CHUN HOE TAN

EDUCATION

2018–22: Universiti Malaya (UM), PhD in Biochemistry and Biology
2014–18: Universiti Malaya (UM), BSc in Biochemistry
2007–13: Catholic High School, Petaling Jaya (SPM and STPM)

RESEARCH INTERESTS

Natural products, in vitro drug discovery, biochemistry

Scopus Publications

Computational analysis of antimicrobial phytochemicals from Bambusa vulgaris Schrad. ex J.C.Wendl.: Pharmacokinetics, toxicity, and molecular docking
Asita Elengoe, Chun Hoe Tan, and Sandeep Poddar
Elsevier BV



Investigation of cytotoxic indoleninyl-thiobarbiturate zwitterions as DNA targeting agents
Adebayo Zainab Funmi, Ching Yi Lim, Min Phin Ng, Wen Xin Toh, Yee Seng Tan, Kae Shin Sim, Siew Huah Lim, Chun Hoe Tan, and Abdul Qaiyum Ramle
Elsevier BV



Sialic acid detection and theranostic activity of phenylboronic acid-based fluorescent probe in human colorectal adenocarcinoma HT-29 cells
Min Phin Ng, Wei Chuen Chan, Min Li Tan, Chun Hoe Tan, Sheena Yin Xin Tiong, Kae Shin Sim, and Kong Wai Tan
Elsevier BV



Computational Elucidation of Hub Genes and Pathways Correlated with the Development of 5-Fluorouracil Resistance in HCT 116 Colorectal Carcinoma Cell Line
Chun Hoe Tan, Siew Huah Lim, and Kae Shin Sim
Springer Science and Business Media LLC



Investigation of Phytochemical Composition, Radical Scavenging Potential, Anti-Obesogenic Effects, and Anti-Diabetic Activities of Kaempferia parviflora Rhizomes
Sangeetha Prabakaran, Hazwani Mat Saad, Chun Hoe Tan, Syarifah Nur Syed Abdul Rahman, and Kae Shin Sim
Wiley
AbstractKaempferia parviflora or commonly known as “Kunyit hitam” by locals, is an edible plant, native to tropical regions, has been extensively utilized for culinary and medicinal applications. The present study aimed to investigate the phytochemical composition and biological activities of the rhizomes of K. parviflora. The ethanol crude and fractionated extracts (hexane and chloroform) of the rhizomes were evaluated for their total phenolic content, total steroidal content, as well as antioxidant, anti‐obesogenic, and anti‐diabetic activities. The chloroform extract demonstrated the highest concentration of plant sterols (432±0.23 mg BSE/g extract) and a substantial amount of phenolic compounds (1.19×103±0.41 mg GAE/g extract). Gas chromatography‐mass spectrometry (GC/MS) analysis revealed that the chloroform extract of the rhizomes is predominantly composed of bioactive flavonoids including tectochrysin (1), 5,7‐dimethoxyflavone (2), 3,5,7‐trimethoxyflavone (3), 3,4’,5,7‐tetramethoxyflavone (4), and 4’,5,7‐trimethoxyflavone (5). Furthermore, the chloroform extract exhibited the highest overall radical scavenging and α‐glucosidase inhibitory activities, which can be attributed to the presence of compounds 1–5 in the extract. Collectively, these findings suggest that the chloroform extract of the rhizomes of K. parviflora is a potentially valuable source of bioactive compounds with antioxidant, anti‐obesogenic, and anti‐diabetic properties, with potential application in therapeutics and functional foods.


Revalidation of Reliability and Validity of the Post-Traumatic Stress Disorder Checklist-civilian Version among High-risk Pregnancy Women




Structural insights and cytotoxicity evaluation of benz[e]indole pyrazolyl-substituted amides
Abdul Qaiyum Ramle, Nadia Nabihah Mohd Yusof Chan, Min Phin Ng, Chun Hoe Tan, Kae Shin Sim, Edward R. T. Tiekink, and Chee Chin Fei
Springer Science and Business Media LLC



Discovery of indoleninyl-pyrazolo[3,4-b]pyridines as potent chemotherapeutic agents against colorectal cancer cells
Nur Husnaini Basir, Abdul Qaiyum Ramle, Min Phin Ng, Chun Hoe Tan, Edward R.T. Tiekink, Kae Shin Sim, Wan Jefrey Basirun, and Melati Khairuddean
Elsevier BV








Biological Investigation of Spent Coffee Ground for Potential Cosmeceutical Applications
Amanda Harshana Senarath Mudalige Don, Hazwani Mat Saad, Erlina Abdullah, A. Elengoe, Siew Huah Lim and Chun-Hoe Tan




In Vitro and Computational Studies of Indoleninyl-Pyrimido[1,2-b]Indazoles as DNA Binding Agents
Li Xuan Yap, Abdul Qaiyum Ramle, Kae Shin Sim, and Chun Hoe Tan
Springer Science and Business Media LLC



Computational and In vitro Elucidation of Indolenine-barbituric Acid Zwitterions as Potential Chemotherapeutical Agents
Kang Kit Ong, Abdul Qaiyum Ramle, Min Phin Ng, Siew Huah Lim, Kae Shin Sim, and Chun Hoe Tan
Bentham Science Publishers Ltd.
Introduction: The continuous pursuit of novel chemotherapeutical agents with improved efficacy and reduced adverse effects remains a critical area of research despite advancements in chemotherapy. We have previously synthesized indolenine and barbituric acid zwitterion scaffolds 1–10 sustainably; however, their precise chemotherapeutical properties are still lacking. Methods: In this present work, we conducted in silico ADMET analyses, molecular docking calculations, DNA binding studies, and cytotoxicity assays on these zwitterions. Results and Discussion: Among the 10 zwitterions, zwitterion 3 bearing a methoxy group demonstrated the highest drug-likeness score, low toxicity, as well as no violation of Lipinski’s rule of five and Veber’s rule. Both molecular docking calculations and DNA binding studies suggested that the minor groove of DNA is the most probable molecular target of 3 among the others (i.e., topoisomerase and tubulin). In addition, zwitterion 3 exhibited selective cytotoxicity against a wide array of human cancer cell lines without noticeable effect against the normal human colon fibroblast CCD- 18Co. Conclusion: Overall, these preliminary findings from our combined computational and experimental strategy suggested that 3 remains promising for further elaboration as a chemotherapeutic agent.


Chippiine, iboga, cleavamine, vobasine, and cytotoxic bisindole alkaloids from Tabernaemontana polyneura
Sin-Yee Tang, Min-Phin Ng, Chun-Hoe Tan, Kae-Shin Sim, Kuan-Hon Lim, Kien-Thai Yong, Yun-Yee Low, and Siew-Huah Lim
Elsevier BV



Clinical symptoms and diagnoses of COVID-19




Medicinal plants for SARS-CoV-2 infections




Traditional plant-based therapies for SARS-CoV-2 infections




The pathogenesis of coronavirus through the host




Polyneurines A−H, iboga alkaloids from Tabernaemontana polyneura
Sin-Yee Tang, Chun-Hoe Tan, Kae-Shin Sim, Kien-Thai Yong, Kuan-Hon Lim, Yun-Yee Low, and Siew-Huah Lim
Elsevier BV



A new cinnamaldehyde-rhodamine based dual chemosensor for Cu²⁺ and Fe³⁺ and its applicability in live cell imaging
Nur Amira Solehah Pungut, Chun Hoe Tan, Hazwani Mat Saad, Kae Shin Sim, Sheena Yin Xin Tiong, Chee Wei Ang, Chun Hau Gan, Kien Voon Kong, and Kong Wai Tan
Elsevier BV



A new lipophilic cationic rhodamine-based chemosensor for detection of Al(III)/Cu(II) and intracellular pH change and its application as a smartphone-assisted sensor in water sample analysis
Wei Chuen Chan, Min Phin Ng, Chun Hoe Tan, Chee Wei Ang, Kae Shin Sim, Sheena Yin Xin Tiong, Nur Amira Solehah Pungut, Chew Hee Ng, and Kong Wai Tan
Elsevier BV



Artocarpus heterophyllus Lam. Stem bark inhibits melanogenesis through regulation of ROS, cAMP, and MAPK pathways
Hazwani Mat Saad, Chun Hoe Tan, Sugumaran Manickam, Siew Huah Lim, and Kae Shin Sim
Penerbit Universiti Kebangsaan Malaysia (UKM Press)
Natural-based skin-lightening cosmeceutical products are attracting high popularity nowadays due to their relatively high bioavailability upon application. Artocarpus species have been highlighted with such potential, and our previous studies have reported that Artocarpus heterophyllusLam. stem bark extract exhibited a potent anti-melanogenic activity by reducing melanin content and inhibiting cellular tyrosinase activity in B16F10 melanoma cells. Hence, this study aimed to identify the bioactive fraction from A. heterophyllus Lam. stem bark and determine its anti-melanogenic mechanisms in B16F10 melanoma cells. Our results showed that a fraction (H-3) demonstrated the most pronounced anti-melanogenic effect at 12.00 µg/mL by reducing melanin content to 22.86 ± 2.90% and inhibiting cellular tyrosinase activity at treatment concentration 33-fold lower than kojic acid, without being cytotoxic against B16F10 melanoma cells. Moreover, treatment with H-3 for 24 and 48 h substantially scavenged intracellular reactive oxygen species (ROS) of hydrogen peroxide-challenged B16F10 melanoma cells by 1.8 and 4.4%, respectively. Based on the microarray profiling and qPCR analysis, H-3 downregulated Creb3l1, Creb3l2, Creb3l3, Mitf, Tyr, Tyrp1, and Dct genes in B16F10 melanoma cells, whereas the expression of Map3k20, Mapk14 (p38), and Foxo3 genes was markedly increased. Altogether, these results demonstrated that H-3 exhibited its anti-melanogenic activity in B16F10 melanoma cells through scavenging ROS and concurrent inhibition of the cAMP and activation of the p38/MAPK signaling pathways. These findings indicate that H-3 has the potential to be used as a skin lightening cosmeceutical agent in the treatment of skin hyperpigmentation.


Antiproliferative and Microtubule-stabilizing Activities of Two Iboga-vobasine Bisindoles Alkaloids from Tabernaemontana corymbosa in Colorectal Adenocarcinoma HT-29 Cells
Chun Hoe Tan, Dawn Su Yin Sim, Siew Huah Lim, Taznim Begam Mohd Mohidin, Gokula Mohan, Yun Yee Low, Toh Seok Kam, and Kae Shin Sim
Georg Thieme Verlag KG
AbstractTwo iboga-vobasine bisindoles, 16′-decarbomethoxyvoacamine (1) and its 19,20-dihydro derivative, 16′-decarbomethoxydihydrovoacamine (2) from Tabernaemontana corymbosa exhibited potent cytotoxicity against the human colorectal adenocarcinoma HT-29 cells in our previous studies. Bisindoles 1 and 2 selectively inhibited the growth of HT-29 cells without significant cytotoxicity to normal human colon fibroblasts CCD-18Co. Treatment with bisindoles 1 and 2 suppressed the formation of HT-29 colonies via G0/G1 cell cycle arrest and induction of mitochondrial apoptosis. Owing to its higher antiproliferative activity, bisindole 2 was chosen for the subsequent studies. Bisindole 2 inhibited the formation of HT-29 spheroids (tumor-like cell aggregates) in 3D experiments in a dose-dependent manner, while an in vitro tubulin polymerization assay and molecular docking analysis showed that bisindole 2 is a microtubule-stabilizing agent which is predicted to bind at the β-tubulin subunit at the taxol-binding site. The binding resulted in the generation of ROS, which consequently activated the oxidative stress-related cell cycle arrest and apoptotic pathways, viz., JNK/p38, p21Cip1/Chk1, and p21Cip1/Rb/E2F, as shown by microarray profiling.


Synthesis, characterisation and biological activity of diorganotin compounds of (E)-N'-(5-nitro-2-hydroxybenzylidene)-3-hydroxy-2-naphthohydrazide
See Mun Lee, Kong Mun Lo, Li Yuan Liew, Jactty Chew, Chun Hoe Tan, Kae Shin Sim, and Edward R.T. Tiekink
Elsevier BV



Group 6 photo-activable carbon monoxide-releasing molecules (PhotoCORMs) with 1’10-phenanthroline based ligand as potential anti-proliferative and anti-microbial agents
Shiaw Xian Lee, Chun Hoe Tan, Wee Li Mah, Richard Chee Seng Wong, Ninie Suhana Abdul Manan, Yuen Lin Cheow, Kae Shin Sim, and Kong Wai Tan
Elsevier BV



Synthesis, anticancer and antimalarial activities of organosulfur and organoselenium derivatives of cyclopentadienyliron dicarbonyl as photoCORMs
Chun Hau Gan, Kong Wai Tan, Mei Lee Ooi, Jonathan Wee Kent Liew, Yee Ling Ng, Yee Ling Lau, Yin Zhuang Ng, Chew Hee Ng, Chun Hoe Tan, and Richard C.S. Wong
Elsevier BV