Bacteriophage therapy, Alternative therapy, Antimicrobial Resistance, Proteus mirabilis
28
Scopus Publications
490
Scholar Citations
12
Scholar h-index
12
Scholar i10-index
Scopus Publications
Molecular Characterization and Clonal Analysis of Carbapenem-Resistant Acinetobacter baumannii: Insights Into Biofilm-Related Gene Coexistence in Clinical Isolates Mahtab Hadadi, Bahram Nasr Esfahani, Arezoo Mirzaei, Sharareh Moghim Biomed Research International, 2026 The emergence of multidrug‐resistant Acinetobacter baumannii (MDR A. baumannii ) and biofilm‐producing ability have become a worldwide serious concern. This study is aimed at investigating the clonal relationships, coexistence of carbapenemase‐resistant and biofilm‐related genes, and biofilm biomass capacity in 57 A. baumannii isolates obtained from patients in intensive care units (ICUs). Antibiotic resistance patterns to 11 antibiotics were determined using the disc diffusion test. The minimum inhibitory concentrations (MICs) of imipenem and colistin were evaluated by the microdilution method. All isolates were subjected to PCR for the detection of carbapenemase‐ and biofilm‐related genes and examined for the biofilm‐forming ability using crystal violet staining methods. The clonality relationship was identified by rep‐PCR. Overall, 49 (86%) isolates were characterized as extensively drug‐resistant (XDR) with a high MIC for imipenem. Eight isolates were resistant to colistin (MIC>64 μ g/mL). Additionally, 86.21% of isolates were strong biofilm formers, which correlated with the PDR phenotype. All isolates carried at least three genes related to biofilm formation. Genotypically, 100% of isolates had bla OXA−51-like , bla OXA−24−like , and bla TEM genes, followed by bla VIM (61.4%), bla OXA-23-like (24.6%), bla SHV (1.8%), and bla KPC (1.8%), whereas bla CTX-M and bla OXA-58-like genes were not found in the isolates. The rep‐PCR analysis identified 10 distinct genotypes, among which GTG Type 3 showed a significant correlation with strong biofilm formation. Moreover, the greatest number of colistin‐resistant isolates (MIC>64 μ g/mL) were located in this cluster. This study highlights the emergence of PDR A. baumannii strains carrying a variety of β ‐lactamase and biofilm‐related genes in ICUs, underscoring the urgent need for improved infection control measures and antimicrobial stewardship programs to address the spread of these formidable pathogens.
Mupirocin: A Useful Antibiotic for Methicillin-Resistant Staphylococcus aureus Decolonization: A Narrative Review Yasin Saberi, Kasra Javadi, Arezoo Mirzaei, M. Halaji Microbial Drug Resistance, 2025 Mupirocin (MUP) is a topical antibiotic derived from Pseudomonas fluorescens, widely used for the treatment of superficial skin infections and decolonization of methicillin-resistant Staphylococcus aureus (MRSA). Its unique mechanism of action, selective inhibition of bacterial isoleucyl-transfer RNA synthetase, confers high specificity with minimal risk of cross-resistance to other antibiotic classes. This narrative review provides an updated overview of MUP's chemical structure, mechanism of action, clinical efficacy, resistance mechanisms, and global resistance trends based on literature published from 2017 to 2024. Recent clinical studies confirm MUP's continued effectiveness, particularly in the decolonization of MRSA nasal infections. However, resistance, including high-level MUP resistance mediated by the mupA and mupB genes, is increasingly reported worldwide. A better understanding of resistance patterns and judicious use of MUP is essential to preserving its clinical utility in the context of rising antimicrobial resistance.
Alhagi maurorum extract in combination with lytic phage cocktails: a promising therapeutic approach against biofilms of multi-drug resistant P. mirabilis Arezoo Mirzaei, Bahram Nasr Esfahani, Mustafa Ghanadian, Jeroen Wagemans, Rob Lavigne, et al. Frontiers in Pharmacology, 2024 Antimicrobial resistance (AMR) poses a significant global threat to public health systems, rendering antibiotics ineffective in treating infectious diseases. Combined use of bio compounds, including bacteriophages and plant extracts, is an attractive approach to controlling antibiotic resistance. In this study, the combination of phage cocktail (Isf-Pm1 and Isf-Pm2) and Alhagi maurorum crude extract (AME) was investigated in controlling biofilm-forming multi-drug resistant P. mirabilis isolates, in vitro and a phantom bladder model. The combination of AME and phage cocktails demonstrated no significant disparity in its ability to inhibit quorum sensing (QS) when compared to the individual control of AME alone. Following treatment with the combination of phage cocktail and AME at a 125 μg/mL concentration, the MDR P. mirabilis biofilm biomass was notably reduced by 73% compared to the control (P< 0.0001). The anti-biofilm effect was confirmed by Scanning Electron Microscopy (SEM). Moreover, in a bladder phantom model, there was a considerable decrease in encrustation levels compared to the control. The combined treatment resulted in a 1.85 logarithmic reduction in bacterial adhesion to Vero cells compared to the control. The real-time PCR results indicated significant downregulation of QS- and adhesion-related gens. The phage therapy, combined with AME, holds promising potential in reducing biofilm formation.
Flavonoids Isolated From Delphinium semibarbatum Flowering Aerial Parts With Their Antibacterial, Antibiofilm, and Antiswarming Activity Against Proteus mirabilis and Staphylococcus aureus Mohammadreza Lotfaliani, Seyed Abdulmajid Ayatollahi, Farzad Kobarfard, Mohammad Bagher Majnooni, Arezoo Mirzaei, et al. Journal of Food Biochemistry, 2024 Proteus mirabilis and Staphylococcus aureus are pathogens associated with CAUTIs. Understanding their significance is crucial for effective prevention and treatment. In this study, the Delphinium semibarbatum flavonoid‐rich fraction (DSF) was analyzed, and its antibacterial, antibiofilm, and antiswarming properties against these two bacterial species were evaluated. In the phytochemical analysis, four flavonoids, including kaempferol, 4′‐O‐methyl quercetin, quercetin, and kaempferol‐3‐O‐beta‐D‐glucopyranoside (K3G), were isolated. The minimum inhibitory concentration (MIC50) of DSF on P. mirabilis and S. aureus was 1000 μg/mL. Similarly, kaempferol, K3G, and 4′‐O‐methyl quercetin exhibited inhibitory effects of 20%, 13%, and 12%, respectively, at a concentration of 1000 μg/mL compared to the P. mirabilis control. However, the MIC50 of kaempferol and 4′‐O‐methyl quercetin for S. aureus were 125 μg/mL and 62.25 μg/mL, respectively. K3G exhibited an inhibitory effect of 37% at a concentration of 1000 μg/mL. DSF reduced biofilm at 1 mg/mL by 78% and 74% for P. mirabilis and S. aureus, respectively. Quercetin was the most effective in inhibiting P. mirabilis biofilm formation, with a 96% inhibition rate, followed by kaempferol and K3G, with 67% and 29%, respectively, at the same concentration. The best bioactive compound against biofilm inhibition of S. aureus was quercetin at 250 μg/mL, exhibiting 90% inhibition, followed by K3G, 4′‐O‐methyl quercetin, and kaempferol with 81%, 45%, and 33%, respectively, at a concentration of 125 μg/mL. In the swarming inhibition assay, DSF showed 78% inhibition at concentrations of 5 μg/mL. Similarly, all bioactive compounds showed 45% antiswarming activity at this concentration. The docking results of the isolated flavonoids on two target proteins, SarA and MrpH, showed that the presence of the methoxy group in the structure of 4′‐O‐methyl quercetin reduced its interaction with these two proteins. In the ADMET prediction, isolated flavonoids showed good predicted properties suitable for treating these two microbial species in urinary infection diseases.
Correction to: Investigating the therapeutic effects of caffeine‑naringenin combination on the repair of intestinal permeability and composition of gut microbiota in obese rats … A Dehghani, A Mirzaei, M Malakoutikhah, P Karimi, F Zadhoush, ... Naunyn-Schmiedeberg's archives of pharmacology , 2026 2026
Investigating the therapeutic effects of caffeine-naringenin combination on the repair of intestinal permeability and composition of gut microbiota in obese rats under long … A Dehghani, A Mirzaei, M Malakoutikhah, P Karimi, F Zadhoush, ... Naunyn-Schmiedeberg's Archives of Pharmacology, 1-15 , 2026 2026
Molecular Characterization and Clonal Analysis of Carbapenem‐Resistant Acinetobacter baumannii : Insights Into Biofilm‐Related Gene Coexistence in Clinical … M Hadadi, BN Esfahani, A Mirzaei, S Moghim BioMed Research International 2026 (1), 2304337 , 2026 2026 Citations: 1
Mupirocin: A Useful Antibiotic for Methicillin-Resistant Staphylococcus aureus Decolonization: A Narrative Review Y Saberi, K Javadi, A Mirzaei, M Halaji Microbial Drug Resistance 31 (8), 250-261 , 2025 2025 Citations: 4
Investigating the effect of rAzurin loaded mesoporous silica nanoparticles enwrapped with chitosan-folic acid on breast tumor regression in BALB/C mice HM Barzelighi, B Bakhshi, B Daraei, A Mirzaei International Journal of Biological Macromolecules 300, 139245 , 2025 2025 Citations: 4
Clonal repetitive element polymerase chain reaction patterns of Pseudomonas aeruginosa in diabetic foot ulcers, Iran N Ghahari, A Mirzaei, BN Esfahani, S Moghim Ijid Regions 14, 100557 , 2025 2025 Citations: 3
Alhagi maurorum extract in combination with lytic phage cocktails: a promising therapeutic approach against biofilms of multi-drug resistant P. mirabilis A Mirzaei, BN Esfahani, M Ghanadian, J Wagemans, R Lavigne, ... Frontiers in Pharmacology 15, 1483055 , 2024 2024 Citations: 6
Antibiotic profile classification of Proteus mirabilis using machine learning: An investigation into multidimensional radiomics features A Khorasani, S Moghim, J Wagemans, R Lavigne, A Mirzaei Computers in biology and medicine 182, 109131 , 2024 2024 Citations: 7
Gut microbiome composition and dysbiosis in immune thrombocytopenia: a review of literature N Saki, H Hadi, B Keikhaei, A Mirzaei, D Purrahman Blood Reviews 67, 101219 , 2024 2024 Citations: 12
Flavonoids Isolated From Delphinium semibarbatum Flowering Aerial Parts With Their Antibacterial, Antibiofilm, and Antiswarming Activity Against Proteus mirabilis and … M Lotfaliani, SA Ayatollahi, F Kobarfard, MB Majnooni, A Mirzaei, ... Journal of Food Biochemistry 2024 (1), 9994944 , 2024 2024 Citations: 1
The antimicrobial effects of Lactobacillus plantarum and Lactobacillus rhamnosus isolated from local kefir buttermilk on oral pathogens. KS Ekhteraei, F Poursina, A Mirzaei, MS Damavandi, T Narimani 2023
Antimicrobial activity and wound healing effect of a novel natural ointment: an in vitro and in vivo study D Khodabakhshi, G Vaseghi, A Mirzaee, A Eskandarinia, AZ Kharazi Journal of wound care 32 (Sup6), S18-S26 , 2023 2023 Citations: 8
Antibacterial, Antibiofilm, Antiswarming, and Antioxidant Activities of Flavonoids Isolated from Allium colchicifolium Leaves MB Majnooni, SM Ghanadian, M Mojarrab, G Bahrami, K Mansouri, ... Journal of Food Biochemistry 2023 (1), 5521661 , 2023 2023 Citations: 13
Enteroviruses and Parechovirus in Children with Acute Respiratory Infections: A Survey in Isfahan, Iran S Sadri, A Mirzaei, B Nar Esfahani, M Khalili, S Moghim Journal of Microbial Biology 11 (44), 119-127 , 2022 2022
A phage cocktail to control surface colonization by proteus mirabilis in catheter-associated urinary tract infections A Mirzaei, J Wagemans, B Nasr Esfahani, R Lavigne, S Moghim Microbiology Spectrum 10 (5), e02092-22 , 2022 2022 Citations: 25
Alhagi maurorum extract modulates quorum sensing genes and biofilm formation in Proteus mirabilis A Mirzaei, B Nasr Esfahani, M Ghanadian, S Moghim Scientific Reports 12 (1), 13992 , 2022 2022 Citations: 28
The characteristic and potential therapeutic effect of isolated multidrug-resistant Acinetobacter baumannii lytic phage B Sisakhtpour, A Mirzaei, V Karbasizadeh, N Hosseini, M Shabani, ... Annals of clinical microbiology and antimicrobials 21 (1), 1 , 2022 2022 Citations: 59
Cellular miR-101-1 reduces efficiently the replication of HSV-1 in HeLa cells B Sadegh Ehdaei, A Pirouzmand, M Shabani, A Mirzaei, S Moghim Intervirology 64 (2), 88-95 , 2021 2021 Citations: 12
Efficacy of isolated bacteriophage against biofilm embedded colistin-resistant Acinetobacter baumannii S Ebrahimi, B Sisakhtpour, A Mirzaei, V Karbasizadeh, S Moghim Gene Reports 22, 100984 , 2021 2021 Citations: 26
From the urinary catheter to the prevalence of three classes of integrons, β‐lactamase genes, and differences in antimicrobial susceptibility of Proteus mirabilis and clonal … A Mirzaei, B Nasr Esfahani, A Raz, M Ghanadian, S Moghim BioMed research international 2021 (1), 9952769 , 2021 2021 Citations: 59
MOST CITED SCHOLAR PUBLICATIONS
Characterization of Antibiotic-Susceptibility Patterns, Virulence Factor Profiles and Clonal Relatedness in Proteus mirabilis Isolates from Patients with Urinary Tract … A Mirzaei, M Habibi, S Bouzari, MR Asadi Karam Infection and drug resistance, 3967-3979 , 2019 2019 Citations: 84
The characteristic and potential therapeutic effect of isolated multidrug-resistant Acinetobacter baumannii lytic phage B Sisakhtpour, A Mirzaei, V Karbasizadeh, N Hosseini, M Shabani, ... Annals of clinical microbiology and antimicrobials 21 (1), 1 , 2022 2022 Citations: 59
From the urinary catheter to the prevalence of three classes of integrons, β‐lactamase genes, and differences in antimicrobial susceptibility of Proteus mirabilis and clonal … A Mirzaei, B Nasr Esfahani, A Raz, M Ghanadian, S Moghim BioMed research international 2021 (1), 9952769 , 2021 2021 Citations: 59
The Global Prevalence of Class 1 Integron and Associated Antibiotic Resistance in Escherichia coli from Patients with Urinary Tract Infections, a Systematic Review … M Halaji, A Feizi, A Mirzaei, H Sedigh Ebrahim-Saraie, A Fayyazi, ... Microbial Drug Resistance 26 (10), 1208-1218 , 2020 2020 Citations: 50
Characterization of virulence factors, antimicrobial resistance patterns and biofilm formation of Pseudomonas aeruginosa and Staphylococcus spp. strains isolated from corneal … H Heidari, M Hadadi, HS Ebrahim-Saraie, A Mirzaei, A Taji, SR Hosseini, ... Journal francais d'ophtalmologie 41 (9), 823-829 , 2018 2018 Citations: 47
Alhagi maurorum extract modulates quorum sensing genes and biofilm formation in Proteus mirabilis A Mirzaei, B Nasr Esfahani, M Ghanadian, S Moghim Scientific Reports 12 (1), 13992 , 2022 2022 Citations: 28
Efficacy of isolated bacteriophage against biofilm embedded colistin-resistant Acinetobacter baumannii S Ebrahimi, B Sisakhtpour, A Mirzaei, V Karbasizadeh, S Moghim Gene Reports 22, 100984 , 2021 2021 Citations: 26
A phage cocktail to control surface colonization by proteus mirabilis in catheter-associated urinary tract infections A Mirzaei, J Wagemans, B Nasr Esfahani, R Lavigne, S Moghim Microbiology Spectrum 10 (5), e02092-22 , 2022 2022 Citations: 25
Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro M Shabani, BN Esfahani, BS Ehdaei, S Moghim, A Mirzaei, M Sharifi, ... Research in pharmaceutical sciences 14 (2), 167-174 , 2019 2019 Citations: 16
Antibacterial, Antibiofilm, Antiswarming, and Antioxidant Activities of Flavonoids Isolated from Allium colchicifolium Leaves MB Majnooni, SM Ghanadian, M Mojarrab, G Bahrami, K Mansouri, ... Journal of Food Biochemistry 2023 (1), 5521661 , 2023 2023 Citations: 13
Gut microbiome composition and dysbiosis in immune thrombocytopenia: a review of literature N Saki, H Hadi, B Keikhaei, A Mirzaei, D Purrahman Blood Reviews 67, 101219 , 2024 2024 Citations: 12
Cellular miR-101-1 reduces efficiently the replication of HSV-1 in HeLa cells B Sadegh Ehdaei, A Pirouzmand, M Shabani, A Mirzaei, S Moghim Intervirology 64 (2), 88-95 , 2021 2021 Citations: 12
New insight into laboratory tests and imaging modalities for fast and accurate diagnosis of COVID‐19: alternative suggestions for routine RT‐PCR and CT—A literature review A Khorasani, A Chegini, A Mirzaei Canadian Respiratory Journal 2020 (1), 4648307 , 2020 2020 Citations: 9
Antimicrobial activity and wound healing effect of a novel natural ointment: an in vitro and in vivo study D Khodabakhshi, G Vaseghi, A Mirzaee, A Eskandarinia, AZ Kharazi Journal of wound care 32 (Sup6), S18-S26 , 2023 2023 Citations: 8
The case report of Mycobacterium arupense wound infection in diabetes mellitus patients; the first report and literature review S Navid, B Sadegh-Ehdaei, M Shabani, M Hasani, A Mirzaei, K Ghazvini, ... Access Microbiology 2 (4), e000106 , 2020 2020 Citations: 8
Antibiotic profile classification of Proteus mirabilis using machine learning: An investigation into multidimensional radiomics features A Khorasani, S Moghim, J Wagemans, R Lavigne, A Mirzaei Computers in biology and medicine 182, 109131 , 2024 2024 Citations: 7
Alhagi maurorum extract in combination with lytic phage cocktails: a promising therapeutic approach against biofilms of multi-drug resistant P. mirabilis A Mirzaei, BN Esfahani, M Ghanadian, J Wagemans, R Lavigne, ... Frontiers in Pharmacology 15, 1483055 , 2024 2024 Citations: 6
Mupirocin: A Useful Antibiotic for Methicillin-Resistant Staphylococcus aureus Decolonization: A Narrative Review Y Saberi, K Javadi, A Mirzaei, M Halaji Microbial Drug Resistance 31 (8), 250-261 , 2025 2025 Citations: 4
Investigating the effect of rAzurin loaded mesoporous silica nanoparticles enwrapped with chitosan-folic acid on breast tumor regression in BALB/C mice HM Barzelighi, B Bakhshi, B Daraei, A Mirzaei International Journal of Biological Macromolecules 300, 139245 , 2025 2025 Citations: 4
Misleading of the diagnosis of Mycobacterium attributed lung diseases to malignancy due to smear, culture and PCR negative results: A lesson from a case report S Navid, S Arzhang, A Mirzaei, A Kefayat, Z Naderi Indian Journal of Tuberculosis 67 (3), 371-373 , 2020 2020 Citations: 4