Prof. AMEEN Oloduowo Mubarak (FCSN) had his B. Sc. from Bayero University Kano (BUK, 1997), M. Sc. and PhD from University of Ilorin in 2004 and 2015 respectively. He participated in the National University Commission of Nigeria’s Virtual Institute for Higher Education Pedagogy (NUC - VIHEP) from December, 2003 to May, 2004 where he obtained certificates on the various programmes mounted by the institute during this period. He was awarded a Fulbright JSD scholarship tenable at the University of South Florida, USA, between 2011 and 2012.
He joined the service of the Department of Chemistry, University of Ilorin in 1999 as a Graduate Assistant. He is currently a Reader in the Organic Unit of the Department where he Lectures and supervises both graduate and undergraduate research projects. His research interest is in the Medicinal, Natural Products and Synthetic Chemistry.
He has spread publications in home based, national and international journals.
EDUCATION
Doctor of Philosophy ( in Chemistry, University of Ilorin, 2015; Master of Chemistry (M.Sc.) in Chemistry, University of Ilorin, Ilorin, 2004; B.Sc. (Hons.) Chemistry, Bayero University Kano, 1997
RESEARCH INTERESTS
Isolation, characterization and evaluation of Therapeutic potentials of secondary metabolite from Nigerian Flora; Evaluation of nutritional quality of lesser known oil seeds.
18
Scopus Publications
1613
Scholar Citations
15
Scholar h-index
23
Scholar i10-index
Scopus Publications
Morinda lucida and Annona muricata reduced hepatic lipid peroxidation and promoted melatonin/TNFα/p53-mediated apoptosis in sodium arsenite-induced toxicity in rats Adelaja Akinlolu, Adeoye Oyewopo, Risikat Kadir, Mubarak Ameen, Victor Owoniyi, et al. European Journal of Anatomy, 2022 Arsenic-induced carcinogenesis can result in cancers of the liver in exposed organisms. This study evaluated anticancer potentials of MLF1 and AMF1 extracted from Morinda lucida and Annona muicata leaves respectively in Sodium arsenite (SA)-induced toxicity in rats. Sixty adult female rats were randomly divided into 12 groups (n = 5). Group 1 was control. Group 2 received 5-weeks administrations of 10 mg/kg bodyweight of SA. Groups 3-6 received SA-dose for 2 weeks followed by 3-weeks posttreatments with MLF1-doses and AMF1-doses respectively. Groups 7-10 received only 5-weeks administrations of MLF1-doses and AMF1- doses respectively. Groups 11 and 12 received 5-weeks co-administrations of SA-dose with highdoses of MLF1 and AMF1 respectively. Drugs/ extracts were administered orally. Liver histopathology (Heamatoxylin and Eosin) and ELISA concentrations of sera Melatonin and TNF-alpha were evaluated. Malondialdehyde (thiobarbituricacid assay) and p53 (ELISA) levels were evaluated in liver homogenates. Data were statistically analysed. Results showed normal liver histology in Groups 1-12. Post-treatments of SA-induced toxicity with MLF1 and AMF1 resulted in significant (P≤0.05) and non-significant decreased levels (P≥0.05) of Malondialdehyde, TNF-alpha and p53, but significant (P≤0.05) and non-significant increased Melatonin levels (P≥0.05) in Groups 3-12 compared with Group 2. MLF1 and AMF1 possess anticancer, antioxidant, pro-Melatonin, anti-inflammatory and hepato-protective potentials.
MO11 and MS06 ameliorated cadmium chloride-induced neuro-inflammation, hyperplasia and apoptosis via NF-kB/Caspase-3/p53 pathway and down-regulated sVEGFR in rats Adelaja Akinlolu, Mubarak Ameen, Gabriel Ebito, Nnaemeka Asogwa, Raheem Akindele, et al. European Journal of Anatomy, 2022 Cadmium is a neurotoxin, carcinogen and a suspected agent in aetiology of Parkinson’s disease and Alzheimer’s disease (AD). Furthermore, upregulations of Caspase-3 and p53 were reported in brains of AD patients. This study evaluated the neuroprotective potentials of MO11 (isolated from Moringa oleifera leaves) and MS06 (isolated from Musa sapientum suckers) in Cadmium Chloride (CdCl)-induced neurotoxicity in the cerebrum of rats. Twenty-eight adult male wistar rats (average weight of 155 g) were randomly divided into 7 groups (n = 4). Group 1 received physiological saline. Groups 2-4 and 7 received single 1.5 mg/Kg bodyweight of CdCl (i.p.) (Day 1). Groups 3-4 and 7 were post-treated with 15 mg/Kg bodyweight of MO11, 15 mg/Kg bodyweight of MO11 + 7 mg/Kg bodyweight of MSF1 and 3.35 mg/Kg bodyweight of Doxorubicin respectively (Days 1-17). Groups 5-6 received only MO11 and Vegetable Oil (vehicle) respectively (Days 1-17). Cerebral histopathology (Cresyl Fast Violet method) was evaluated in rats. ELISA evaluations of biomarkers of pro-inflammation (IL-1β, IL-6, IL-8 and NF-kB), anti-inflammation (IL-4 and IL- 10), apoptosis (Caspase-3 and p53), proliferation (Ki67) and angiogenesis (sVEGFR) in cerebral homogenates of rats were also conducted. Histopathological evaluations showed a high number of chromatolytic cells in Group 2, compared with Groups 1 and 3-7. Post-treatments of CdCl-induced neurotoxicity with MO11 and MS06 resulted in decreased levels of IL-1β, IL-6, IL-8, NF-kB, Caspase-3, Ki67, p53 and sVEGFR, but increased levels of IL-4 and IL-10 in Groups 3-4, compared with Group 2. Therefore, MO11 and MS06 possess neuroprotective, neuroregenerative, anti-AD, anti-inflammatory and anticancer potentials. Key words: Cadmium – Neurotoxicity – Alzheimer’s disease – Moringa oleifera – Musa sapientum – Neuroprotection – Neuroregeneration
Extraction, isolation and evaluation of anti-toxic principles from Moringa oleifera (MOF6) and Myristica fragrans (Trimyristin) upregulated Acetylcholinesterase concentrations in Sodium arsenite-induced neurotoxicity in rats. Adelaja Akinlolu, Mubarak Ameen, Tobilola Quadri, Kayode Odubela, Gabriel Omotoso, et al. Journal of Phytomedicine and Therapeutics, 2020 This study evaluated the neuroprotective effects of MOF6 (isolated from Moringa oleifera leaves) and Trimyristin (isolated from Myristica fragrans seeds) on Acetylcholinesterase concentrations in cerebral cortices of rats with Sodium arsenite-induced neurotoxicity. Sixty-five adult male rats (150 g-250 g) were randomly divided into thirteen groups comprising of five rats per group. Groups 1 and 3 received physiological saline and 1 ml/200 g bodyweight of Olive oil respectively for 9 weeks. Group 2 received 20 mg/kg bodyweight of Sodium arsenite (SA) for 6 weeks and left untreated for another 3 weeks. Groups 4-5 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 5.0 and 7.5 mg/kg bodyweight of MOF6 respectively for 6 weeks. Groups 6-7 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 15 and 30 mg/kg bodyweight of Trimyristin respectively for 6 weeks. Groups 8-11 received 5.0 and 7.5 mg/kg bodyweight of MOF6; 15 and 30 mg/kg bodyweight of Trimyristin respectively for 9 weeks. Groups 12-13 received 7.5 mg/kg bodyweight of MOF6 and 30 mg/kg bodyweight of Trimyristin respectively for 6 weeks followed by co-administration of each extract dose with 20 mg/kg bodyweight of SA for another 3 weeks. Histological examination of cerebral cortices and biochemical analyses of Acetylcholinesterase concentrations were carried out in all rats. Computed data were analyzed using Microsoft Excel 2016 with statistical significance at p≤0.05. Histo-pathological evaluations revealed normal histo-architecture of cerebral cortices of all rats. Results showed statistically significant (p≤0.05) increases in Acetylcholinesterase concentrations in rats of Groups 1-10 and 12 compared with Group 2 (2.78±1.76 𝜇mole/min/g). 7.5 mg/kg bodyweight of MOF6 showed the best therapeutic and neuro-regenerative potential against SA-induced neurotoxicity.Conclusions: Our findings implied that MOF6 and Trimyristin reversed downregulation of Acetylcholinesterase concentrations in SA-induced neurotoxicity in rats; and possess neuro-protective and neuro-regenerative potentials.
Characterisation and treatment of spent deposited sludge of transformer oil (DSTO) by acid leaching and solvent extraction Alafara A. Baba, Joshua S. Ayodele, Oloduowo M. Ameen, Abdulrasaq Jimoh, Uduakobong Johnson, et al. Mineral Processing and Extractive Metallurgy Transactions of the Institute of Mining and Metallurgy, 2018 The characterisation of spent transformer oil to determine its fitness for re-refining as well as the recovery of metals by hydrometallurgical treatment in chloride medium has been investigated. The leaching experiments on the spent material showed that its dissolution increases with leachant concentration performed at 75°C. Leaching studies showed that at optimal conditions, the dissolution reached 93% by 1.0 mol L−1 HCl solution within 120 min at 75°C. The solvent extraction of copper by bis(2,2,4-trimethylpentyl) phosphinic acid (CYANEX®272) in kerosene from a pregnant solution of aqueous spent deposited sludge of transformer oil containing 1243.4 mg L−1 Cu++, 389.2 mg L−1 Fe++/+++, 110.7 mg L−1 Ca++ and 235 mg L−1 Al+++ was studied. The results for the solvent extraction give an extraction efficiency of 91.77% total copper acquired via 0.2 mol L−1 CYANEX®272 in kerosene at 27 ± 2°C within 25 min at pH 4.0. A 0.1 mol L−1 HCl solution was found to be sufficient for the stripping of about 94.77% copper from the loaded organic phase. The Fourier transform–infrared spectroscopic analysis of the loaded organic phase supports the stripping experiment as there was no Cu metal ion present in the separated organic phase.
Preservation of smoked African catfish, clarias gariepinus burchell against dermestes maculatus de geer (Coleoptera: Dermestidae) using neem seed oil-iodized salt mixtures Agriculturae Conspectus Scientificus, 2016
Bioactivity of rhizome essential oils from two varieties of Cyperus articulatus (L.) grown in Nigeria, using brine shrimp (Artemia salina) lethality tests Journal of Medicinal Plants Research, 2011
Comparative study of physico chemical parameters of water from a river and its surrounding wells for possible interactive effect Advances in Environmental Biology, 2010
The extraction of proteins from the neem seed (Indica azadirachta A. Juss) African Journal of Biotechnology, 2005
Bioactive metabolites in improved cowpea seeds African Journal of Biotechnology, 2005
RECENT SCHOLAR PUBLICATIONS
Comparative Proximate, Mineral, and Amino Acid Analysis of Processed and Unprocessed Cowhide (Bos taurus) OM Ameen, BZ Arowolo, EC Peteranya, AM Adamu, SR Agboola-Azeez, ... Ilorin Journal of Science 12 (2), 108-115 , 2025 2025
Vernonia Amygdalina restores prednisolone-induced damage to hypothalamic pituitary testicular axis in male wistar rats ER Kadir, AD Yakub, WA El-Hussein, EO Oluwafemi, AT Abdulmaliq, ... Tissue and Cell 96, 103022 , 2025 2025
Neuroprotective Potentials Of Extracts From Moringa Oleifera And Musa Sapientum Against Cadmium Chloride-Induced Neurotoxicity In Rats Cerebri A Akinlolu, M Ameen, T Garuba, N Asogwa, Z Arowolo, B Fagbohunka, ... Journal of Experimental and Molecular Biology 26 (1), 47-58 , 2025 2025
Exploring Climate-Resilient Endophytic Fungi for Sustainable Drug Discovery: Antioxidant Potential of Lasiodiplodia pseudotheobromae in a Changing Climate OM Ameen, OM Adelanwa, AA Abdullateef, G Taofeeq, AB Zainab Centrepoint Journal (Science Edition) 29 (1), 25-36 , 2025 2025
Role of AI in Modernising Accreditation and International Standards in Higher Education MO Ameen Handbook on Artificial Intelligence and Quality Higher Education: AI and … , 2025 2025
Guarding Against Hazard in Laboratories: Navigating Chemical Safety in The Modern Age VI Akinmoladun, AB Akanbi, RC Adeeko, OM Adelanwa, OP Ashaolu, ... International Conference of Faculty of Physical Sciences, University of … , 2024 2024
Preparation and Characterization of Biodegradable Diethanolamide Surfactant from Cotton Seed Oil FR Abdulkadir, OO Oluwaniyi, SO Ibrahim, MO Ameen, KS Jubril International Conference of Faculty of Physical Sciences, University of … , 2024 2024
Chemical Characterization of Colorants Isolated from the Fruit Juice of Anacardium occidentale (Cashew) OM Ameen, SO Abdulrahman Ilorin Journal of Science 11 (3 (Special)), 178-188 , 2024 2024
Phytochemical Analysis and Anti-malarial Activities of the Ethanol Extract of the Leaves of Cassia Fistula OM Ameen, FA Muhammad Ilorin Journal of Science 11 (3 (Special)), 229-236 , 2024 2024
Pharmacognostic Evaluation, Antimicrobial activities and Phytochemical Screening of Selected Medicinal Plants used in Folkloric Medicine for Wound Treatment BZ Arowolo, OM Ameen, E Owen-Obaseki, IB Gegele, OR Opatokun-Saliu Ilorin Journal of Science 11 (3 (Special)), 280-288 , 2024 2024 Citations: 2
Assessment of Phoenix Dactylifera seed extract as a green inhibitor for the corrosion of mild steel in an acidic solution AI OWOLABI, IV Ayinde, YO Busari, RA Yahya, MO Ameen, ... Published by University Politehnica Timsoara, Faculty of Engineering Hunedoara , 2024 2024
Moringa oleifera (MO11) and Musa sapientum (MS06) Promoted Positive Neurotransmitter-Cancer Interaction and Ameliorated Cadmium Chloride-Induced De-Myelination, Hyperplasia … A Akinlolu, M Ameen, G Ebito, N Asogwa, R Akindele, B Fagbohunka CHRISMED Journal of Health and Research 11 (3), 127-133 , 2024 2024
Pharmacognostic, phytochemical, and chemomicroscopic evaluation of Physalis angulata (L) ZB Arowolo, MO Ameen, E Owen-Obaseki, IB Gegele, A Adamu, ... Proceedings of the Nigerian Academy of Science 17 (1), 79-94 , 2024 2024 Citations: 1
Assessment of Phoenix Dactylifera Seed Extract as A Green Inhibitor for The Corrosion of Mild Steel in An Acidic Solution YLS Babata, IV Ayinde, YO Busari, RA Yahya, MO Ameen, ... Annals of Faculty Engineering Hunedoara – International Journal of … , 2024 2024
MORINGA OLEIFERA (MOF6) AND MUSA SAPIENTUM (MSF1) AMELIORATED 7, 12-DIMETHYLBENZ [A] ANTHRACENE-INDUCED SKIN HISTO-PATHOLOGY, INFLAMMATION, HEPATIC OXIDATIVE STRESS AND … B FAGBOHUNKA, A AKINLOLU, M AMEEN, R KADIR, A OYEWOPO, ... New Armenian Medical Journal 18 (1) , 2024 2024
Anti-toxic Extracts from Moringa Oleifera (MO11) and Musa Sapientum (MS06) Ameliorated Cadmium Chloride-induced Neuroalterations of Catalase, Cyclo-oxygenase-2, Superoxide … A Akinlolu, M Ameen, T Garuba, N Asogwa, Z Arowolo, B Fagbohunka, ... Journal of Morphological Sciences Vol 41, 240 , 2024 2024
ANTI-TOXIC PRINCIPLES FROM MUSA SAPIENTUM AND MORINGA OLEIFERA AMELIORATED SKIN HISTO-PATHOLOGY, DECREASED LIPID PEROXIDATION AND PROMOTED MELATONIN/TNF-ALPHA/P53-MEDIATED … A Akinlolu, MO Ameen, AO Oyewopo, RE Kadir, P Nwogu, M Saad, ... Malaysian Journal of Pharmaceutical Sciences 22 (1), 43-59 , 2024 2024
MO11 and MS06 Ameliorated Cadmium Chloride-Induced Neuro-Degeneration and Alterations of Dopamine, Glutamate and Myelin Basic Protein Expressions in Rats A Akinlolu, M Ameen, G Ebito, N Asogwa, R Akindele, B Fagbohunka THE NEW ARMENIAN MEDICAL JOURNAL 18 (1), 46-53 , 2024 2024
Anti-cancer and tissue-neuroprotective potentials of Moringa oleifera and Musa sapientum against in-vivo cadmium chlorideinduced skin toxicity AA Akinlolu, G Ebito, M Ameen, N Asogwa, R Akindele, B Fagbohunka Anatomy Journal of Africa 12 (2), 2402-2413 , 2023 2023 Citations: 1
Cadmium Chloride-induced skin ulceration and inflammation in rats: Anti-inflammatory potentials of Moringa oleifera and Musa sapientium via NF-KB/IL-4/1L-10-mediated pathway. AA Akinlolu, G Ebito, M Ameen, N Asogwa, K Odubela, R Akindele, ... Anatomy Journal of Africa 12 (1), : 2285-2295 , 2023 2023
MOST CITED SCHOLAR PUBLICATIONS
Antioxidants: Its medicinal and pharmacological applications AA Hamid, OO Aiyelaagbe, LA Usman, OM Ameen, A Lawal African Journal of pure and applied chemistry 4 (8), 142-151 , 2010 2010 Citations: 707
Physico-chemical characteristics of oil and biodiesel from Nigerian and Indian Jatropha curcas seeds MA Belewu, FA Adekola, GB Adebayo, OM Ameen, NO Muhammed, ... International Journal of Biological and Chemical Sciences 4 (2), 524-529 , 2010 2010 Citations: 109
Physico-chemical properties of biodiesel produced from Jatropha curcas oil and fossil diesel GB Adebayo, OM Ameen, LT Abass Journal of Microbiology and Biotechnology Research 1 (1), 12-16 , 2011 2011 Citations: 100
COVID-19: Critical discussion on the applications and implications of chemicals in sanitizers and disinfectants O Atolani, MT Baker, OS Adeyemi, IR Olanrewaju, AA Hamid, OM Ameen, ... EXCLI journal 19, 785 , 2020 2020 Citations: 87
Chemical composition of rhizome essential oil of Curcuma longa L. growing in North Central Nigeria LA Usman, AA Hamid, OC George, OM Ameen, NO Muhammad, ... World J. Chem 4 (2), 178-181 , 2009 2009 Citations: 68
Comparison of sorption capacity and surface area of activated carbon prepared from Jatropha curcas fruit pericarp and seed coat OF Okeola, EO Odebunmi, OM Ameen Bulletin of the Chemical Society of Ethiopia 26 (2) , 2012 2012 Citations: 62
Comparative study of physic-chemical parameters of water from a River and its surrounding wells for possible interactive effect FO Okeola, OD Kolawole, OM Ameen Advances in environmental Biology 4 (3), 336-340 , 2010 2010 Citations: 54
The potential of Oleander ( Thevetia peruviana ) in African agricultural and industrial development: a case study of Nigeria. LA Usman, OO Oluwaniyi, SA Ibiyemi, NO Muhammad, OM Ameen 2009 Citations: 54
The extraction of proteins from the neem seed ( Indica azadirachta A. Juss) LA Usman, OM Ameen, SA Ibiyemi, NO Muhammad African Journal of Biotechnology 4 (10) , 2005 2005 Citations: 28
Chemical composition of leaf essential oil of Annona senegalensis Pers. (Annonaceae) growing in North Central Nigeria OM Ameen, LA Usman, FS Oganija, AA Hamid, NO Muhammed, ... International Journal of Biological and Chemical Sciences 5 (1) , 2011 2011 Citations: 19
Moringa oleifera ameliorates cuprizone-induced cerebellar damage in adult female rats GO Omotoso, RE Kadir, SF Lewu, IT Gbadamosi, AA Akinlolu, ... Research journal of health sciences 6 (1), 13-25 , 2018 2018 Citations: 18
Moringa oleifera and Musa sapientum ameliorated 7, 12-Dimethylbenz [a] anthracene-induced upregulations of Ki67 and multidrug resistance 1 genes in rats AA Akinlolu, AO Oyewopo, RE Kadir, A Lawal, J Ademiloye, A Jubril, ... International journal of health sciences 15 (3), 26 , 2021 2021 Citations: 17
Moringa oleifera impairs the morphology and functions of the kidney in adult wistar rats AA Akinlolu, OK Ghazali, OM Ameen, SC Oyebanji, GO Omotoso, ... Int J Morphol 32, 469-474 , 2014 2014 Citations: 17
Bioactivity of rhizome essential oils from two varieties of Cyperus articulatus (L.) grown in Nigeria, using brine shrimp (Artemia salina) lethality tests OM Ameen, LA Usman, IA Oladosu, NO Olawore, IA Ogunwande Journal of Medicinal Plants Research 5 (6), 1031-1033 , 2011 2011 Citations: 16
The effects of Moringa Oleifera on lipid profile status, heart histology, and liver histochemistry in adult Wistar rats AA Akinlolu, EO Bayode, KO Ghazali, MO Ameen CHRISMED Journal of Health and Research 4 (2), 104-109 , 2017 2017 Citations: 15
Solvent-free synthesis, x-ray studies and in vitro inhibitory activities of copper (II) complexes of non-steroidal anti-inflammatory drugs AC Tella, JA Obaleye, UB Eke, AY Isaac, OM Ameen Research on Chemical Intermediates 39 (2), 1-17 , 2013 2013 Citations: 15
Extraction, isolation and evaluation of anti-toxic principles from Moringa oleifera (MOF6) and Myristica fragrans (Trimyristin) upregulated Acetylcholinesterase concentrations … A Akinlolu, M Ameen, T Quadri, K Odubela, G Omotoso, R Yahya, ... Journal of Phytomedicine and Therapeutics 19 (2), 466-482 , 2020 2020 Citations: 14
Preservation of smoked African catfish, Clarias gariepinus Burchell against Dermestes maculatus De Geer (Coleoptera: Dermestidae) using neem seed oil-iodized salt mixtures SA Babarinde, TA Adebayo, LA Usman, OM Ameen, AO Akinyemi, ... Agriculturae Conspectus Scientificus 81 (4), 235-240 , 2016 2016 Citations: 13
Effect of alkaline hydrolysis on the quantity of extractable protein fractions (prolamin, albumin, globulin and glutelin) in Jatropha curcas seed cake LA Usman, OM Ameen, A Lawal, GV Awolola African Journal of Biotechnology 8 (22) , 2009 2009 Citations: 12
Bioactive metabolites in improved cowpea seeds OM Ameen, OM Fatope, LA Usman, SA Adebayo African Journal of Biotechnology 4 (6), 513-516 , 2005 2005 Citations: 12
