Irfan Pervaiz
@uchenab.edu.pk
Assistant Professor-Pharmacy
University of Chenab Gujrat
Dr. Irfan Pervaiz has been working at The Pharmacy Department, University of Chenab for past 4 years. During this period, he has taught various Pharmaceutical Chemistry courses including Medicinal Chemistry, Pharmaceutical Analysis, Organic Chemistry and Biochemistry. Irfan Pervaiz attended and graduated cum laude from College of Pharmacy, University of Punjab, Lahore as a Doctor of Pharmacy. Afterwards, he was accepted into MPhil Pharmaceutical Chemistry program at Faculty of Pharmacy & Alternative Medicine, Islamia University of Bahawalpur. He completed his PhD in the same discipline from the same department. In his thesis, Pervaiz investigated two medicinal plants namely, Calligonum polygonoides and Crateva adansonii for hitherto unexplored medicinal activities. Twenty Compounds were isolated and characterized from both plants. These molecules were also repositioned to exhibit high activity against urease, carbonic anhydrase, xanthine oxidase, α-glucosidase and Lipoxygenase.
EDUCATION
2019
PhD (Pharmaceutical chemistry), Islamia University of Bahawalpur, Pakistan
Thesis: Phytochemical and Biological Studies of Calligonum polygonoides and Crateva adansonii
2012
MPhil (Pharmaceutical chemistry), Islamia University of Bahawalpur, Pakistan
Thesis: Microbial Biotransformation of Antihyperlipidemic Drug
2009
Pharm.D, University of Punjab, Lahore
RESEARCH INTERESTS
Discovery, structure elucidation and identification of mode of action of natural and natural based products, advancement and repurposing of natural products as potential antimicrobial and anticancer agents.
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Abdulhakeem S. Alamri, Hammad Saleem, Irfan Pervaiz, Umair Khurshid, Juwairiya Butt, Tahir Ali Chohan, Majid Alhomrani, Marcello Locatelli, Syafiq Asnawi Zainal Abidin, Yasir Nasser H Alqahtani,et al.
Elsevier BV
Javed Ahmed, Mohsin Abbas Khan, Muhammad Ehsan Khalid, Irshad Ahmad, Irfan Pervaiz, Umair Khurshid, Saharish Khaliq, Kashif ur Rehman Khan, Muhammad Adeel Arshad, Ghadeer M. Albadrani,et al.
MDPI AG
The current study was designed to synthesize, characterize, and screen the molecular and biological activities of different metformin derivatives that possess potent antidiabetic potential with minimal side-effects. Metformin-based derivatives containing the metal complexes Cu II (MCu1–MCu9) and Zn II (MZn1–MZn9) were generated using aromatic aldehydes and ketones in a template process. The novel metal complexes were characterized through elemental analysis, physical state, melting point, physical appearance, Fourier-transform infrared (FTIR) spectroscopy, UV/visible (UV/Vis) spectroscopy, 1H nuclear magnetic resonance (NMR) spectroscopy, and 13C-NMR spectroscopy. Screening for inhibitory activity against the enzymes α-amylase and α-glucosidase, and molecular simulations performed in Schrödinger were used to assess the synthesized derivatives’ biological potential. Met1, Met2, Met3, and Met8 all displayed activities that were on par with the reference in an enzymatic inhibition assay (amylase and glucosidase). The enzyme inhibition assay was corroborated by molecular simulation studies, which also revealed a competitive docking score compared to the gold standard. The Swiss ADME online web server was utilized to compute ADME properties of metformin analogues. Lipinski’s rule of five held true across all derivatives, making it possible to determine the percentage of absorption. Metformin derivatives showed significant antidiabetic activities against both targeted enzymes, and the results of this work suggest that these compounds could serve as lead molecules for future study and development.
Waqas Mahmood, Irshad Ahmad, Mohsin Abbas Khan, Syed Adnan Ali Shah, Muhammad Ashraf, Mirza Imran Shahzad, Irfan Pervaiz, Muhammad Sajid-ur-Rehman, and Umair Khurshid
Elsevier BV
Marya Aziz, Saeed Ahmad, Umair Khurshid, Irfan Pervaiz, Arslan Hussain Lodhi, Nasrullah Jan, Sameera Khurshid, Muhammad Adeel Arshad, Mohamed M. Ibrahim, Gaber A. M. Mersal,et al.
MDPI AG
Plants of the genus Strobilanthes have notable use in folklore medicines as well as being used for pharmacological purposes. The present work explored the biological predispositions of Strobilanthes glutinosus and attempted to accomplish a comprehensive chemical profile through GC-MS of different fractions concerning polarity (chloroform and n-butanol) and LC-ESI-MS of methanolic extract by both positive and negative ionization modes. The biological characteristics such as antioxidant potential were assessed by applying six different methods. The potential for clinically relevant enzyme (α-amylase, α-glucosidase, and tyrosinase) inhibition was examined. The DPPH, ABTS, CUPRAC, and FRAP results revealed that the methanol fraction presented efficient results. The phosphomolybdenum assay revealed that the n-hexane fraction showed the most efficient results, while maximum metal chelation potential was observed for the chloroform fraction. The GC-MS profiling of n-butanol and chloroform fractions revealed the existence of several (110) important compounds presenting different classes (fatty acids, phenols, alkanes, monoterpenes, diterpenes, sesquiterpenoids, and sterols), while LC-ESI-MS tentatively identified the presence of 44 clinically important secondary metabolites. The n-hexane fraction exhibited the highest potential against α-amylase (497.98 mm ACAE/g extract) and α-glucosidase (605.85 mm ACAE/g extract). Significant inhibitory activity against tyrosinase enzyme was displayed by fraction. Six of the prevailing compounds from the GC-MS study (lupeol, beta-amyrin, stigmasterol, gamma sitosterol, 9,12-octadecadienoic acid, and n-hexadecanoic acid) were modelled against α-glucosidase and α-amylase enzymes along with a comparison of binding affinity to standard acarbose, while three compounds identified through LC-ESI-MS were docked to the mushroom tyrosinase enzyme and presented with significant biding affinities. Thus, it is assumed that S. glutinosus demonstrated effective antioxidant and enzyme inhibition prospects with effective bioactive molecules, potentially opening the door to a new application in the field of medicine.
Sirajudheen Anwar, Muhammad Faisal Nadeem, Irfan Pervaiz, Umair Khurshid, Nimra Akmal, Khurram Aamir, Muhammad Haseeb ur Rehman, Khaled Almansour, Farhan Alshammari, Mohd Farooq Shaikh,et al.
Frontiers Media SA
This study was designed to seek the phytochemical analysis, antioxidant, enzyme inhibition, and toxicity potentials of methanol and dichloromethane (DCM) extracts of aerial and root parts of Crotalaria burhia. Total bioactive content, high-performance liquid chromatography-photodiode array detector (HPLC-PDA) polyphenolic quantification, and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis were utilized to evaluate the phytochemical composition. Antioxidant [including 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH)], 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power assay (FRAP), cupric reducing antioxidant capacity CUPRAC, phosphomolybdenum, and metal chelation assays] and enzyme inhibition [against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glucosidase, α-amylase, and tyrosinase] assays were carried out for biological evaluation. The cytotoxicity was tested against MCF-7 and MDA-MB-231 breast cell lines. The root-methanol extract contained the highest levels of phenolics (37.69 mg gallic acid equivalent/g extract) and flavonoids (83.0 mg quercetin equivalent/g extract) contents, and was also the most active for DPPH (50.04 mg Trolox equivalent/g extract) and CUPRAC (139.96 mg Trolox equivalent /g extract) antioxidant assays. Likewise, the aerial-methanol extract exhibited maximum activity for ABTS (94.05 mg Trolox equivalent/g extract) and FRAP (64.23 mg Trolox equivalent/g extract) assays. The aerial-DCM extract was noted to be a convincing cholinesterase (AChE; 4.01 and BChE; 4.28 mg galantamine equivalent/g extract), and α-glucosidase inhibitor (1.92 mmol acarbose equivalent/g extract). All of the extracts exhibited weak to modest toxicity against the tested cell lines. A considerable quantities of gallic acid, catechin, 4-OH benzoic acid, syringic acid, vanillic acid, 3-OH-4-MeO benzaldehyde, epicatechin, p-coumaric acid, rutin, naringenin, and carvacrol were quantified via HPLC-PDA analysis. UHPLC-MS analysis of methanolic extracts from roots and aerial parts revealed the tentative identification of important phytoconstituents such as polyphenols, saponins, flavonoids, and glycoside derivatives. To conclude, this plant could be considered a promising source of origin for bioactive compounds with several therapeutic uses.
Umair Khurshid, Saeed Ahmad, Hammad Saleem, Arslan Hussain LodhI, Irfan Pervaiz, Mohsin Abbas Khan, Haroon Khan, Abdulwahab AlamrI, Mukhtar AnsarI, Marcello LocatellI,et al.
MDPI AG
This work was undertaken to explore the phytochemical composition, antioxidant, and enzyme-inhibiting properties of Neurada procumbens L. extracts/fractions of varying polarity (methanol extract and its fractions including n-hexane, chloroform, n-butanol, and aqueous fractions). A preliminary phytochemical study of all extracts/fractions, HPLC-PDA polyphenolic quantification, and GC-MS analysis of the n-hexane fraction were used to identify the phytochemical makeup. Antioxidant (DPPH), enzyme inhibition (against xanthine oxidase, carbonic anhydrase, and urease enzymes), and antibacterial activities against seven bacterial strains were performed for biological investigation. The GC-MS analysis revealed the tentative identification of 22 distinct phytochemicals in the n-hexane fraction, the majority of which belonged to the phenol, flavonoid, sesquiterpenoid, terpene, fatty acid, sterol, and triterpenoid classes of secondary metabolites. HPLC-PDA analysis quantified syringic acid, 3-OH benzoic acid, t-ferullic acid, naringin, and epicatechin in a significant amount. All of the studied extracts/fractions displayed significant antioxidant capability, with methanol extract exhibiting the highest radical-scavenging activity, as measured by an inhibitory percentage of 81.4 ± 0.7 and an IC50 value of 1.3 ± 0.3. For enzyme inhibition experiments, the n-hexane fraction was shown to be highly potent against xanthine oxidase and urease enzymes, with respective IC50 values of 2.3 ± 0.5 and 1.1 ± 0.4 mg/mL. Similarly, the methanol extract demonstrated the strongest activity against the carbonic anhydrase enzyme, with an IC50 value of 2.2 ± 0.4 mg/mL. Moreover, all the studied extracts/fractions presented moderate antibacterial potential against seven bacterial strains. Molecular docking of the five molecules β-amyrin, campesterol, ergosta-4,6,22-trien-3β-ol, stigmasterol, and caryophyllene revealed the interaction of these ligands with the investigated enzyme (xanthine oxidase). The results of the present study suggested that the N. procumbens plant may be evaluated as a possible source of bioactive compounds with multifunctional therapeutic applications.
Irfan Pervaiz, Hammad Saleem, Muhammad Sarfraz, Muhammad Imran Tousif, Umair Khurshid, Saeed Ahmad, Gokhan Zengin, Kouadio Ibrahime Sinan, Marcello Locatelli, Fawzi M. Mahomoodally,et al.
Elsevier BV
Shabana Anwar, Mohsin Abbas Khan, Irshad Ahmed, Irfan Pervaiz, and Hamid Saeed Shah
Bentham Science Publishers Ltd.
Background: The use of antibiotics has compromised due to the appearance of multidrug- resistant strains and decreased susceptibility of strains to antibiotics. Antibiotic resistance has become a worldwide threat as well as Helicobacter pylori induced gastric cancer is a major problem nowadays. Therefore, it is the need of time to synthesize potent anti-urease motifs. Introduction: Schiff bases represent a large class of biologically active compounds that exhibited a broad spectrum of biological activities. Amikacin is an important drug used against multidrugresistant species of microbes. Therefore, imine derivatives of amikacin may help to reduce the resistance of microbes and to treat the Helicobacter pylori induced stomach problems by inhibiting the Helicobacter Pylori Urease enzyme. Methods: Schiff bases of amikacin were synthesized and screened for in-vitro antibacterial assay performed by well diffusion method against Bacillus megaterium (Bm), Bacillus subtilis (Bs), Stenotrophomonas maltophilia (Sm), Staphylococcus aureus (Sa), Micrococcus luteus (Ml), Serratia marcescens (S. mar), Escherichia coli (E. coli) and anti-urease activity was performed by Indophenol method. Structures confirmed by IR, 1HNMR and 13CNMR spectroscopy. Results: Compounds showed varying degrees of antibacterial effects. Schiff bases A2 and A8 exhibited potent urease inhibition activity with Ki values 0.2754 and 0.2908 μM, respectively and their binding affinity was calculated as greater than the standard drug. Conclusion: Derivatives of amikacin with aromatic rings were more active antibacterials than those with an aliphatic side chain. The potent anti-urease activity has been recorded for compounds A2 and A8. Therefore, they may serve as lead compounds in the discovery of Helicobacter pylori Urease inhibitors.
Irfan Pervaiz, Saeed Ahmad, Adeel Arshad, Umair Khurshid, and Abdul Basit
African Journals Online (AJOL)
Purpose: To determine the urease-inhibitory activity and chemical constituents of fractions of Calligonum polygonoides and Crateva adansonii separated by physical properties.
 Methods: The anti-urease activities of different fractions of the plants (methanol, n-hexane, CHCl3, nbutanol) were evaluated using a standard procedure. The chemical constituents of the extracts with the highest urease-inhibitory activity were determined by gas chromatography-mass spectrometry.
 Results: The n-hexane fractions of both plants had higher urease-inhibitory activity and a lower halfmaximal inhibitory concentration (IC50) than the other extracts. GC-MS evaluation revealed that nhexane fraction of C. polygonoides was rich in fatty acids (39.36 %), sterols (22.29 %), long chain alkanes (98.5 %), and a few volatiles (5.26 %), while the n-hexane fraction of C. adansonii had high levels of alkanes (35.03 %), sterols (10.46 %), fatty acid esters (46.82 %), and triterpenes (23.76 %).
 Conclusion: The n-hexane fractions of the plants demonstrate high urease-inhibitory activity. Thus, these plant-based anti-urease fractions can potentially serve as a starting point for the development of novel antibacterial agents with enhanced efficacy and reduced antibiotic resistance in the treatment of pathological conditions and infections associated with urease.
Irfan Pervaiz, Saeed Ahmad, Muhammad Fahad Mukhtar, Adeel Arshad, Muhammad Imran, and Wajahat Mahmood
Springer Science and Business Media LLC
Saeed Ahmad, Farhan Hameed Khaliq, Asadullah Madni, Muhammad Nabeel Shahid, and Irfan Pervaiz
FapUNIFESP (SciELO)
In the present research, the steroidal anti-asthmatic drug beclomethasone dipropionate was subjected to microbial biotransformation by Aspergillus niger. Beclomethasone dipropionate was transformed into various metabolites first time from microbial transformation. New drug metabolites produced can act as new potential drug molecules and can replace the old drugs in terms of safety, efficacy, and least resistance. They were purified by preparative thin layer chromatography technique, and their structures were elucidated using modern spectroscopic techniques, such as 13C NMR, 1H NMR, HMQC, HMQC, COSY, and NOESY, and mass spectrometry, such as EI-MS. Four metabolites were purified: (i) beclomethasone 17-monopropionate, (ii) beclomethasone 21-monopropionate, (iii) beclomethasone, and (iv) 9beta,11beta-epoxy-17,21-dihydroxy-16beta-methylpregna-1,4-diene-3,20-dione 21-propionate.
Irfan Pervaiz, Saeed Ahmad, Farhan Hameed Khaliq, Adeel Arshad, Muhammad Imran, Barkat Ali Khan, Aftab Ullah, Usman Ali, Kashif Iqbal, Muhammad Usman,et al.
Informa UK Limited
Structural transformation of ezetimibe was performed by fungi Beauvaria bassiana and Cunninghamella blakesleeana. The metabolites were identified by different spectroscopic techniques as (3R,4S)-1-(4-fluorophenyl)-3-((E)-3-(4-fluorophenyl) allyl)-4-(4-hydroxyphenyl) azetidin-2-one (2), (3R, 4S)-1-(4-fluorophenyl)-3-(3-(4fluorophenyl)-3-oxopropyl)-4-(4-hydroxyphenyl) azetidin-2-one (3), (3R,4S) 1-(4-fluorophenyl)-3-(3-(4-fluorophenyl) propyl)-4-(4-hydroxyphenyl) azetidin-2-one (4) and (2R,5S)-N, 5-bis (4-fluorophenyl)-5-hydroxy-2-(4-hydroxybenzyl) pentanamide (5). This study displays two important features of these fungi, viz., their ability to metabolize halogenated compounds, and their capacity to metabolize drugs that are targets of the UDP-Glucuronyl Transferase System, a phenomenon not commonly observed.
I. Pervaiz, S. Ahmad, M. A. Madni, H. Ahmad, and F. H. Khaliq
Pleiades Publishing Ltd
I. Pervaiz, S. Ahmad, M. A. Madni, H. Ahmad, and F. H. Khaliq
Akademizdatcenter Nauka
For centuries microbial biotransformation has proved to be an imperative tool in alleviating the production of various chemicals used in food, pharmaceutical, agrochemical and other industries. In the field of phar- maceutical research and development, biotransformation studies have been extensively applied to investigate the metabolism of compounds (leads, lead candidates, etc.) using animal models. The microbial biotransfor- mation phenomenon is then commonly employed in comparing metabolic pathways of drugs and scaling up the metabolites of interest discovered in these animal models for further pharmacological and toxicological evaluation. Microorganisms can conveniently afford drugs difficult obtained via synthesis. The plethora of reported microbial biotransformations along with its added benefits has already invoked further research in bioconversion of novel and structurally complex drugs. This review alternatively discusses the prospect of microbial biotransformation studies as a significant element ameliorating drug discovery and design in terms of cost-effectiveness, environment protection and greater structural diversity as compared to animal models used to study metabolism. To explicate the microbial biotransformation paradigm in drug designing 3 main areas in this aspect have been analyzed: 1--lead expansion: obtaining pharmacologically improved metabo- lites from bioactive molecules; 2--biosynthesis of precursors/intermediates involved in the production of bioactive molecules; 3--resolution of racemic mixture to obtain enantiomers possessing different pharma- cological profiles.