Pharmacy, Drug technology, Biopharmaceutical researches
11
Scopus Publications
55
Scholar Citations
4
Scholar h-index
1
Scholar i10-index
Scopus Publications
Development and Optimization of Polymer-Based Dissolving Microneedles Fabricated by Mold Casting Method Liubov Bodnar, Tetiana Kovalova, Volodymyr Yakovenko, Oleh Koshovyi, Kaloyan D. Georgiev, Iliya Zhelev Slavov, Liliia Vyshnevska Polymers, 2026 Microneedle systems represent a promising minimally invasive approach for transdermal drug delivery; however, their performance strongly depends on the composition and mechanical properties of the polymer matrix. The aim of this study was to select an optimal polymer composition for the fabrication of dissolving microneedle arrays produced by the mold casting method. The study focused on evaluating mechanical strength, dissolution behavior, and penetration efficiency of different polymer systems. Microneedle matrices were fabricated using polyvinylpyrrolidone (PVP K-30), methylcellulose, sodium alginate, and hyaluronic acid at various concentrations, alone and in combination. No active pharmaceutical ingredient (API) was incorporated; the study was performed using blank polymeric systems intended for subsequent drug loading. The microneedles were manufactured using 3D-printed and silicone molds. Their performance was evaluated by in vitro dissolution testing, pH measurement, penetration studies in gelatin gel and Parafilm M models, and mechanical compression testing. Monopolymer systems demonstrated either rapid dissolution with insufficient mechanical strength or improved strength at the expense of prolonged dissolution time. Combined polymer formulations showed superior structural uniformity and balanced performance. In particular, the system containing 5% PVP K-30 and 10% sodium alginate demonstrated the best overall characteristics, achieving high penetration efficiency (up to 96%), uniform dissolution (78%), and appropriate dissolution time (8.5 ± 0.5 min). Addition of hyaluronic acid further improved structural uniformity and handling properties. The results indicate that composite polymer matrices provide an optimal balance between mechanical stability, penetration ability, and dissolution rate. The formulation consisting of 5% PVP K-30 and 10% sodium alginate was identified as the most promising base for further development of drug-loaded dissolving microneedle systems.
ANALYSIS OF THE CURRENT STATE OF DEVELOPMENT OF MICRO-NEEDLE SYSTEMS FOR TRANSDERMAL DRUG DELIVERY (A SCOPING REVIEW) Liubov Bodnar, Tetiana Kovalova, Natalia Sydora, Oleksandr Shmalko, Ihor Berdey, Liliia Vyshnevska Sciencerise Pharmaceutical Science, 2026 The aim is to examine the current state of development of microneedle systems for transdermal drug delivery.Materials and methods. Analysis, systematization, and generalization of data from scientific literature sources on the development of microneedle systems, research on their effectiveness, and prospects for use in pharmacy. A modified Arskey O'Malley methodology was used, refined by a research group led by H.M. Daudt. A total of 480 publications from the last ten years were analysed.Results. The results of the analysis show that most of the experimental studies focus on the development of microneedle systems for transdermal delivery of vaccines, insulin, and analgesics. In contrast, studies devoted to the creation of drugs with a different direction of action or drugs for the gradual release of active substances using microneedles occupy a smaller segment of the total number.Conclusions. It has been established that most scientists choose to develop microneedle-based drugs for systemic use. The main areas of publication are reviews of literature sources, the development of new microneedle systems, etc. The results obtained indicate the potential and relevance of conducting research on the development of microneedle systems
CURRENT STATE OF SCIENTIFIC RESEARCH ON PHARMACOLOGICAL CORRECTION OF MAMMARY GLAND PATHOLOGIES (A SCOPING REVIEW) Polina Palyvoda, Svitlana Zuikina, Volodymyr Yakovenko, Liubov Bodnar, Oleksandr Shmalko Sciencerise Pharmaceutical Science, 2025 The aim of the study is to conduct a bibliosemantic analysis of scientific literature on pharmacological correction of breast pathologies and to determine the proportion of studies on pharmacological correction of mastopathy.Materials and methods. In the context of our study, the analysis of data on the current state of research on the pharmacological correction of mammary gland pathologies and the identification of the proportion of studies focusing on the pharmacological correction of mastopathy was carried out using the enhanced Arksey & O’Malley methodology proposed by a group of researchers led by H.M. Daudt. A total of 540 publications (from the last five years) were reviewed.Results. The results of the study showed that the vast majority of publications are devoted to the pharmacological correction of breast cancer (78%). Studies on the pharmacological correction of mastopathy make up a significantly smaller share (22%), which confirms their relevance, particularly regarding the development of original drugs. Among these, herbal-based medicinal products are most commonly used as part of combination therapy (59%).Conclusions. Studying the current state of scientific research on a given topic is an integral part of planning and developing a strategy for one's own research. A detailed analysis of the available literature makes it possible to identify unresolved issues that remain unaddressed by researchers from different countries, and to outline relevant niches for further scientific investigation.A bibliosemantic analysis of scientific literature on pharmacological correction of breast pathologies was conducted, and the relevance of conducting research on the development of herbal remedies for the pharmacological correction of mastopathy was established.
DEVELOPMENT OF THE COMPOSITION AND TECHNOLOGY OF PREPARATION OF A SUSPENSION WITH ADAPALENE FOR THE TREATMENT OF ACNE TAKING INTO ACCOUNT THE BIOPHARMACEUTICAL FACTORS Marina Buryak, Liubov Bodnar, Natalya Shtuchna, Svitlana Oliinyk, Kateryna Semchenko, Liliia Vyshnevska Sciencerise Pharmaceutical Science, 2025 The aim of the research is the development of the suspension with adapalene for use in the symptomatic block of complex acne therapy composition and technology of preparation. Materials and methods. The objects of the study were suspension samples, which included active pharmaceutical ingredients (adapalene, hyaluronic acid, liquid aloe extract and zinc oxide), and purified water. Results. Hyaluronic acid was determined as the optimal stabilizer and its concentration was selected using the resuspension method, at which the suspension is a stable system. It was established that the samples, which include adapalene, zinc oxide, liquid aloe extract, hyaluronic acid and purified water, have satisfactory physical and chemical properties. Conducted rheological studies showed that due to the potentiation of associative thickening, optimal rheological indicators of the suspension, which affect both the extrusion and consumption properties of the developed suspension, were obtained. The quantitative content of adapalene in the developed suspension was confirmed by the method of alkalimetry. The completeness and speed of release of adapalene from the suspension was confirmed by the method of dialysis through a semipermeable membrane. Conclusions. An extemporaneous suspension, based on adapalene, zinc oxide and liquid aloe extract, with the addition of a stabilizer – hyaluronic acid, was developed. The proposed suspension has satisfying consumer, physical and chemical properties, and meets the quality requirements established for suspensions
DEVELOPMENT OF THE COMPOSITION OFA DERMATOLOGICAL PRODUCT IN THE FORM OFA CREAM WITH EXTRACTS OF THE AERIAL PART OF LESPEDEZA BICOLOR Kate Kiselyova, Liliia Vyshnevska, Tetiana Yudkevych, Liubov Bodnar, Mariia Skybitska, Liudas Ivanauskas, Olha Mykhailenko, Oleksandr Kukhtenko, Victoriya Georgiyants Sciencerise Pharmaceutical Science, 2025 The aim of the work: Justification of the composition of emulsion cream with extracts of Lespedeza bicolor.Materials and methods: In the development of the emulsion base of the cream, corn oil (Ukraine), propylene glycol (Germany), purified water, emulsifiers: xyliance (France), prolipid 141 (USA) and lamecrem (France) were used. The composition of the emulsion base included oil and liquid alcohol extract of the above-ground part of Lespedeza bicolor, which were obtained from the above-ground part of Lespedeza bicolor, harvested in the Botanical Garden of the Ivan Franko National University of Lviv (Lviv, Ukraine) in the flowering phase. The studies used physicochemical (pH value, identification and quantitative content of BAS), pharmacotechnological (thermo- and colloidal stability, structural-mechanical properties and disperse analysis) research methods. The anti-inflammatory activity of the experimental samples was studied on a burn wound model in outbred sexually mature male rats. The following medicines were used as comparison drugs: Panthenol ointment, “Hemofarm”, AD, Serbia, series 138CLA and Calendula ointment, LLC “Pavlova Pharmacy”, Ukraine, series 23.0823.Results. Experimental studies of the organoleptic, physicochemical and structural-mechanical properties of samples of emulsion bases showed their dependence on the concentration of the oil phase, the composition and concentration of complex emulsifiers xyliance, prolipid 141 and lamecrem. It was established that the emulsion base of the cream, which contains 15 % corn oil, 7 % of the complex emulsifier xyliance, 5 % propylene glycol and water purified to 100, has satisfactory structural-mechanical properties, the necessary dispersion and homogeneous distribution of particles of the oil phase in the aqueous dispersion medium, withstands the test for thermal and colloidal stability and can be used to develop a dermatological cream with Lespedeza extracts. It was shown that the introduction of extracts into the composition of the developed base does not affect the stability, structural-mechanical properties of the base and the content of BAS. Studies of the anti-inflammatory activity of a cream with a complex of biologically active compounds of the oily and alcoholic extracts of the terrestrial part of Lespedeza bicolor on a burn wound model showed a reduction in signs of inflammation without signs of the joining of an infectious process in laboratory animals with wound healing on the 26th day of the study.Conclusions. The composition of the cream with oil and alcohol extract of Lespedeza, which, due to the BAS complex, has a wider spectrum of pharmacological activity, has been experimentally substantiated. The composition of the emulsion base of the cream with corn oil, xyliance emulsifier, propylene glycol and purified water has been developed. It has been shown that the introduction of extracts into the composition of the developed base does not affect its pharmacotechnological properties, and the quantitative content of biologically active compounds in the cream corresponds to their content in the extracts in terms of the concentration of extracts in SDF, which is a confirmation of the compatibility of BAS with excipients. It has been established that the cream containing the BAS complex of Lespedeza bicolor in terms of anti-inflammatory activity is at the level of the comparison preparations Panthenol ointment and Calendula ointment
RESEARCH ON THE DEVELOPMENT OF SOLID SOAP COMPOSITION FOR THE PREVENTION AND TREATMENT OF PSORIASIS RELAPSE Ivan Honcharov, Liliia Vyshnevska, Liubov Bodnar Sciencerise Pharmaceutical Science, 2024 The aim of the research is to develop the composition of solid soaps for the treatment and prevention of exacerbation of psoriasis using a classic recipe based on vegetable oils.Materials and methods. The studied samples of solid soaps were made based on vegetable oils of domestic and foreign production using an aqueous solution of sodium hydroxide for their saponification. Citric acid (China, p. K707784), salicylic acid (China, p. Y1504007) and lactobionic acid (Poland, p. 20161118) were used as active pharmaceutical ingredients. Organoleptic (appearance, shape, color, smell) and physico-chemical (mass fraction of fatty acids, initial volume of foam, mass fraction of soda products) studies were carried out following DSTU 4537:2006 Solid toilet soap. General technical conditions. Indicators were chosen as standards for soap of the special - therapeutic brand.Results. Samples containing a combination of coconut, avocado, castor, jojoba and palm oils, as well as a combination of castor, jojoba, macadamia, mango and avocado oils were found to have insufficient foaming capacity. Most samples are not firm enough, especially those containing rosehip oil. The composition of solid soap based on palm kernel oil, coconut oil, avocado oil, castor oil, almond oil, and olive oil, among others, showed the best organoleptic properties. All the studied samples were within the normal range according to the parameters of the mass fraction of soda products and the mass fraction of fatty acids. At the same time, introducing active substances into the composition did not lead to a deterioration of the developed solid soap's normative or controlled organoleptic and physicochemical properties.Conclusions. The research conducted made it possible to obtain the optimal composition of solid soap, which can be used to prevent psoriasis exacerbation due to the introduction of pomegranate seed oil. Based on this, solid soaps were created for the treatment of psoriasis in the period of exacerbation, one of which includes lactobionic acid and the other – a combination of citric and salicylic acids
DEVELOPMENT OF AN EMULSION COMPOSITION WITH FENNEL AND CARAWAY ESSENTIAL OILS FOR USE IN THE COMBINED THERAPY OF ULCERATIVE COLITIS Oleksandr Shmalko, Tetiana Kovalova, Liubov Bodnar, Volodymyr Kovalov, Volodymyr Yakovenko, Liliia Vyshnevska Sciencerise Pharmaceutical Science, 2024 The aim of the study is to develop the composition of an emulsion containing essential oils of Fennel and Caraway seeds for use in the symptomatic complex therapy of ulcerative colitis with the aim of eliminating functional intestinal disorders.Materials and methods. The objects of the study were samples of emulsions containing active pharmaceutical ingredients (essential oils of Fennel and Caraway), purified water, oils (refined sunflower oil, refined olive oil, refined sesame oil), emulsifiers (polyethylene glycol 40 hydrogenated castor oil, polysorbate 80, polyethylene glycol 100 stearate, acacia gum, guar gum, xanthan gum, soya lecithin), viscosity regulator - apple pectin and flavouring agents (food additives with cherry and tarragon flavour).Organoleptic properties, stability, rheological parameters, pH, particle size determination by microscopy, and a taste test were carried out with model emulsion samples. Research to establish the optimal technological parameters was carried out in parallel.Results. The main parameters of the technological process have been established, which allow to obtain an emulsion with evenly distributed particles: 15 minutes at maximum speed. The concentration of emulsifiers at which the emulsions are stable was selected. It was found that the samples containing polyethylene glycol 100 stearate, gums, and soy lecithin have satisfactory organoleptic properties.The sample with soy lecithin emulsifier differs from the others in its ability to form microemulsions, but it has low viscosity. To improve the rheological properties, apple pectin was added to the emulsion.The taste test showed that among vegetable oils, refined sesame oil has a more neutral taste, and the flavouring additive "Tarkhun" balances the taste better.The release of active pharmaceutical ingredients (APIs) from the emulsion base was confirmed by thin-layer chromatography.Conclusions. A microemulsion with essential oils based on refined sesame oil, soy lecithin, with the addition of viscosity and flavour correctors was developed. The obtained emulsion has satisfactory organoleptic properties and conforms to the requirements for emulsion quality indicators
CONDUCTING BIOLOGICAL TESTS IN THE DEVELOPMENT OF SELF-EMULSIFYING DRUG DELIVERY SYSTEMS WITH SIMVASTATIN Liubov Bodnar, Dmytro Lytkin, Nataliia Polovko Sciencerise Pharmaceutical Science, 2023 The aim of the study was to compare the hypolipidemic activity of the developed self-emulsifying drug delivery systems with simvastatin with reference samples of the substance and the finished drug product of industrial production. Materials and methods. The substance of simvastatin (India, s. DK40-2005021, 99.09 %, introduced into the composition of self-emulsifying compositions based on castor oil (Ukraine), polyethylene glycol 40 hydrogenated castor oil (India), Tween 80 (Ukraine), glycerol monostearate (Gustav Heess GmbH, Germany) or polyethylene glycol 100 stearate (ERCA, Italy). Reference samples were Simvastatin-Sandoz (Salutas Pharma, Germany, series LX5161) and simvastatin in pure form. The experimental animals were Syrian hamsters aged 2 months. Hyperlipidemia was modeled by means of an alimentary load. To assess the state of lipid metabolism in animals, the content of triacylglycerols, total cholesterol, low-density lipoprotein and high-density lipoprotein in the blood serum was determined by colorimetric enzymatic methods using the appropriate standard reagent kits "Triacylglycerols F" HP022.02, "Cholesterol F" HP026.02, "Cholesterol-LDL F" HP026.05 and "Cholesterol-HDL F" HP026.04 (LLC SPE "Filicit-Diagnostics", Ukraine) on a semi-automatic biochemical analyzer MapLab Plus (BSI, Italy). Results. The reference samples had similar dose-dependent efficacy parameters. At the same time, the test samples, also having similar dose-dependent effects, in absolute terms at the maximum concentration reduced the amount of low-density lipoprotein and total cholesterol more effectively than the reference samples. When using the test samples in their average concentration, the level of triglycerols was significantly reduced, which is rather a concomitant effect of simvastatin. Conclusions. The improvement of the overall efficacy of simvastatin when it is introduced into self-emulsifying drug delivery systems has been proved, which is associated with the modification of pharmacokinetic parameters by improving the solubility of the substance in the aqueous environment of the gastrointestinal tract
BIOPHARMACEUTICAL JUSTIFICATION OF THE CREATION OF SELF-EMULSIFYING DRUG DELIVERY SYSTEMS WITH SIMVASTATIN Liubov Bodnar, Nataliia Polovko, Nataliia Bevz, Volodymyr Hrudko, Olesia Perepelytsia Sciencerise Pharmaceutical Science, 2023 The aim of the research – to conduct biopharmaceutical tests of capsules with a self-emulsifying delivery system of simvastatin to confirm the effectiveness and feasibility of introducing into the composition of self-emulsifying drug delivery systems active pharmaceutical ingredients that are difficult to dissolve in the gastric juice environment.
 Material and methods. Substances, excipients, reagents and materials used during research were simvastatin (India, p. DK40-2005021, 99.09 %), castor oil (Ukraine), polyethylene glycol 40 hydrogenated castor oil (India), Tween 80 (Ukraine), glycerol monostearate (Gustav Heess GmbH, Germany), polyethylene glycol 100 stearate (ERCA, Italy), hard gelatin capsules No. 3 white (China), 0.1 M hydrochloric acid solution (made from concentrated hydrochloric acid), ethanol 96 % (Ukraine), filter paper 90 mm white tape (Ukraine). The reference drug is "Simvastatin-Sandoz" (Salyutas Pharma, Germany, series LX5161).
 An Evolution 60S spectrophotometer (USA) was used to carry out studies by absorption spectrophotometry.
 Results. Preliminary study of the absorption spectra of absorption of the substance, bases, gelatin capsules, developed self-emulsifying systems and the reference drug made it possible to predict and optimize the conduct of biopharmaceutical research. The study of the release of simvastatin from the developed delivery systems and the reference drug, which was carried out at a temperature of 37 ℃ in an environment of 0.1M hydrochloric acid, showed that the introduction of simvastatin into the composition of the investigated self-emulsifying compositions allows to increase its solubility in this solvent by five times, compared to the reference drug.
 Conclusions. The obtained results indicate the effectiveness of the introduction of simvastatin into the composition of self-emulsifying drug delivery systems and the feasibility of using such systems to improve the solubility and accelerate the release of a poorly water-soluble active pharmaceutical ingredient into the gastric juice environment
EXPERIMENTAL RESEARCH ON THE DEVELOPMENT OF THE COMPOSITION OF THE TRANSDERMAL THERAPEUTIC SYSTEM OF ANTI-INFLAMMATORY ACTION BASED ON COMPOSITION OF NATURAL SUBSTANCES Liliia Vyshnevska, Anastasia Olefir, Dmytro Lytkin, Liubov Bodnar Sciencerise Pharmaceutical Science, 2022 The aim of the work is to develop the composition of the transdermal therapeutic system of anti-inflammatory action based on active pharmaceutical ingredients of natural origin. Materials and methods. Selection of adhesive materials, plasticizers and solvents, the effect of introduction to the base of active pharmaceutical ingredients, determination of optimal temperature and drying time, pharmacological screening was performed by physicochemical, biopharmaceutical and biological research methods. Results. The composition of the hydrophilic adhesive composition, which includes polyvinylpyrrolidone, eudragit (adhesives), PEO-400 (plasticizer), isopropyl alcohol (solvent) 50: 8: 12: 30, respectively. This composition has excellent organoleptic properties, is easily applied to the polymer base, holds its shape well (does not flow beyond the base), is applied in a thin layer and evenly distributed on the base. The optimal mode of drying of the adhesive composition based on PVP is 75 °C for 30 min, based on PVA – 75 °C for 35 min, the composition containing eudragit - 50 °C for 10 min. It is established that the addition of active substances to the adhesive composition does not adversely affect its properties. The study of antiexudative activity using different combinations of substances of natural origin as API and found that the best anti-inflammatory properties (25 %) have a sample No. 4, which includes dry extracts of white willow bark and sage leaves and quercetin 3:1:3 in accordance. Conclusions. Based on physicochemical, biopharmaceutical and pharmacological studies, the composition of the transdermal therapeutic system of anti-inflammatory action with active pharmaceutical ingredients of natural origin has been developed
Research on development of gel patch content for correction of skin age-related changes Pharmacologyonline, 2021
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