Mario Sousa Diniz

Scopus Publications

Toxicopathology and expression of a CYP1-like gene in gills and digestive gland of wild Mediterranean mussel (Mytilus galloprovincialis) from sites with different contamination profiles and exposed to B[a]P
J.D. Vitorino, A.P. Rodrigo, M. Loureiro, M.S. Diniz, P.M. Costa
Marine Pollution Bulletin, 2026
Evidence of Endocrine Disruption and Oxidative Stress in Mytilus galloprovincialis Exposed to 17α-Ethinylestradiol
Sandra Copeto, Inês João Ferreira, Catarina Mansilha, Armindo Melo, Carla Motta, et al.
Journal of Marine Science and Engineering, 2026
The presence of endocrine-disrupting compounds (EDCs) in aquatic environments has raised significant concerns, particularly regarding their impact on marine biota. Among these compounds, 17α-ethinylestradiol (EE2), a synthetic estrogen widely used in oral contraceptives, is highly persistent and biologically active at very low concentrations. This study evaluated the effects of EE2 exposure on oxidative stress responses and endocrine disruption in Mytilus galloprovincialis, exposed for 28 days to three EE2 concentrations (10, 30, and 300 ng·L−1). Biomarkers of oxidative stress, including the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione-S-transferase (GST), as well as Lipid Peroxidation (MDA levels), total ubiquitin (UBI) and the endocrine disruption marker, vitellogenin-like protein (VTG) were assessed. Results showed significant increase in GST and a decrease in CAT activities followed by an elevation at 300 ng·L−1, slightly higher than control values. Overall, results suggest an enhanced oxidative challenge. No significant changes were detected in MDA and UBI levels. VTG-like protein levels increased according to the EE2 concentrations tested, suggesting an effect on the mussel’s endocrine system. These results show the activation of detoxification and antioxidant defense mechanisms in exposed mussels as a response to mitigate oxidative stress damage. Furthermore, it highlights the importance of using biomarkers in pollution monitoring studies and environmental risk assessment.
Do not despise the little ones: population-level responses of common gobies to seasonality along latitudinal cline show biased vulnerability of southern populations
Carolina Madeira, Madalena Missionário, Diana Madeira, Carla António, Ana M. Rodrigues, et al.
Marine Environmental Research, 2026
Low oxidative stress of cephalopod early life stages under chronic and intermittent hypoxia
Mélanie Court, Marta Macau, Tânia Marquês, Tiago Repolho, Mário Diniz, et al.
Marine Biology, 2026
Laminaria ochroleuca as a natural remediator of copper-based antifouling agents in aquaculture systems
Rui Cereja, Alícia Pereira, Ana C. Brito, Carla Pires, Mário Diniz, et al.
Marine Pollution Bulletin, 2026
What Type of Compounds are Expected in Halophyte Plants? Do They Have Potential Benefits for Human Consumption?
Inês João Ferreira, Ana Rita C. Duarte, Mário Diniz, Ricardo Salgado
Lecture Notes on Multidisciplinary Industrial Engineering, 2026
Screening pollution biomarkers and burrowing population profiles in an infaunal key species from port estuarine areas: What does deeper sediment say?
M. Díaz-Jaramillo, M.L. Ballesteros, G. Chiang, C.N. Pegoraro, M. Diniz
Marine Pollution Bulletin, 2026
Interactive Effects of Laminaria digitata Supplementation and Heatwave Events on Farmed Gilthead Seabream Antioxidant Status, Digestive Activity, and Lipid Metabolism
Rita V. C. Gomes, Isa Marmelo, Tomás Chainho, Alícia Pereira, Daniel Bolotas, et al.
Environments Mdpi, 2026
Extreme weather events, particularly marine heatwaves (MHWs), increasingly threaten aquaculture systems worldwide by impairing animal physiology and economical sustainability. This showcases the need to develop nutritional approaches that enhance animal performance under sub-optimal conditions. This study evaluated the effects of dietary supplementation with the brown macroalga Laminaria digitata (whole dried powder or extract) on the antioxidant status, digestive activity, and lipid metabolism of juvenile Sparus aurata exposed to a simulated MHW. Fish were fed four diets (control, 0.3% extract, and 0.3% or 1.5% powder) for 30 days before being exposed to a category III Mediterranean MHW. Under optimal temperature, macroalgae supplementation reduced oxidative status (lower catalase activity). The powder-feeds decreased lipid peroxidation, while the extract-feed elicited the opposite. All supplemented diets reduced proteolytic activity, and the extract-feed also decreased amylase activity. The MHW impaired gastrointestinal antioxidant defenses and liver lipid metabolism, decreasing catalase and glutathione S-transferase activities, as well as ΣPUFA n-6, 16:1 n-7, and 18:2 n-6 levels. The 0.3% powder-feed mitigated MHW-induced reductions in antioxidant activity, while both 0.3%-diets prevented thermal stress-related alterations on fatty acid profile. Overall, L. digitata powder at 0.3% was most effective at enhancing thermal stress resilience, supporting its value as a functional aquafeed ingredient.
Napyradiomycin-based coatings: Promising eco-friendly, sustainable and circular solutions for effective marine antibiofouling
Isabel Pereira, Helena Macedo, Inês Ferreira, Bárbara Gonçalves, Susana Piçarra, et al.
Environmental Technology and Innovation, 2025
Development of Innovative Mediterranean-Style Semi-Hard Goat’s Cheese Supplemented with Seaweeds (Palmaria palmata and Ulva sp.) and Its Characterization
Bruno M. Campos, Bruno S. Moreira-Leite, Abigail Salgado, Edgar Ramalho, Isa Marmelo, et al.
Applied Sciences Switzerland, 2025
The main objective of this study was the development of two semi-hard goat cheeses supplemented with Palmaria palmata and Ulva sp. with the aim of developing innovative food products, increasing the concentration of nutrients in these cheeses and familiarizing consumers with seaweed-containing foods. The impact of seaweed addition was evaluated through physicochemical, microbiological, and organoleptic properties of the semi-hard goat cheeses. Carbohydrate content was relatively low, whereas the total lipid content was relatively high (particularly in semi-hard goat cheese supplemented with seaweeds). Crude protein content presented higher values in semi-hard goat cheese supplemented with Ulva sp. The semi-hard goat cheese supplemented with Ulva sp. shows increased levels of Ca, Fe, Mn, and Zn. Instrumental color and the textural parameters of semi-hard goat’s cheese varied significantly with seaweed addition. Most of the microbiological load complies with the Portuguese (INSA) and the United Kingdom’s (HPA) guidelines for assessing the microbiological safety of ready-to-eat foods placed on the market. Additionally, the Flash Profile scores of semi-hard goat cheeses supplemented with seaweeds highlighted aroma and flavor complexity. Overall, this study confirms the potential of using seaweeds as a viable alternative to produce semi-hard goat cheeses with less pungency or goat milk flavor, making this product more pleasant and appealing to consumers sensitive to these sensory characteristics.
Asparagopsis taxiformis Feed Supplementation as a Tool to Improve the Resilience of Farmed Diplodus sargus to Marine Heatwave Events—A Metabolomics Approach
Marta Dias, Isa Marmelo, Carla António, Ana M. Rodrigues, António Marques, et al.
Fishes, 2025
Improving Farmed Juvenile Gilthead Seabream (Sparus aurata) Stress Response to Marine Heatwaves and Vibriosis Through Seaweed-Based Dietary Modulation
Alícia Pereira, Isa Marmelo, Tomás Chainho, Daniel Bolotas, Marta Dias, et al.
Animals, 2025
Laminaria digitata Supplementation as a Climate-Smart Strategy to Counteract the Interactive Effects of Marine Heatwaves and Disease Outbreaks in Farmed Gilthead Seabream (Sparus aurata)
Isa Marmelo, Tomás Chainho, Daniel Bolotas, Alícia Pereira, Busenur Özkan, et al.
Environments Mdpi, 2025
Molecular landscape of therapeutic deep eutectic systems selective toxicity towards colorectal cancer
Filipe Oliveira, Joana Pinto, Filipa Amaro, Joana Pereira, Inês Ferreira, et al.
Sustainable Chemistry and Pharmacy, 2025
Incorporating Ascorbic Acid into Natural Deep Eutectic Systems: A Study on Antioxidant Preservation
Liane Meneses, Inês João Ferreira, Ana Rita C. Duarte, Mário Diniz, Alexandre Paiva
ACS Food Science and Technology, 2025
Isolation, Fixation and Characterization of Juvenile Gilthead Seabream Head Kidney Leukocytes by Flow Cytometry
Isa Marmelo, Zélia Silva, Daniel Bolotas, Ricardo N. Alves, Paula A. Videira, et al.
Journal of Visualized Experiments, 2025
A new method for endocrine disruptor assessment in chicken fat tissues by LC-MS/MS analysis
M. João Nunes, Edgar Teixeira, João Paulo Noronha, Luis C. Branco, Mário S. Diniz
Analyst, 2025
Climate Change Impacts on Coastal Biological Communities
Juan M. Molina, Gabriela E. Blasina, Henrique N. Cabral, Diana Madeira, Mário Diniz, et al.
Marine Ecology an Ecosystemic View of Anthropogenic Impacts Volume 2, 2025
Peracetic acid treatment for removing steroid hormones in a pilot-scale wastewater installation
Rita Dias, Diogo Sousa, Pedro N. Carvalho, Vaidotas Kisielius, Mário Diniz, et al.
Journal of Water Process Engineering, 2024
The Impact of Perfluorooctanoic Acid (PFOA) on the Mussel Mytilus galloprovincialis: A Multi-Biomarker Evaluation
Sandra Copeto, Sara Ganço, Inês João Ferreira, Didier Sanchez, Maria João Nunes, et al.
Oceans, 2024
Fermented Cashew Nut Cheese Alternative Supplemented with Chondrus crispus and Porphyra sp.
Bruno M. Campos, Bruno Moreira-Leite, Abigail Salgado, Edgar Ramalho, Isa Marmelo, et al.
Applied Sciences Switzerland, 2024
Assessment of oxidative stress biomarkers in Palaemon varians exposed to deep eutectic systems
Mª Pilar Garralaga, Ines Ferreira, Laura Lomba, Elisabet Pires, Sara Gracia-Barberán, et al.
Environmental Science and Pollution Research, 2024
Integrated Multi-Biomarker Responses of Juvenile Zebra Seabream (Diplodus cervinus) to Warming and Acidification Conditions
Marta Dias, Pedro Pousão-Ferreira, Mário S. Diniz, António Marques, Rui Rosa, et al.
Oceans, 2024
Eco-innovative aquafeeds biofortified with Asparagopsis taxiformis to improve the resilience of farmed white seabream (Diplodus sargus) to marine heatwave events
Isa Marmelo, Cátia Lourenço-Marques, Iris A.L. Silva, Florbela Soares, Pedro Pousão-Ferreira, et al.
Heliyon, 2024
Asparagopsis taxiformis as a Novel Antioxidant Ingredient for Climate-Smart Aquaculture: Antioxidant, Metabolic and Digestive Modulation in Juvenile White Seabream (Diplodus sargus) Exposed to a Marine Heatwave
Alícia Pereira, Isa Marmelo, Marta Dias, Ana Catarina Silva, Ana Catarina Grade, et al.
Antioxidants, 2024

RECENT SCHOLAR PUBLICATIONS

Toxicopathology and expression of a CYP1-like gene in gills and digestive gland of wild Mediterranean mussel (Mytilus galloprovincialis) from sites with different contamination …
JD Vitorino, AP Rodrigo, M Loureiro, MS Diniz, PM Costa
Marine Pollution Bulletin 230, 119856 , 2026
2026
Do not despise the little ones: population-level responses of common gobies to seasonality along latitudinal cline show biased vulnerability of southern populations
C Madeira, M Missionário, D Madeira, C António, AM Rodrigues, ...
Marine Environmental Research 217, 107927 , 2026
2026
Evidence of Endocrine Disruption and Oxidative Stress in Mytilus galloprovincialis Exposed to 17α-Ethinylestradiol
S Copeto, IJ Ferreira, C Mansilha, A Melo, C Motta, M Silva, M Diniz
Journal of Marine Science and Engineering 14 (9), 829 , 2026
2026
Low oxidative stress of cephalopod early life stages under chronic and intermittent hypoxia
M Court, M Macau, T Marquês, T Repolho, M Diniz, VM Lopes, R Rosa, ...
Marine Biology 173 (2), 37 , 2026
2026
Laminaria ochroleuca as a natural remediator of copper-based antifouling agents in aquaculture systems
R Cereja, A Pereira, AC Brito, C Pires, M Diniz, I Ferreira, M Mil-Homens, ...
Marine Pollution Bulletin 223, 119023 , 2026
2026
Interactive Effects of Laminaria digitata Supplementation and Heatwave Events on Farmed Gilthead Seabream Antioxidant Status, Digestive Activity, and Lipid …
RVC Gomes, I Marmelo, T Chainho, A Pereira, D Bolotas, M Barata, ...
Environments 13 (1), 25 , 2026
2026
Citations: 2
Screening pollution biomarkers and burrowing population profiles in an infaunal key species from port estuarine areas: What does deeper sediment say?
M Díaz-Jaramillo, ML Ballesteros, G Chiang, CN Pegoraro, M Diniz
Marine Pollution Bulletin 222, 118773 , 2026
2026
Citations: 1
Transcriptomic response of Mytilus galloprovincialis to emerging contaminants: Data analysis workflow
S Copeto, I Ferreira, S Duarte, L Vieira, J Ferrão, M Silva, M Diniz, C Motta
2025
3.1 Transcriptomic analysis of the mussel Mytilus gallo-provincialis exposed to environmental contaminants
S Copeto, IJ Ferreira, S Duarte, L Vieira, J Ferrão, M Silva, M Diniz, ...
From Biomarkers to Transcriptomics: Assessing the Impact of PFOA, TBBPA, and … , 2025
2025
2.3 Oxidative stress biomarker responses to 17α-ethi-nylestradiol (EE2) exposure in the mussel Mytilus gal
S Copeto, IJ Ferreira, C Mansilha, A Melo, C Motta, M Silva, M Diniz
From Biomarkers to Transcriptomics: Assessing the Impact of PFOA, TBBPA, and … , 2025
2025
Napyradiomycin-based coatings: Promising eco-friendly, sustainable and circular solutions for effective marine antibiofouling
I Pereira, H Macedo, I Ferreira, B Gonçalves, S Piçarra, RG Sobral, ...
Environmental Technology & Innovation 39, 104248 , 2025
2025
Citations: 2
Development of Innovative Mediterranean-Style Semi-Hard Goat’s Cheese Supplemented with Seaweeds ( Palmaria palmata and Ulva sp.) and Its Characterization
BM Campos, BS Moreira-Leite, A Salgado, E Ramalho, I Marmelo, ...
Applied Sciences 15 (15), 8232 , 2025
2025
Citations: 1
Amphipods responses to ocean warming: sex-specific thermal tolerance, reproductive output and metabolomic profiles in males and females
JF Fernandes, L Feugère, L Marques, R Calado, MS Diniz, P Calosi, ...
Research summit 2025-Book of abstracts, 127 , 2025
2025
Asparagopsis taxiformis Feed Supplementation as a Tool to Improve the Resilience of Farmed Diplodus sargus to Marine Heatwave Events—A Metabolomics …
M Dias, I Marmelo, C António, AM Rodrigues, A Marques, MS Diniz, ...
Fishes 10 (7), 350 , 2025
2025
Citations: 1
Improving Farmed Juvenile Gilthead Seabream ( Sparus aurata ) Stress Response to Marine Heatwaves and Vibriosis Through Seaweed-Based Dietary Modulation
A Pereira, I Marmelo, T Chainho, D Bolotas, M Dias, R Cereja, M Barata, ...
Animals 15 (13), 1970 , 2025
2025
Citations: 2
Laminaria digitata Supplementation as a Climate-Smart Strategy to Counteract the Interactive Effects of Marine Heatwaves and Disease Outbreaks in Farmed Gilthead …
I Marmelo, T Chainho, D Bolotas, A Pereira, B Özkan, C Marques, ...
Environments 12 (7), 226 , 2025
2025
Citations: 2
Molecular landscape of therapeutic deep eutectic systems selective toxicity towards colorectal cancer
F Oliveira, J Pinto, F Amaro, J Pereira, I Ferreira, MS Diniz, PG de Pinho, ...
Sustainable Chemistry and Pharmacy 45, 102037 , 2025
2025
Citations: 1
What Type of Compounds are Expected in Halophyte Plants? Do They Have Potential Benefits for Human Consumption?
IJ Ferreira, ARC Duarte, M Diniz, R Salgado
International Conference on Water Energy Food and Sustainability, 145-153 , 2025
2025
Isolation, Fixation and Characterization of Juvenile Gilthead Seabream Head Kidney Leukocytes by Flow Cytometry
I Marmelo, Z Silva, D Bolotas, RN Alves, PA Videira, A Marques, MS Diniz, ...
JoVE (Journal of Visualized Experiments), e67978 , 2025
2025
Citations: 1
Antioxidant enzymes activities in Mytilus galloprovincialis exposed to 17α-ethinylestradiol (ee2): potential implications for human health
S Copeto, IJ Ferreira, C Motta, M Silva, M Diniz
2025

MOST CITED SCHOLAR PUBLICATIONS

Influence of temperature in thermal and oxidative stress responses in estuarine fish
D Madeira, L Narciso, HN Cabral, C Vinagre, MS Diniz
Comparative Biochemistry and Physiology Part A: Molecular & Integrative … , 2013
2013
Citations: 461
Effect of temperature on oxidative stress in fish: Lipid peroxidation and catalase activity in the muscle of juvenile seabass, Dicentrarchus labrax
C Vinagre, D Madeira, L Narciso, HN Cabral, M Diniz
Ecological indicators 23, 274-279 , 2012
2012
Citations: 400
Vulnerability to climate warming and acclimation capacity of tropical and temperate coastal organisms
C Vinagre, I Leal, V Mendonça, D Madeira, L Narciso, MS Diniz, ...
Ecological indicators 62, 317-327 , 2016
2016
Citations: 226
Histological biomarkers in liver and gills of juvenile Solea senegalensis exposed to contaminated estuarine sediments: A weighted indices approach
PM Costa, MS Diniz, S Caeiro, J Lobo, M Martins, AM Ferreira, M Caetano, ...
Aquatic toxicology 92 (3), 202-212 , 2009
2009
Citations: 224
Ecotoxicity of ketoprofen, diclofenac, atenolol and their photolysis byproducts in zebrafish (Danio rerio)
MS Diniz, R Salgado, VJ Pereira, G Carvalho, A Oehmen, MAM Reis, ...
Science of the Total Environment 505, 282-289 , 2015
2015
Citations: 174
Are fish in hot water? Effects of warming on oxidative stress metabolism in the commercial species Sparus aurata
D Madeira, C Vinagre, MS Diniz
Ecological Indicators 63, 324-331 , 2016
2016
Citations: 168
Ocean warming enhances malformations, premature hatching, metabolic suppression and oxidative stress in the early life stages of a keystone squid
R Rosa, MS Pimentel, J Boavida-Portugal, T Teixeira, K Truebenbach, ...
PLoS One 7 (6), e38282 , 2012
2012
Citations: 138
Oxidative stress and digestive enzyme activity of flatfish larvae in a changing ocean
MS Pimentel, F Faleiro, M Diniz, J Machado, P Pousão-Ferreira, MA Peck, ...
PLoS One 10 (7), e0134082 , 2015
2015
Citations: 137
Overview on modern approaches to speed up protein identification workflows relying on enzymatic cleavage and mass spectrometry-based techniques
JL Capelo, R Carreira, M Diniz, L Fernandes, M Galesio, C Lodeiro, ...
Analytica chimica acta 650 (2), 151-159 , 2009
2009
Citations: 135
Gold-nanobeacons for gene therapy: evaluation of genotoxicity, cell toxicity and proteome profiling analysis
J Conde, M Larguinho, A Cordeiro, LR Raposo, PM Costa, S Santos, ...
Nanotoxicology 8 (5), 521-532 , 2014
2014
Citations: 134
Oxidative stress and histological changes following exposure to diamond nanoparticles in the freshwater Asian clam Corbicula fluminea (Müller, 1774)
A Cid, A Picado, JB Correia, R Chaves, H Silva, J Caldeira, APA de Matos, ...
Journal of hazardous materials 284, 27-34 , 2015
2015
Citations: 118
Effect of increasing temperature in the differential activity of oxidative stress biomarkers in various tissues of the Rock goby, Gobius paganellus
C Vinagre, D Madeira, V Mendonça, M Dias, J Roma, MS Diniz
Marine environmental research 97, 10-14 , 2014
2014
Citations: 111
Biological treatment of the effluent from a bleached kraft pulp mill using basidiomycete and zygomycete fungi
AC Freitas, F Ferreira, AM Costa, R Pereira, SC Antunes, F Gonçalves, ...
Science of the total environment 407 (10), 3282-3289 , 2009
2009
Citations: 109
Ecophysiological responses of juvenile seabass (Dicentrarchus labrax) exposed to warming and dietary methylmercury
AL Maulvault, V Barbosa, R Alves, A Custódio, P Anacleto, T Repolho, ...
Science of the Total Environment 586, 551-558 , 2017
2017
Citations: 102
Single and combined effects of aluminum (Al 2 O 3 ) and zinc (ZnO) oxide nanoparticles in a freshwater fish, Carassius auratus
M Benavides, J Fernández-Lodeiro, P Coelho, C Lodeiro, MS Diniz
Environmental science and pollution research 23 (24), 24578-24591 , 2016
2016
Citations: 102
Developmental and physiological challenges of octopus ( Octopus vulgaris ) early life stages under ocean warming
T Repolho, M Baptista, MS Pimentel, G Dionísio, K Trübenbach, ...
Journal of Comparative Physiology B 184 (1), 55-64 , 2014
2014
Citations: 102
Umami taste in edible seaweeds: The current comprehension and perception
J Milinovic, P Mata, M Diniz, JP Noronha
International Journal of Gastronomy and Food Science 23, 100301 , 2021
2021
Citations: 101
Bioaccumulation and elimination of mercury in juvenile seabass (Dicentrarchus labrax) in a warmer environment
AL Maulvault, A Custodio, P Anacleto, T Repolho, P Pousão, ML Nunes, ...
Environmental Research 149, 77-85 , 2016
2016
Citations: 93
Estrogenic effects in crucian carp (Carassius carassius) exposed to treated sewage effluent
MS Diniz, I Peres, I Magalhaes-Antoine, J Falla, JC Pihan
Ecotoxicology and environmental safety 62 (3), 427-435 , 2005
2005
Citations: 91
Neuro-oxidative damage and aerobic potential loss of sharks under elevated CO 2 and warming
R Rosa, J Ricardo Paula, E Sampaio, M Pimentel, AR Lopes, M Baptista, ...
Marine Biology 163 (5), 119 , 2016
2016
Citations: 90

Mario Sousa Diniz

EDUCATION

RESEARCH INTERESTS

Scopus Publications

RECENT SCHOLAR PUBLICATIONS

MOST CITED SCHOLAR PUBLICATIONS