Jose Aires Ventura
@es.gov.br
Gerência de Pesquisa e Inovação
Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural - Incaper
His career was devoted principally to diseases of tropical fruit crops. In the last 40 years his research has focused primarily on epidemiology, resistance, host–pathogen interaction, and diseases management on papaya, pineapple and bananas. He was Technical Director from 1995 to 1999 and Head of Research at Incaper from 2000 to 2016. He was honored by SBF in 1995 with the plant pathology award, and in 2006 with the Embrapa Frederico de Meneses Veiga national award for his relevant research with diseases on tropical fruits. Also he was granted the research productivity award from the National Council for Scientific Technological Research (PQ). Dr. Ventura is a member of the Brazilian Phytopathological Society and The American Phytopatological Society (APS).
EDUCATION
He received his M.Sc and Ph.D degrees in Plant Pathology from the Department of Plant Pathology at Federal University of Viçosa, Brazil.
Senior research plant pathologist at Capixaba Institute of Research, Technical Assistance and Rural Extension (Incaper), and Professor of plant biotechnology in the Federal University of Espírito Santo (Ufes) at the Agribusiness Biotechnology Nucleus and Plant Biology, Brazil.
RESEARCH INTERESTS
Research Plant Pathologist
Scopus Publications
Scopus Publications
Nataly Senna Gerhardt Barraqui, José Aires Ventura, Fabiana Gomes Ruas, Rodrigo Borges de Araujo Gomes, Betina Pires Oliveira Lovatti, Samantha Ribeiro Campos da Silva, Paulo Roberto Filgueiras, and Ricardo Machado Kuster
Elsevier BV
Jorge Andrés Duarte, Gabrielle Alves Ribeiro da Silva, Francisco Paiva Machado, Diogo Folly, Evelyn Peñaloza, Rafael Garrett, Marcelo Guerra Santos, José Aires Ventura, Guilherme Freimann Wermelinger, Bruno Kaufmann Robbs,et al.
Elsevier BV
Pablo Viana Oliveira, Alexandre Rosa dos Santos, Emily Lopes Olive, Karolinni Bianchi Britto, Francine Alves Nogueira de Almeida, Vitor Cezar Pacheco da Silva, Carolina Barros Machado, Maurício José Fornazier, José Aires Ventura, Mark Paul Culik,et al.
MDPI AG
Mealybugs are insects belonging to the family Pseudococcidae. This family includes many plant-pest species with similar morphologies, which may lead to errors in mealybug identification and delimitation. In the present study, we employed molecular-species-delimitation approaches based on distance (ASAP) and coalescence (GMYC and mPTP) methods to identify mealybugs collected from coffee and other plant hosts in the states of Espírito Santo, Bahia, Minas Gerais, and Pernambuco, Brazil. We obtained 171 new COI sequences, and 565 from the BOLD Systems database, representing 26 candidate species of Pseudococcidae. The MOTUs estimated were not congruent across different methods (ASAP-25; GMYC-30; mPTP-22). Misidentifications were revealed in the sequences from the BOLD Systems database involving Phenacoccus solani × Ph. solenopsis, Ph. tucumanus × Ph. baccharidis, and Planacoccus citri × Pl. minor species. Ten mealybug species were collected from coffee plants in Espírito Santo. Due to the incorrect labeling of the species sequences, the COI barcode library of the dataset from the database needs to be carefully analyzed to avoid the misidentification of species. The systematics and taxonomy of mealybugs may be improved by integrative taxonomy which may facilitate the integrated pest management of these pests.
Marlonni Maurastoni, Tathiana F. Sá Antunes, Emanuel F. M. Abreu, Simone G. Ribeiro, Angela Mehta, Marcio M. Sanches, Wagner Fontes, Elliot W. Kitajima, Fabiano T. Cruz, Alexandre M. C. Santos,et al.
MDPI AG
Papaya sticky disease is caused by the association of a fusagra-like and an umbra-like virus, named papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), respectively. Both viral genomes are encapsidated in particles formed by the PMeV ORF1 product, which has the potential to encode a protein with 1563 amino acids (aa). However, the structural components of the viral capsid are unknown. To characterize the structural proteins of PMeV and PMeV2, virions were purified from Carica papaya latex. SDS-PAGE analysis of purified virus revealed two major proteins of ~40 kDa and ~55 kDa. Amino-terminal sequencing of the ~55 kDa protein and LC-MS/MS of purified virions indicated that this protein starts at aa 263 of the deduced ORF1 product as a result of either degradation or proteolytic processing. A yeast two-hybrid assay was used to identify Arabidopsis proteins interacting with two PMeV ORF1 product fragments (aa 321–670 and 961–1200). The 50S ribosomal protein L17 (AtRPL17) was identified as potentially associated with modulated translation-related proteins. In plant cells, AtRPL17 co-localized and interacted with the PMeV ORF1 fragments. These findings support the hypothesis that the interaction between PMeV/PMeV2 structural proteins and RPL17 is important for virus–host interactions.
David dos Santos Martins, Maurício José Fornazier, José Aires Ventura, Victor Dias Pirovani, Keiko Uramoto, Rogério Carvalho Guarçoni, Mark Paul Culik, Paulo Sérgio Fiuza Ferreira, and José Cola Zanuncio
Crop Protection Elsevier BV
David dos Santos Martins, Vera Regina dos Santos Wolff, Mark Paul Culik, Beatriz Crisostomo dos Santos, Maurício José Fornazier, and José Aires Ventura
FapUNIFESP (SciELO)
Abstract: Armored scale insects (Hemiptera: Diaspididae), are phytophagous species that occur in major biogeographic regions of the world. Because of the importance of diaspidids as pests, there is widespread interest in countries that export and import unprocessed agricultural products in increased knowledge of this group which includes invasive and quarantine pests of great economic concern. The diversity, geographic distribution, and host of diaspidids were studied from November 2002 to December 2018 in 34 municipalities in the state of Espírito Santo, Brazil. Forty species of Diaspididae from 27 genera were collected and identified. The species Acutaspis perseae (Comstock), A. umbonifera (Newstead), Aonidiella aurantii (Maskell), Comstockaspis perniciosa (Comstock), Lepidosaphes beckii (Newman), Lepidosaphes gloverii (Packard), Morganella longispina (Morgan), Mycetaspis apicata (Newstead), and Thysanofiorinia nephelii (Maskel) were found for the first time in Espírito Santo. The plant families Myrtaceae, Moraceae, Arecaceae, Asparagaceae, and Rutaceae had the greatest number of host plant species of armored scale. Fifty-seven new host associations were observed for 25 species of diaspidids and 11 diaspidid species were recorded for the first time from nine families of plants. Selenaspidus articulatus (Morgan) was the most polyphagous species observed with 17 host plant species from 12 families, followed by Pseudaonidia trilobitiformis (Green), and Parlatoria proteus (Curtis). With these new records, 41 species and 28 genera of Diaspididae have been recorded in Espírito Santo.
Tiago Souza Salles, Marcelo Damião Ferreira Meneses, Lucio Ayres Caldas, Thayane Encarnação Sá-Guimarães, Danielle M. de Oliveira, José A. Ventura, Renata Campos Azevedo, Ricardo M. Kuster, Márcia Regina Soares, and Davis Fernandes Ferreira
Springer Science and Business Media LLC
Abstract Background The arthropod-borne Mayaro virus (MAYV) causes “Mayaro fever,” a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. There are currently no licensed drugs against most mosquito-transmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil have been subjected to phytochemical investigation for the identification and isolation of antiviral compounds. In the present study, we explored the antiviral activity of pomegranate extracts in Vero cells infected with Mayaro virus. Methods The ethanol extract and punicalagin of pomegranate were extracted solely from the shell and purified by chromatographic fractionation, and were chemically identified using spectroscopic techniques. The cytotoxicity of the purified compounds was measured by the dye uptake assay, while their antiviral activity was evaluated by a virus yield inhibition assay. Results Pomegranate ethanol extract (CC50 = 588.9, IC50 = 12.3) and a fraction containing punicalagin as major compound (CC50 = 441.5, IC50 = 28.2) were shown to have antiviral activity (SI 49 and 16, respectively) against Mayaro virus, an alphavirus. Immunofluorescence analysis showed the virucidal effect of pomegranate extract, and transmission electron microscopy (TEM) revealed damage in viral particles treated with this extract. Conclusions The P. granatum extract is a promising source of antiviral compounds against the alphavirus MAYV and represents an excellent candidate for future studies with other enveloped RNA viruses. Graphical abstract
Tiago S. Salles, Marcelo D. F. Meneses, Kristie A. Yamamoto, Thayane E. Sá-Guimarães, Lucio Ayres Caldas, Jessica H. S. Silva, Polianna da Silva Ferreira, Ana Claudia F. Amaral, José A. Ventura, Renata Campos Azevedo,et al.
Springer Science and Business Media LLC
Raissa D. Moura, Lauanda M. Carvalho, Brígida A.A. Spagnol, Tarcio Carneiro, Ane Catarine Tosi Costa, Oeber de F. Quadros, José A. Ventura, Ronaldo S. de Biasi, A. Alberto R. Fernandes, and Patricia M.B. Fernandes
Elsevier BV
Tathiana F. Sá Antunes, Marlonni Maurastoni, L. Johana Madroñero, Gabriela Fuentes, Jorge M. Santamaría, José Aires Ventura, Emanuel F. Abreu, A. Alberto R. Fernandes, and Patricia M. B. Fernandes
Scientific Societies
Among the most serious problems in papaya production are the viruses associated with papaya ringspot and papaya sticky disease (PSD). PSD concerns producers worldwide because its symptoms are extremely aggressive and appear only after flowering. As no resistant cultivar is available, several disease management strategies have been used in affected countries, such as the use of healthy seeds, exclusion of the pathogen, and roguing. In the 1990s, a dsRNA virus, papaya meleira virus (PMeV), was identified in Brazil as the causal agent of PSD. However, in 2016 a second virus, papaya meleira virus 2 (PMeV2), with an ssRNA genome, was also identified in PSD plants. Only PMeV is detected in asymptomatic plants, whereas all symptomatic plants contain both viral RNAs separately packaged in particles formed by the PMeV capsid protein. PSD also affects papaya plants in Mexico, Ecuador, and Australia. PMeV2-like viruses have been identified in the affected plants, but the partner virus(es) in these countries are still unknown. In Brazil, PMeV and PMeV2 reside in laticifers that promote spontaneous latex exudation, resulting in the affected papaya fruit’s sticky appearance. Genes modulated in plants affected by PSD include those involved in reactive oxygen species and salicylic acid signaling, proteasomal degradation, and photosynthesis, which are key plant defenses against PMeV complex infection. However, the complete activation of the defense response is impaired by the expression of negative effectors modulated by the virus. This review presents a summary of the current knowledge of the Carica papaya-PMeV complex interaction and management strategies.
Raíssa Debacker Moura, Luiza Adami Monteiro de Castro, Mark Paul Culik, Antônio Alberto Ribeiro Fernandes, Patricia Machado Bueno Fernandes, and José Aires Ventura
Elsevier BV
Marlonni Maurastoni, Tathiana F. Sá-Antunes, Scarlett A. Oliveira, Alexandre M. C. Santos, José A. Ventura, and Patricia M. B. Fernandes
Springer Science and Business Media LLC
Bruno G. Oliveira, Elisangela F. Pimentel, Ana C. Hertel Pereira, Flávia Tosato, Fernanda E. Pinto, José A. Ventura, Denise C. Endringer, and Wanderson Romão
Elsevier BV
José G.M. Esgario, Renato A. Krohling, and José A. Ventura
Elsevier BV
Dayane Littig Barker, Sara Dousseau Arantes, Edilson Romais Schmildt, José Aires Ventura, Lúcio de Oliveira Arantes, Jasmini Fonseca da Silva, Basílio Cerri Neto, Stanley Bravo Buffon, and Patrícia Soares Furno Fontes
FapUNIFESP (SciELO)
Abstract: The objective of this work was to evaluate the effect of shoot types and plant ages for floral induction on the performance of 'Vitória' pineapple (Ananas comosus). The experiment was carried out from April 2015 to December 2016, using shoots of two different classifications (slips of 100 to 200 g and suckers of 201 to 300 g). Artificial floral induction was performed at the eighth, tenth, and twelfth months after planting, and natural induction was also evaluated. Evaluations for vegetative development, phenology, and productivity were performed. A significant interaction was observed between the studied factors for width and area of the “D” leaf. Shoot type did not influence productivity. Natural flowering extended the crop cycle by 617 days. The induction performed at the eighth month anticipated harvest by up to 167 days. Earlier inductions reduced productivity by 58.15% due to the reduction of fruit mass. Naturally induced plants produced larger fruit ranging from 1.0 to 1.2 kg. Inductions from the eighth to the tenth month promote harvesting in more favorable seasons.
Sarah C. Maximino, Jessyca A.P. Dutra, Ricardo P. Rodrigues, Rita C.R. Gonçalves, Pedro A.B. Morais, José A. Ventura, Ricardo P. Schuenck, Valdemar Lacerda Júnior, Rodrigo R. Kitagawa, and Warley S. Borges
Bentham Science Publishers Ltd.
Background: Fusarium solani f. sp. piperis is a phytopathogen that causes one of the most destructive diseases in black pepper crops, resulting in significant economic and crop production losses. Consequently, the control of this fungal disease is a matter of current and relevant interest in agriculture. Objective: The objective was to synthesize eugenol derivatives with antifungal activity. Methods: In this study, using bimolecular nucleophilic substitution and click chemistry approaches, four new and three known eugenol derivatives were obtained. The eugenol derivatives were characterized and their antifungal and cytotoxic effects were evaluated. Results: Eugenol derivative 4 (2-(4-allyl-2-methoxyphenoxy)-3-chloronaphthalene-1,4-dione) was the most active against F. solani f. sp. piperis and showed acceptable cytotoxicity. Compound 4 was two-fold more effective than tebuconazole in an antifungal assay and presented similar cytotoxicity in macrophages. The in silico study of β-glucosidase suggests a potential interaction of 4 with amino acid residues by a cation-π interaction with residue Arg177 followed by a hydrogen bond with Glu596, indicating an important role in the interactions with 4, justifying the antifungal action of this compound. In addition, the cytotoxicity after metabolism was evaluated as a mimic assay with the S9 fraction in HepG2 cells. Compound 4 demonstrated maintenance of cytotoxicity, showing IC50 values of 11.18 ± 0.5 and 9.04 ± 0.2 μg mL-1 without and with the S9 fraction, respectively. In contrast, eugenol (257.9 ± 0.4 and 133.5 ± 0.8 μg mL-1), tebuconazole (34.94 ± 0.2 and 26.76 ± 0.17 μg mL-1) and especially carbendazim (251.0 ± 0.30 and 34.7 ± 0.10 μg mL-1) showed greater cytotoxicity after hepatic biotransformation. Conclusion: The results suggest that 4 is a potential candidate for use in the design of new and effective compounds that could control this pathogen.
Rodrigo Borges de Araujo Gomes, Erica Santana de Souza, Nataly Senna Gerhardt Barraqui, Cristina Luz Tosta, Ana Paula Ferreira Nunes, Ricardo Pinto Schuenck, Fabiana Gomes Ruas, José Aires Ventura, Paulo Roberto Filgueiras, and Ricardo Machado Kuster
Elsevier BV
Bruno G. Oliveira, Flavia Tosato, Gabriely S. Folli, Júlia A. de Leite, José A. Ventura, Denise C. Endringer, Paulo R. Filgueiras, and Wanderson Romão
Elsevier BV
Maria E.S. Barroso, Bruno G. Oliveira, Elisângela F. Pimentel, Pedro M. Pereira, Fabiana G. Ruas, Tadeu U. Andrade, Dominik Lenz, Rodrigo Scherer, Marcio Fronza, José A. Ventura,et al.
Elsevier BV
Lorena Carnielli-Queiroz, Patricia Machado Bueno Fernandes, Antônio Alberto Ribeiro Fernandes, and José Aires Ventura
FapUNIFESP (SciELO)
Pineapple (Ananas comosus var. comosus) fusariosis is an economically important fungal disease affecting the plant and its fruit. A rapid and reliable diagnosis is the base of integrated disease management practices. Fusariosis has resulted in quarantines for pineapple products in Central America, Africa and Asia. Difficulties diagnosing and correctly identifying the fungus Fusarium guttiforme, agent of the pineapple fusariosis, have led to the search for new methodologies, and for this we developed a new reliable molecular method to detect it. For diagnostic purposes, real-time PCR of elongation factor gene 1-α (ef1) was used to rapidly, specifically and sensitively diagnose F. guttiforme. A pathogenicity HIGHLIGHTS • A rapid and reliable diagnosis of Fusarium guttiforme in pure cultures and infected pineapple; • F. guttiforme could be easily distinguished from other Fusarium species by real-time PCR; • A molecular method provide support and can be helpful for effective disease management; • New method for indexation of pineapple propagative material for phytosanitary defence. 2 Carnielli-Queiroz, L.; et al. Brazilian Archives of Biology and Technology. Vol.62: e19180591, 2019 www.scielo.br/babt test was conducted with slips of the pineapple cultivar Pérola, a multiplex PCR was run, and the results compared with those obtained with real-time PCR. The real-time PCR assay with its specific primer set could readity distinguish F. guttiforme from other Fusarium species known to occur on pineapple. The real-time PCR test had 95% sensitivity and 100% specificity with a significance level p<0.0001. For field samples the test had 100% sensitivity and specificity. Thus, this new test is fit for use in serial analyses of pineapples, and may have application in the evaluation of propagation materials and making quarantine decisions. The ability to rapidly and specifically detect F. guttiforme in plant samples will facilitate monitoring of the pathogen and improve disease management.
José Aires Ventura, Inorbert de Melo Lima, Marlon Vagner Valentim Martins, Mark Paul Culik, and Hélcio Costa
FapUNIFESP (SciELO)
Abstract Well-formed and healthy propagative material is essential for economic success in fruit growing. The health of the seedlings must be ensured during production to prevent the death of plants and spread of pathogens to new areas, which cause an increase in production costs and reduction in yield, with some pathogens making production unviable in the areas where they are introduced. The most viable strategy for disease control in propagative material is the use of resistant cultivars. However, for many fruit cultivars sources of resistance have not yet been identified and in some cases the resistance is “broken” by the emergence of new races of the pathogen. Other measures are also important and recommended in plant propagation, such as the use of integrated disease management, the use of cultural and biological methods, substrate preparation, irrigation management, grafting, balanced nutrition and use of organic matter, eliminating the initial inoculum, and reducing the disease rate. Among the various procedures in nurseries for ensuring the health of seedlings are the use of pathogen-free seeds and cuttings, handling of substrates in clean facilities, disinfection of hands, tools and containers, maintenance of water quality for irrigation, and the elimination of invasive plants. It is important to clean the nurseries and have a suitable place for the disposal of seedlings, substrates and crop residues. A record and history of the operations in the production of seedlings should be a routine, as well as security and control in access to greenhouses or nurseries. The application of knowledge and the best strategies of integrated disease management for the production of healthy seedlings, guarantees the quality of the productive material and the success of the crop.
F.N. Peron, R. Calloni, J.A. Ventura, and P.M.B. Fernandes
International Society for Horticultural Science (ISHS)
Mealybug wilt of pineapple (MWP) is a disease caused by the Pineapple mealybug wilt-associated virus (PMWaV) complex transmitted by Dysmicoccus brevipes and D. neobrevipes. MWP symptoms are characterized by root dessication, leaf wilting and consequent failure to produce a fruit. The molecular mechanisms involved in the pineapple-PMWaV interaction for MWP symptomatology are still unclear. In this work, mRNAs of asymptomatic and symptomatic pineapple plants were evaluated using Illumina RNA sequencing technology. From a total of 79 million reads per sample, 16,097 genes were identified using STAR aligner and HTseq for paired-end files. Differentially expressed genes (DEGs) between the evaluated groups were estimated using DESeq2 and edgeR, with an FDR cutoff of ≤0.05. A total of 207 DEGs were detected, with 61 upregulated and 146 downregulated in symptomatic plants infected by PMWaV-2. The methodologies improved by the assays presented in this article and the detected DEGs can substantiate further researches with pineapple and the MWP disease.