Gene Editing of Olfactory Receptors to Alter Host-Seeking Behaviour in Aedes aegypti Mosquitoes Anisha Chaudhary, Dr. Soham Mahendrabhai Trivedi, Dr. Sandeep Kumar Singh, Babu Lal Chaudhary, Dr.P. Prakash, Vivek Saraswat, Dr. Dharmsheel Shrivastava Journal of Animal Environment, 2026 Over 700,000 deaths are annually caused by the effects of mosquito-borne diseases, Aedes aegypti being the main carrier in the case of dengue, Zika, and chikungunya. The traditional methods to control insects using insecticides have become the cause of significant environmental issues, such as ecological imbalance, non-target toxicity, and development of resistance, and thus, sustainable alternatives are required. The olfactory receptors (ORs) are also important in the mediating host-seeking behaviour in mosquitoes as they detect volatile compounds of human origin. The paper used CRISPR-Cas9 gene editing to silence the genes of two olfactory receptors, Or4 and Or8, on the Ae. aegypti Liverpool strain. The guide RNAs were selected to target conserved domains and microinjection done on 300 embryos showed a survival rate of 47% and 68% editing efficiency was established by molecular analysis through frameshift mutations. In 2022-2023, behavioural assays revealed a 73% decrease in upwind flight response to human odor (p<0.001) of edited mosquitoes. Experiments in human landing catch have shown an 81% reduction in the rate of landing relative to wild-type controls, which implies a significant reduction in the degree of interaction between humans and vectors. Electrophysiological responses indicated that there was considerable attenuation of antennal responses to important human odorants including lactic acid, 1-octen-3-ol and ammonia. Confocal microscopy also demonstrated distressed glomerular targeting in olfactory sensory neurons to the antennal lobes. Notably, no meaningful impacts on the viability of mosquitoes and fecundity were detected, which implies behavioural change does not impair ecological fitness. Environ-wise, this is a non-lethal, eco-friendly approach to controlling vectors that helps to keep the ecosystem stable though the decrease of the disease transmission and the preservation of the mosquito population. The results give a basis in designing gene drive constructs that are used to suppress behaviour and not kill the population as it aims at achieving sustainable and eco-friendly control of vectors that do not cause harm to the environment and is less dependent on chemical intervention.
Artificial intelligence in bread making: Applications in quality control, formulation and sensory prediction Marimuthu Murugesan, Prakash Pandurangan, Anitha Murugesan, Halan Senthilkumar, Krishnamurthy K. Hegde, Sheela Thangaraj, Meivelu Moovendhan Food Chemistry X, 2026 Artificial intelligence (AI) is being applied by the bakery industry to substitute existing practices with data-informed strategies to enhance the accuracy of quality predictions, process optimization, and shelf-life prediction. This review discusses how AI, specifically machine learning (ML) algorithms such as artificial neural networks (ANNs), support vector machines (SVMs), random forests (RF) and deep neural networks (DNNs), can be applied in bread-making and product innovation. With AI models, there is the possibility of analyzing the interactions between formulation, processing and quality features in a complex manner to predict the loaf volume, crumb structure, staling and risk of spoilage. The optimization of formulations with the help of AI is also used to produce cost-effective, nutritionally fortified products without losing the sensory quality. Interest of AI in combination with improved methods like gas chromatography-olfactometry and texture profiling, E -nose/E-tongue systems improves the senses prediction prior to execution. Besides, the combination of AI and digital twins, kinetic models, and IoT systems enhance real-time analysis and operational performance, which can lead to sustainability, less food waste, and individualized and health-focused bakery items.
CRISPR-Cas9 Technology for Unlocking Immunity Against Infectious Pathogens M. Suriya, V. Aarthi, S. S. Meenambiga, P. Prakash, P. Vivek Crispr Cas9 Technology for Precision Medicine, 2026 The development of CRISPR-Cas9 has dramatically changed the field of genome engineering and its utilization in epigenome editing could have profound implications for precision medicine.This ability to precisely target epigenetic marks, including DNA methylation, histone modifications, and chromatin remodeling, allows for exciting therapeutic developments in cancer, neurodegenerative and autoimmune diseases. New developments such as CRISPRi, CRISPRa, and reversible CRISPR off/CRISPR on, allow for complex and multiplexed epigenetic reprogramming with improved specificity and safety. Nonetheless, there are some challenges to delivery off-target effects, stability of epigenetic modification, and moral lines, especially with inheritable epigenetic modification. The essential principles of CRISPR-Cas9-based epigenome editing, its therapeutic prospects for personalized medicine, and the consideration of technological, ethical, and policy aspects associated with epigenome editing are discussed
Collagen-based electrospun materials for drug delivery system Antony V. Samrot, D. Rajalakshmi, Harshaa Rajabalakumar, R. Sanjay Preeth, Madhumitha B.S., P. Prakash, Vinay Kumar Pandey, Gokul Shankar Sabesan Electrospun Biomaterials for Tissue Engineering and Drug Delivery Applications, 2025
Nanomaterials in the pretreatment of biomass Antony V. Samrot, D. Rajalakshmi, Harshaa Rajabalakumar, R. Sanjay Preeth, B.S. Madhumitha, P. Prakash, Vinay Kumar Pandey, Gokul Shankar Sabesan, Faidruz Azura Jam, Theebaa Anasamy Biomass Conversion Through Nanomaterials Green Sustainable Process for Chemical and Environmental Engineering and Science Gspcees, 2025
Correction to: Natural biopolymers as Scaffold Antony V. Samrot, M. Sathiya Sree, D. Rajalakshmi, L. Noel Richard Prakash, P. Prakash Drug Discovery and Design Using Natural Products, 2023
Natural biopolymers as scaffold Antony V. Samrot, M. Sathiya Sree, D. Rajalakshmi, L. Noel Richard Prakash, P. Prakash Drug Discovery and Design Using Natural Products, 2023
Phytomediated Adsorption of Acid Blue 113 using Ananas comosus Crown, Artocarpus heteropyllus Leaves and Spent Tea Leaves Indian Journal of Environmental Protection, 2022
A Review of Different Vaccines and Strategies to Combat COVID-19 Srinivasan Sabitha, Nagarajan Shobana, Pandurangan Prakash, Sathiyamoorthy Padmanaban, Mahendran Sathiyashree, Subramanian Saigeetha, Srikumar Chakravarthi, Saji Uthaman, In-Kyu Park, Antony V. Samrot Vaccines, 2022
Waste-Derived Cellulosic Fibers and Their Applications Antony V. Samrot, Khosa Tariro Ngaakudzwe, D. Rajalakshmi, P. Prakash, S. Suresh Kumar, M. Chandramohan, D. Alex Anand, J. Lilly Mercy, Yishak Simon, S. Saigeetha Advances in Materials Science and Engineering, 2022
Degradation of crude oil using the indigenous isolate Bacillus sp SEA18 Indian Journal of Biochemistry and Biophysics, 2020
Production and utilization of spions for in-vitro drug release and x-ray imaging Antony V. Samrot, Saipriya C, Durga Sruthi P., A. Jenifer Selvarani, Raji P., Prakash P, Paulraj Ponnaiah, Thirumurugan R, Sajeesh Pattammadath, Sajna Keeyari Purayil, Pazhayakath Thevarkattil Mohamed Javad, Iyappan P Journal of Pure and Applied Microbiology, 2020
Green synthesis and antibacterial activity studies of silver nanoparticles from the aqueous extracts of euphorbia hirta Department of Biotechnology, Sathyabama Institute of Science, Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India, Bennet Rohan D, Raji P, Department of Biotechnology, Sathyabama Institute of Science, Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India, Divya Kumar M, Department of Biotechnology, Sathyabama Institute of Science, Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India, Ratna Geethika Gandham, Department of Biotechnology, Sathyabama Institute of Science, Technology, Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India, Kripu Sharma V, Department of Biotechnology, Sathyabama Institute of Science, et al. Journal of Pure and Applied Microbiology, 2020
Production, characterization, and optimization of rhamnolipids produced by Pseudomonas aeruginosa by solid-state fermentation International Journal of Green Pharmacy, 2017
Bioprospecting endophytic fungus Colletotrichum sp. isolated from Artocarpus heterophyllus for anticancer activity Research Journal of Biotechnology, 2017
Micropropagation of Eclipta alba using humic acid as media component Plant Archives, 2015
Design of medium components for the enhanced production of mycoprotein by Fusarium venenatum using plackett burman model Research Journal of Pharmaceutical Biological and Chemical Sciences, 2015
Biological synthesis of gold nanoparticles using endophytic fungi Der Pharma Chemica, 2015
Evaluation of insecticidal activity of Fusarium venenatum metabolites againstsf-21 cell lines International Journal of Chemtech Research, 2015
Effect of vermiwash and humic acid on the germination and growth of Capsicum annum L and Abelmoschus esculentus Research Journal of Pharmaceutical Biological and Chemical Sciences, 2015
Evaluation of protein release rate from mycoprotein - Fusarium venenatum by cell disruption method International Journal of Pharmacy and Pharmaceutical Sciences, 2014
Screenig of bioactive compounds by Gc-Ms from fusarium venenatum International Journal of Pharmtech Research, 2014
Effect of humic acid on seed germination of Raphanus sativus L International Journal of Chemtech Research, 2014
Optimization of humic acid production using RSM-CCD, its characterization and applications on Vigna mungo International Journal of Chemtech Research, 2014
Optimization of growth promoters on Desmodium gangeticum (l) DC using RSM-CCD and its antioxidants activity International Journal of Pharmacy and Pharmaceutical Sciences, 2014
