Ahmed A Mahmoud
@mu.edu.eg
Scopus Publications
Scopus Publications
Youness El Bakri, Shaaban K. Mohamed, Kandasamy Saravanan, Sajjad Ahmad, Ahmed A. Mahmoud, Shaban A.A. Abdel-Raheem, Wael M. El-Sayed, Joel T. Mague, and Souraya Goumri Said
Elsevier BV
Ahmed Abdel-Wahab, Kamel M. A. Hassanin, Ahmed A. Mahmoud, Walaa I. E. Abdel-Badeea, Abdel-Razik H. Abdel-Razik, Eman Zekry Attia, Usama Ramadan Abdelmohsen, Rabie L. Abdel Aziz, Agnieszka Najda, Ibtesam S. Alanazi,et al.
MDPI AG
The precise analysis of the contents of the red carrot is still ambiguous and its role in the maintenance of male fertility needs to be further reconnoitered. Hence, this study targets the physiological impacts of either red carrot methanolic extract (RCME) or vitamin E (Vit. E), co-administrated with cadmium chloride (CdCl2) on rat testes, specifically those concerned with apoptosis and oxidative challenge. Four groups of adult male rats (n = 12) are used; control, CdCl2, CdCl2 + Vit. E and CdCl2 + RCME. LC-MS analysis of RCME reveals the presence of 20 different phytochemical compounds. Our data clarify the deleterious effects of CdCl2 on testicular weights, semen quality, serum hormonal profile, oxidative markers and Bax/Bcl-2 ratio. Histopathological changes in testicular, prostatic and semen vesicle glandular tissues are also observed. Interestingly, our data clearly demonstrate that co-administration of either RCME or Vit. E with CdCl2 significantly succeeded in the modulation (p < 0.05) of all of these negative effects. The most striking is that they were potent enough to modulate the Bax/Bcl-2 ratio as well as having the ability to correct the impaired semen picture, oxidant status and hormonal profile. Thus, RCME and Vit. E could be used as effective prophylactic treatments to protect the male reproductive physiology against CdCl2 insult.
Taha A. Hussien, Ahmed A. Mahmoud, Naglaa S. Mohamed, Abdelaaty A. Shahat, Hesham R. El-Seedi, and Mohamed-Elamir F. Hegazy
MDPI AG
Genus Stachys, the largest genera of the family Lamiaceae, and its species are frequently used as herbal teas due to their essential oils. Tubers of some Stachys species are also consumed as important nutrients for humans and animals due to their carbohydrate contents. Three new neo-clerodane diterpene peroxides, named stachaegyptin F-H (1, 2, and 4), together with two known compounds, stachysperoxide (3) and stachaegyptin A (5), were isolated from Stachys aegyptiaca aerial parts. Their structures were determined using a combination of spectroscopic techniques, including HR-FAB-MS and extensive 1D and 2D NMR (1H, 13C NMR, DEPT, 1H-1H COSY, HMQC, HMBC and NOESY) analyses. Additionally, a biosynthetic pathway for the isolated compounds (1–5) was discussed. The chemotaxonomic significance of the isolated diterpenoids of S. aegyptiaca in comparison to the previous reported ones from other Stachys species was also studied.
Ahmed A. Mahmoud and Wael M. El-Sayed
Bentham Science Publishers Ltd.
Background: The increase in cancer rate and the development of resistant tumors require a continuous search for new anticancer agents. Aims: This study aimed to analyze and identify the chemical constituents of Anisosciadium lanatum, and to investigate the antiproliferative activity of the identified constituents against various human cell lines (HepG2, MCF7, HT29, A549, and PC3) along with the possible molecular mechanisms involved. Methods: The structure of the isolated compounds was determined by spectroscopic techniques including HRFABMS, GC-MS, IR, and 400 MHz 1D and 2D NMR analyses (1H, 13C NMR, DEPT, 1H-1H COSY, HMQC, HMBC and NOESY). The antiproliferative activity and IC50 value of the isolated compounds were measured and compared to doxorubicin. Results: A new guaiane sesquiterpene containing a rare epoxide structural element, 10β,11β−epoxy−1α,4β,5β,7αΗ- guaiane-9-one, anisosciadone (1), and stigmasterol (2) have been isolated from the plant. Anisosciadone (1) showed a significant antiproliferative activity against liver, colon, and lung cells only, while stigmasterol (2) had a significant activity against liver, colon, and breast cells. Both 1 and 2 caused no cytotoxicity to normal fibroblasts. Anisosciadone elevated the expression and activity of Caspase 3 as well as p53 expression without affecting Caspase 9 in HepG2 cells. It also caused ~ 50% downregulation in cdk1 expression. Conclusion: Taken together, anisosciadone was specific in action against cancer cells and induced apoptosis in liver cells. It also has a unique feature by elevating the expression and activity of Caspase 3 without affecting the initiator Caspase 9. Therefore, anisosciadone deserves more investigation as a targeted therapy for cancer.
Magdy M. Youssef, Ahmed A. Mahmoud, and Yasair S.S. Al-Faiyz
Science Alert
Wael M. El-Sayed, Warda A. Hussin, Ahmed A. Mahmoud, and Mohamed A. AlFredan
Hindawi Limited
The discovery of anticancer agents paradigm has been shifted to natural resources to overcome the toxicity of many synthetic agents at early clinical stages. In the present study, the antimutagenic, anticancer, phytochemistry, and free radical scavenging activities of five extracts ofConyza trilobawere investigated. Extracts II (water : methanol), III (methylene chloride), and IV (methylene chloride : methanol) had the highest chlorophyll content and the highest superoxide scavenging, and metal chelating activities comparable to that of trolox. They also showed DPPH•scavenging activities better than that ofα-tocopherol. Virtually all extracts exerted a strong (>40% reduction) antimutagenic activity against sodium azide and benzopyrene. Extracts II, III, and IV showed a remarkable growth inhibition profile with GI50of 0.07–0.87 μg for Hepa1c1c7 and H4IIE1, A549, HT29, and PC3 cell lines and totally abated the growth of all cell lines, except for the breast cells, at 0.3–7.0 μg. The present study found a strong correlation between the chlorophyll content ofConyzaextracts and their DDPH scavenging, metal chelating, and in vitro cytotoxic and cytostatic activities most probably through triggering apoptosis. This study could offer a platform for future studies and help selecting the vital features that identify the extract with potential anticancer activities.
Ahmed A. Mahmoud, Shar S. Al-Shihry, and Mohamed-Elamir F. Hegazy
Walter de Gruyter GmbH
The phloroglucinol glucoside derivative [2,4-dihydroxy-6-(β-D-glucopyranosyloxy)phenyl]- butan-1-one (1), roseoside (2), and kaempferol-3-O-β-D-glucopyranoside (3) were isolated from the aerial parts of Conyza aegyptiaca (L.). To the best of our knowledge, this is the first isolation of compounds 1 - 3 from C. aegyptiaca. Their structures were determined by spectroscopic techniques including, IR, HR-EIMS, and extensive 500 MHz 1D- and 2D-NMR analyses (1H, 13C NMR, DEPT, 1H-1H COSY, HMQC and HMBC experiments). The antioxidant activity of 1, using the DPPH assay, was investigated; in addition, 1 was investigated against different types of cell lines, including Hep-G2, HCT-116, and RAW 264.7 for its cytotoxic effects. Also, this is the first report on the activity of 1.
Mohamed-Elamir F. Hegazy, Toshifumi Hirata, Ahmed Abdel-Lateff, Mohamed H. Abd El-Razek, Abou El-Hamd H. Mohamed, Nahed M. Hassan, Paul W. Paré, and Ahmed A. Mahmoud
Walter de Gruyter GmbH
Stereospecific olefin (C=C) and carbonyl (C=O) reduction of the readily available prochiral compound ketoisophorone (2,2,6-trimethyl-2-cyclohexene-1,4-dione) (1) by Marchantia polymorpha and Nicotiana tabacum cell suspension cultures produce the chiral products (6R)-levodione (2), (4R,5S)-4-hydroxy-3,3,5-trimethylcyclohexanone (3), and (4R,6R)-actinol (4) as well as the minor components (4R)-hydroxyisophorone (5) and (4S)-phorenol (6).
Ahmed A. Mahmoud§, Mohammed A. Al-Omair, and Munekazu Iinuma
Informa UK Limited
A new germacranolide-type sesquiterpene lactone, 1α-hydroxy-3-oxo-7α,11βH-germacra-4Z,9Z-dien-12,6α-olide (1), was isolated from the CH2Cl2--MeOH extract of the aerial parts of Tanacetum santolinoides. Its structure was determined by spectroscopic techniques including, IR, high-resolution-EIMS, and extensive 400 MHz one- and two-dimensional NMR-analysis (1H, 13C-NMR, DEPT, 1H--1H COSY, HMQC, HMBC, and NOE experiments).
Mohamed-Elamir F. Hegazy, Abou El-Hamd H. Mohamed, Mohamed H. Abd El-Razek, Fayza M. Hammouda, Nahed M. Hassan, Usama A. Mahalel, Ali M. El-Halawany, Ahmed A. Mahmoud, Joe Karchesy, Toshifumi Hirata,et al.
SAGE Publications
This review is written to take into account the rapid development in the taxonomy and phytochemistry of Chrysothamnus species. The article reviews the usage and biological activities of Chrysothamnus compounds, as well as recent progress regarding the use of this genus in clinical research.
Laila Faddah, Nabil Abdel-Hamid, Yaser Abul-Naga, Sanaa Ibrahim, and Ahmed Mahmoud
University of South Bohemia in Ceske Budejovice
The purpose of our study is to evaluate the protective effect of diphenyl dimethyl bicarboxylate (DDB) in combination with some antioxidants, namely vitamin C (V.C), vitamin E (V.E), and selenium (Se), in liver damage induced by carbon tetrachloride (0.2 ml/kg body weight). This was done by monitoring the liver total and fractional lactate dehydrogenase (LDH) activities. The results revealed a significant increase in the activity of liver total LDH activity in CCl4 intoxicated rats with a significant increase in both LDH3 and 4 and a significant decrease in LDH5. LDH2 disappeared after CCl4 treatment and neither DDB nor its combinations could restore this permanent change. DDB alone significantly decreased the CCl4-raised total LDH and LDH4, but still far from the control and failed to correct LDH3 and 5 variations. A combination of DDB and V.C, V.E and Se showed the best corrective potential in both total LDH and LDH3 activities, without correcting the increased LDH4, nor the decreased LDH5 isoenzyme. Although this combination was previously reported to correct liver function disturbances, it seems that CCl4 and consequently hepatitis C may induce some irreversible, non-curable changes by DDB or even by additional antioxidants. Its clinical usefulness seems to be through different metabolic pathways, not including correction of LDH disturbances, which necessitates additional investigation for other adjunct medicines for treating liver fibrosis in clinical practice.
Ahmed A. Mahmoud and Ahmed A. Ahmed
Elsevier BV
Ahmed A. Mahmoud and Shar S. Al-Shihry
SAGE Publications
A new ionone glucoside, (-)(4R*, 5R*)-5-(β-D-glucopyranosyloxymethyl)-4-hydroxy-3,5-dimethyl-4-((E)-3-oxobut-1-enyl)cyclohex-2-enone (biebersteiniside) (1), in addition to four known compounds, 6-epiroseoside (2), ascaridole (3), strictic acid (4) and centipedic acid (5) have been isolated from the aerial parts of Achillea biebersteinii Afan. The structures were determined from extensive 500 MHz NMR 1D (1H and 13C NMR) and 2D (1H-1H COSY, HMQC, HMBC and NOESY) spectroscopic data. Compounds 2–5 are reported for the first time from this plant. In addition, compounds 1a and 3–5 showed antifungal activity.
Ahmed A. Mahmoud
SAGE Publications
A novel rearranged eudesmane sesquiterpene with a new 5/7 membered bicyclic-ring-system, jasomontanone, was isolated from Jasonia montana. Its structure was determined as (3a R*, 6 R*, 8a R)-3a-(hydroxymethyl)-6-(2-hydroxy-propan-2-yl)-8a-methyl-octahydrazulen-4(5 H)-one by analysis of spectroscopic data (IR, HR-MS, 1H and 13C NMR), including 2D NMR (1H-1H COSY, HMQC, HMBC and NOESY), chemical transformation, and biogenetic consideration.
Ahmed A. Mahmoud, Ahmed A. Ahmed, Shar S. Al-Shihry, and Otmar Spring
Informa UK Limited
A new thiazinedione glucoside, 7-(β-D-glucopyranosyloxymethyl)-8,8-dimethyl-4,8-dihydrobenzo[1,4]thiazine-3,5-dione (1) and named xanthiside, was isolated from the methanol extract of the fruits of Xanthium pungens. Its structure was determined by spectroscopic techniques including, IR, CIMS, high-resolution-EIMS, and extensive 400 MHz one- and two-dimensional NMR-analysis (1H, 13C-NMR, DEPT, 1H-1H COSY, HMQC, COLOC, and NOE experiments).
Ahmed A. Mahmoud
Georg Thieme Verlag KG
Four new monoterpenes, (1 R*,2 S*)-1,2-dihydroxy- p-menth-4(8)-en-3-one ( 1), (4 S*)-4-hydroxy- p-mentha-1,8-dien-3-one ( 2), (4 S*)-4-methoxy- p-mentha-1,8-dien-3-one ( 3), and (3 R*,4 R*,6 S*)-3-acetoxy-6-hydroxy- p-mentha-1,8-diene ( 4) together with four known compounds have been isolated from the aerial parts of Mentha microphylla. The structures were determined using spectroscopic methods particularly high resolution (1)H-, (13)C-NMR as well as 2D (1)H- (1)H COSY, HMQC and HMBC analysis.
Ahmed A. Mahmoud, Shar S. AL-Shihry, and Byeng W. Son
Elsevier BV
Ahmed A. Ahmed, Taha A. Hussein, Ahmed A. Mahmoud, Mohamed A. Farag, Paul W. Paré, Małgorzata Wojcińska, Joe Karchesy, and Tom J. Mabry
Elsevier BV
Ahmed A Ahmed, Ahmed A Mahmoud, Eptehal T Ali, Olga Tzakou, Maria Couladis, Tom J Mabry, Tamás Gáti, and Gábor Tóth
Elsevier BV
Ahmed, Ahmed, Usama M. Ahmed, Ashraf A. El-Bassuony, Mohamed H. Abd El-Razk, Paul W. Pare, and Joe Karchesy
American Chemical Society (ACS)
Two new manoyl oxide-alpha-arabinopyranoside diterpenoids, 15-hydroxy-13-epi-manoyl oxide-14-O-alpha-L-arabinopyranoside (tarapacol-14-O-alpha-L-arabinopyranoside) (1) and 15-acetoxy-13-epi-manoyl oxide-14-O-alpha-L-arabinopyranoside (tarapacol-15-acetate-14-O-alpha-L-arabinopyranoside) (2), as well as a new grindelic acid derivative, 19-hydroxygrindelic acid (3), together with five known diterpenoids (tarapacol, tarapacanol A, grindelic acid, methyl grindeloate, 3beta-hydroxygrindelic acid, 4) were isolated from the aerial parts of Grindelia integrifolia. The structures of 1-3 were elucidated by spectral data analysis.
Ahmed A Ahmed and Ahmed A Mahmoud
Elsevier BV
Ahmed A. Mahmoud, Ahmed, Toshiyuko Tanaka, and Munekazu Iinuma
American Chemical Society (ACS)
Two new norditerpenoid acids of the labdane-type (norgrindelic acids), 4,5-dehydro-6-oxo-18-norgrindelic acid (1) and 4beta-hydroxy-6-oxo-19-norgrindelic acid (2), as well as a new grindelic acid derivative, 18-hydroxy-6-oxogrindelic acid (3), were isolated from the aerial parts of Grindelia nana. In addition, the known compounds, 6-oxogrindelic acid, grindelic acid, methyl grindeloate, 7alpha,8alpha-epoxygrindelic acid, and 4alpha-carboxygrindelic acid were also isolated. The structures of the new compounds were characterized on the basis of spectroscopic analysis.