Dr. Amit Ravindra Nisal
@mcpune.bharatividyapeeth.edu
Associate Professor in Pathology
Bharati Vidyapeeth (Deemed To Be) University Medical College Hospital and Research Centre, Pune
Trainings and Work Experience:
1. Worked as Tutor in Pathology at KEM Hospital, Pune and trained for bone marrow and special hematology techniques under the guidance of Dr. Rumma Manchanda; eminent haematopathologist from Pune in KEM Hospital as well as in her private laboratory for about 10 years.
2. Worked as Junior Pathologist at Deenanath Mangeshkar Hospital, Pune for 2.5 years.
3. I am currently working as Associate Professor in Department of Pathology, Bharati Vidyapeeth (DTU) Medical College having 880 bedded super-specialty Hospital with undergraduate and postgraduate teaching programme.
4. I am involved in routine as well as special haematology reporting in the laboratory with special interest in bone marrow procedure and reporting, flow cytometry and HPLC.
5. NABL ISO 15189:2012 Four days of internal auditor course at Bharati Hospital from 22-25th June 2016.
6. I am currently in charge of Haematology Labs and course coordinator for Diagnostic Haematology fellowship.
EDUCATION
M.D. (Pathology)- Bharati Vidyapeeth (Deemed To Be) University Medical College Hospital and Research Centre, Pune (2015)
D.C.P. - Rural Medical College, Loni, University of Pune (2002)
Fellowship Diagnostic Haematology - Bharati Vidyapeeth (Deemed To Be) University Medical College Hospital and Research Centre, Pune (2019)
RESEARCH INTERESTS
To work in the area of Hematopathology with advance techniques and provide quality results to the patients and treating clinicians. Areas of interest are Neonatal hematology, Bone marrow, Flow cytometry, Coagulation and HPLC.
Scopus Publications
Scholar Citations
Scholar h-index
Scopus Publications
Rachana Lakhe, Amit Nisal, Preeti Doshi, and Ravindra Nimbargi
Universitas Nahdlatul Ulama Surabaya
Various coagulation tests like Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) are estimated by automated coagulation analyzers. The newer fully automated analyzers generate clot wave forms aPTT-CWA for these parameters are derived. In this study, the objective was to analyze clot wave form characteristics morphology and its first and second derivative values in cases with abnormal APTT. ACL TOP 300 generated curves for APTT in a total 125 patients with 20 normal controls are included. First derivative, second derivative, morphology of curve: sigmoid, biphasic, prolonged pre-coagulation phase, second derivative morphology like early and late shoulder, biphasic peak, delayed deceleration were the analyzed parameters. Wave clot forms of 125 patients were included in this study. Patients (M:F - 2.2:1, mean age: 46.9 ± 20 years). A spectrum of clinical conditions was Covid (20%), liver disease (23%), polytrauma (10.4%), cardiac diseases (8.8%), sepsis/DIC (7.2%), thromboembolism (7.2%), renal diseases (6.4%), bacterial infections (4%), dengue (4%), snake bite (1.6%) and factor deficiency (1.6%). Liver and heart disease showed a significant difference in acceleration and deceleration peaks followed by sepsis, dengue, polytrauma and sepsis/DIC. Deceleration peak was prolonged in patients of Covid (p<0.05). Sepsis and liver diseases showed prolonged first derivative peak (p<0.05). CWA is very easily available on all automated coagulation analyzers. It is inexpensive with fast turn round time. Both quantitative as well as qualitative informations such as velocity, acceleration of clot formation and wave pattern details were recorded. Our study highlights importance of quantitative and qualitative CWA parameters acquired by performing APTT test for the automated analyzers.
Amit Nisal, Vidya Kale, Anjali Kelkar, and Ravindra Nimbargi
IP Innovative Publication Pvt Ltd
Coagulopathy has a high prevalence among critically ill patients and is the result of the derangement of both procoagulant and anticoagulant components of the coagulation system. Liver and renal function tests are some of the most commonly performed blood tests which assess the liver and kidney injury and these are one of the commonest causes of deranged coagulation parameters in these patients. This prospective observational study was conducted for two years in Department of Pathology in a tertiary care hospital in university medical college in western India. 219 cases with underlying liver and renal disorders were included in this study. Complete blood counts and coagulation studies including Prothrombin time (PT), Activated Partial Thromboplastin time (APTT), Fibrinogen were done. Statistical analysis was done to evaluate the correlation between various parameters. In our study, hepatic encephalopathy was the commonest cause followed by alcoholic liver disease and liver cirrhosis. Chronic kidney disease was common cause in patients with renal disorders. Deranged Bilirubin levels had a significant statistical association with PT while APTT had a significant association with blood urea levels. Advanced diagnostic and laboratory methods, early recognition of the signs and symptoms of coagulopathy and the complicating factors in liver and renal disorders in these patients is possible today. Liver and renal disorders are one of the important underlying causes for development of coagulopathies in criticially ill patients. Prompt and correct identification of these disorders and associated coagulopathy is important for proper management and improving outcome in these patients.
Sandip Bartakke, Amit Nisal, Vibha Bafna, and Aashna Valecha
Springer Science and Business Media LLC
Anjali Kelkar, Amit Nisal, Aaishwarya Dhabe, Anu Christopher, and Ravindra Nimbargi
IP Innovative Publication Pvt Ltd
Introduction:Acquired immunodeficiency Syndrome (AIDS) is caused by Human Immunodeficiency virus leading to profound immunosuppression and its consequences. Depletion of CD4+ T cells is a hallmark of AIDS apart from anaemia, leucopenia and thrombocytopenia. Monitoring of CD4 count is crucial for an effective treatment. Absolute lymphocyte count (ALC) less than 1000/μl i.e. lymphopenia correlates with lower CD4 counts as per various studies and recommendation by the WHO. Detection of lymphopenia and measurement of ALC are routinely obtained parameters using a basic haematology analyser. This study was done to evaluate the role of absolute lymphocyte count (ALC) as an alternative to CD4 count in HIV positive patients. Materials and Methods: This prospective observational study was conducted for the period of two years in the department of Pathology of a tertiary care hospital in western India. 110 HIV positive patients consenting to be the part of the study were included. Complete blood counts (CBC), CD4 and CD8 counts were done for all patients following stringent quality control protocols. Statistical analysis was done to evaluate the correlation between various parameters. Results : 65.5% cases had lymphopenia with an absolute CD4 count <200cells/μ l. Using ROC curve, we found that ALC of less than 575/μl has a significant statistical association with CD4 count of less than 200/μl.Correlation of lymphopenia with CD4:CD8 ratio was not found to be statistically significant in our study. Conclusion : ALC can be considered as a cost effective alternative to absolute CD4 counts in the monitoring of HIV positive patients. © 2020 Published by Innovative Publication. This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/)