaldehyde, and oleanolic acid were docked with three apoptotic proteins involved in breast cancer, namely BCL-2, BAX and APAF-1 using AUTODOCK. In addition, flower bud extract of Syzygium aromaticum was used for the synthesis of AgNPs (silver nanoparticles). The synthesized clove-silver nanoparticles were then characterized using various techniques such as Ultraviolet-visible spectrophotometry, FTIR, FESEM-EDX, DLS and zeta potential to determine the particle size, shape, crystalline structure, and stability of CL-AgNPs and tested for its anticancer potential in MCF-7 cell lines. Results: In silico analysis predicted that phytochemicals of clove have good interactions with the apoptosis related proteins of breast cancer. In vitro assay confirmed the cytotoxic effect of the synthesized CL-AgNPs on breast cancer cells using the MCF-7 cell line with the IC50 value of 58.64 µg/ml. Conclusion: In vitro analysis of the anticancer activity of CL-AgNPs in MCF-7 cell line supports the in silico study by proving active interactions between the phytochemicals of clove and target proteins of the breast cancer and hence Syzygium aromaticum has been proved to possess potential anticancer property. Further research is needed to consider clove-silver nanoparticles as a novel drug for treating breast cancer.
S. Ranjani and S. Hemalatha
Springer Science and Business Media LLC
Asma Baig, Ranjani S, and Hemalatha Srinivasan
Springer Science and Business Media LLC
Antibiotic resistance has become one of the leading causes of death globally. Nigella sativa seeds have seen surge in demand for medicinal applications owing to their bioactive properties. We performed an in-silico study to find whether Nigella sativa phytocompounds inhibit the virulent CTX-M-15 protein, widely found in extended spectrum beta-lactamase (ESBL) producing bacteria. Molecular docking revealed that the phytocompounds showed stronger binding interactions than standard drug Ampicillin. Considering the antibacterial nature of silver nanoparticles (AgNPs), an in-vitro study was performed, involving green synthesis of AgNPs using Nigella sativa seeds, its characterization using various techniques and its antibacterial activity evaluation. The efficiency of the Nigella sativa AgNPs against different strains of Escherichia coli and Klebsiella pneumoniae was tested successfully using antibacterial assays. The combined in-silico and in-vitro studies suggest that Nigella sativa AgNPs exhibit efficient antibacterial activity and could be a probable alternative therapeutic against ESBL producing bacterial infections. Nigella sativa mediated nanoparticles break antibiotic resistance Nigella sativa mediated nanoparticles break antibiotic resistance Nigella sativa mediated nanoparticles break antibiotic resistance
V Santhana lakshmi, S Ranjani, and S Hemalatha
Elsevier BV
Namita Nanda, Ranjani S, and Hemalatha S
Springer Science and Business Media LLC
S. Vaijayanthi, S. Ranjani, and S. Hemalatha
Elsevier BV
M. Love Edet and S. Hemalatha
Springer Science and Business Media LLC
K. Srividhyaa, S. Ranjani, and S. Hemalatha
Springer Science and Business Media LLC
Maruthanayagam Veerabadhran, Nagarajan Manivel, Barathkumar Sarvalingam, Boopathi Seenivasan, Hemalatha Srinivasan, MubarakAli Davoodbasha, and Fei Yang
Elsevier BV
Soniya Ashok Kumar, Noorul Samsoon Maharifa Haja Mohaideen, and Hemalatha S
Springer Science and Business Media LLC
Karunya Jenin Ravindranath, Simon Durairaj Christian, and Hemalatha Srinivasan
Springer Science and Business Media LLC
Manikandan K, Lavanya S, Ranjani S, Faridha Begum, and Hemalatha S
Wiley
AbstractScarcity of water is becoming a big issue. Most of the water is used in industrial sectors including pharmaceutical, food, textiles, leather, and cosmetics. Industrial effluent water is unable to treat and reuse due to their intricate nature. Effluent water contains synthetic azo dyes since they employ synthetic azo dyes as a major coloring agent. Existing approaches are ineffective in treating effluent containing synthetic azo dyes. Synthetic azo dyes containing effluent water can be treated biologically to overcome the drawbacks of physical and chemical processes. In this research work, novel organism was isolated from tannery effluent water. Isolated organisms were screened and selected for effective degradation of azo dyes. One such organism is Citrobacter freundii which effectively degrade the effluent water containing synthetic azo dyes and allowing it to reuse for variety of purpose. Phytotoxicity study of the treated water was carried out and the study confirms non toxicity nature of the treated water. In‐silico screening was carried out to test the interaction efficacy of enzymes such as Laccase, Azo reductase, and peroxidase which are responsible for effective dye degradation with synthetic azo dyes. In silico studies showed better interactions between synthetic azo dyes and enzymes. This is the novel report on showing the effective degradation of synthetic azo yellow dye by using C. freundii isolated from tannery effluent.
Mangala Lakshmi Ragavan and Hemalatha Srinivasan
Informa UK Limited
Drynaria quercifolia is one of the pioneer medicinal plants which exert many beneficial effects on humans. Fatty acids are hydrophobic ligands that act as membrane substrates, metabolic signalling molecules, and metabolic energy sources. It could enhance the mucus production in the intestine which maintain mucosal homeostasis. The inflammatory responses were also regulated by 5-HT receptors. Serotonin 2 A receptors are G-protein coupled receptors targeted by various types of ligands viz. antidepressants, antipsychotics and anti-migraine drugs. The interaction of mucin protein and fatty acid could increase the antimicrobial activity and anti-inflammatory activity of gut microbiome. In this study, dietary phytochemicals were extracted from D. quercifolia and characterized using GC-MS analysis. The result shows presence of 38 various compounds viz. decanoic acid, indole, and dodecanoic acid and etc., Among the all, dodecanoic acid showed good drug likeness and pharmaceutical properties. Target proteins viz. 3IFB (Intestinal fatty acid binding protein) and 7WC4 (5-hydroxytryptamine receptor 2 A) were docked with dodecanoic acid using Auto dock software. The fatty acids produced in the gut could interact with 3IFB and 7WC4 proteins to maintain intestinal integrity and improved gut-brain function respectively. Dodecanoic acid exhibits the highest binding energy with mutated 3IFB (-6.01) than native whereas 7WC4 native protein showed (-7.3 kal/mol) the highest affinity than mutated protein. Structural changes were predicted by using SOPMA and ProSA. Evaluation results indicate that 3IFB was having more stability, good quality, and enhanced affinity than 7WC4. Further, MD simulations were done for 3IFB to check the stability of protein-ligand complex using WebGro platform. The model was investigated by root mean square deviation and fluctuations. Therefore, dodecanoic acids have been considered as a potential agonists and offer opportunities for developing innovative medications for gastrointestinal diseases.Communicated by Ramaswamy H. Sarma.