Bashar Mudhaffar Abdullah
@ruc.edu.iq
Department of medical laboratories
Alrafidain university college
EDUCATION
PhD in Clinical Biochemistry
RESEARCH INTERESTS
Biochemistry, Clinical biochemistry, organic chemistry
Scopus Publications
Scopus Publications
Ebraheem Abdu Musad Saleh, Bashar Mudhaffar Abdullah, Hala Kh. Mohammed, Asmaa F. Kassem, I. B. Sapaev, Pallavi Ghildiyal, Abdul-hameed. M. Hamoody, Ahmed Alawadi, Usama S. Altimari, and Maryam Sadat Ghorayshi Nejad
Springer Science and Business Media LLC
Nava Yugavathy, Bashar Mudhaffar Abdullah, Soo Kun Lim, Abdul Halim Bin Abdul Gafor, Muh Geot Wong, Sunita Bavanandan, Hin Seng Wong, and Hasniza Zaman Huri
MDPI AG
The study of anaemia is a well-developed discipline where the concepts of precision medicine have, in part, been researched extensively. This review discusses the treatment of erythropoietin (EPO) deficiency anaemia and resistance in cases of chronic kidney disease (CKD). Traditionally, erythropoietin-stimulating agents (ESAs) and iron supplementation have been used to manage anaemia in cases of CKD. However, these treatments pose potential risks, including cardiovascular and thromboembolic events. Newer treatments have emerged to address these risks, such as slow-release and low-dosage intravenous iron, oral iron supplementation, and erythropoietin–iron combination therapy. Another novel approach is the use of hypoxia-inducible factor prolyl hydroxylase inhibitors (HIF-PHIs). This review highlights the need for precision medicine targeting the genetic components of EPO deficiency anaemia in CKD and discusses individual variability in genes such as the erythropoietin gene (EPO), the interleukin-β gene (IL-β), and the hypoxia-inducible factor gene (HIF). Pharmacogenetic testing aims to provide targeted therapies and interventions that are tailored to the specific characteristics of an individual, thus optimising treatment outcomes and minimising resistance and adverse effects. This article concludes by suggesting that receptor modification has the potential to revolutionise the treatment outcomes of patients with erythropoietin deficiency anaemia through the integration of the mentioned approach.
Sangeetha Perumalsamy, Hasniza Zaman Huri, Bashar Mudhaffar Abdullah, Othman Mazlan, Wan Azman Wan Ahmad, and Shireene Ratna D. B. Vethakkan
MDPI AG
Type 2 diabetes mellitus (T2DM) is characterized by impaired insulin secretion on a background of insulin resistance (IR). IR and T2DM are associated with atherosclerotic coronary artery disease (CAD). The mechanisms of IR and atherosclerosis are known to share similar genetic and environmental roots. Endothelial dysfunction (ED) detected at the earliest stages of IR might be the origin of atherosclerosis progression. ED influences the secretion of pro-inflammatory cytokines and their encoding genes. The genes and their single nucleotide polymorphisms (SNPs) act as potential genetic markers of IR and atherosclerosis. This review focuses on the link between IR, T2DM, atherosclerosis, CAD, and the potential genetic markers CHI3L1, CD36, LEPR, RETN, IL-18, RBP-4, and RARRES2 genes.
Riyadh Hasan Mohammed Ali, Bashar Mudhaffar Abdullah, and Rasha Ismail Ahmed
Universitas Gadjah Mada
This study aims to estimate a simple, rapid and sensitive method for a trace amount of atropine (ATR) in medicinal compounds. Two approaches were followed to accomplish this aim, i.e., spectrophotometric determination of pure ATR and pharmaceutical preparations using SbI42− ion as a new reagent. The procedure involves the implementation of an ion-association complex with this alkaloid. The resulting complex was extracted and detected spectrophotometrically at 492 nm. Appropriate parameters were investigated, including the ion SbI42− concentration and the pH value of the complex formation. Using chloroform to extract the complex, taking into consideration extraction time and volume of solvent used. The calibration graph is linear in the ranges of 0.5–5.0 × 10−3 M. Precision, accuracy, detection limit, and RSD %, as well as relative standard deviation (n = 5), were calculated. The test sensitivity was 0.013 µg cm−2. Several interference additives were studied by investigating the effect of equal and duplicate quantities of some common excipients on selectivity, such as starch, glucose, lactose, glycerin, and talc. The molar ratio of the SbI42−_ATR was determined. The amount of ATR in the pharmaceutical tablets and eye drop preparation was calculated using Erel at ratios of 2.24 and 2.75%, respectively.
Nor Razida Razali, Hasniza Zaman Huri, Luqman Ibrahim, Shireene Ratna Vethakkan, and Bashar Mudhaffar Abdullah
FapUNIFESP (SciELO)
In clinical practice, simvastatin is usually used in the treatment of dyslipidemia patients and those at risk of or with established cardiovascular disease. However, previous studies have shown that simvastatin has the potential to affect glycemic parameters as it reportedly reduced insulin secretion and sensitivity. The exact mechanism by which simvastatin affects glycemia is still unknown, but previous studies have postulated the involvement of the glucose-insulin secretion mechanism. This review focuses on the effects of simvastatin, either alone or in combination with other lipid lowering agents, antidiabetics and antihypertensives, on glucose homeostasis. Some studies have reported that simvastatin might impair the levels of glucose metabolism markers in the blood while others have reported no effect or improvement in glycemia.
Nadia Salih, Jumat Salimon, Bashar Mudhaffar Abdullah, and Emad Yousif
Elsevier BV
Emad Yousif, Ahmed Majeed, Khulood Al-Sammarrae, Nadia Salih, Jumat Salimon, and Bashar Abdullah
Elsevier BV
Jumat Salimon, Bashar Mudhaffar Abdullah, and Nadia Salih
Elsevier BV
Emad Yousif, Raghad Haddad, Badrul Hisyam Yahya, Syazwan Aizad, Noraziani Zainal Abidin, Hazreen Omar, Ili Afiqa Ab Kadir, Hasniza Zaman Huri, Bashar Mudhaffar Abdullah, and Saiful Irwan Zubairi
Penerbit UTM Press
A complex of Fe(III) with 4-amino-5-(pyridyl)-4H-1,2,4-triazole-3-thiol was prepared and evaluated as a photodegradation for polyvinyl chloride (PVC). Polyvinyl chloride was dissolved in THF solvent (5%, w/w) and functionalized with Fe (III) complex to form PVC films by means of solvent-casting method with a thickness of 40 μm. To study the effectiveness of Fe (III) complex additive, different concentrations (0.01, 0.02, 0.03, 0.04 and 0.05 g) were introduced into the PVC solution. The films’ photodegradation was investigated; the photodegradation activity of the compounds was determined by monitoring the carbonyl group, weight loss method and morphological study with irradiation time. The obtained experimental results showed that the additive behaved successfully as a photoinducer for the degradation process for the PVC films and the photooxidation rate increased with the increase of additive concentration.
Nor Bahirah Kasim, Hasniza Zaman Huri, Shireene Ratna Vethakkan, Luqman Ibrahim, and Bashar Mudhaffar Abdullah
Future Medicine Ltd
Generally, obese and overweight individuals display higher free fatty acid levels, which stimulate insulin resistance. The combination of overweight or obesity with insulin resistance can trigger Type 2 diabetes mellitus (T2DM) and are primary contributing factors to the development of uncontrolled T2DM. Genetic polymorphisms also play an important role as they can impact a population's susceptibility to becoming overweight or obese and developing related chronic complications, such as uncontrolled T2DM. This review specifically examines the genetic polymorphisms associated with overweight and obesity in patients with uncontrolled T2DM. Particularly, gene polymorphisms in ADIPOQ (rs1501299 and rs17300539), LepR (rs1137101 and rs1045895), IRS2 (rs1805092), GRB14 (rs10195252 and rs3923113) and PPARG (rs1801282) have been associated with overweight and obesity in uncontrolled T2DM.
Bashar Mudhaffar Abdullah, Saiful Irwan Zubairi, Hasniza Zaman Huri, Nany Hairunisa, Emad Yousif, and Roma Choudhury Basu
Public Library of Science (PLoS)
Presently, plant oils which contain high percentage of linoleic acid 1 are perceived to be a viable alternative to mineral oil for biolubricant applications due to their biodegradability and technical properties. In order to get biodegradable lubricant, triester derivatives compounds (1–5) were synthesized and characterized. The processes involved were monoepoxidation of linoleic acid 2, oxirane ring opening 3, esterification 4 and acylation 5. The structures of the products were confirmed by FTIR, 1H and 13C-NMR and LC-MS. The results that showed lowest temperature properties were obtained for triester 5, with a pour point value (PP) of -73°C, highest onset temperature (260°C) and lowest volatility at 0.30%. Viscosity index (VI) increased for the ester’s synthetic compounds (2, 3, 4, 5), while the PP decreased. This behavior is the result of the increase of the chain length of the branching agents. Triester based linoleic acid has improved properties such as low-temperature and tribological properties. These results will make it feasible for plant oil to be used for biolubricants, fuels in chain saws, transmission oil and brake fluid.
Bashar Mudhaffar Abdullah, Rahimi M. Yusop, Jumat Salimon, Darfizzi Derawi, and Waled Abdo Ahmed
Penerbit Universiti Kebangsaan Malaysia (UKM Press)
Monoepoxidation linoleic acid (MEOA) has advantages for industrial applications. MEOA was synthesized using immobilized Candida antarctica lipase (Novozym 435). At optimum conditions, higher yield (82.14 %) and medium oxirane oxygen content, OOC (4.91 %) of MEOA were predicted at 15 μL of H2O2, 120 mg of Novozym 435 ® and 7 hours of reaction time. Fourier Transform Infrared Spectroscopy (FTIR) spectra of the MEOA showed monoepoxide group at 820 cm. H NMR analysis confirmed the monoepoxide group at 2.92 – 3.12 ppm while the monoepoxide signals of C NMR appear at 54.59 – 57.29 ppm. LC-MS analysis shows that of MEOA gives m/z at 296.22 as final product. MEOA exhibited good pour point (PP) of -41 oC. Flash point (FP) of MEOA increased to 128 oC comparing with 115 oC of linoleic acid (LA). In a similar fashion, viscosity index (VI) for LA was 224 generally several hundred centistokes (cSt) more viscous than MEOA 130.8. MEOA was screened to measure their oxidative stability (OT) which was observed at 168 oC. It is evident that increasing the hydrogen peroxide amount has a strong effect on the reaction kinetics; however, a large excess of hydrogen peroxide results in accumulation of peracid in the final product.
Krisna Veni Balakrishn, Bashar Mudhaffar Abdullah, Hasniza Zaman Huri, Nany Hairunisa, and Narazah Mohd Yusoff
Science Alert
Human immuno-deficiency virus (HIV) disease continues to be a serious health issue for parts of the world. Worldwide, there were about 2.5 million new cases of HIV reported in 2011. The HIV continues to be a major global public health issue with approximately 34 million people living with HIV in 2011. However, the development of anti-viral has blunted the AIDS epidemic in the western world but globally the epidemic has not been curtailed. Negative Regulatory Factor (Nef) is one of the accessory genes found only within HIV and SIV genomes and thought to play a key role in the progression to AIDS. Nef is considered as a potential anti viral target for preventing or at least delaying HIV pathogenesis. It suggesting that, the Nef protein that was detected not identically synthesis through post translation modification though it was expressed in the cytoplasm of HEK 293 cells. The ability of not expressing the targeted myristoylated 27 kDa Nef protein was to various unpreventable factors due to time limitation and lacking of skills in the filed cloning and cell culture.
Firas Hassan, Ayah Abdul Hameed, Ahemd Alshanon, Bashar Mudhaffar Abdullah, Hasniza Zaman Huri, Nany Hairunisa, and Emad Yousif
Science Alert
Multipara metric analysis of compound toxicity at the level of individual cells using flow cytometry and cellular imaging-based approaches such as High Content Screening (HCS) have played key roles in the detection of toxicity and classification of compounds based on observed patterns of reversible and irreversible cellular injury. Gold III complex (AuL2) of bidentate ligand derived from the cyclization reaction of Schiff base of 4-amino-5-phenyl-4h-1,2,4-triazole-3-thiol with thioglycolic acid was synthesized and characterized by using melting point, FTIR spectroscopy, HNMR, UV-Visible spectroscopy and elemental analysis. The presence of chloride counter ion in complex was supported by conductivity measurement and the presence of hydrated water was supported by thermal gravimetric studies. We examine the cytotoxic effects of gold complex and its ligand in one cultured cellular models (MCF7 cell line) by High Content Screening (HCS) and analysis and cell viability assay (MMT assay). The inhibitory effect of AuL2 on breast cancer cell growth was due to induction of apoptosis as evidenced by annexin V staining and cell shrinkage. The study found that AuL2-mediated lead to disruption of Mitochondrial Membrane Potential (MMP), cell membrane permeability, nuclear condensation, fragmentation and release of cytochrome c from the mitochondria into the cytosol and also suggesting AuL2 as a potential MCF7 inhibitor compared to doxorubicin as positive control. In this study, data showed that gold (III) complex AuL2 may have therapeutic value in breast cancer treatment worthy of further development.
Jumat Salimon, Bashar Mudhaffar Abdullah, Rahimi M Yusop, and Nadia Salih
Springer Science and Business Media LLC
Emad Yousif, Basim I. Mehdi, Rahimi Yusop, Jumat Salimon, Nadia Salih, and Bashar M. Abdullah
Informa UK Limited
Darfizzi Derawi, Bashar Mudhaffar Abdullah, Hasniza Zaman Huri, Rahimi M. Yusop, Jumat Salimon, Nany Hairunisa, and Nadia Salih
Hindawi Limited
Palm olein (POo) is widely produced as edible oil in tropical countries. POois considered as renewable raw material for the new industrial and pharmaceutical products synthesis based on its characterization. Palm olein was good on its viscosity index, oxidative stability, and flash and fire point. POocontained unsaturated triacylglycerols (TAGs): POO (33.3%); POP (29.6%) which plays an important role in chemical modification process to produce new industrial products. The double bond was detected on1H-NMR (5.3 ppm) and13C-NMR (130 ppm) spectra. The chemical compositions of POowere tested by using high performance liquid chromatography (HPLC) and gas chromatography (GC) techniques. This unsaturated oil is potentially to be used as renewable raw materials in chemical modification process to synthesise polyols, polyurethane, and biolubricant for industrial and pharmaceutical products application.
Tagreed K. Hamad, Rahimi M. Yusop, Wasan A. Al-Taa’y, Bashar Abdullah, and Emad Yousif
Hindawi Limited
The effect of continuous CO2laser radiation on the optical properties of pure polyvinyl chloride and doped of ZnO nanoparticles with two different concentrations (10, 15%) has been investigated. All samples were prepared using casting method at room temperature. Optical properties (absorption, transmission, absorption coefficient, extinction coefficient, refractive index, and optical conductivity) of all films after CO2laser irradiated have been studied as a function of the wavelength in the range (200–800) nm for three energies (300, 400 and 500 mJ). It has been found that the transmission, energy gap, and refractive index increase with increasing laser energy. The values of absorption, Urbach energy, absorption coefficient, extinction coefficient, and optical conductivity were decreased.
Bashar Mudhaffar Abdullah, Nadia Salih, and Jumat Salimon
Elsevier BV
Wasan Al-Taa’y, Mohammed Abdul Nabi, Rahimi M. Yusop, Emad Yousif, Bashar Mudhaffar Abdullah, Jumat Salimon, Nadia Salih, and Saiful Irwan Zubairi
Hindawi Limited
Optical properties of pure and doped poly(vinyl chloride) (PVC) films, prepared by using casting technique, with different nanosize zinc oxide (ZnO) concentrations (1–20) wt% have been studied. Parameters such as extinction coefficient, refractive index, real and imaginary parts, Urbach energy, optical conductivity, infinitely high frequency dielectric constant, and average refractive index were studied by using the absorbance and transmittance measurement from computerized UV-visible spectrophotometer (Shimadzu UV-1601 PC) in the spectral range 200–800 nm. This study reveals that the optical properties of PVC are affected by the doping of ZnO where the absorption increases and transmission decreases as ZnO concentration increases. The extinction coefficient, refractive index, real and imaginary parts, infinitely high frequency dielectric constant, and average refractive index values were found to increase with increasing impurity percentage. The Urbach energy values are found to be decreasing with increasing ZnO concentration. The optical conductivity increased with photon energy after being doped and with the increase of ZnO concentration.
Nadia Salih, Jumat Salimon, Emad Yousif, and Bashar Mudhaffar Abdullah
Springer Science and Business Media LLC