Muhammad Ahsan Naeem
@uvas.edu.pk
Lecturer, Department of Basic Sciences (Pharmacology), KBCMA College of Veterinary and Animal Sciences, Narowal
University of Veterinary and Animal Sciences, Lahore, Pakistan
Personal
Date of Birth: June27, 1988
Gender: Male
Marital Status: Married
Permanent Address: Faisalabad, Pakistan
Contact #: +923036382900
E. Mail: muhammadahsannaeem@
Current Designation
1. Lecturer (Pharmacology), KBCMA College of Veterinary and Animal Sciences, Narowal (SubCampus University of Veterinary and Animal Sciences, Lahore) Pakistan.
2. Higher Education Commission (HEC), Pakistan approved PhD Supervisor in the field of Pharmacology.
3. Associate Fellow, Higher Education Academy (AFHEA), UK Professional Standard Framework (Fellowship Reference: PR240695).
EDUCATION
May31-Jun11, 2021 Institut de Pasteur, Paris, France Diploma (Tuberculosis)
Sept 2016- Jun 2020 Huazhong Agricultural University, Wuhan, China PhD (Preventive Veterinary Medicine)
Sept 2012-May 2015 University of Agriculture, Faisalabad, Pakistan M. Phil. (Pharmacology)
Sept 2007-Jul 2012 University of Agriculture, Faisalabad, Pakistan Doctor of Veterinary Medicine (DVM)
RESEARCH INTERESTS
Pharmacokinetics, Pharmacology, Molecular Biology, Genetics
FUTURE PROJECTS
Value added blue-green algae: A promising alternative/complement feed for broilers (My role in this project is as a main team member)
Applications Invited
Student
Development of herbal formulation against poultry pathogens as an alternative to antibiotic (My role in this project is as a main team member)
Applications Invited
Student
Rapid Foot and Mouth Disease (FMD) antigen detection kit development as a point of care (POC) strategy (My role in this project is as a main team member)
Applications Invited
Student
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
S. N. Badar, M. S. Sajid, H. M. Rizwan, H. Abbas, M. Maqbool, M. A. Malik, R. H. A. Nisar, M. Rajput, A. Shamim, Q. Akram,et al.
FapUNIFESP (SciELO)
Abstract The current study was designed to check the anthelmintic activities of some local plants. Seeds of Amomum (A.) subulatum and Vitex (V.) negundo in different solvents were subjected to in vitro (adult motility assay; AMA and egg hatch assay; EHA) and in vivo (faecal egg count reduction test; FECRT) anthelmintic activity testing protocols using Haemonchus (H.) contortus as an experimental model. The results of AMA, EHA, and FECRT were statistically analysed through linear regression and Duncan multiple range test. In AMA test, at 50 mg mL-1 concentration, the percent mortality of H. contortus was higher in A. subulatum than V. negundo, whereas, in EHA test, A. subulatum was proven better ovicidal (LC50=14.2 µg mL-1) than V. negundo (LC50= 65.7405 µg mL-1). The FECRT also indicated the better efficacy of A. subulatum than V. negundo against natural infection of gastrointestinal (GI) parasites. The crude powder of plants used in this study showed 29.6% to 57.7% anthelmintic. The reduction rate was found higher for A. subulatum (3 g kg-1) as compared to V. negundo (7 g kg-1). Reagrding efficacy analysis of solvents used for plants extract, ethyl acetate and chloroform were found better in increasing ovicidal activity in adult worms (in vitro testing), whereas, the crude aqueous methanol was found better than the crude powders in in vivo testing. It will be beneficial to document the indigenous knowledge to standard scientific procedures for their validation. This study will help to motivate the farmers to make a better choice of cultivation of the indigenous plants because of their varying efficacies as an alternative preventive approach against the GI parasitic infections.
Aikebaier Reheman, Xiaojian Cao, Yifan Wang, Xi Nie, Gang Cao, Wei Zhou, Bing Yang, Yingying Lei, Weipan Zhang, Muhammad Ahsan Naeem,et al.
Springer Science and Business Media LLC
AbstractMycobacterium tuberculosis (M. tuberculosis) can replicate in the macrophage by interfering with many host protein functions. While it is far from known these host proteins for controlling M. tuberculosis infection. Herein, we infected macrophages including THP-1 and Raw264.7 cells with M. tuberculosis and identified the differentially expressed genes (DEGs) in the interferon signaling pathway. Among them, 2′-5′ oligoadenylate synthetase-like (OASL) underwent the greatest upregulation in M. tuberculosis-infected macrophages. Knockdown of the expression of OASL attenuated M. tuberculosis survival in macrophages. Further, bioinformatics analysis revealed the potential interaction axis of OASL-TAB3- Rv0127, which was further validated by the yeast-two-hybrid (Y2H) assay and Co-IP. This interaction axis might regulate the M. tuberculosis survival and proliferation in macrophages. The study reveals a possible role of OASL during M. tuberculosis infection as a target to control its propagation.
Hafiz Muhammad Rizwan, Muhammad Sohail Sajid, Muhammad Younus, Muhammad Ahsan Naeem, Muhammad Sulman Ali Taseer, and Hossam Ebaid
Elsevier BV
Shakil Ahmed, Anas Sarwar Qureshi, Muhammad Usman, Sarmad Rehan, Farrah Deeba, Muhammad Khalil Ateeq, Muhammad Younus, Muhammad Ahsan Naeem, Zaima Umar, and Muhammad Sulman Ali Taseer
Wiley
This study was conducted to compare the scrotal skin dynamics of camel and buffalo bulls regarding thermoregulation, season and testosterone. Scrotal skin and blood samples (n = 8 each) were collected during breeding (October to March) and non-breeding seasons (April to August). Tissue slides were prepared by the paraffin embedding technique followed by hematoxylin and eosin (H&E) staining and were analysed by ImageJ®. Relative humidity and temperature were recorded in different seasons to calculate the thermal humidity index (THI). Serum testosterone level was estimated through radioimmunoassay (RIA). The data were analysed by applying ANOVA, the THS test was used as a post-ANOVA interface and the correlation coefficient was also calculated among season, testosterone and skin parameters. The results showed that skin thickness, season and THI were negatively correlated with testosterone levels. In the neck, body and apex, the papillary layer was thicker in buffalo bull compared to camel bull during the breeding season; however, the reticular layer followed a reverse trend in the apex when THI was low. In the scrotal neck during the non-breeding season, hair follicle (HF) length and density were recorded high in camel bull compared to buffalo bull when THI was elevated. The density of HF followed the otherwise trend in the apex during the breeding season. The density of SG was found highest in all regions of camel bull as compared to buffalo bull and showed direct relation with THI. To conclude, this study delineates how the histo-dynamics of scrotal skin varies and accommodate itself with testosterone and THI over the different season in camel and buffalo.
Muhammad Rizwan Saeed, Muhammad Abdullah, Muhammad Zoraiz, Waqas Ahmad, Muhammad Ahsan Naeem, Qaiser Akram, and Muhammad Younus
IGI Global
Artificial intelligence (AI) has revolutionized the scientific and biological fields to facilitate animal and human health. The purpose of this chapter is to explain the impact and role of AI in medical health, animal health, and the coordination of these health sectors using information and communication tools (ICTs) to facilitate health for all in general. Precision medicine, drug discovery, robotics, machine learning, and language processing through AI have been well-elaborated and integrated. AI replaced the major human workforce and also reduced the use of excessive manpower, biologicals, machines, radiations, and surgical instruments to analyze, investigate, and make tentative diagnoses not only in humans but also in various fields of veterinary sciences. In this chapter, major achievements and highlights made by AI technologies and ICTs were described comprehensively from different databases.
Haider Abbas, Hafiz Muhammad Rizwan, Muhammad Younus, Muhammad Umar Farid, Muhammad Ahsan Naeem, Muhammad Sulman Ali Taseer, Maria Asghar, Neil Sargison, and Maxwell Opara
CABI
Tayyaba Akhtar, Muhammad Usman Amanat, Nida Wazir, Muhammad Ifham Naeem, Muhammad Ammar, Muhammad Ahsan Naeem, Asif Idrees, Waqas Ahmad, Rohit Tyagi, and Vanessa Slack
CABI
Muhammad Umar Farid, Muhammad Hayat Jaspal, Nida Luqman, Bilal Asghar, Muawuz Ijaz, Muhammad Kashif Yar, Muhammad Sulman Ali Taseer, Zeeshan Iqbal, Muhammad Ahsan Naeem, and Jamal Nasir
Codon Publications
Production and processing factors that affect native goat meat quality are non-existent in the literature. The current study explored age, sex, and chilling effects on 48 goat carcasses cut in half by halal standards and shifting one half immediately to a walk-in chiller at 0–4°C [rapid chilling (RC)] and the other half at 26 ± 2°C [delayed chilling (DC)]. Results showed that Warner Bratzler Shear Force (WBSF) was significantly affected by RC. Cooking loss and color (L*) parameters were significantly higher in young male animals. Chroma values were significantly higher in DC. In conclusion, DC enhances meat quality and tenderness.
Kiran Idrees, Abida Aziz, Muhammad Naeem, Muhammad Farooq Azhar, Shahid Farooq, and Mubshar Hussain
Springer Science and Business Media LLC
Khaista Rahman, Muhammad Jamal, Xi Chen, Wei Zhou, Bin Yang, Yanyan Zou, Weize Xu, Yingying Lei, Chengchao Wu, Xiaojian Cao,et al.
Elsevier BV
H. M. Rizwan, M. Usman, M. A. Naeem, M. U. Farid, M. Younus, M. S. Sajid, U. B. Tahir, N. Luqman, H. Abbas, M. K. Ateeq,et al.
Walter de Gruyter GmbH
Summary The present study reports the prevalence of Paramphistomum spp. in small and large ruminants and their association with the histopathology of the infected rumens. A total of 384 animals were screened for Paramphistomum spp. The animals found positive for Paramphistomum spp. were divided into three groups according to the worm load/5 cm2 (G1: 10 – 20 worms/5 cm2 = Low, G2: 20 – 40 worms/5 cm2 = Medium, and G3: >41 worms/5 cm2 = High). Tissue slides were prepared from samples of the rumen (1 cm2) taken from animals positive for ruminal fluke to determine the histological parameters, including epithelial length or thickness, length and width of the ruminal papilla, and thickness of tunica submucosa and mucularis externae. The overall prevalence of Paramphistomum spp. in the ruminant population of district Narowal was 56.25 % with a significant (P < 0.05) variation among different species of ruminants. The highest prevalence was in cattle, followed in order by buffalo, goat, and sheep. Epithelium thickness was significantly correlated with parasite load in large ruminants and the most significant (P < 0.05) decrease in epithelium thickness was in Group B (31.12 ± 1.82 μm) and Group C (31.07 ± 1.68 μm) and a same trend was recorded in small ruminants. Histopathological changes due to Paramphistomum spp. are reported for the first time, which explained the histomorphological and physiological changes in Paramphistomum-infected rumens which might be associated with lowered feed efficiency and productivity in ruminants.
Muhammad Naeem, Muhammad Inamullah Malik, Talha Umar, Swaiba Ashraf, and Adnan Ahmad
Springer Science and Business Media LLC
Kaliyaperumal Rekha, Nalok Dutta, Muthu Thiruvengadam, Mohammad Ali Shariati, Muhammad Usman Khan, Muhammad Usman, Mihir Bhatta, Kunal Ghosh, Shaheer Arif, and Muhammad Naeem
Wiley
Xilin Liu, Zeeshan Nawaz, Caixia Guo, Sultan Ali, Muhammad Ahsan Naeem, Tariq Jamil, Waqas Ahmad, Muhammad Usman Siddiq, Sarfraz Ahmed, Muhammad Asif Idrees,et al.
Frontiers Media SA
Rabies virus (RABV) is a cunning neurotropic pathogen and causes top priority neglected tropical diseases in the developing world. The genome of RABV consists of nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA polymerase L protein (L), respectively. The virus causes neuronal dysfunction instead of neuronal cell death by deregulating the polymerization of the actin and microtubule cytoskeleton and subverts the associated binding and motor proteins for efficient viral progression. These binding proteins mainly maintain neuronal structure, morphology, synaptic integrity, and complex neurophysiological pathways. However, much of the exact mechanism of the viral-cytoskeleton interaction is yet unclear because several binding proteins of the actin-microtubule cytoskeleton are involved in multifaceted pathways to influence the retrograde and anterograde axonal transport of RABV. In this review, all the available scientific results regarding cytoskeleton elements and their possible interactions with RABV have been collected through systematic methodology, and thereby interpreted to explain sneaky features of RABV. The aim is to envisage the pathogenesis of RABV to understand further steps of RABV progression inside the cells. RABV interacts in a number of ways with the cell cytoskeleton to produce degenerative changes in the biochemical and neuropathological trails of neurons and other cell types. Briefly, RABV changes the gene expression of essential cytoskeleton related proteins, depolymerizes actin and microtubules, coordinates the synthesis of inclusion bodies, manipulates microtubules and associated motors proteins, and uses actin for clathrin-mediated entry in different cells. Most importantly, the P is the most intricate protein of RABV that performs complex functions. It artfully operates the dynein motor protein along the tracks of microtubules to assist the replication, transcription, and transport of RABV until its egress from the cell. New remedial insights at subcellular levels are needed to counteract the destabilization of the cytoskeleton under RABV infection to stop its life cycle.
Arslan Iftikhar, Rimsha Nausheen, Humaira Muzaffar, Muhammad Ahsan Naeem, Muhammad Farooq, Mohsin Khurshid, Ahmad Almatroudi, Faris Alrumaihi, Khaled S. Allemailem, and Haseeb Anwar
MDPI AG
Honey is the principal premier product of beekeeping familiar to Homo for centuries. In every geological era and culture, evidence can be traced to the potential usefulness of honey in several ailments. With the advent of recent scientific approaches, honey has been proclaimed as a potent complementary and alternative medicine for the management and treatment of several maladies including various neurological disorders such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and multiple sclerosis, etc. In the literature archive, oxidative stress and the deprivation of antioxidants are believed to be the paramount cause of many of these neuropathies. Since different types of honey are abundant with certain antioxidants, primarily in the form of diverse polyphenols, honey is undoubtedly a strong pharmaceutic candidate against multiple neurological diseases. In this review, we have indexed and comprehended the involved mechanisms of various constituent polyphenols including different phenolic acids, flavonoids, and other phytochemicals that manifest multiple antioxidant effects in various neurological disorders. All these mechanistic interpretations of the nutritious components of honey explain and justify the potential recommendation of sweet nectar in ameliorating the burden of neurological disorders that have significantly increased across the world in the last few decades.
Haider Abbas, Hafiz Muhammad Rizwan, Muhammad Younus, Muhammad Sohail Sajid, Shahid Hussain Farooqi, Zeeshan Iqbal, Muhammad Imran, Usman Amanat, Nida Wazir, and Muhammad Ahsan Naeem
Knowledge E DMCC
Background: Among the blood-borne parasitic infections of dogs, trypanosomosis and babesiosis are highly prevalent in the Indian subcontinent and highly pathogenic.
 Methods: A six-month-old dog was presented with complaint of lethargy, anorexia and loss of body weight over the previous 15 days on October 11, 2021. A full clinical and targeted haematologic and parasitological examination was undertaken.
 Results: Wet smears were positive for the motile trypanosomes while stained thin blood smears showed large number of extracellular trypanosomes and intra-erythrocytic ring-like inclusion bodies of Babesia spp. A high burden of Toxocara canis was identified on coprological examination.
 Conclusion: This report describes the clinical manifestation of concurrent infection of haemoparasites and gastrointestinal nematodes in Pakistani dogs, and highlights the importance of prompt veterinary intervention.
Hafiz Ghulam Muhu-Din Ahmed, Muhammad Naeem, Yawen Zeng, Muhammad Abdul Rehman Rashid, Aziz Ullah, Amjad Saeed, and Abdul Qadeer
Public Library of Science (PLoS)
Dissecting the genetic basis of physiological and yield traits against tolerance to heat stress is an essential in wheat breeding programs to boost up the wheat yield for sustainable food security. Herein, a genome-wide association study (GWAS) was performed to reveal the genetic basis of heat tolerance using high-density Illumina 90K Infinium SNPs array through physiological and yield indices. These indices were phenotyped on a diverse panel of foreign and domestic genotypes of Pakistan, grown in normal and heat-stressed environments. Based on STRUCTURE analysis, the studied germplasm clustered into four sub-population. Highly significant variations with a range of moderate (58.3%) to high (77.8%) heritability was observed under both conditions. Strong positive correlation existed among physiological and yield related attributes. A total of 320 significant (-log10 P ≥ 3) marker-trait associations (MTAs) were identified for the observed characters. Out of them 169 and 151 MTAs were recorded in normal and heat stress environments, respectively. Among the MTA loci, three (RAC875_c103017_302, Tdurum_contig42087_1199, and Tdurum_contig46877_488 on chromosomes 4B, 6B, and 7B respectively), two (BobWhite_c836_422 and BS00010616_51) and three (Kukri_rep_c87210_361, D_GA8KES401BNLTU_253 and Tdurum_contig1015_131) on chromosomes 5A, 1B, and 3D at the positions 243.59cM, 77.82cM and 292.51cM) showed pleiotropic effects in studied traits under normal, heat-stressed and both conditions respectively. The present study not only authenticated the numerous previously reported MTAs for examined attributes but also revealed novel MTAs under heat-stressed conditions. Identified SNPs will be beneficial in determining the novel genes in wheat to develop the heat tolerant and best yielded genotypes to fulfill the wheat requirement for the growing population.
Waqas Ahmad, Muhammad Ahsan Naeem, Qaiser Akram, Sarfraz Ahmad, and Muhammad Younus
Elsevier BV
Madiha Islam, Abdullah, Bibi Zubaida, Nageena Amin, Rashid Iqbal Khan, Noshin Shafqat, Rabia Masood, Shahid Waseem, Jibran Tahir, Ibrar Ahmed,et al.
Frontiers Media SA
Citation: Islam M, Abdullah, Zubaida B, Amin N, Khan RI, Shafqat N, Masood R, Waseem S, Tahir J, Ahmed I, Naeem M and Ahmad H (2021) Agro-Morphological, Yield, and Genotyping-by-Sequencing Data of Selected Wheat (Triticum aestivum) Germplasm From Pakistan. Front. Genet. 12:617772. doi: 10.3389/fgene.2021.617772 Agro-Morphological, Yield, and Genotyping-by-Sequencing Data of Selected Wheat (Triticum aestivum) Germplasm From Pakistan
Abdullah, Furrukh Mehmood, Iram Shahzadi, Zain Ali, Madiha Islam, Muhammad Naeem, Bushra Mirza, Peter J. Lockhart, Ibrar Ahmed, and Mohammad Tahir Waheed
Wiley
The co‐occurrence of mutational events including substitutions and insertions–deletions (InDels) with oligonucleotide repeats has previously been reported for a limited number of prokaryotic, eukaryotic, and organelle genomes. In this study, the correlations among these mutational events in chloroplast genomes of species in the eudicot family Malvaceae were investigated. This study also reported chloroplast genome sequences of Hibiscus mutabilis, Malva parviflora, and Malvastrum coromandelianum. These three genomes and 16 other publicly available chloroplast genomes from 12 genera of Malvaceae were used to calculate the correlation coefficients among the mutational events at family, subfamily, and genus levels. In these comparisons, chloroplast genomes were pairwise aligned to record the substitutions and the InDels in mutually exclusive, 250 nucleotide long bins. Taking one among the two genomes as a reference, the coordinate positions of oligonucleotide repeats in the reference genome were recorded. The extent of correlations among repeats, substitutions, and InDels was calculated and categorized as follows: very weak (0.1–0.19), weak (0.20–0.29), moderate (0.30–0.39), and strong (0.4–0.69). The extent of correlations ranged 0.201–0.6 between “InDels and single‐nucleotide polymorphism (SNP)”, 0.182–0.513 between “InDels and repeat” and 0.055–0.403 between “SNPs and repeats”. At family‐ and subfamily‐level comparisons, 88%–96% of the repeats showed co‐occurrence with SNPs, whereas at the genus level, 23%–86% of the repeats co‐occurred with SNPs in same bins. Our findings support the previous hypothesis suggesting the use of oligonucleotide repeats as a proxy for finding the mutational hotspots.
Muhammad Ahsan Naeem, Waqas Ahmad, Rohit Tyagi, Qaiser Akram, Muhammad Younus, and Xilin Liu
MDPI AG
Tuberculosis is a devastating disease causing high mortality all over the world, especially in the developing countries. Mycobacterium tuberculosis (M. tb) is the causative agent of tuberculosis which replicates in the intracellular environment of host macrophages. Although the host immune system is capable of completely eliminating the pathogen, co-evolution of M. tb with humans has resulted in its ability to hijack the host innate and adaptive immune systems in numerous ways. Limited recent progress has been made in the understanding of M. tb immune escape mechanisms, hence exploration of survival strategies of M. tb has been critically reviewed with an insight into understanding its pathogenesis. We summarized the recent studies regarding the modulation of innate immune response, adaptive immune response, epigenetics and the role of miRNA. All of these advancements suggest that M. tb is well-familiarize with the host immune system and possess the ability to hijack it for intracellular survival.
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)
In my PhD, I have designed sub-unit vaccine against human tuberculosis by applying immunoinformatics approach using its pathogen's secretory proteins. pre-clinical trials were performed in mice model and obtained successful results. Now I along with my PhD supervisor from China are in a plan to patent it.
Industry, Institute, or Organisation Collaboration
I am currently in collaboration with my lab from where I have done my PhD from Huazhong Agricultural University, Wuhan, China in a book preparation.
INDUSTRY EXPERIENCE
I have a basic working knowledge of pharmaceutical industry.