Ahmed A Mahmoud

@mu.edu.eg

Ahmed A Mahmoud


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Scopus Publications

Scopus Publications

  • Syzygium cumini Fruit Extract and Quercetin Ameliorate Cadmium-Induced Ovarian Apoptosis in Rats Via miRNA- 204 - 5p-Mediated Bcl- 2 Upregulation and Bax/Caspase 9/Caspase 3 Downregulation
    Walaa I. E. Abdel-Badeea, Ahmed Abdel-Wahab, Ahmed A. Mahmoud, Abdel-Razik H. Abdel-Razik, Eman Z. Attia, et al.
    Biological Trace Element Research, 2025
    The correlation between exposure to environmental pollutants and adverse impacts on fertility has been documented. Cadmium (Cd) is one of the most toxic elements that impairs female reproductive capacity. Syzygium cumini is a plant that contains polyphenols with strong antioxidant properties. Our study aimed to investigate the possible protective effects of Syzygium cumini fruit extract (SCFE) and quercetin (QU) against Cd-induced ovarian toxicity and apoptosis in rats assisted by measuring oxidative stress markers, hormonal profile, intrinsic apoptotic pathway, miRNA-204-5p, and histopathology. For that, 45 adult female rats were divided randomly into five groups; control, cadmium chloride (CdCl2), CdCl2 + QU, CdCl2 + SCFE200, and CdCl2 + SCFE400, and the experiment lasted 35 days. Cadmium exposure induced irregular estrus cycle, and oxidative challenges, as evidenced by increased malondialdehyde and decreased antioxidant indicators that include superoxide dismutase, catalase and reduced glutathione, and hormonal imbalances, including reduced follicle-stimulating hormone, luteinizing hormone, anti-mullarian hormone, estrogen, and progesterone levels. Additionally, Cd triggered ovarian apoptosis through upregulating miRNA- 204 - 5p expression that in turn downregulated Bcl- 2 and upregulated the members of intrinsic mitochondrial apoptotic pathway including Bax and caspases 3, 9. The histopathological findings, morphometric and lesion scoring in ovarian, and uterine tissues confirmed the negative impacts of Cd. Interestingly, treatment with QU or SCFE, especially at the higher dose (400 mg/kg), significantly ameliorated these Cd-induced adverse effects, suggesting their potential as protective agents against ovarian toxicity.
  • 1,4,9,9-tetramethyloctahydro-4,7-(epoxymethano)azulen-5(1H)-one, a natural product as a potential inhibitor of COVID-19: Extraction, crystal structure, and virtual screening approach
    Youness El Bakri, Shaaban K. Mohamed, Kandasamy Saravanan, Sajjad Ahmad, Ahmed A. Mahmoud, et al.
    Journal of King Saud University Science, 2023
  • Physiological roles of red carrot methanolic extract and vitamin e to abrogate cadmium‐induced oxidative challenge and apoptosis in rat testes: Involvement of the bax/bcl‐2 ratio
    Ahmed Abdel-Wahab, Kamel M. A. Hassanin, Ahmed A. Mahmoud, Walaa I. E. Abdel-Badeea, Abdel-Razik H. Abdel-Razik, et al.
    Antioxidants, 2021
    The precise analysis of the contents of the red carrot is still ambiguous and its role in the maintenance of male fertility needs to be further reconnoitered. Hence, this study targets the physiological impacts of either red carrot methanolic extract (RCME) or vitamin E (Vit. E), co-administrated with cadmium chloride (CdCl2) on rat testes, specifically those concerned with apoptosis and oxidative challenge. Four groups of adult male rats (n = 12) are used; control, CdCl2, CdCl2 + Vit. E and CdCl2 + RCME. LC-MS analysis of RCME reveals the presence of 20 different phytochemical compounds. Our data clarify the deleterious effects of CdCl2 on testicular weights, semen quality, serum hormonal profile, oxidative markers and Bax/Bcl-2 ratio. Histopathological changes in testicular, prostatic and semen vesicle glandular tissues are also observed. Interestingly, our data clearly demonstrate that co-administration of either RCME or Vit. E with CdCl2 significantly succeeded in the modulation (p < 0.05) of all of these negative effects. The most striking is that they were potent enough to modulate the Bax/Bcl-2 ratio as well as having the ability to correct the impaired semen picture, oxidant status and hormonal profile. Thus, RCME and Vit. E could be used as effective prophylactic treatments to protect the male reproductive physiology against CdCl2 insult.
  • New Rare Ent-Clerodane Diterpene Peroxides from Egyptian Mountain Tea (Qourtom) and Its Chemosystem as Herbal Remedies and Phytonutrients Agents
    Taha A. Hussien, Ahmed A. Mahmoud, Naglaa S. Mohamed, Abdelaaty A. Shahat, Hesham R. El-Seedi, et al.
    Molecules, 2020
    Genus Stachys, the largest genera of the family Lamiaceae, and its species are frequently used as herbal teas due to their essential oils. Tubers of some Stachys species are also consumed as important nutrients for humans and animals due to their carbohydrate contents. Three new neo-clerodane diterpene peroxides, named stachaegyptin F-H (1, 2, and 4), together with two known compounds, stachysperoxide (3) and stachaegyptin A (5), were isolated from Stachys aegyptiaca aerial parts. Their structures were determined using a combination of spectroscopic techniques, including HR-FAB-MS and extensive 1D and 2D NMR (1H, 13C NMR, DEPT, 1H-1H COSY, HMQC, HMBC and NOESY) analyses. Additionally, a biosynthetic pathway for the isolated compounds (1–5) was discussed. The chemotaxonomic significance of the isolated diterpenoids of S. aegyptiaca in comparison to the previous reported ones from other Stachys species was also studied.
  • The anti-proliferative activity of anisosciadone: A new guaiane sesquiterpene from Anisosciadium lanatum
    Ahmed A. Mahmoud, Wael M. El-Sayed
    Anti Cancer Agents in Medicinal Chemistry, 2019
    Background: The increase in cancer rate and the development of resistant tumors require a continuous search for new anticancer agents. Aims: This study aimed to analyze and identify the chemical constituents of Anisosciadium lanatum, and to investigate the antiproliferative activity of the identified constituents against various human cell lines (HepG2, MCF7, HT29, A549, and PC3) along with the possible molecular mechanisms involved. Methods: The structure of the isolated compounds was determined by spectroscopic techniques including HRFABMS, GC-MS, IR, and 400 MHz 1D and 2D NMR analyses (1H, 13C NMR, DEPT, 1H-1H COSY, HMQC, HMBC and NOESY). The antiproliferative activity and IC50 value of the isolated compounds were measured and compared to doxorubicin. Results: A new guaiane sesquiterpene containing a rare epoxide structural element, 10β,11β−epoxy−1α,4β,5β,7αΗ- guaiane-9-one, anisosciadone (1), and stigmasterol (2) have been isolated from the plant. Anisosciadone (1) showed a significant antiproliferative activity against liver, colon, and lung cells only, while stigmasterol (2) had a significant activity against liver, colon, and breast cells. Both 1 and 2 caused no cytotoxicity to normal fibroblasts. Anisosciadone elevated the expression and activity of Caspase 3 as well as p53 expression without affecting Caspase 9 in HepG2 cells. It also caused ~ 50% downregulation in cdk1 expression. Conclusion: Taken together, anisosciadone was specific in action against cancer cells and induced apoptosis in liver cells. It also has a unique feature by elevating the expression and activity of Caspase 3 without affecting the initiator Caspase 9. Therefore, anisosciadone deserves more investigation as a targeted therapy for cancer.
  • Isolation, characterization and anticancer activity of seven compounds from the aerial parts of Conyza triloba
    International Journal of Pharmacognosy and Phytochemical Research, 2016
  • Antimicrobial and antioxidant activities of Artemisia abyssinica extracts and DNA degradation effects
    Magdy M. Youssef, Ahmed A. Mahmoud, Yasair S.S. Al-Faiyz
    Asian Journal of Biochemistry, 2015
  • The conyza triloba extracts with high chlorophyll content and free radical scavenging activity had anticancer activity in cell lines
    Wael M. El-Sayed, Warda A. Hussin, Ahmed A. Mahmoud, Mohamed A. AlFredan
    Biomed Research International, 2013
    The discovery of anticancer agents paradigm has been shifted to natural resources to overcome the toxicity of many synthetic agents at early clinical stages. In the present study, the antimutagenic, anticancer, phytochemistry, and free radical scavenging activities of five extracts ofConyza trilobawere investigated. Extracts II (water : methanol), III (methylene chloride), and IV (methylene chloride : methanol) had the highest chlorophyll content and the highest superoxide scavenging, and metal chelating activities comparable to that of trolox. They also showed DPPH•scavenging activities better than that ofα-tocopherol. Virtually all extracts exerted a strong (>40% reduction) antimutagenic activity against sodium azide and benzopyrene. Extracts II, III, and IV showed a remarkable growth inhibition profile with GI50of 0.07–0.87 μg for Hepa1c1c7 and H4IIE1, A549, HT29, and PC3 cell lines and totally abated the growth of all cell lines, except for the breast cells, at 0.3–7.0 μg. The present study found a strong correlation between the chlorophyll content ofConyzaextracts and their DDPH scavenging, metal chelating, and in vitro cytotoxic and cytostatic activities most probably through triggering apoptosis. This study could offer a platform for future studies and help selecting the vital features that identify the extract with potential anticancer activities.
  • A new epimeric sesquiterpene lactone from Achillea ligustica
    Records of Natural Products, 2012
  • Biological activity of a phloroglucinol glucoside derivative from Conyza aegyptiaca
    Ahmed A. Mahmoud, Shar S. Al-Shihry, Mohamed-Elamir F. Hegazy
    Zeitschrift Fur Naturforschung Section C Journal of Biosciences, 2009
    The phloroglucinol glucoside derivative [2,4-dihydroxy-6-(β-D-glucopyranosyloxy)phenyl]- butan-1-one (1), roseoside (2), and kaempferol-3-O-β-D-glucopyranoside (3) were isolated from the aerial parts of Conyza aegyptiaca (L.). To the best of our knowledge, this is the first isolation of compounds 1 - 3 from C. aegyptiaca. Their structures were determined by spectroscopic techniques including, IR, HR-EIMS, and extensive 500 MHz 1D- and 2D-NMR analyses (1H, 13C NMR, DEPT, 1H-1H COSY, HMQC and HMBC experiments). The antioxidant activity of 1, using the DPPH assay, was investigated; in addition, 1 was investigated against different types of cell lines, including Hep-G2, HCT-116, and RAW 264.7 for its cytotoxic effects. Also, this is the first report on the activity of 1.
  • Ketoisophorone transformation by Marchantia polymorpha and Nicotiana tabacum cultured cells
    Mohamed-Elamir F. Hegazy, Toshifumi Hirata, Ahmed Abdel-Lateff, Mohamed H. Abd El-Razek, Abou El-Hamd H. Mohamed, et al.
    Zeitschrift Fur Naturforschung Section C Journal of Biosciences, 2008
  • A new germacranolide-type sesquiterpene lactone from Tanacetum santolinoides
    Ahmed A. Mahmoud§, Mohammed A. Al-Omair, Munekazu Iinuma
    Natural Product Research, 2007
  • Lactate dehydrogenase isoenzyme pattern in the liver tissue of chemically-injured rats treated by combinations of diphenyl dimethyl bicarboxylate
    Laila Faddah, Nabil Abdel-Hamid, Yaser Abul-Naga, Sanaa Ibrahim, Ahmed Mahmoud
    Journal of Applied Biomedicine, 2007
  • Genus Chrysothamnus: A source of bioactive compounds
    Mohamed-Elamir F. Hegazy, Abou El-Hamd H. Mohamed, Mohamed H. Abd El-Razek, Fayza M. Hammouda, Nahed M. Hassan, et al.
    Natural Product Communications, 2007
  • α-Pinene-type monoterpenes and other constituents from Artemisia suksdorfii
    Ahmed A. Mahmoud, Ahmed A. Ahmed
    Phytochemistry, 2006
  • Jasomontanone, a novel bicyclic sesquiterpene from the leaves of Jasonia montana
    Ahmed A. Mahmoud
    Natural Product Communications, 2006
  • A new ionone glucoside and terpenoid constituents from Achillea Biebersteinii and their antifungal activity
    Ahmed A. Mahmoud, Shar S. Al-Shihry
    Natural Product Communications, 2006
  • A new heterocyclic glucoside from the fruits of Xanthium pungens
    Ahmed A. Mahmoud, Ahmed A. Ahmed, Shar S. Al-Shihry, Otmar Spring
    Natural Product Research, 2005
  • New monoterpenes from Mentha microphylla
    Ahmed A. Mahmoud
    Planta Medica, 2005
  • Diterpenoid quinones from Rosemary (Rosmarinus officinalis L.)
    Ahmed A. Mahmoud, Shar S. AL-Shihry, Byeng W. Son
    Phytochemistry, 2005
  • Nor-ent-kaurane diterpenes and hydroxylactones from Antennaria geyeri and Anaphalis margaritacea
    Ahmed A. Ahmed, Taha A. Hussein, Ahmed A. Mahmoud, Mohamed A. Farag, Paul W. Paré, et al.
    Phytochemistry, 2004
  • Terpenoid constituents of Aster subspicatus and A. ageratoides
    Pharmazie, 2002
  • Two highly oxygenated eudesmanes and 10 lignans from Achillea holosericea
    Ahmed A Ahmed, Ahmed A Mahmoud, Eptehal T Ali, Olga Tzakou, Maria Couladis, et al.
    Phytochemistry, 2002
  • Manoyl oxide α-arabinopyranoside and grindelic acid diterpenoids from Grindelia integrifolia
    Ahmed, Ahmed, Usama M. Ahmed, Ashraf A. El-Bassuony, Mohamed H. Abd El-Razk, et al.
    Journal of Natural Products, 2001
  • Erratum: Jasonol, a rare tricyclic eudesmane sesquiterpene and six other new sesquiterpenoids from Jasonia candicans (Tetrahedron (1998) 54 (8141) PII: S0040402098004530)
    Ahmed A Ahmed, Ahmed A Mahmoud
    Tetrahedron, 2000