Usmangani K Chhalotiya

Scopus Publications

Planer chromatographic method development and validation of Tadalafil and Mirabegron using fractional factorial design approach
Ghanshyam B Paladiya, Jinal Tandel, Heta Kachhiya, Usmangani Chhalotiya, Dimal Shah, Devang Tandel
Journal of Chromatographic Science, 2026
The present research focuses on developing a high-performance thin-layer chromatographic (HPTLC) method using a design of experiment approach for simultaneously estimating Tadalafil and Mirabegron. Chromatographic separation was achieved on aluminum plates precoated with silica gel 60 F254 using an optimized mobile phase of MeOH: ACN: n-Butanol: TEA (6:2:2:0.1%v/v/v/v). A fractional factorial design was applied for the robustness study and the independent variables selected were chamber saturation time (A), wavelength (B), mobile phase composition (C) and solvent front (D). It was statistically revealed that the volume of MeOH affected the Rf of both drugs resulting in stricter control of MeOH volume compared to the other three variables. Rf for Tadalafil and Mirabegron was 0.75 and 0.45 at 242 nm. Calibration plots were linear for Tadalafil (100–300 ng/band) and Mirabegron (500–1500 ng/band), with recovery rates of 98.15%–100.34% and 98.46%–102.14%, respectively. The method was validated per ICH Q2(R1) guidelines, demonstrating linearity, precision, accuracy and selectivity, with %RSD values below 2% for both drugs. This HPTLC method is simple, accurate, reproducible and suitable for routine quality control of pharmaceutical formulations.
Development of stability indicating densitometric method for estimation of tegoprazan used in the treatment of gastroesophageal reflux disease
Mitalben Parmar, Dimal Shah, Usmangani Chhalotiya
Journal of Research in Pharmacy, 2026
Novel drug Tegoprazan has been introduced for the treatment of gastro-esophageal reflux disease. Accurate and precise thin layer chromatographic method has been developed and validated for estimation of tegoprazan. Silica gel G 60 F254 (20cm×20cm) coated aluminium backed plates were used as stationary phase and mixture of Toluene: Methanol (7.5: 2.5 v/v) was used as mobile phase. The compact band (Rf values 0.53 ± 0.1) was obtained for Tegoprazan. The method was found to be linear in the concentration range of 100 -700 ng/band with a linear regression equation showing linear relationship with R2 =0.9944. The method was validated as per ICH Q2 (R2) guidelines Forced degradation studies were carried out to assess molecule’s intrinsic stability, using acid and base hydrolysis, oxidative stress and photolytic degradation. Forced degradation study revealed that Tegoprazan was highly susceptible to acid hydrolysis. Greenness assessment for develop method was conducted using AGREE and White analytical chemistry. The evaluation confirmed environmentally friendly nature of developed method.
Computational discovery of uterotonic agents: Molecular docking and dynamic simulations of Caesalpinia bonduc phytoconstituents
Jinal Tandel, Usmangani Chhalotiya, Heta Kachhiya, Ashish Patel, Parth Thakor, Jignesh Prajapati, Dweipayan Goswami
Journal of Research in Pharmacy, 2025
Molecular modeling is widely applied to study the interaction and binding affinity of biological activity ofprotein and peptide. Molecular dynamics simulation can provide valuable information in deciphering functionalmechanisms of protein and other biomolecules, Caesalpinia bonduc (L) Roxb contains compounds that bind with estrogenreceptors, PGs receptors, Oxytocin receptors, Alpha-adrenergic receptors, etc. which are found on the surface ofmyometrial cells that affect contractility. The present study focuses on understanding the molecular mechanism ofbioactive compounds of Caesalpinia bonduc (L) Roxb for uterine contraction. The molecular docking of compounds thatare present in Caesalpinia bonduc (L) Roxb named α caesalpin, β caesalpin, ε caesalpin, caesalpin F, and Bonducellin wereperformed against 1FDS, 3S79, 1E3G, 3ERT targets. Molecular docking and dynamic simulation studies were establishedby applying Glide precision mode and Maestro respectively. The Molecular dynamic (MD) simulation was performed toanalyse the stability and interactions of the bioactive compounds from Caesalpinia bonduc (L) Roxb leaf extract. Dockinganalysis for active biomarkers of Caesalpinia bonduc (L) Roxb with target proteins revealed compound binding towardsselected proteins and activity against uterus contraction. Bonducellin exhibits the highest binding scores among allcompounds, the remaining α caesalpin, ε caesalpin, β caesalpin, and caesalpin F, were also found to have prominentbinding towards selected proteins. This stabilization is achieved through strong and stable interactions, as evidenced bythe MD simulation data. Among the compounds. The results suggest that Bonducellin and related compounds holdpromise as multi-target Uterotonic agents, with potential applications in managing labour and reducing postpartumhaemorrhage..
Validated Green Densitometric Method for Estimation of Naringin Biomarker in Hydroalcoholic Extract of Leaves of Ceiba Pentandra (L.) Gaertn
Asian Journal of Green Chemistry, 2025
Accelerated Stability Indicating LC Method for Simultaneous Quantification of Dapagliflozin Propanediol Monohydrate and Bisoprolol Fumarate
Gopal Valiya, Hetaben Kachhiya, Ruturaj Sutarsandhiya, Riddhi Patel, Jinal Tandel, Usmangani Chhalotiya, Dimal Shah
Separation Science Plus, 2025
A combination of dapagliflozin propanediol monohydrate and bisoprolol fumarate was approved by a regulatory agency for the treatment of Type 2 diabetes mellitus in patients with hypertension. A simple reverse‐phase LC method was developed and validated for simultaneous quantification of dapagliflozin propanediol monohydrate and bisoprolol fumarate in bulk and marketed formulation. The optimized chromatographic condition includes Inertsil ODS 3 V (250 mm × 4.6 mm, 5 µm) as the stationary phase, gradient elution of mobile phase A containing 26 mM KH2PO4 containing 0.4 mL/L triethylamine (pH 4.2 adjusted with dilute orthophosphoric acid) and acetonitrile in ratio of 90:10 v/v and mobile phase B containing 26 mM KH2PO4 containing 0.4 mL/L triethylamine (pH 4.2 adjusted with dilute orthophosphoric acid) and acetonitrile in ratio of 25:75 v/v with flow rate of 1 mL/min. The analytical wavelength for detection of both drugs was 223 nm. The forced degradation study was also carried out to estimate the stability of drugs in bulk and formulation. The stress condition applied were acid, alkali, oxidative, thermal, and light. Dapagliflozin propanediol monohydrate was found to be stable and bisoprolol fumarate was degrading under oxidative and light conditions and remaining stable in other conditions applied. The proposed method was found to be linear in the concentration range of 2–24 µg/mL and 1–12 µg/mL with R2 value of 0.9998 and 0.9984 for dapagliflozin propanediol monohydrate and bisoprolol fumarate, respectively. The optimized method was validated as per ICH Q2 (R2) guidelines and was found to be selective, specific, precise, accurate, and robust for quantification of both drugs.
Stability-indicating instrumental thin-layer chromatography method for the quantification of dapagliflozin propanediol monohydrate and bisoprolol fumarate with implementation of fractional factorial design
Gopal Valiya, Hetaben Kachhiya, Riddhi Patel, Jinal Tandel, Usmangani Chhalotiya, Dimal Shah, Mehul Patel
Journal of Planar Chromatography Modern TLC, 2025
Advanced thin-layer chromatography‒mass spectrometry validation and comprehensive analysis of bioactive phytochemicals in Caesalpinia bonduc leaf extract
Jinal N. Tandel, Usmangani Chhalotiya, Heta Kachhiya, Devang Tandel
Journal of Planar Chromatography Modern TLC, 2025
In silico ADMET Study, Molecular Docking and Dynamic Simulation of Tuber Extract of Amorphophallus paeoniifolius for Anti-inflammatory Target Prediction in Asthma
Heta Patel, Usmangani Chhalotiya, Jinal Tandel, Ashish Patel, Ranjitsinh Dabhi
Chemistry Africa, 2025
Stability indicating HPTLC–densitometric method for estimation of vonoprazan fumarate
Mitalben S. Parmar, Dimal A. Shah, Usmangani K. Chhalotiya
Drug Development and Industrial Pharmacy, 2025
OBJECTIVES High performance thin layer chromatography method was developed and validated for analysis of vonoprazan fumarate. An Alkaline forced degradation kinetic study was performed to find out probable rate of degradation of vonoprazan fumarate. MATERIAL AND METHODS Aluminum packed TLC plates precoated with silica gel 60F 254 were used as stationary phase and Methanol: Toluene: triethylamine (6: 4: 0.1 v/v/v) was used as mobile phase. The detection was carried out at 267 nm wavelength as absorbance mode. HPTLC/MS analysis study was performed to detect alkaline degradant. RESULTS A compact band (Rf value of 0.43 ± 0.1) was obtained for vonoprazan fumarate. Regression analysis shows a good linear relationship (R2 = 0.9996) between peak area and concentration in the range 200-1200 ng/band. The accuracy determined by standard addition method for vonoprazan fumarate and percentage recovery was found to be 99.72% - 101.74%. Forced degradation stability study was performed under different stress conditions including hydrolytic, oxidative, thermal and photolytic. Degradation was observed under alkaline condition and it was found to follow first order degradation kinetic. The possible structure of base degradants was also determined using HPTLC-MS analysis. The method was successfully applied for the estimation of drug in synthetic mixture. CONCLUSION A New, Simple, precise and accurate method has been developed and validated for the quantification of vonoprazan fumarate. Forced degradation studies were performed. The method can be used for quality control and stability sample evaluation of vonoprazan fumarate.
Simultaneous estimation of biomarkers in hydroalcoholic tuber extract of Amorphophallus paeoniifolius by a validated instrumental thin-layer chromatography method
Heta Patel, Usmangani Chhalotiya, Jinal Tandel
Journal of Planar Chromatography Modern TLC, 2024
Simultaneous estimation of dapagliflozin and linagliptin using reverse phase-HPLC with photo diode array (PDA)
Usmangani K. Chhalotiya, Anchal Shukla, Dimal Shah, Jinal Tandel, Hetaben Kachhiya, Mital Parmar
Journal of Chemical Metrology, 2024
HPTLC–densitometric method of analysis for estimation of efonidipine hydrochloride and telmisartan used in treatment of hypertension
Krunal H. Solanki, Dhaval P. Solanki, Dimal A. Shah, Usmangani K. Chhalotiya, Heta M. Kachhiya, Jinal N. Tandel, Mital S. Parmar
Journal of Liquid Chromatography and Related Technologies, 2024
A sensitive, precise, accurate, and economic planar chromatographic method developed and validated for quantification of Desidustat as per guideline
Rutvik Pandya, Heta M. Kachhiya, Dhaval Solanki, Jinal Tandel, Usmangani Chhalotiya, Dimal Shah
Separation Science Plus, 2023
Reversed phase-HPLC-PDA method for quantification of Desidustat
Hetaben Kachhiya, Rutvik Pandya, Krunal Solanki, Jinal Tandel, Usmangani Chhalotiya, Dimal Shah
Journal of Chemical Metrology, 2023
Development of thin layer chromatographic densitometric method for simultaneous quantification of Ivabradine HCl and Bisoprolol fumarate
Mansi Patel, Usmangani K Chhalotiya, Dimal A Shah, Jinal N Tandel, Hetaben M Kachhiya
Separation Science Plus, 2023
Liquid chromatographic method for simultaneous estimation of anti-hypertensive drug combination Azilsartan Medoxomil and Cilnidipine
Dhavalsinh P. Solanki, Vishal R. Panday, Dimal A. Shah, Usmangani Chhalotiya, Jinal N. Tandel, Heta M. Kacchiya
Separation Science Plus, 2023
HPTLC-Densitometric Estimation of Anti-hypertensive Drug Combination Azilsartan Medoxomil and Cilnidipine in Combined Dosage Form
Dhavalsinh P. Solanki, Krunal H. Solanki, Jaineel V. Desai, Dimal A. Shah, Usmangani K. Chhalotiya
Analytical Chemistry Letters, 2023
Reversed-phase–high-performance liquid chromatography method simultaneous quantification of Ivabradine hydrochloride and Bisoprolol fumarate
Mansi M. Patel, Usmangani K. Chhalotiya, Dimal A. Shah, Hetaben M Kachhiya, Jinal N. Tandel
Separation Science Plus, 2022
Stability indicating planar chromatographic method for estimation of minoxidil and finasteride combination used in the treatment of hair loss
J. Patel, Jinal N. Tandel, U. Chhalotiya, Kirtan Patel
Journal of Medicinal and Chemical Sciences, 2021
Simultaneous quantification of brexpiprazole and sertraline HCl in synthetic mixture by thin-layer chromatography method
Shifa Vahora, Usmangani K. Chhalotiya, Hetaben Kachhiya, Jinal Tandel, Dimal Shah
Journal of Planar Chromatography Modern TLC, 2021
Thin-layer chromatographic‒densitometric method of analysis for the estimation of montelukast and bilastine in combination
Dimal A. Shah, Pankti A. Patel, Usmangani Chhalotiya
Journal of Planar Chromatography Modern TLC, 2021
Thin layer chromatographic method for separation and estimation of anticancer drug Ibrutinib in presence of its degradants
Dimal A. Shah, Jesheeka M. Patel, Ashok Mahajan, Usmangani Chhalotiya
Separation Science Plus, 2021
Simultaneous Quantification of Betulinic Acid, Lupeol, and β-Sitosterol in Madhuca longifolia Methanolic Extract of Bark by Liquid Chromatography-Tandem Mass Spectrometric Method
Veena S Patel, Usmangani K Chhalotiya, Sandip B Patel, Jivani Nuruddin
Journal of AOAC International, 2021
SIMULTANEOUS QUANTIFICATION of MINOXIDIL and FINASTERIDE in BULK and PHARMACEUTICAL DOSAGE FORM by RP-HPLC METHOD
P. Jay, Jinal N. Tandel, Usmagani Chhalotiya, Kirtan Patel
European Chemical Bulletin, 2021
Quantification of β-Adrenergic Receptor Agonist Drug Mirabegron in Presence of Degradants by High Performance Thin Layer Chromatography
Preetiben N. Yadav, Usmangani K. Chhalotiya, Heta M. Kachhiya, Keshaben M. Patel, Dimal A. Shah
Analytical Chemistry Letters, 2021
Simultaneous estimation of amlodipine besylate, Rosuvastatin calcium and Fimasartan potassium trihydrate combination used in the treatment of hypertension using LC method
Devansh A. Kansara, Usmangani K. Chhalotiya, Heta M. Kachhiya, Ishita M. Patel, Dimal A. Shah
SN Applied Sciences, 2020
Simultaneous quantification of perindopril erbumine, indapamide and amlodipine besylate in newer combination of anti-hypertensive drugs in pharmaceutical dosage form by thin layer chromatography method
Kirtan Patel, Usmangani Chhalotiya, Hetaben Kachhiya, Jay Patel, Dimal Shah, Dhruti Nagda
Separation Science Plus, 2020
Densitometric simultaneous estimation of combination of empagliflozin, linagliptin and metformin hydrochloride used in the treatment of type 2 diabetes mellitus
Ishita M. Patel, Usmangani K. Chhalotiya, Harsha D. Jani, Devansh Kansara, Dimal A Shah
Journal of Planar Chromatography Modern TLC, 2020
Development of tlc method for simultaneous estimation of novel combination of amlodipine besylate, rosuvastatin calcium, and fimasartan potassium in synthetic mixture
Devansh Kansara, Usmangani K. Chhalotiya, Hetaben M. Kachhiya, Ishita Patel
Journal of Chemical Metrology, 2020
Stability indicating liquid chromatographic method for the estimation of remogliflozin etabonate
Dimal Shah, Ishita Gondalia, Vandana Patel, Ashok Mahajan, Usmangani K. Chhalotiya
Journal of Chemical Metrology, 2020
Stability Indicating TLC Method for Quantification of Brexpiprazole in Bulk and Its Pharmaceutical Dosage Form and Determination of Content Uniformity
Anjali M Thakkar, Usmangani K Chhalotiya, Nikunj Parekh, Jaineel V Desai, Dimal A Shah
Journal of Chromatographic Science, 2019
High-performance thin-layer chromatographic method for the estimation of mirabegron and solifenacin succinate used in the treatment of overactive bladder syndrome
Dimal A. Shah, Pooja J. Tahilramani, Vandana B. Patel, Usmangani Chhalotiya
Journal of Planar Chromatography Modern TLC, 2019
Development of stability indicating HPTLC method for estimation of antihypertensive drug combination nifedipine and valsartan
Dimal A. Shah, Jaimin S. Patel, Priyanka Jadeja, Vandana B. Patel, Usmangani K. Chhalotiya
Journal of Taibah University for Science, 2019
Quantification of newly discovered anti-cancer drug enzalutamide in bulk and synthetic mixture by stability indicating TLC method
Dharmendra Jayantibhai Prajapati, Usmangani Khalilurraheman Chhalotiya, Minesh Dahyabhai Prajapati, Jalpa Upendrabhai Patel, Jaineel Vinodrai Desai
Current Drug Discovery Technologies, 2019
Stability-indicating high-performance thin-layer chromatographic method for the estimation of antipsychotic drug combination clozapine and aripiprazole
Vandana B. Patel, Dimal A. Shah, Hemaxi B. Gohil, Usmangani Chhalotiya
Journal of Planar Chromatography Modern TLC, 2018
Estimation of newer anti-cancer drug clofarabine in their pharmaceutical dosage form by stability indicating TLC method
Brijesh Patel, Usmangani K. Chhalotiya, Sandip B. Patel
Journal of the Chilean Chemical Society, 2018
A synchronous fluorescence spectrofluorometric method for the simultaneous determination of clonazepam and paroxetine hydrochloride in combined pharmaceutical dose form
Jalpa U. PATEL, Usmangani K. CHHALOTIYA, Purvi A. SHAH
Turkish Journal of Pharmaceutical Sciences, 2017
Estimation of ibuprofen and famotidine in tablets by second order derivative spectrophotometery method
Dimal A. Shah, Dixita J. Suthar, Chirag D. Nagda, Usman K. Chhalotiya, Kashyap K. Bhatt
Arabian Journal of Chemistry, 2017
Development of HPTLC method for the estimation of Pioglitazone and Alogliptin in synthetic mixture
Indian Drugs, 2017
Thin-layer chromatography method for the simultaneous quantification and stability testing of alprazolam and mebeverine in their combined pharmaceutical dosage form
Usmangani K. Chhalotiya, Nishma M. Patel, Dimal A. Shah, Falgun A. Mehta, Kashyap K. Bhatt
Journal of Taibah University for Science, 2017
Stability indicating HPTLC method for the estimation of ticagrelor in bulk and in pharmaceutical dosage form
Indian Drugs, 2016
Simultaneous estimation of moxonidine and amlodipine besylate in their pharmaceutical dosage form by stability-indicating LC method
Indian Drugs, 2016
Development of HPTLC method for the estimation of ondansetron and ranitidine in combined dosage form
Indian Drugs, 2015
Simultaneous Quantification of Metolazone and Ramiprilin Their Combined Dosage Form by First Order Derivative Spectroscopic Method
Turkish Journal of Pharmaceutical Sciences, 2015
Development and validation of liquid chromatography-tandem mass spectrometric method for the quantification of ciprofibrate from human plasma
D. Shah, D. Mavani, H. Patel, S. Baldania, K. Bhatt, U. Chhalotiya
Journal of Chromatographic Science, 2015
High-performance thin-layer chromatography method for estimating the stability of a combination of irbesartan and amlodipine besylate
Dimal A. Shah, Dipal V. Patel, Falgun A. Mehta, Usmangani K. Chhalotiya, Kashyap K. Bhatt
Journal of Taibah University for Science, 2015
Development and validation of a liquid chromatographic method for estimation of dicyclomine hydrochloride, mefenamic acid and paracetamol in tablets
Indian Journal of Pharmaceutical Sciences, 2014
Simultaneous estimation of amiloride hydrochloride and torsemide in their combined dosage forms by high-performance thin-layer chromatography
Smit Bhadja, Usmangani Chhalotiya, Dimal Shah, Falgun Mehta, Kashyap Bhatt
Journal of Planar Chromatography Modern TLC, 2014
Development and validation of hptlc method for estimation of ibuprofen and famotidine in pharmaceutical dosage form
Dimal A. Shah, Dixita J. Suthar, Chirag D. Nagda, Usman K. Chhalotiya, Kashyap K. Bhatt
Journal of Liquid Chromatography and Related Technologies, 2014
High-performance thin-layer chromatographic method for the estimation of paracetamol, dicyclomine hydrochloride, and mefenamic acid in combined tablet dosage form
Dimal Shah, Jainika Rana, Sunil Baldania, Usmangani Chhalotiya, Kashyap Bhatt
Journal of Planar Chromatography Modern TLC, 2014
Quantification of clioquinol in bulk and pharmaceutical dosage forms by stability indicating LC method
Turkish Journal of Pharmaceutical Sciences, 2014
Development of LC method for estimation of diethyl carbamazine citrate and chlorpheniramine maleate in combined dosage form
Turkish Journal of Pharmaceutical Sciences, 2014
Comparative pharmacokinetic studies of fast dissolving film and oral solution of ondansetron in rats
Dhagla Choudhary, Vishnu Patel, Usmangani Chhalotiya, Harsha Patel, Aliasgar Kundawala
Current Drug Delivery, 2013
Development of hptlc method for the estimation of antidepressant drugs melitracen and flupentixol in their combined dosage form
Usmangani K. Chhalotiya, Kashyap K. Bhatt, Dimal A. Shah, Chirag D. Nagda, Jigar R. Patel
Journal of Liquid Chromatography and Related Technologies, 2013
Development and characterization of pharmacokinetic parameters of fast-dissolving films containing levocetirizine
Dhagla R. Choudhary
Scientia Pharmaceutica, 2012
Stability-indicating liquid chromatographic method for the quantification of the new antipsychotic agent Asenapine in bulk and in pharmaceutical formulation
Usmangani K. Chhalotiya
Scientia Pharmaceutica, 2012
Natural polysaccharides as film former: A feasibility study for development of rapid dissolving films of ondansetron hydrochloride
International Journal of Pharmacy and Pharmaceutical Sciences, 2012
Stability-indicating liquid chromatographic method for quantification of new anti-epileptic drug lacosamide in bulk and pharmaceutical formulation
Usmangani Chhalotiya, Kashyap Bhatt, Dimal Shah, Sunil Baldania, Jigar Patel
Chemical Industry and Chemical Engineering Quarterly, 2012
Development and validation of HPTLC method for the estimation of desvenlafaxine in tablet formulation
Dimal Shah, Riddhi Nakrani, Sunil Baldania, Usmangani Chhalotiya, Kashyap Bhatt
Journal of Planar Chromatography Modern TLC, 2012
Stability indicating LC-Method for estimation of paracetamol and lornoxicam in combined dosage form
Dimal A. SHAH
Scientia Pharmaceutica, 2011
Liquid chromatographic method for the estimation of donepezil hydrochloride in a pharmaceutical formulation
International Journal of Chemtech Research, 2011
Stability indicating liquid chromatographic method for the estimation of desvenlafaxine in pharmaceutical dosage form
Dimal Shah, Riddhi Nakrani, Sunil Baldania, Usmangani Chhalotiya, Kashyap Bhatt
Chemical Industry and Chemical Engineering Quarterly, 2011
Development and validation of a stability-indicating RP-HPLC method for duloxetine hydrochloride in its bulk and tablet dosage form
Usmangani K. Chhalotiya
Scientia Pharmaceutica, 2010
Development and validation of an ultra performance liquid chromatography method for venlafaxine hydrochloride in bulk and capsule dosage form
UK Chhalotiya, KK Bhatt, HB Patel
Indian Journal of Pharmaceutical Sciences, 2010

Usmangani K Chhalotiya

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Scopus Publications