@lincoln.edu.my
Lecturer in Biotechnology Department, Faculty of Applied Sciences, Lincoln University College
Lincoln University College
2018–22: Universiti Malaya (UM), PhD in Biochemistry and Biology
2014–18: Universiti Malaya (UM), BSc in Biochemistry
2007–13: Catholic High School, Petaling Jaya (SPM and STPM)
Natural products, in vitro drug discovery, biochemistry
Scopus Publications
Nur Husnaini Basir, Abdul Qaiyum Ramle, Min Phin Ng, Chun Hoe Tan, Edward R.T. Tiekink, Kae Shin Sim, Wan Jefrey Basirun, and Melati Khairuddean
Elsevier BV
Sin-Yee Tang, Min-Phin Ng, Chun-Hoe Tan, Kae-Shin Sim, Kuan-Hon Lim, Kien-Thai Yong, Yun-Yee Low, and Siew-Huah Lim
Elsevier BV
Sin-Yee Tang, Chun-Hoe Tan, Kae-Shin Sim, Kien-Thai Yong, Kuan-Hon Lim, Yun-Yee Low, and Siew-Huah Lim
Elsevier BV
Nur Amira Solehah Pungut, Chun Hoe Tan, Hazwani Mat Saad, Kae Shin Sim, Sheena Yin Xin Tiong, Chee Wei Ang, Chun Hau Gan, Kien Voon Kong, and Kong Wai Tan
Elsevier BV
Wei Chuen Chan, Min Phin Ng, Chun Hoe Tan, Chee Wei Ang, Kae Shin Sim, Sheena Yin Xin Tiong, Nur Amira Solehah Pungut, Chew Hee Ng, and Kong Wai Tan
Elsevier BV
Hazwani Mat Saad, Chun Hoe Tan, Sugumaran Manickam, Siew Huah Lim, and Kae Shin Sim
Penerbit Universiti Kebangsaan Malaysia (UKM Press)
Natural-based skin-lightening cosmeceutical products are attracting high popularity nowadays due to their relatively high bioavailability upon application. Artocarpus species have been highlighted with such potential, and our previous studies have reported that Artocarpus heterophyllusLam. stem bark extract exhibited a potent anti-melanogenic activity by reducing melanin content and inhibiting cellular tyrosinase activity in B16F10 melanoma cells. Hence, this study aimed to identify the bioactive fraction from A. heterophyllus Lam. stem bark and determine its anti-melanogenic mechanisms in B16F10 melanoma cells. Our results showed that a fraction (H-3) demonstrated the most pronounced anti-melanogenic effect at 12.00 µg/mL by reducing melanin content to 22.86 ± 2.90% and inhibiting cellular tyrosinase activity at treatment concentration 33-fold lower than kojic acid, without being cytotoxic against B16F10 melanoma cells. Moreover, treatment with H-3 for 24 and 48 h substantially scavenged intracellular reactive oxygen species (ROS) of hydrogen peroxide-challenged B16F10 melanoma cells by 1.8 and 4.4%, respectively. Based on the microarray profiling and qPCR analysis, H-3 downregulated Creb3l1, Creb3l2, Creb3l3, Mitf, Tyr, Tyrp1, and Dct genes in B16F10 melanoma cells, whereas the expression of Map3k20, Mapk14 (p38), and Foxo3 genes was markedly increased. Altogether, these results demonstrated that H-3 exhibited its anti-melanogenic activity in B16F10 melanoma cells through scavenging ROS and concurrent inhibition of the cAMP and activation of the p38/MAPK signaling pathways. These findings indicate that H-3 has the potential to be used as a skin lightening cosmeceutical agent in the treatment of skin hyperpigmentation.
Abdul Qaiyum Ramle, Nadia Nabihah Mohd Yusof Chan, Min Phin Ng, Chun Hoe Tan, Kae Shin Sim, Edward R. T. Tiekink, and Chee Chin Fei
Springer Science and Business Media LLC
Chun Hoe Tan, Dawn Su Yin Sim, Siew Huah Lim, Taznim Begam Mohd Mohidin, Gokula Mohan, Yun Yee Low, Toh Seok Kam, and Kae Shin Sim
Georg Thieme Verlag KG
AbstractTwo iboga-vobasine bisindoles, 16′-decarbomethoxyvoacamine (1) and its 19,20-dihydro derivative, 16′-decarbomethoxydihydrovoacamine (2) from Tabernaemontana corymbosa exhibited potent cytotoxicity against the human colorectal adenocarcinoma HT-29 cells in our previous studies. Bisindoles 1 and 2 selectively inhibited the growth of HT-29 cells without significant cytotoxicity to normal human colon fibroblasts CCD-18Co. Treatment with bisindoles 1 and 2 suppressed the formation of HT-29 colonies via G0/G1 cell cycle arrest and induction of mitochondrial apoptosis. Owing to its higher antiproliferative activity, bisindole 2 was chosen for the subsequent studies. Bisindole 2 inhibited the formation of HT-29 spheroids (tumor-like cell aggregates) in 3D experiments in a dose-dependent manner, while an in vitro tubulin polymerization assay and molecular docking analysis showed that bisindole 2 is a microtubule-stabilizing agent which is predicted to bind at the β-tubulin subunit at the taxol-binding site. The binding resulted in the generation of ROS, which consequently activated the oxidative stress-related cell cycle arrest and apoptotic pathways, viz., JNK/p38, p21Cip1/Chk1, and p21Cip1/Rb/E2F, as shown by microarray profiling.
See Mun Lee, Kong Mun Lo, Li Yuan Liew, Jactty Chew, Chun Hoe Tan, Kae Shin Sim, and Edward R.T. Tiekink
Elsevier BV
Shiaw Xian Lee, Chun Hoe Tan, Wee Li Mah, Richard Chee Seng Wong, Ninie Suhana Abdul Manan, Yuen Lin Cheow, Kae Shin Sim, and Kong Wai Tan
Elsevier BV
Chun Hau Gan, Kong Wai Tan, Mei Lee Ooi, Jonathan Wee Kent Liew, Yee Ling Ng, Yee Ling Lau, Yin Zhuang Ng, Chew Hee Ng, Chun Hoe Tan, and Richard C.S. Wong
Elsevier BV
Shiaw Xian Lee, Chun Hoe Tan, Wee Li Mah, Richard Chee Seng Wong, Kae Shin Sim, Yuen Lin Cheow, Chew-Hee Ng, and Kong Wai Tan
Elsevier BV
Savina Savir, Jonathan Wee Kent Liew, Indra Vythilingam, Yvonne Ai Lian Lim, Chun Hoe Tan, Kae Shin Sim, Vannajan Sanghiran Lee, Mohd. Jamil Maah, and Kong Wai Tan
Elsevier BV
Shiaw Xian Lee, Chun Hoe Tan, Wee Li Mah, Richard Chee Seng Wong, Yuen Lin Cheow, Kae Shin Sim, and Kong Wai Tan
Elsevier BV
Hazwani Mat Saad, Syarifah Nur Syed Abdul Rahman, Suerialoasan Navanesan, Chun Hoe Tan, Sugumaran Manickam, Sri Nurestri Abd Malek, and Kae Shin Sim
Elsevier BV
Chun-Hoe Tan, Joanne Soon-Yee Yeap, Siew-Huah Lim, Yun-Yee Low, Kae-Shin Sim, and Toh-Seok Kam
American Chemical Society (ACS)
A new linearly fused macroline-sarpagine bisindole, angustilongine M (1), was isolated from the methanolic extract of Alstonia penangiana. The structure of the alkaloid was elucidated based on analysis of the spectroscopic data, and its biological activity was evaluated together with another previously reported macroline-akuammiline bisindole from the same plant, angustilongine A (2). Compounds 1 and 2 showed pronounced in vitro growth inhibitory activity against a wide panel of human cancer cell lines. In particular, the two compounds showed potent and selective antiproliferative activity against HT-29 cells, as well as strong growth inhibitory effects against HT-29 spheroids. Cell death mechanistic studies revealed that the compounds induced mitochondrial apoptosis and G0/G1 cell cycle arrest in HT-29 cells in a time-dependent manner, while in vitro tubulin polymerization assays and molecular docking analysis showed that the compounds are microtubule-stabilizing agents, which are predicted to bind at the β-tubulin subunit at the Taxol-binding site.
Hazwani Mat Saad, Chun Hoe Tan, Siew Huah Lim, Sugumaran Manickam, and Kae Shin Sim
Elsevier BV
Soon-Kit Wong, Joanne Soon-Yee Yeap, Chun-Hoe Tan, Kae-Shin Sim, Siew-Huah Lim, Yun-Yee Low, and Toh-Seok Kam
Elsevier BV
Chun Hoe Tan, Dawn Su Yin Sim, Mok Piew Heng, Siew Huah Lim, Yun Yee Low, Toh Seok Kam, and Kae Shin Sim
Wiley
Joanne Soon-Yee Yeap, Chun-Hoe Tan, Kien-Thai Yong, Kuan-Hon Lim, Siew-Huah Lim, Yun-Yee Low, and Toh-Seok Kam
Elsevier BV