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CESPU - IUCS - UNIPRO
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Dentistry, General Dentistry, Oral Surgery, Education
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António Melo-Ferraz, Cristina Coelho, Paulo Miller, Maria Begoña Criado, and Maria Céu Monteiro
Springer Science and Business Media LLC
António Melo‐Ferraz, Paulo Miller, Maria Begoña Criado, Maria Céu Monteiro, and Cristina Coelho
Wiley
This study investigates the antimicrobial properties of leukocyte‐ and platelet‐rich fibrin (L‐PRF) against Enterococcus faecalis (ATCC 29212), Pseudomonas aeruginosa (ATCC 27853), and Candida albicans (ATCC 90028). Infections can hinder wound healing posing challenges. L‐PRF's potential for regeneration and antimicrobial action has been studied. Considering the increasing concern about antibiotic resistance, assessing the antimicrobial properties of L‐PRF provides valuable insights into its potential as a therapeutic agent in postoperative infections. Twenty volunteers were enrolled in the study, following ethical guidelines, and obtaining informed consent. Blood samples were collected and L‐PRF was prepared. Microbial suspensions were prepared, and susceptibility testing was performed using the Kirby‐Bauer agar diffusion method. The study revealed significant heterogeneity in the susceptibility to L‐PRF. All L‐PRF membrane samples exhibited antimicrobial activity against P. aeruginosa, with inhibition zones of 13 mm ± 3.85 SD. Enterococcus faecalis displayed inhibition diameter of 7.25 mm ± 5.15 SD. Candida albicans susceptibility to L‐PRF varied among samples, with both inhibitory and non‐inhibitory results. Results showed varying degrees of antimicrobial activity, particularly against P. aeruginosa, and highlight the complexity of the L‐PRF‐microorganism interaction. Further investigations are needed to elucidate the clinical implications and optimize the use of L‐PRF.
Júlio C. M. Souza, Valter Fernandes, Ana Correia, Paulo Miller, Oscar Carvalho, Filipe Silva, Mutlu Özcan, and Bruno Henriques
Springer Science and Business Media LLC
António Melo-Ferraz, Cristina Coelho, Paulo Miller, Maria Begoña Criado, and Maria Céu Monteiro
Springer Science and Business Media LLC