Muhammad Ahsan Naeem

@uvas.edu.pk

Lecturer, Department of Basic Sciences (Pharmacology), KBCMA College of Veterinary and Animal Sciences, Narowal
University of Veterinary and Animal Sciences, Lahore, Pakistan

Muhammad Ahsan Naeem


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https://researchid.co/muhammad143
Personal
Date of Birth: June27, 1988
Gender: Male
Marital Status: Married
Permanent Address: Faisalabad, Pakistan
Contact #: +923036382900
E. Mail: muhammadahsannaeem@
Current Designation
1. Lecturer (Pharmacology), KBCMA College of Veterinary and Animal Sciences, Narowal (SubCampus University of Veterinary and Animal Sciences, Lahore) Pakistan.
2. Higher Education Commission (HEC), Pakistan approved PhD Supervisor in the field of Pharmacology.
3. Associate Fellow, Higher Education Academy (AFHEA), UK Professional Standard Framework (Fellowship Reference: PR240695).

EDUCATION

May31-Jun11, 2021 Institut de Pasteur, Paris, France Diploma (Tuberculosis)
Sept 2016- Jun 2020 Huazhong Agricultural University, Wuhan, China PhD (Preventive Veterinary Medicine)
Sept 2012-May 2015 University of Agriculture, Faisalabad, Pakistan M. Phil. (Pharmacology)
Sept 2007-Jul 2012 University of Agriculture, Faisalabad, Pakistan Doctor of Veterinary Medicine (DVM)

RESEARCH INTERESTS

Pharmacokinetics, Pharmacology, Molecular Biology, Genetics

FUTURE PROJECTS

Value added blue-green algae: A promising alternative/complement feed for broilers (My role in this project is as a main team member)


Applications Invited
Student

Development of herbal formulation against poultry pathogens as an alternative to antibiotic (My role in this project is as a main team member)


Applications Invited
Student

Rapid Foot and Mouth Disease (FMD) antigen detection kit development as a point of care (POC) strategy (My role in this project is as a main team member)


Applications Invited
Student
49

Scopus Publications

Scopus Publications

  • The virulence regulator Rv3425 mediates stress adaptation and promotes intracellular survival of Mycobacterium tuberculosis avirulent strain
    Wei Zhu, Aikebaier Reheman, Guanghui Dang, Yifan Wang, Huaiyuan Cai, et al.
    Archives of Microbiology, 2026
  • Susceptibility of Rhipicehalus (Boophilus) microplus (Acari: Ixodidae) to sulfur and copper nanoparticles
    Sahar Younis, Muhammad Sohail Sajid, Muhammad Ahsan Naeem, Hafiz Muhammad Rizwan, Tanzila Sahar, et al.
    Medical and Veterinary Entomology, 2026
    In District Faisalabad, ticks, such as Hyalomma (H) anatolicum Koch and Rhipicephalus ( Boophilus ) microplus Canestrini (Ixodida: Ixodidae), are significant ectoparasites impacting livestock and wildlife, transmitting pathogens that lead to substantial economic losses. The increasing resistance of tick populations to conventional acaricides necessitates te exploration of potential complementary approaches as control strategies, including nanoparticle formulations. The acaricidal effect of sulfur (S) and copper (Cu) nanoparticles was evaluated against various life stages (eggs, larvae, and adults) of R. (B.) microplus . Nanoparticles were synthesized and characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The susceptibility of ticks to nanoparticles was evaluated by adult and larval immersion tests and larval hatch tests. Ivermectin was used as a positive control. The lethal concentration to 50% mortality (LC 50 ) values for Cu and S nanoparticles against adult R. (B.) microplus were 22.3 ± 3.44 mg/L and 36.16 ± 6.19 mg/L, respectively. At higher concentrations of 80 mg/L (Cu) and 150 mg/L (S), both nanoparticles achieved 99.17% adult tick mortality. S and Cu nanoparticles demonstrated 99.87% and 98.50% larval mortality at concentrations of 150 mg/L and 80 mg/L, respectively. Lower mortality was observed with Ivermectin at 30 mg/L, with 66.67% adult mortality and 61.50% larval mortality. Cu and S also resulted in 99.87% unviable eggs at concentrations of 80 mg/L and 150 mg/L, respectively, while Ivermectin led to only 90.63% unviable eggs. S and Cu nanoparticles exhibited significant potential as effective complementary agents to traditional acaricides, disrupting the life cycle of R. (B.) microplus . Further research is essential to assess their long‐term ecological impacts and safety for non‐target organisms.
  • COVID-19 Pandemic: A Comprehensive Meta-Review of Global Impacts, Responses, and Future Preparedness
    Rulin Wang, Muhammad Ahsan Naeem
    Clinical Respiratory Journal, 2025
    Introduction The COVID‐19 pandemic due to SARS‐CoV‐2 has initiated historically unparalleled global health, social, and economic impacts. Syntheses of the multivariable interdependent effects on the multiple clinical, immunologic, psychosocial, and health service realms are required to guide current and future public health preparedness and policy. Methods A systematic review of a meta‐analysis was conducted using the PubMed, Scopus, and Web of Science databases from December 2019 to 2025 to identify observational studies and randomized controlled trials. Quantitative reporting studies of COVID‐19 outcomes were included. Random‐effects model aggregated effect sizes were estimated and tested for heterogeneity with Cochran's Q, τ 2 , and I 2 . Subgroup, moderator, publication bias, sensitivity, and leave‐one‐out analyses were conducted for exploration and validation. Results Twenty‐four studies were classified into three categories: clinical outcomes (15 studies), immunogenicity (4 studies), and psychosocial/health service outcomes (5 studies). There was no statistically significant pooled effect (effect ratio 0.95, 95% CI: 0.55–1.62) with severe heterogeneity ( I 2 > 99%). Immunogenicity showed a statistically significant positive effect (pooled estimate 0.77, 95% CI: 0.38–1.16) with high heterogeneity (I 2 ~96%). Psychosocial effects were highly heterogeneous with non‐significant overall effects (estimate −1.03, 95% CI: −5.74 to 3.69). Sample size was an influential moderator that explained significant between‐group heterogeneity. Discussion The outcomes reveal robust immunogenic vaccine impacts and indeterminate psychosocial and clinical impacts, consistent with the heterogeneity and complexity of COVID‐19 effects. Great heterogeneity highlights methodological standardization and cautious interpretation. This present meta‐analysis offers key lessons to guide subsequent study design and manufacture of fair health policy and pandemic readiness.
  • Optimizing a Subunit Vaccine of Mycobacterium tuberculosis Using In-Silico and In-Vitro Approaches
    Zaiqin Ling, Muhammad Ahsan Naeem
    Iranian Journal of Public Health, 2025
    Background: The present study addresses the development of a novel subunit vaccine (SV) to combat tuberculosis (TB).Methods: The research used immunoinformatics to develop a subunit vaccine with 7 MHC-I, 3 MHC-II, and 7 B-cell epitopes joined by AAV, GPGPG, and KK linkers. It involved Mtb protein Rv0577 and PADRE sequence as an adjuvant. TLR2 binding affinity (Kd, nM) was determined through PRODIGY. In-silico evaluations determined allergenicity, antigenicity, and physicochemical properties. The vaccine was presented in an AAVDj/8 system, intracellular expression was verified, and the copy number was identified using qPCR and qRT-PCR.Results: The web tools confirmed the stability, non-allergenicity, and high immunogenicity of the vaccine (0.5673 < 0.4). PRODIGY tool depicted good SV-TLR2 binding (ΔG = -8.8 kcal/mol, Kd = 330 nM) with 59 intermolecular contacts, indicating possible TLR2 activation. Indirect immunofluorescence showed the expression of intracellular proteins. Viral titers, determined by 10-fold serial dilution up to 10³, showed a detectable titer, and copy numbers (10⁹/mL–10¹¹/mL) proved productive viral replication and significant vaccine effectiveness.Conclusion: This comprehensive methodology, from epitope selection to in-vitro testing, establishes a robust foundation for further exploring and advancing this SV.
  • IMMUNOINFORMATICS-DRIVEN MULTI-EPITOPE VACCINE DESIGN FOR FOOT-AND-MOUTH DISEASE VIRUS
    A Alamri, S Imran, M.A. Abdel-Maksoud, S Alumtairi, H Ebaid, et al.
    Journal of Animal and Plant Sciences, 2025
    Foot-and-mouth disease (FMD) is the most destructive for livestock with reservoirs in cattle, buffalo, sheep, goats, and pigs and foot-and-mouth disease virus (FMDV) with seven serotypes. Current vaccination strategies suffer from difficulties with antigenic variability coupled with high costs. This paper aims to design a multi-epitope subunit vaccine against FMDV by the use of immunoinformatics approach in order to improve effectiveness. We used immunoinformatics to design a subunit vaccine that included two T-cell epitopes linked with AAV and three B-cell epitopes linked with KK. T- and B-cell epitopes were joined by a GPSL linker. A Pan HLA-DR binding epitope, PADRE, was attached at both ends using EAAAK linkers. Physicochemical properties, allergenicity, and antigenicity of the vaccine were evaluated, along with secondary and tertiary structure predictions and molecular docking studies with the Toll-like receptor 9 (TLR-9). The vaccine had a predicted to be non-allergic and with high antigenic property (0.73). Physicochemical analysis showed to be 135 amino acids, stable (21.29 stability index), and basic (pI of 10.51). The overall 3D structure showed robust binding affinity against the cattle TLR-9 receptor. It was confirmed, in silico cloning, effective transformation into prokaryotic expression vector pET-28a (+). The subunit vaccine developed based on immunoinformatics has great promise in the form of a high antigenicity level, stabilized physicochemical properties, and interactions well-favored with TLR-9. This indicates potential further experimental validation of effectiveness as a candidate vaccine against FMD. Keywords: FMDV, subunit vaccine, immunoinformatics, non-structural protein
  • Comparative Transcriptional Analysis of Long Noncoding RNAs in Oxidative Stress and Inflammation Induced by Potassium Permanganate and Lipopolysaccharide in Rat Uterine Tissues
    Talha Umar, Huili Feng, Wen Feng, Han Zhou, Nuoer Chen, et al.
    Antioxidants, 2025
    Potassium permanganate (KMnO4) is a commercially available antiseptic used in bovine intrauterine lavage to manage postpartum infections. Lipopolysaccharides (LPS) are well-studied for their ability to induce inflammation and oxidative stress. While KMnO4 is known to cause significant irritation, oxidative stress, and toxicity in uterine tissues, its transcriptional impact and potential for inducing similar molecular damage as LPS have not been fully explored. In this study, we induced oxidative stress in the uterine tissues of Sprague–Dawley (SD) rats using KMnO4 and compared the transcriptional profiles with those treated with LPS. We focused on the differential expression of long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) related to oxidative stress, toxicity, and inflammation. RNA sequencing revealed 1125 differentially expressed mRNAs in the KMnO4-treated group and 989 in the LPS-treated group. Additionally, 1649 lncRNAs were differentially expressed in the KMnO4 group compared with 1383 in the LPS group. Gene ontology (GO) and KEGG enrichment analyses showed that 78 pathways were significantly enriched in the KMnO4 group, while 80 pathways were enriched in the LPS group, with 50 pathways shared between the two. This study offers critical insights into the transcriptional profiles associated with KMnO4 exposure and its similarities to LPS-induced damage.
  • Pharmacological role of bioactive compounds of date palm by-products
    Sidra Altaf, Tasawar Iqbal, Muhammad Ahsan Naeem, Maimoona Ikram
    Sustainable Valorization of Date Palm by Products and Wastes, 2025
  • Protective Effects of Olive Leaf Extract as a Natural Growth Promoter and Immune Modulator in Broilers Challenged with Velogenic Newcastle Disease Virus
    MZ Shakir, M Usman, M Imran, MA Naeem, F Rizvi, et al.
    Revista Brasileira De Ciencia Avicola Brazilian Journal of Poultry Science, 2025
    Newcastle disease (ND) is a serious menace to poultry production and requires stringent measures to enhance growth and improve resistance against viral challenges in infected flocks. In the present study, the immunostimulatory and growth-promoting effects of olive (Olea europaea) leaf extract (OLE) in velogenic Newcastle disease virus (NDV)-infected broilers were evaluated. A total of 150-day-old broilers (Cobb strain) were randomly allotted to five groups: Blank Control (BC), Negative Control (NC), OLE-1, OLE-2, and OLE-Only. OLE-1 and OLE-2 groups were given 10 mL and 20 mL/L of OLE-supplemented drinking water on day 3, and ND vaccinations on days 0, 3, and 14. The NC, OLE-1, and OLE-2 groups were given an intraperitoneal challenge of NDV on day 21. Weekly inspections comprised clinical signs, survival, antibody levels, weight gain, and hematological indices. Body weight gain and leukocyte number (p<0.05) were significantly enhanced with OLE supplementation, whereas mortality was lowered to 30%. Proventriculus hemorrhage and intestinal ulcers were more severe in the NC group but less severe in the OLE-treated groups. OLE also attenuated ND-caused tissue injury and increased erythrocyte number, hemoglobin content, and packed cell volume. In conclusion, OLE boosts broiler immunity and growth performance without causing any unfavorable physiological effects, hence being a possible natural additive to improve disease resistance and poultry production.
  • Amelioration of Organophosphate Poisoning Using Red Blood Cell Membrane-Cloaked Oil Nano-Sponge
    Journal of Biological Regulators and Homeostatic Agents, 2024
  • Antiparasitic drug resistance in veterinary practice
    Antiparasitic Drug Resistance in Veterinary Practice, 2024
  • Phenotypic methods for determining antiparasitic resistance in vitro and in vivo
    HazratUllah Raheemi, Zobia Afsheen, Muhammad Ahsan Naeem, Shamshad Fareed, Xi Chen, et al.
    Antiparasitic Drug Resistance in Veterinary Practice, 2024
  • Role of nanoparticles and nanotherapeutics in the diagnosis of serious zoonotic and neurological diseases
    Nida Wazir, Maria Asghar, Sahar Younis, Muhammad Ahsan Naeem, Waqas Ahmad, et al.
    Theranostic Applications of Nanotechnology in Neurological Disorders, 2024
  • Evaluation of influence of Butea monosperma floral extract on inflammatory biomarkers
    Nureen Zahra, Aansa Mazhar, Beenish Zahid, Muhammad Ahsan Naeem, Abid Sarwar, et al.
    Open Chemistry, 2024
  • A Review on the Applications of Potassium Permanganate in Veterinary Medicine: Toxicity, Efficacy and Future Considerations
    Pakistan Veterinary Journal, 2024
  • In vitro and in vivo anthelmintic response of the seeds of Amomum subulatum roxb and Vitex negundo
    S. N. Badar, M. S. Sajid, H. M. Rizwan, H. Abbas, M. Maqbool, et al.
    Brazilian Journal of Biology, 2024
  • Involvement of 2′-5′ oligoadenylate synthetase-like protein in the survival of Mycobacterium tuberculosis avirulent strain in macrophages
    Aikebaier Reheman, Xiaojian Cao, Yifan Wang, Xi Nie, Gang Cao, et al.
    Animal Diseases, 2023
  • Evaluation of trace elements in forages and their effect on gastrointestinal parasite burden in grazing sheep
    Hafiz Muhammad Rizwan, Muhammad Sohail Sajid, Muhammad Younus, Muhammad Ahsan Naeem, Muhammad Sulman Ali Taseer, et al.
    Saudi Journal of Biological Sciences, 2023
  • Seasonal changes in the scrotal skin histology in relation to thermoregulation and testosterone level in camel and buffalo bull
    Shakil Ahmed, Anas Sarwar Qureshi, Muhammad Usman, Sarmad Rehan, Farrah Deeba, et al.
    Journal of Veterinary Medicine Series C Anatomia Histologia Embryologia, 2023
  • Impact of artificial intelligence and communication tools in veterinary and medical sciences: Ai in health sciences
    Muhammad Rizwan Saeed, Muhammad Abdullah, Muhammad Zoraiz, Waqas Ahmad, Muhammad Ahsan Naeem, et al.
    AI and Its Convergence with Communication Technologies, 2023
  • Parasite control strategies: Trace elements and minerals
    Haider Abbas, Hafiz Muhammad Rizwan, Muhammad Younus, Muhammad Umar Farid, Muhammad Ahsan Naeem, et al.
    Parasitism and Parasitic Control in Animals Strategies for the Developing World, 2023
  • Pathology of parasitic infections
    Tayyaba Akhtar, Muhammad Usman Amanat, Nida Wazir, Muhammad Ifham Naeem, Muhammad Ammar, et al.
    Parasitism and Parasitic Control in Animals Strategies for the Developing World, 2023
  • Histo-biochemical evaluation of spearmint dried leaves extract on selected organs of pregnant albino rats and their neonates: A long-term study
    Pakistan Journal of Pharmaceutical Sciences, 2023
  • Age, sex, and chilling effects on goat meat
    Muhammad Umar Farid, Muhammad Hayat Jaspal, Nida Luqman, Bilal Asghar, Muawuz Ijaz, et al.
    Italian Journal of Food Science, 2023
  • Effects of Saccharomyces Cerevisiae Supplemented Diet on Production Performance, Egg Quality and Humoral Immunity in Black Australorp and Fayoumi Layers
    Revista Brasileira De Ciencia Avicola Brazilian Journal of Poultry Science, 2023
  • Prevalence of Black Quarter in Association with Patho-Morphological Alterations in Affected Tissues at Different Zones of Punjab-Pakistan
    Advancements in Life Sciences, 2022

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)

In my PhD, I have designed sub-unit vaccine against human tuberculosis by applying immunoinformatics approach using its pathogen's secretory proteins. pre-clinical trials were performed in mice model and obtained successful results. Now I along with my PhD supervisor from China are in a plan to patent it.

Industry, Institute, or Organisation Collaboration

I am currently in collaboration with my lab from where I have done my PhD from Huazhong Agricultural University, Wuhan, China in a book preparation.

INDUSTRY EXPERIENCE

I have a basic working knowledge of pharmaceutical industry.